Leica DMLS Manual de usuario

MICROSYSTEMS

Leica DMLS

Instructions · Bedienungsanleitung

Mode d’emploi

Phone/Tel./Tél. +49 (0) 6441-290

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4th edition, issued in 2000 by/

4. Auflage, herausgegeben 2000 von/

4ème édition, publiée en 2000 par:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents/

Verantwortlich für den Inhalt/

Département responsable du contenu:

Marketing MQM, Product management

MICROSYSTEMS

Leica DM LS

Instructions

Copyrights

All rights to this documentation are owned by

Leica Microsystems Wetzlar GmbH. Copying of

text and illustrations – in full or in part – by print-

ing, photostat, microfilm or other techniques, in-

cluding electronic systems, is only permitted

subject to the express written consent of Leica

Microsystems Wetzlar GmbH.

The information contained in the following docu-

mentation represents the latest stage of tech-

nology and knowledge. We have composed the

texts and illustrations with great care. However,

as it is impossible to eliminate the risk of error

completely, we cannot accept any kind of liabil-

ity for the correctness of the contents of this

manual. Nevertheless, we are always grateful to

be notified of any errors.

The information in this manual may be altered

without prior notice.

Contents

Important notes on this manual ....................... 7

Assembly an description

of components ..................................................... 8

Site ......................................................................... 8

Mains voltage, fuses .......................................... 9

Assembly of components .................................. 10

Light sources, lamp change.............................. 16

Performance parameters .................................. 22

Objectives, eyepieces ........................................ 22

Tube system ......................................................... 25

Condensers .......................................................... 26

Operation .............................................................. 28

Basic setting for transmitted light ................... 28

Filters ..................................................................... 30

Condensers .......................................................... 30

Phase contrast .................................................... 34

Transmitted light darkfield................................. 36

Transmitted light polarization ........................... 37

Fluorescence ....................................................... 38

Linear measurements ........................................ 42

Thickness measurements ................................. 43

Object marker ...................................................... 44

TV microscopy ..................................................... 44

Care and maintenance ...................................... 46

Wearing and spare parts, tools ....................... 47

Supplementary information .............................. 48

Index ..................................................................... 52

EU Conformity declaration ............................... 53

General specifications

Main voltage: 230/115/100 V ±10 %

Frequency: 50 – 60 Hz ~

Power consumption: max. 40 W

For indoor use only!

Operating temperature: 10 – 36 °C

Relative humidity: 0 – 80 % to 30 °C

Overvoltage category: II

Contamination class: 2

Cross section diagramm of DAS Mikroskop Leica DM LS

12b

12a

18Aperture diaphragm

19Condenser lenses

10 Microscope stage with specimen

11 Objective

12 Tube lens system

13 Deflecting prisms in tube

14 Eyepiece(s)

1Coarse/fine focusing

2Halogen lamp

3Collector

4Field diaphragm

5Exit window in microscope base

6a Condenser lenses

6b Condenser clamp screw

7Auxiliary lens LS

Important notes on this manual

Attention:

This manual is an integral part of the product

and must be read carefully before switching

on and using the microscope!

It contains important instructions and infor-

mation for safe operation and maintenance of

the product and must therefore be kept in a

safe place!

Text symbols and their meanings:

(1.2) Numbers in brackets, e. g. (1.2) refer

to illustrations, in this example Fig. 1,

pos. 2.

→p. 20 Numbers with an arrow, e.g. →p. 20,

refer to a specific page in this manual.

Special safety information is

marked at the edge by the

lefthand symbol and highlighted

by a grey background.

This symbol means that incorrect

operation can damage the micro-

scope or its accessories.

Warning of hot surface.

Explanatory note.

* Item is not included in all variants of

the microscope.

The Leica DM L microscope series consists of

several basic stands and a range of modular

components allowing an almost unlimited

variety of individual outfits.

Therefore this manual has been given a modular

layout as well to show you other possible

configurations besides your own. It applies to

the microscope Leica DM LS and, together with

the supplementary DM LSP manual, to the

polarized light microscope Leica DM LSP.

This manual is devided into three main chapters:

Assembly, Performance parameters, Operation

The manual is multilingual. Due to the spiral

binding you can turn the language you want to

the front.

A foldable pocket-sized set of brief instructions

is also available in various languages for the

different basic stands, please consult your

supplier.

A list of optics with key data on objectives,

eyepieces, graticules and fluorescence filter

cubes is also supplied with this microscope. It is

constantly being updated.

Special manuals are delivered with some

additional equipment such as microscope

cameras, heating stages, and also in case of

modifications. We also print extensive

brochures on microscopy, which can be

ordered, as can extra copies of this manual,

from our agencies for a cover charge.

Assembly and description of components

Attention:

Do not connect the microscope and pe-

ripherals to the mains yet! (→p. 18 –21).

Installation site

Attention:

Make sure that the workplace is free from oil

and chemical fumes. Vibrations, direct sunlight

and major temperature deviations have a

negative effect on measurements and photo-

micrography. It is important to have a stable

desk of the right height (70 –80 cm). This and an

ergonomically designed chair which can be

adjusted in several positions are the basic

prerequisites for fatigue-free microscopy.

Unpacking Documents

Please compare the delivery carefully with the

packing note, delivery note or invoice. We

strongly recommend that you keep a copy of

these documents with the manual, so that you

have information on the time and scope of

delivery later when ordering more equipment or

when the microscope is serviced. Make sure

that no small parts are left in the packing

material. Some of our packing material has

symbols indicating environmental-friendly re-

cycling.

Attention:

Important note! When taking the microscope out

of its packing and putting it onto the desk take

care not to damage the sensitive vibration-

damping feet on the bottom of the microscope.

Fig. 1 Assembly tools

13 mm Allen key

2.5 mm Allen key (short)*

31.5 mm centering keys*

2 mm centering keys*

for UCL/UCLP condenser

5Crosstip screwdriver*

Fig. 2 The main microscope

components

* not part of all outfits

Eyepieces

Tube

Intermediate system

Objective

nosepiece

Object

stage

Condenser

Micro-

scope

stand

Base

Light source

Assembly tools

You only need a few ordinary screwdrivers to

assemble your microscope. These are supplied

with the delivery. Replacements for lost tools

can be obtained from us or from a tool shop

(Fig. 1, see list of spare parts, →p. 49).

Setting the mains voltage

Attention:

Make sure to check the voltage setting (230

or 115 or 100 V) on the back of the microscope

(3.6) and correct if necessary:

Do not forget to disconnect from the mains

(3.2)!

n. b.! The 100 V setting must not be used for

115 V!

Release the lock button (3.5) by pressure with a

biro or pen and remove the fuse holder (3.4). Pull

out the square module (3.6) and replace it so

that the number of the desired mains voltage

appears in the window (upside down).

Push fuse holder (3.4) back in until you hear the

locking button (3.5) click into position.

Fuses

The two mains fuses (see spare parts list on

p. 47, identical for all mains voltages) can be

accessed after pressing the lock button (see 3.5,

mains voltage).

Attention:

Never use other types of fuses!

Attention:

If using external lamp power units, always

set the mains voltage as instructed in the

special manual or use a series transformer,

e. g. 115/230 V.

Fig. 3 Fuses and mains

adaption

1Nameplate

2Mains connection

3Mains fuses (2)

4Fuse holder with window

showing mains voltage

5Lock button

6Module for voltage setting

Fig. 4 Underneath the stand

→Provision for ground

connection

Safety

Attention:

To ensure that the microscope and

accessories are in a perfectly safe condition,

please note the following advice and

warnings: The mains plug must only be

inserted into a grounded outlet. If an ex-

tension cord is used, it must be grounded as

well. Using the connection on the base plate

(Fig. 4), any accessories connected to the

microscope which have their own and/or a

different power supply can be given the

same ground conductor potential. Please

consult our servicing personnel if you intend

to connect units without a ground conductor.

Attention:

The instruments and accessory components

described in the manual have been tested

for safety or possible hazards. It is essential

to consult your Leica agency or the main

factory in Wetzlar before carrying out any

operations on the instrument, modifications,

or combination with non-Leica components

not dealt with in this manual.

Transit protection

Attention:

The sensitive focus drive is only automatically

protected from damage during transport in its

original packing. If the packing is no longer

available or greatly damaged, the vertical stage

movement must be blocked by putting hard foam

rubber padding above and below the stage for

longer periods of transport. Objectives,

condenser, tube and intermediate systems

should be disassembled.

Tubes and intermediate systems

The tube is adapted to the stand direct (Fig. 23)

or via mediate systems (Fig. 31). Tubes and

intermediate systems are secured with the

lateral clamp screw (27.3):

Loosen clamp screw (27.3) slightly if necessary

with Allen key (1.1). Insert the tube or

intermediate system into the circular mount

(dovetail) and align by rotating (viewing port to

the front). Pol components may have a clickstop

device (pin).

Attention:

Make sure that components do not jam each

other. Retighten clamp screw (27.3).

When combined with other intermediate

systems, the fluorescence illuminator (Fig. 31)

is always assembled underneath (i. e. directly

onto the microscope stand). The number and

type of usable intermediate systems is limited,

→p. 25 –26.

Besides tubes from the DM L range (Fig. 35), it is

also possible to adapt tubes from DM R research

microscopes (Fig. 36) using the R/L adapter

(36.2).

The Ergo module (36.3) is for raising the viewing

port by 30 mm (or 60 mm if two are used).

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