Hanna Instruments PH 211R Manual de usuario

Instruction Manual

pH211R

Microprocessor-based

Bench pH/mV/°C Meter

with Analog Output

www.hannainst.com

PRELIMINARY EXAMINATION

Dear Customer,

Thank you for choosing a Hanna Instruments Product.

Please read this instruction manual carefully before using the instru-

ment.

This manual will provide you with all the necessary information for

correct use of the instrument, as well as a precise idea of its versatility

in a wide range of applications.

If you need additional technical information, do not hesitate to e-mail

us at [email protected].

This instrument is in compliance with the directives.

The Hanna pH 211R is an advanced microprocessor based bench pH

meter easy to operate, perfect for quality control laboratories.

pH 211R also measures in the temperature and mV ranges.

Temperature compensation is automatic thanks to the HI 7669/2W

temperature probe supplied with the instrument. If necessary the user

can also set temperature compensation manually.

The large display shows pH (or mV) and temperature values

simultaneously.

The user is guided through the calibration process with simple

instructions on the display.

The meter also features 0 to 5 V analog output.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION......................................................... 3

GENERAL DESCRIPTION ................................................................ 3

FUNCTIONAL DESCRIPTION ........................................................... 4

SPECIFICATIONS .......................................................................... 5

OPERATIONAL GUIDE ................................................................... 6

pH CALIBRATION.......................................................................... 9

ELECTRODE CONDITIONING & MAINTENANCE............................... 12

TEMPERATURE-RESISTANCE CORRELATION

FOR pH SENSITIVE GLASS .......................................................... 15

TROUBLESHOOTING GUIDE......................................................... 16

ACCESSORIES ............................................................................ 17

WARRANTY ................................................................................ 23

GENERAL DESCRIPTION

Remove the instrument from the packing material and examine it

carefully to make sure that no damage has occurred during shipping.

If there is any damage, notify your Dealer.

Each meter is supplied with:

•HI 1131B glass body pH electrode with BNC and 1 m cable

•HI 7669/2W temperature probe

•HI 76404 electrode holder

• pH 4.01 & pH 7.01 buffer solutions (20 mL each)

•HI 7071S electrolyte solution

• 12 Vdc power adapter

• Instruction manual

Note: Save all packing material until you are sure that the

instrument functions correctly. Any defective items must be

returned in the original packing with the supplied accessories.

FUNCTIONAL DESCRIPTION

1) Liquid Crystal Display (LCD)

2) CFM key, to confirm calibration values

3) CAL key, to enter or exit calibration mode

4) ▼°C key, to manually decrease temperature or select pH buffer

5) ▲°C key, to manually increase temperature or select pH buffer

6) MR key, to recall the stored value

7) MEM key, to store a value in memory

8) RANGE key, to select measurement range

9) ON/OFF button, to switch the meter on and off

10) Socket for power adapter

11) BNC connector for measurement electrode

12) Reference electrode connector

13) Temperature probe socket

14) RESET button

15) Positive (+) analog output connector

16) Negative (-) analog output connector

SPECIFICATIONS

Range -2.00 to 16.00 pH

±399.9 / ±2000 mV

-9.9 to 120.0°C

Resolution 0.01 pH

0.1 / 1 mV

0.1°C

Accuracy (@20°C/68°F) ±0.01 pH

±0.2 / ±1 mV

±0.5°C

Typical EMC Deviation ±0.03 pH

±2 mV / ±0.3°C

pH Calibration Automatic, 1 or 2 point

with 5 memorized buffers (pH 4.01, 6.86, 7.01, 9.18, 10.01)

Temperature Compensation

Automatic (with HI 7669/2W probe) or manual

-9.9 to 120°C (14 to 248°F)

pH Electrode HI 1131B glass body, double junction, refillable,

BNC connector and 1 m cable (included)

Temperature Probe HI 7669/2W (included)

Input Impedance 1012 Ω

Analog Output 0 to 5 VCC not isolated

Analog Output Accuracy ±10 mV

Power supply 12 Vdc adapter (included)

Environment 0 to 50°C (32 to 122°F); RH max 95%

Dimensions 240 x 182 x 74 mm (9.4 x 7.1 x 2.9")

Weight 1.1 kg (2.4 lb.)

If measurements are taken successively in different samples, it is

recommended to rinse the electrode thoroughly with water and then

with some of the next sample to be measured.

pH readings are affected by temperature. In order for the meter to

measure the pH accurately, temperature must be taken into consid-

eration. To use the Automatic Temperature Compensation (ATC)

feature, submerge the HI 7669/2W temperature probe into the

sample as close to the electrode as possible and wait for a couple of

minutes.

If the temperature of the sample is known or tests are always

performed at the same temperature, you can simply manually

compensate for it. For this purpose the temperature probe must be

disconnected.

The display will then show the default tem-

perature of 25°C with the "°C" symbol blinking.

Note the temperature of the sample using a

ChecktempC (HI 98501) or another accurate

thermometer as reference.

The temperature can now be adjusted with the ▲°C and ▼°C keys.

TAKING ORP MEASUREMENTS

An optional ORP electrode must be used to perform ORP measure-

ments (see accessories).

Oxidation-reduction potential (REDOX) measurements provide the

quantification of the oxidizing or reducing power of the sample tested.

To correctly perform a redox measurement, the surface of the ORP

electrode must be clean and smooth.

OPERATIONAL GUIDE

Power Connection

Plug the 12 Vdc adapter into the power supply socket.

Note: Make sure the mains line is protected by a fuse.

Electrode and Probe Connections

Connect the BNC of the pH or ORP electrode to the BNC socket on the

rear panel.

For electrodes with a separate reference, connect the reference elec-

trode plug to the reference socket (#12 on page 4).

For temperature measurements and automatic temperature compen-

sation, connect the temperature probe to the appropriate socket.

Note: To prevent damage to the pH electrode, remove it from the

solution before turning the meter off.

With the meter OFF, detach the electrode from the meter and

immerse it in the storage solution.

TAKING pH MEASUREMENTS

Make sure that the instrument has been calibrated for pH before

taking pH measurements.

• Switch the instrument on by pressing the

ON button. The meter automatically de-

faults to the pH measurement mode.

• Submerge the tip (4 cm / 1½") of the

electrode and temperature probe into the

sample to be tested. Allow a couple of

minutes for the electrode to stabilize.

• The pH reading is displayed on the primary LCD and temperature

on the secondary one.

• To view the mV reading, press the RANGE key.

4 cm

(1½")

• Switch the meter on and press the RANGE

key to enter the mV mode.

• Submerge the ORP electrode tip (4 cm /

1½") into the sample. Allow a few minutes

for the reading to stabilize.

• The meter displays the mV reading on the primary LCD.

• If reading is out of range, "---" will appear on the LCD.

TAKING TEMPERATURE MEASUREMENTS

Connect the HI 7669/2W temperature probe

to the proper socket on the rear panel and

turn the instrument on. Dip the temperature

probe in the sample and allow the reading to

stabilize.

HOLD FUNCTION

Press and hold the MEM key. The last reading will be frozen on the

display until MEM is released. During this operation, the MEM

indicator is also shown on the LCD.

Press MR to recall the previously memorized

reading. The value will be displayed with the

MEM indicator on the LCD.

4 cm

(1½")

OUTPUT RECORDER FUNCTION

Connect the recorder to the analog output socket and turn the

instrument ON. The value on the analog output will be related with

the pH or mV value displayed on the LCD as follows:

pH: 0 7 14

mV: -2000 0 2000

analog output: 0 V 2.5 V 5 V

RESET

The RESET button (#14 on page 4) should only be used when the

instrument displays erroneous messages due to strong electrical inter-

ference or when the power supply was disconnected before switching

the meter off.

After pressing RESET, always recelibrate the unit before proceeding.

pH CALIBRATION

Frequent calibration is recommended. Moreover, the instrument should

be re-calibrated for pH:

a) whenever the pH electrode is replaced

b) at least once a week

c) after testing aggressive chemicals

d) if higher accuracy is required

e) after the RESET button has been pressed

PREPARATION

Pour small quantities of the buffer solutions into clean beakers. If

possible use plastic beakers to minimize any EMC interferences.

For accurate calibration and to minimize cross contamination use two

beakers for each buffer solution. One for rinsing the electrode and the

second for calibration.

If you are measuring in the acid range, use pH 4.01 as second

buffer; if you are measuring in the alkaline range, use pH 10.01 or

pH 9.18 as second buffer.

PROCEDURE

Five memorized buffer values are available for calibration: pH 4.01,

6.86, 7.01, 9.18 and 10.01.

Calibration can be performed at 1 or 2 points. For best accuracy, a 2

point calibration is recommended.

1110

•Press CAL to quit calibration. The meter

will save the one-point calibration data

and return to normal mode.

Two-Point Calibration

Any two of the 5 memorized buffers can be used for this purpose.

However, it is recommended to choose pH 6.86 or 7.01 as the first

point and pH 4.01 (acidic sample) or pH 9.18/10.01 (alkaline

environment) as the second point.

•After the first calibration point is con-

firmed, immerse pH electrode and

temperature probe approx. 4 cm (1½") in

the second buffer solution and stir gently.

The temperature probe should be close to

the pH electrode.

•If necessary, press ▲°C or ▼°C to select a

different buffer value.

Note: The meter will automatically skip the buffer used for the first

point. It also skips pH 6.86 if 7.01 was used, and viceversa,

and pH 9.18 if 10.01 was used, and viceversa.

•The "NOT READY" indication will blink on

LCD until the reading has stabilized.

•When the reading is stable, "READY" and "CFM" will blink. Press

CFM to confirm the calibration.

•If the reading is close to the selected buffer, the meter stores the

calibration point, displays the "Stor" message for 1 second and

returns to normal operational mode.

•If the measured value is not close to the

selected buffer, "WRONG " and "WRONG

" will blink alternately.

In this case check if the correct buffer has been used, or refresh

the electrode by following the maintenance procedure (see "Elec-

trode Maintenance" section). If necessary change buffer or electrode.

Note: Press RANGE to display the temperature reading on the LCD

during calibration.

One-Point Calibration

•Immerse pH electrode and temperature

probe approximately 4 cm (1½") into

any of the available buffer solutions (pH

4.01, 6.86, 7.01, 9.18 or 10.01) and

stir gently. The temperature probe should

be close to the pH electrode.

•Press CAL. The "CAL" and " " indicators, and "7.01" will be

displayed.

Note: The buffer value displayed on the secondary LCD, varies with

temperature. For example, at 20ºC it shows 4.00, 7.03 and

10.06, while at 25ºC it shows 4.01, 7.01 and 10.01.

•If necessary, press ▲°C or ▼°C to select

a different buffer value.

•The "NOT READY" indication will blink on

the LCD until the reading has stabilized.

•When the reading is stable, "READY" and "CFM" will blink. Press

CFM to confirm the calibration.

•If the reading is close to the selected buffer value, the meter stores

the calibration point. The "Stor" message appears on the LCD for

1 second, then the buffer value is displayed on the primary LCD

and the secondary LCD displays the second expected buffer value.

•If the measured value is not close to the

selected buffer, the "WRONG " and

"WRONG " symbols will blink alter-

nately.

In this case check if the correct buffer has been used or refresh the

electrode by following the maintenance procedure (see "Electrode

Maintenance" section). If necessary change the buffer or the

electrode.

4 cm

(1½")

4 cm

(1½")

1312

For refillable electrodes

If the filling solution (electrolyte) is more than 2.5 cm (1") below the

fill hole, add HI 7082 3.5M KCl electrolyte solution for double junction

or HI 7071 3.5M KCl+AgCl electrolyte solution for single junction

electrodes.

For a faster response, unscrew the fill hole screw during measure-

ments.

For AmpHel®

electrodes

If the electrode does not respond to pH changes, the battery is run

down and the electrode should be replaced.

MEASUREMENT

Rinse the electrode tip with distilled water. Immerse the tip (bottom 4

cm /1½") in the sample and stir gently for a few seconds.

For a faster response and to avoid cross contamination of the samples,

rinse the electrode tip with a few drops of the solution to be tested,

before taking measurements.

STORAGE

To minimize clogging and assure a quick response time, the glass bulb

and the junction should be kept moist and not allowed to dry out.

Replace the solution in the protective cap with a few drops of

HI 70300 storage solution or, in its absence, filling solution (HI 7071

for single junction or HI 7082 for double junction electrodes). Follow

the Preparation Procedure above before taking measurements.

Note: NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.

PERIODIC MAINTENANCE

Inspect the electrode and the cable. The cable used for connection to

the meter must be intact and there must be no points of broken

insulation on the cable or cracks on the electrode stem or bulb.

Connectors must be perfectly clean and dry. If any scratches or cracks

are present, replace the electrode. Rinse off any salt deposits with

water.

For refillable electrodes:

Refill the reference chamber with fresh electrolyte (HI 7071 for single

junction or HI 7082 for double junction electrodes). Allow the elec-

trode to stand upright for 1 hour.

Follow the Storage Procedure above.

AmpHel®is a registered Trademark of "Hanna Instruments"

ELECTRODE CONDITIONING & MAINTENANCE

PREPARATION

Remove the protective cap.

DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal

with electrodes and they will disappear when rinsed with water.

During transport tiny bubbles of air may form inside the glass bulb

affecting proper functioning of the electrode. These bubbles can be

removed by "shaking down" the electrode as you would do with a

glass thermometer.

If the bulb and/or junction is dry, soak the electrode in HI 70300

storage solution for at least one hour.

Note: To prevent damage to the pH electrode, remove it from the

solution before turning the meter off.

With the meter OFF, detach the electrode from the instrument

and immerse it in the storage solution.

1514

TEMPERATURE-RESISTANCE CORRELATION FOR

HANNA pH SENSITIVE GLASS

The resistance of glass electrodes partially depends on the temperature.

The lower the temperature, the higher the resistance. It takes longer time

for the reading to stabilize if the resistance is higher. In addition, the

response time will suffer to a greater degree at temperatures below 10°C.

Since the resistance of the pH electrode is in the range of 200 Mohm,

the current across the membrane is in the pico Ampere range. Large

currents can disturb the calibration of the electrode for many hours.

For these reasons high humidity environments, short circuits and

static discharges are detrimental to a stable pH reading.

The pH electrode's life also depends on the temperature. If constantly

used at high temperatures, the electrode life is drastically reduced.

Typical Electrode Life

Ambient Temperature 1- 3 years

90 °C Less than 4 months

120°C Less than 1 month

High concentrations of sodium ions interfere with readings in alkaline

solutions; the pH at which the interference starts to be significant

depends upon the composition of the glass. This interference is the

alkaline error and causes the pH to be underestimated. Hanna's glass

formulations have the indicated characteristics.

CLEANING PROCEDURE

General Soak in Hanna HI 7061 general cleaning solution for

approximately 30 minutes.

Removal of films, dirt or deposits on the membrane/junction:

- Protein Soak in Hanna HI 7073 protein cleaning solution for

15 minutes.

- Inorganic Soak in Hanna HI 7074 inorganic cleaning solution

for 15 minutes.

- Oil/grease Rinse with HI 7077 Oil & Fat cleaning solution.

IMPORTANT: After performing any of the cleaning procedures rinse

the electrode thoroughly with distilled water, refill the reference cham-

ber with fresh electrolyte (not necessary for gel-filled electrodes) and

soak the electrode in HI 70300 storage solution for at least 1 hour

before taking measurements.

TROUBLESHOOTING

Evaluate the performance of your electrode based on the following.

•Noise (readings fluctuate up and down) could be due to:

– Clogged/Dirty Junction: refer to the Cleaning Procedure.

– Loss of shielding due to low electrolyte level (in refillable

electrodes only): refill with fresh HI 7071 for single junction or

HI 7082 for double junction electrodes.

•Dry Membrane/Junction: soak in HI 70300 storage solution for

at least 1 hour.

•Drifting: soak the electrode tip in warm (approx. 50-60°C)

Hanna HI 7082 solution for one hour and rinse the tip with

distilled water. Refill with fresh HI 7071 for single junction

electrodes and HI 7082 for double junction electrodes (refillable

electrodes only).

•Low Slope: refer to the cleaning procedure above.

•No Slope: check the electrode for cracks in glass stem or bulb

and replace the electrode.

•Slow Response/Excessive Drift: soak the tip in HI 7061

solution for 30 minutes, rinse thoroughly in distilled water and

then follow the Cleaning Procedure above.

-2

-10

Ω

-10

Ω

2x109

1x109

2x108

1x108

2x107

1x107

-20 -10 0 +10 +20+30+40 +50 +60 +70 +80 +90 °C

Alkaline Error

1.0 Mol L-1 Na+

0.1 Mol L-1 Na+

Sodium Ion Correction for the Glass at 20-25°C

Concentration pH Error

13.00

13.50

14.00

12.50

13.00

13.50

14.00

0.10

0.14

0.20

0.10

0.18

0.29

0.40

1716

ACCESSORIES

pH CALIBRATION SOLUTIONS

HI 70004P pH 4.01 buffer solution, 20 mL sachet, 25 pcs

HI 70007P pH 7.01 buffer solution, 20 mL sachet, 25 pcs

HI 70010P pH 10.01 buffer solution, 20 mL sachet, 25 pcs

HI 7004L pH 4.01 buffer solution, 500 mL bottle

HI 7006L pH 6.86 buffer solution, 500 mL bottle

HI 7007L pH 7.01 buffer solution, 500 mL bottle

HI 7009L pH 9.18 buffer solution, 500 mL bottle

HI 7010L pH 10.01 buffer solution, 500 mL bottle

HI 8004L pH 4.01 buffer solution in FDA bottle, 500 mL

HI 8006L pH 6.86 buffer solution in FDA bottle, 500 mL

HI 8007L pH 7.01 buffer solution in FDA bottle, 500 mL

HI 8009L pH 9.18 buffer solution in FDA bottle, 500 mL

HI 8010L pH 10.01 buffer solution in FDA bottle, 500 mL

ELECTRODE MAINTENANCE SOLUTIONS

HI 70300L Storage solution, 500 mL bottle

HI 80300L Storage solution in FDA bottle, 500 mL

HI 70000P Rinsing solution, 20 mL sachet, 25 pcs

HI 7061L General cleaning solution, 500 mL bottle

HI 8061L General cleaning solution in FDA bottle, 500 mL

HI 7073L Protein cleaning solution, 500 mL bottle

HI 8073L Protein cleaning solution in FDA bottle, 500 mL

HI 7074L Inorganic cleaning solution, 500 mL bottle

HI 7077L Oil & Fat cleaning solution, 500 mL bottle

HI 8077L Oil & Fat cleaning solution in FDA bottle, 500 mL

HI 7071 3.5M KCl+AgCl electrolyte solution, 4 x 50 mL bottle,

for single junction electrodes

HI 8071 3.5M KCl+AgCl electrolyte solution in FDA bottle, 4 x 50

mL, for single junction electrodes

HI 7072 1M KNO3electrolyte solution, 4 x 50 mL bottle

HI 8072 1M KNO3electrolyte solution in FDA bottle, 4 x 50 mL

HI 7082 3.5M KCl electrolyte solution 4 x 50 mL bottle, for

double junction electrodes

HI 8082 3.5M KCl electrolyte solution in FDA bottle, 4 x 50 mL,

for double junction electrodes

TROUBLESHOOTING GUIDE

Symptoms Problem Solution

The meter is slow in

responding or gives

faulty readings

The meter does not ac-

cept the 2nd buffer

solution for calibration

The reading drifts

Display shows:

"WRONG "

and

"WRONG "

Display shows:

"WRONG "

and

"WRONG "

The meter does not

work with the tempera-

ture probe

The meter fails to cali-

brate or gives faulty

readings

The electrode is not work-

ing or the reference

junction is clogged

Out of order pH electrode

Defective pH electrode

Out of range pH scale

Out of range tempera-

ture scale

Out of range mV scale

Erroneous buffer solution

used for offset calibra-

tion

Defective electrode

Erroneous buffer solution

used for slope calibration

Defective electrode

Out of order temperature

probe

Out of order pH electrode

Leave the electrode in a stor-

age solution after cleaning

the junction. If problem per-

sists, replace the electrode

Follow the cleaning proce-

dure. If this doesn't work

replace the electrode

Replace the electrode

a) Recalibrate

b) Make sure the pH sample

is in the 0 to 14 range

c) Check the electrolyte level

and the general state of

the pH electrode

Make sure the temperature

is in the 0 to 100°C range

and the temperature probe is

plugged in

Electrode not connected

Make sure the buffer setting

is correct and the solution is

fresh. Replace the buffer if

necessary

Replace the electrode

Make sure the buffer setting

is correct and the solution is

fresh. Replace the buffer if

necessary

Replace the electrode

Replace the probe

Replace the electrode

1918

HI 1330B

Glass body, semimicro, single junction, refillable, combination pH

electrode. Use: laboratory, vials.

HI 1331B

Glass body, semimicro, single junction, refillable, combination pH

electrode. Use: flasks.

HI 1230B

Plastic body, double junction, gel-filled, combination pH electrode.

Use: general, field.

HI 2031B

Glass body, semimicro, conic, refillable, combination pH electrode.

Use: semisolid products.

HI 1332B

Plastic body, double junction, refillable, combination pH electrode.

Use: general purpose.

120 mm

4.7"

5mm

0.2"

5mm DIA

0.2"

"S" VERSION

HI 1330

120 mm

4.7"

12 mm

0.5"

VERSION

HI 1230

75 mm

6 mm

0.25"

VERSION

HI 2031

120 mm

4.7"

12 mm

0.5"

"S" VERSION

HI 1332

210 mm

8.25"

8 mm

0.3" 7.5mm DIA

0.29"

"S" VERSION

HI 1331

ORP PRETREATMENT SOLUTIONS

HI 7091L Reducing pretreatment solution, 500 mL bottle

HI 7092L Oxidizing pretreatment solution, 500 mL bottle

pH ELECTRODES

All electrode part numbers ending in B are supplied with BNC

connector and 1 m (3.3') cable.

HI 1043B

Glass body, double junction, refillable, combination pH electrode.

Use: strong acid/alkali.

HI 1053B

Glass body, triple ceramic, conic shape, refillable, combination pH

electrode. Use: emulsions.

HI 1083B

Glass body, micro, Viscolene, non-refillable, combination pH electrode.

Use: biotechnology, micro titration.

HI 1131B

Glass body, single junction, refillable, combination pH electrode.

Use: general purpose.

120 mm

4.7"

12 mm

0.5"

9.5mm DIA

0.37"

"S" VERSION

HI 1043

120 mm

4.7"

12 mm

0.5"

"S" VERSION

HI 1053

120 mm

12 mm

0.5" 5 mm

0.2" 3 mm

0.12" 3.0 mm DIA

0.12"

HI 1083

120 mm

4.7"

12 mm

0.5"

9.5mm DIA

0.37"

"S" VERSION

HI 1131

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