Gilson PLATEMASTER P20 Manual de usuario

User’s Guide

TABLE OF CONTENTS

TABLE OF CONTENTS | PLATEMASTER®

TABLE OF CONTENTS

INTRODUCTION | 3

Unpacking | 4

Safety | 4

INSTALLATION | 5

General View | 6

Technical Data | 6

OPERATION | 7

Description | 7

Moving PLATEMASTER | 7

Height Adjuster | 7

Volume Setting | 8

Tip Fitting | 8

Pipetting | 9

Pipetting 384 wells | 10

Tip Ejection | 11

General Guidelines for Good Pipetting | 12

ACCESSORIES | 13

ASSOCIATED TIPS | 14

SERVICE | 15

GLP Features | 15

Cleaning and Decontamination | 15

Material in Contact with Liquids or Vapors | 15

Use the Lubrication Box | 15

SPECIFICATIONS | 16

WARRANTY | 17

Chapter 1

INTRODUCTION

PLATEMASTER® is an easy-to-use, accurate solution for high-throughput manual

pipetting of 96- and 384-well microplates.

PLATEMASTER’s 96-channel design greatly reduces the number of pipetting steps

necessary to ﬁll a microplate when compared to using regular manual multichannel

pipettes. When using PLATEMASTER, the time it takes to ﬁll 96-well plates is

signiﬁcantly reduced to approximately 10-20 seconds or less, and 384 wells can

typically be ﬁlled in less than a minute using only four pipetting steps.

Figure 1

Gilson PLATEMASTER

P20

PLATEMASTER is suitable for a wide range of applications:

• Pipetting and providing master mixes for PCR/qPCR

• Simultaneous processing of nearly all reaction steps in

microplates (coating, starting, stopping, washing, etc.) for

applications such as ELISA

• Addition and removal of culture media

• Plate replication in cell culture

• Dropwise addition of solutions for

crystallography

• Plate replication in expression tests

• Enhancement of existing automation:

assay development and adaptation to

96-channel systems

• Back-up solution for maintenance and

downtime

• Workflows of automated systems

involving pipetting on a 96-well or

384-well scale

INTRODUCTION

PLATEMASTER®|USER’S GUIDE

Safety

PLATEMASTER is designed for research purposes only. If PLATEMASTER is used in

a manner not specified by Gilson, the protection provided by the instrument may be

impaired. Gilson will not be responsible for damages.

For all transportation, Gilson recommends removing the pipetting head from the base

unit and using the original shipping box. For all moving, the head must be locked with

the secure lock (refer to Moving PLATEMASTER on page 7).

For disassembly, please reverse the installation procedure (refer to INSTALLATION on

page 5).

Temperature of use: 4°C to 40°C - Recommended working temperature: 20°C to 25°C

Use only the accessories provided.

Gilson denies any responsibility for service and repairs carried out by the customer

or third-party companies. Neither the equipment nor the filter tips installed can be

autoclaved.

Gilson is not liable for any damage resulting from the use of PLATEMASTER.

Follow the instructions in this user’s guide to ensure durability and top performance of

your PLATEMASTER. Keep this manual handy for future reference, in accordance with

good laboratory practices.

Unpacking

Each PLATEMASTER configuration is shipped in one box.

PLATEMASTER P220

(part number F110762) contains:

• PLATEMASTER P220

• User’s guide

• 3 height adjusters

• 1 lubrication box

• 1 Allen ball head wrench 3.0 x

100 mm (for assembling and

disassembling of the pipetting

head)

• 1 transport box and packing

material

• Gilson original certificate of

conformity

PLATEMASTER P20

(part number F110761) contains:

• PLATEMASTER P20

• User’s guide

• 3 height adjusters

• 1 lubrication box

• 1 Allen ball head wrench 3.0 x

100 mm (for assembling and

disassembling of the pipetting head)

• 1 transport box and packing material

• Gilson original certificate of

conformity

INTRODUCTION

4INTRODUCTION | PLATEMASTER®

Chapter 2

INSTALLATION

Read this section carefully before installing and operating PLATEMASTER.

The instrument described in this user’s guide should only be operated by qualified

personnel in a laboratory or similar indoor environment.

Cleaning, installation, dismantling, maintenance, adjustment, and repair should be

performed by appropriately trained personnel aware of the hazards involved.

If a spill occurs, refer to the material safety data sheet (MSDS) provided by the

chemical manufacturer before cleaning up the spill and take all safety precautions

required.

Remove PLATEMASTER pipetting head and PLATEMASTER base from box.

Insert the pipetting head as follows and secure the head by screwing the first screw

down.

Figure 2

Unpacking PLATEMASTER® head and base

Figure 3

Location studs

onhead

Figure 4

Sockets for location

studs on base

Figure 5

Insert the location

studs into the sockets

Figure 6

Pull the head down a little and turn the screw

to the lower stop

Keep the packaging!

It will be useful for sending the device for any maintenance and

calibration.

NOTE

INSTALLATION

PLATEMASTER®|USER’S GUIDE

General View

The figure below shows a general view of a PLATEMASTER.

Pipetting head

Volume adjustment wheel

Volume display

Tip fitting handle

Pipetting handle

Figure 7

PLATEMASTER

parts

Technical Data

MODEL PLATEMASTER P20 PLATEMASTER P220

Part Number F110761 F110762

Volume Range 0.5 to 20 µL

Volume can be adjusted

in 0.1 µL increments

2 to 220 µL

Volume can be adjusted

in 1 µL increments

Temperature

Range Recommended working temperature:

20°C to 25°C / 68°F to 77°F

Dimensions 300 x 450 x 400 mm / 12 x 18 x 16 Inch (W x L x H)

Weight approx. 16 kg / approx. 35 lbs.

Tip ejector

Movable drawers

Pipetting head height adjuster

Pin-plate

INSTALLATION

6INSTALLATION | PLATEMASTER®

Chapter 3

OPERATION

Description

The pipetting head moves both vertically and horizontally. Vertical movements

are made to fit tips and pipette liquid. Horizontal movements are made to

transfer liquids. This movement is possible only when the head is fully raised.

Two drawers can hold up to three different microplates and can be used at the

same time.

Height Adjuster

The descent of the pipetting head and the

position of the tips can be adjusted by using

three height adjusters, as shown in figure 9. .

This will prevent the tips from touching

the bottom of the microplate and can be

adjusted for use with shallow- or deep-well

microplates.

Figure 8

PLATEMASTER®lock

Figure 9

Three height adjusters positions

Tip ejector

Movable drawers

Pipetting head height adjuster

Pin-plate

Moving PLATEMASTER

Whether placed on the lab bench, under a fume hood,

in a cold room, or used in the field, PLATEMASTER is

completely portable. PLATEMASTER includes a locking

system to lock the head when the instrument is being

moved.

After each use of PLATEMASTER, remember to lock the

pipetting head to prepare for transport.

To lock the pipetting head, pull the black part on the

left side of PLATEMASTER, lower the head, and turn

the black part until the notch is in the slot. Let the head

slowly ride up and be locked.

To unlock the pipetting head, pull the black part and turn it. Once the pipetting head

is released, check proper movement of the device.

Never move the device without locking the pipetting head.

Damages caused by movement without locking the device will not

be covered by the warranty.

NOTICE

OPERATION

PLATEMASTER®|USER’S GUIDE

Volume Setting

Do not adjust the volume without squeezing both parts of the pipetting handle

The volume of liquid to be aspirated is set

using the volume adjustment wheel

With the pipetting handle fully squeezed, the

volume is set by slowly turning the volume

adjustment wheel to reach the required

setting.

Never try to force the volume outside of the

volume range permitted by the device.

Tip Fitting

For optimum performance, use of PIPETMAN® DIAMOND Tips or AmpliPur® Expert

Tips with PLATEMASTER is strongly recommended. Plastic tips are for a single use and

should not be cleaned for reuse.

To fit PIPETMAN DIAMOND Tips or AmpliPur Expert Tips on PLATEMASTER:

• Raise the pipetting head vertically using

the pipetting handle. Move the pipetting

head to the left side.

• Slide the right drawer out and place

anew tip rack on the back position.

The four locations have been specially

designed for PIPETMAN DIAMOND

Tips TIPACK™. Slots embedded in the

drawers ensure that tip racks securely

fit into all locations.

• Use the pipetting handle to position

the pipetting head to the right and

lower it far enough so that a contact is

made with the tips in the rack.

• Press down on the tip fitting handle with moderate pressure. The tip ejector

must be fully expanded to ensure that the tips are fully attached .

• Move the pipetting head up and the right drawer back.

The device is now ready to pipette liquid.

1 2

Before using AmpliPur® Expert Tips, conﬁrm that you have the

AmpliPur Expert Tips pin-plate assembly (F110765) and AmpliPur

Expert Tips reload block (F110766) installed on PLATEMASTER.

NOTE

OPERATION

8OPERATION | PLATEMASTER®

Pipetting

Place a vessel containing the solution to be pipetted in the front location of the right

drawer. The solution should be under the pipetting head.

Liquids containing protein solutions and organic solvents can leave a film of liquid

on the inside wall of the tip; pre-rinse the tip to minimize any errors related to

this phenomenon. Pre-rinsing consists of aspirating the first volume of liquid and

then dispensing it back into the same vessel (or into a waste basket). Subsequent

volumes pipetted will have levels of accuracy and precision within specifications. As

recommended by the ISO 8655 standard, pre-rinse to stabilize dead volume inside

the pipetting head

ASPIRATION

• Gently squeeze the pipetting handle to the first stop (this corresponds to the

set volume of liquid).

• Use the pipetting handle to lower

the pipetting head to immerse the

tips in the liquid (see immersion depth

table below). It is important that the

tips do not touch the bottoms of the

wells.

• Slowly retract the pipetting handle to

aspirate the liquid into the tips.

• Move the pipetting head fully up.

To simplify this operation, the height

adjuster can be set by turning the

upper screw so that the pipetting head

may only be moved down to a position

slightly above the bottom.

MODEL IMMERSION DEPTH

(MILLIMETERS)

WAIT TIME

(SECONDS)

P20 2-3 1

P220 2-4 1

Check the tip ejector when ﬁtting tips. If the tips have shifted this

implies that they have not been ﬁtted correctly.

The maximum

immersion depth

of the tips can be

adjusted using a

height adjuster.

NOTE

OPERATION

PLATEMASTER®|USER’S GUIDE

DISPENSING

• Place a 96-well microplate at the front location of the left drawer and keep it

fully retracted in the rear position.

• Move the pipetting head to the left and align the tips with the microplate.

• Pull the pipetting head slowly down into

the wells, but do not touch the bottom

of the wells.

• Squeeze the pipetting handle

slowly to dispense all the solution.

• Pull the pipetting head slowly up with

the pipetting handle fully squeezed.

• Gently retract the pipetting handle.

• To simplify this operation, a height

adjuster can be set by turning the

upper screw so that the pipetting head

may only be moved down to a position

slightly above the bottom.

Pipetting 384-well Microplates

PLATEMASTER is able to load 384-well plates with only four movements using the

384-well plate adapter.

STEP 1:

• Place a 384-well microplate on the 384-well plate adapter and make sure that it

is located at the first step by checking the position on the blue wheel.

• Aspirate the solution.

• Move the pipetting head over the microplate and dispense the first 96 wells.

STEP 2:

• Move the microplate on to the second position of the 384-well plate adapter

using the blue wheel.

• Aspirate the solution. Move the pipetting head over the microplate and

dispense the next 96 wells.

OPERATION

10 OPERATION | PLATEMASTER®

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