Endress+Hauser KRFB Manual de usuario
Products Solutions Services
Operating Instructions
Raman flow assembly
KRFB, KRFC
BA02296C/66/EN/01.23
70209490
Operating Instructions Raman flow assembly
2 Endress+Hauser
Raman flow assembly Operating Instructions
Endress+Hauser 3
Table of Contents
1 About this document ........................... 4
1.1 Warnings ................................................................ 4
1.2 Symbols on the device ........................................... 4
1.3 U.S. export compliance .......................................... 4
1.4 Glossary ................................................................... 5
2 Basic safety instructions ..................... 6
2.1 Personnel requirements ........................................ 6
2.2 Designated use ....................................................... 6
2.3 Workplace safety ................................................... 7
2.4 Operational safety .................................................. 7
2.5 Service safety .......................................................... 7
2.6 Important safeguards ............................................ 7
2.7 Product safety ......................................................... 7
3 Product description ............................. 8
3.1 Micro flow bench .................................................... 8
3.2 Micro flow cell ........................................................ 9
4 Incoming product acceptance and
product identification ....................... 10
4.1 Incoming acceptance .......................................... 10
4.2 Product identification ......................................... 10
4.3 Scope of delivery.................................................. 10
5 Installation ........................................ 11
5.1 Installing the micro flow bench ......................... 11
5.2 Inspecting the micro flow cell............................. 12
5.3 Installing tubing sets to the micro flow cell ...... 12
5.4 Installing the micro flow cell .............................. 13
6 Commissioning .................................. 14
6.1 Receipt of product ................................................ 14
6.2 Calibration and verification ................................ 14
7 Operation ........................................... 15
7.1 Micro flow cell ...................................................... 15
7.2 Storing the micro flow cell .................................. 15
7.3 Storing the micro flow bench ............................. 15
8 Diagnostics and troubleshooting ..... 17
9 Maintenance ...................................... 19
9.1 Cleaning the micro flow cell window ................. 19
10 Repair ................................................. 20
10.1 Repairing the Raman flow assembly ................. 20
10.2 User serviceable parts ......................................... 20
11 Technical data ................................... 21
11.1 Micro flow bench ................................................. 21
11.2 Micro flow cell ...................................................... 22
12 Supplementary documentation ....... 23
13 Index ................................................... 24
Operating Instructions Raman flow assembly
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1 About this document
This manual provides information about the flow cell apparatus used in conjunction with the Endress+Hauser
Rxn-10 Raman spectroscopic probe. This includes the wetted flow cell and the flow bench optical interface.
Refer to the Rxn-10 Raman spectroscopic probe Operating Instructions for specific probe-related information.
1.1 Warnings
Structure of Information Meaning
Causes (/consequences)
Consequences of non-compliance (if
applicable)
‣ Corrective action
This symbol alerts you to a dangerous situation. Failure to avoid the
dangerous situation can result in a fatal or serious injury.
Causes (/consequences)
Consequences of non-compliance (if
applicable)
‣ Corrective action
This symbol alerts you to a dangerous situation. Failure to avoid this
situation can result in minor or more serious injuries.
Cause/situation
Consequences of non-compliance (if
applicable)
‣ Action/note
This symbol alerts you to situations which may result in damage to
property.
Table 1. Warnings
1.2 Symbols on the device
Symbol Description
The Laser Radiation symbol is used to alert the user to the danger of exposure to hazardous visible laser
radiation when using the system.
The High Voltage symbol that alerts people to the presence of electric potential large enough to cause
injury or damage. In certain industries, high voltage refers to voltage above a certain threshold.
Equipment and conductors that carry high voltage warrant special safety requirements and procedures.
The WEEE symbol indicates that the product should not be discarded as unsorted waste but must be sent
to separate collection facilities for recovery and recycling.
Table 2. Symbols
1.3 U.S. export compliance
The policy of Endress+Hauser is strict compliance with U.S. export control laws as detailed in the website of the
Bureau of Industry and Security at the U.S. Department of Commerce.
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1.4 Glossary
Term Description
ANSI American National Standards Institute
API active pharmaceutical ingredient
ATEX atmosphére explosible
°C Celsius
cm centimeter
°F Fahrenheit
CRS calibration reference standard
in. inches
IO immersion optic
kg kilogram
LED light emitting diode
m meter
μin microinches
μm micrometer
mm millimeter
mW milliwatt
NCO non-contact optic
nm nanometer
oct/min octaves per minute
PD process development
psi pounds per square inch
WEEE Waste Electrical and Electronic Equipment
Table 3. Glossary
Operating Instructions Raman flow assembly
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2 Basic safety instructions
The safety information in this section is specific to the flow cell that is compatible with the Raman Rxn-10
spectroscopic probe from Endress+Hauser. Refer to the Rxn-10 Raman spectroscopic probe Operating Instructions for
additional information related to probe and laser safety.
2.1 Personnel requirements
• Installation, commissioning, operation, and maintenance of the probe/optics may be carried out only by
specially trained technical personnel.
• Technical personnel must be authorized by the plant operator to carry out the specified activities.
• Technical personnel must have read and understood these Operating Instructions and must follow the
instructions contained herein.
• The facility must designate a laser safety officer who ensures staff are trained on all Class 3B laser operating
and safety procedures.
• Faults at the measuring point may only be rectified by authorized trained personnel. Repairs not described in
this document must be carried out only directly at the manufacturer's site or by the service organization.
2.2 Designated use
The Raman micro flow cell and flow bench are designed for flow rates which are most applicable to product and
process development and are optimized for biological samples. The micro flow cell is designed to connect to a flow
path creating an enclosed, wetted interface which is separate from the flow bench. The flow bench, which is
compatible with the Rxn-10, allows for the secure laser-sample interface as well as calibration and verification of
the apparatus. The flow bench is required for use of the micro flow cell.
Recommended applications for the optics include:
Apparatus Fields of application
Flow cell Perfusion stream
Material recirculation loop
Tangential flow filtration
Eluate monitoring
Monomer-aggregate differentiation
Table 4. Fields of application
The above table lists common applications for the flow cell apparatus. There are other possible fields of application,
however, use of the device for any purpose outside of the fields of application described here poses a threat to the
safety of people and of the entire measuring system, and invalidates any warranty.
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2.3 Workplace safety
As the user, you are responsible for complying with the following safety conditions:
• Installation guidelines
• Local standards and regulations for electromagnetic compatibility
2.4 Operational safety
Before commissioning the entire measuring point:
• Verify that all connections are correct.
• Ensure that electro-optical cables are undamaged.
• Ensure fluid level and flow parameters are compatible with configured micro flow cell.
• Do not operate damaged products, and protect them against unintentional operation.
• Label damaged products as defective.
During operation:
• If faults cannot be rectified, products must be taken out of service and protected against unintentional
operation.
• When working with laser devices, always follow all local laser safety protocols which may include the use of
personal protective equipment and limiting device access to authorized users.
2.5 Service safety
Follow your company’s safety instructions when removing a process probe or optic from the process interface for
service. Always wear proper protective equipment when servicing the equipment.
2.6 Important safeguards
• Do not use the system for anything other than its intended use.
• Do not disconnect the Rxn-10 probe while the system is collecting Raman data.
• Do not remove the flow cell from the flow bench while the system is in use.
• Do not look directly into the laser beam.
• Do not point the laser at a mirrored or shiny surface, or a surface that can cause diffuse reflections. The
reflected beam is as harmful as the direct beam.
• When not in use, close the shutter on the Rxn-10 probe. If an optic cap is available, place it on the unused optic.
• Always use a laser beam block to avoid inadvertent scatter of laser radiation.
2.7 Product safety
The product is designed to meet all current safety requirements, and has been tested and shipped from the factory
in a safe operating condition. The relevant regulations and international standards have been observed. Devices
connected to an analyzer must comply with the applicable analyzer safety standards.
Operating Instructions Raman flow assembly
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3 Product description
The Raman flow cell and associated flow bench, powered by Kaiser Raman technology, enable enhanced signal
generation while monitoring material in a flow path. The system is an optimized non-contact apparatus that
leverages the internal design characteristics of the flow cell to enhance the total signal-to-noise ratio for faster
sampling times and lower limits of detection. Refer to the sections below for descriptions of the parts and their
functions.
3.1 Micro flow bench
The micro flow bench is configured and factory-tuned for the unique flow cell and application space needs, where
the fluid-path thickness and the refractive index of the sample are known or well approximated. For optimal
performance, it is recommended that the sample refractive index at the laser-wavelength be characterized so that
proper factory-setting can be achieved. The fluid-path thickness is defined by the flow cell configuration. It is
recommended that the micro flow bench and micro flow cell be configured together to ensure optimal performance
in the field. The micro flow bench has been optimized for use in the near infrared (NIR) region (785 nm).
The micro flow bench comes standard with a light shield to safely acquire the Raman spectra while eliminating
noise from the environment. In addition, a beam dump is available which safely blocks the amplifier to collect
spectra in back-scatter mode only. This is useful for running experiments that do not require the signal
enhancement.
The micro flow bench comes standard with an amplifier to enhance the Raman signal per unit-time.
3.1.1 Micro flow bench options
The micro flow bench provides application flexibility, and can be configured to the unique and demanding needs of a
variety of flowed-sample conditions. The micro flow bench is currently available in an Rxn-10 compatible
configuration which is factory tuned for the specific application needs. In most cases aqueous environments are an
acceptable approximation; however, extension beyond these spaces is available.
If a unique mounting solution is required, the micro flow bench can be configured with OEM-specific hardware to
directly integrate into the unique environment.
Raman flow assembly Operating Instructions
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3.2 Micro flow cell
The micro flow cell has been designed to produce laminar flow within a wide range of volumetric flowrates,
pressures, tubing diameters, and fitting options. Micro flow cells are ASME BPE SF4 Bio-Compatible and can be
cleaned and sterilized for re-use, or can be discarded after each use similar to sleeved immersion optics from
Endress+Hauser. Additionally, micro flow cells are configurable with a variety of fluid path thicknesses if the
standard 2.5 mm option is not sufficient.
3.2.1 Micro flow cell options
The micro flow bench and micro flow cell system has been designed to ensure maximum transferability between
cells and benches, allowing micro flow cells to be replaced on the flow bench with minimal impact to the resulting
spectrum.
3.2.2 Micro flow bench and flow cell data collection zone
The micro flow bench and flow cell are configured together to maximize the performance of the system. The
amplifier enhances the signal by collecting both forward and backward propagating scatter. The data collection zone
has been designed for optimal use in clear samples. If collection in opaque samples is required, contact Service
(Repairing the Raman flow assembly) → .
Operating Instructions Raman flow assembly
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4 Incoming product acceptance and product identification
4.1 Incoming acceptance
• Verify that the packaging is undamaged. Notify the supplier of any damage to the packaging. Keep the damaged
packaging until the issue has been resolved.
• Verify that the contents are undamaged. Notify the supplier of any damage to the delivery contents. Keep the
damaged goods until the issue has been resolved.
• Check that the delivery is complete and nothing is missing. Compare the shipping documents with your order.
• Pack the product for storage and transportation in such a way that it is protected against impact and moisture.
The original packaging offers the best protection. Make sure to comply with the permitted ambient conditions.
• If you have any questions, please contact your supplier or your local sales center.
Incorrect transportation can damage the micro flow cell and micro flow bench.
4.2 Product identification
4.2.1 Label
At a minimum, the optics are labeled with the following information:
• Manufacturer information
• Part number
• Serial number
• Wavelength
• KMAT (product configuration code)
Compare the information on the label/tag with the order.
4.2.2 Manufacturer address
Endress+Hauser
371 Parkland Plaza
Ann Arbor, MI 48103 USA
4.3 Scope of delivery
The scope of delivery comprises:
• Raman micro flow bench
• Raman micro flow cell
• Raman flow assembly Operating Instructions
• Local declarations of conformity, if applicable
• Material certificates, if applicable
If you have any questions, please contact your supplier or local sales center.
Este manual sirve para los siguientes modelos
1
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