BestScope BS-2010E Manual de usuario

Bestscope International Limited

Biological Microscope

BS-2010E

User Manual

This manual is for users of biological microscope BS-2010E. To ensure the safety, obtain optimum

performance and to familiarize yourself fully with the use of this microscope, it is recommended

strongly that you study this manual thoroughly before operating the microscope. Retain this

instruction manual in an easily accessible place near the microscope for further reference

BestScope International Limited

Eyepiece

Objective

Monocular Head

Coarse Focusing

Knob

Fine Focusing

Knob

Brightness

Adjustment

Knob

Main

Switch

Stage

Condenser

Collector

Bestscope International Limited

1 Appliance

BS-2010E biological microscope is widely used in medical and hygienic establishments for

conventional microscopic examination, general biologic, pathologic and bacteriological studies,

clinical investigations and classroom demonstrations. Designed in a very modern way what can

take convenient and safe to your operations.

2 Specification

2.1 Mechanical tube length：160mm

2.2 Objective：

Magnification Numerical

Aperture

（NA）

Focus

Distance

(mm)

Working

Distanc

(mm)

Remark

4X 0.10 31.05 18

10X 0.25 17.13 7

Eyepiece

Objective

Stage

Condenser

Collector

Sliding

Binocular

Head

Coarse

Focusing

Knob

Fine

Focusing

Knob

Brightness

Adjustment

Knob

Main

Switch

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40X 0.65 4.65 0.53

100X 1.25 2.906 0.13

2.3 Eyepiece

TYPE Magnificatio

Focus

Distance(mm) Field of view (mm)

Plan Eyepiece 10X 25 Ø18

2.4Total Magnification

Eyepiece 10X 10X 10X 10X

Objective 4X 10X 40X 100X

Total 40X 100X 400X 1000X

2.5 Conjugate Distance: 195mm

2.6 Stage：

BS-2010 D、E: Double Layers Mechanical Stage 116mmX125mm

BS-2010 A、B: Attachable Mechanical Stage 110mmX120mm

BS-2010 C: Plain Stage with Slide Clips 110mmX120mm

2.7 Coarse Focusing range：10mm

2.8 Condenser：

BS-2010 D、E:Abbe NA=1.2with Iris Diaphragm,Rack and Pinion Adjustment

BS-2010 A : Single Lens NA 0.65 with Disc Diaphragm

BS-2010 B : Single Lens NA 0.65 with Iris Diaphragm

BS-2010 C : Abbe NA=1.2with Iris Diaphragm,SpiralAdjustment

2.9 Diameter of Color Filter：Ø32mm

2.11 Illumination ：

BS-2010 A、B、C、D:Incandescent Lamp Illumination 15W,

Brightness Adjuseable

BS-2010 E: LED Lamp Illumination 1W, Brightness Adjuseable

3 Outfits

Component

Name

Specification BS-2010A BS-2010B BS-2010C BS-2010D BS-2010E

Monocular Head,

inclined at 45° ●●●●

○

Viewing

Head

Sliding Binocular

Head,Inclined at ○○○○

●

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45°

Wide Field

Eyepiece

WF10X

●●●●●

Eyepiece

WF5X,P16X ○○○○○

Eyepiece

Eyepiece WF10X

with Pointer ○○○○○

4X,10X ,40X(S) ●●●●●

20X,60X ○○○○○

Objective

(Achromatic)

100X ○○○○●

Single Lens NA

0.65 with Disc

Diaphragm

●○○○○

Single Lens NA

0.65 with Iris

Diaphragm

○●○○○

Abbe NA=1.2with

Iris

Diaphragm,Spiral

Adjustment

○○●○○

Condenser

Abbe NA=1.2with

Iris

Diaphragm,Rack

and Pinion

Adjustment

○○○●●

Triple Nosepiece ●●●●○

Nosepiece

Quadruple

Nosepiece ○○○○●

Plain Stage with

Slide Clips 110×

120mm

●●○○○

Attachable

Mechanical Stage

110

120mm / 60

30mm

○○●○○

Stage

Double Layers

Mechanical

Stage

125

115mm /

20mm

○○○●●

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Incandescent Lamp

15W

Brightness

Adjustment

●●●●○

Illumination

LED Lamp 1W

Brightness

Adjustment

○○○○●

Mirror ○○○○○

Focusingg

Systerm

Caaxial Coarse and

Fine

Adjustment,Movin

g Range 10mm

●●●●●

Green ○○●●●Filter

Blue ○○●●●

Note: ●Standard Outfit, ○Optional

4 Configuration

a) Eyepiece, Objective and Nosepiece

The monocular microscope has wide field eyepiece 10X and achromatic objective

4X, 10X, 40X and 100X. The nosepiece is small structured, and located stably and

exactly. It has a large and sharp image, with 360º rotatable monocular or sliding

binocular head.

b) Bend arm and Coarse and fine focusing knob

The curved arm use C-shaped ,has sprightly lines.Also, with upper focus

stop.the coarse focusing adjustment adopts dovetail guide which make focusing

adjustment smooth and comfortable.

c) Base：

The base is designed to the instrument, which having a streamlined shaped

circular,and it harmonized the aim’s shape slinky，Electrical components and

lamp built inside it as the illuminator.

d) Stage：

Fixed stage or moveable stage

e) Illumination

The system consists of illuminator which contains collector / mirror, iris aperture

diaphragm and condenser. And the condenser can be lift up and down.

5 Assembly

a) Working environment requirement：

1) Room temperature: 0-40℃.

Maximum comparatively humidity: 85%.

2）High temperature will result in mildew, dew and even ruinous instrument.

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3) Avoid from the dust room. When it is not in use, please cover the

microscope with dust cover.

4）Please place the microscope in a stable situation without any

surging.

b) Check the Input voltage: be sure the power supply voltage is accordant with the

nominal input voltage which is signed outside the microscope, or it will bring a serious

damage to the microscope.

c) Lamp

1. The lamp has been well adjusted and checked before the microscope

leaves factory.

2 .The lamp housing parts become extremely hot during operation. Take care

not to burn yourself.

3. Lamp replacement：The lamp has its standard service life. When it has

expired, a lamp replacement is necessary.set the main switch to off state,

disconnect the power cord from the wall outlet, then allow the old bulb to

cool before replacing the bulb with a new of the designated type .Open the

lamp holder on the bottom of microscope to do the replacement, the surface

of the new lamp bulb should be clean and free of fingerprint or dirt, which will

decrease the brightness or even explode the bulb .

Note: Must not missing the grounded screw

Donot touch the lamp`s glass part with your hands,when sitting shoud

take gloves or cover the lamp with protector. Fingerprint or dirt on the

surface of the lamp should be cleaned with the clean cloth with dipping

alcohol,which will decrease the brightness or even explode the bulb.

d) Eyepiece：

Please insert the eyepieces into the eyepiece tubes.(There is small screw in

the eyepiece ,which can make the eyepiece can not be pull out arbitrarily

when be screwed down.

e) Objective：

Lower the stage to its lower limit, then screw down the objectives into the

nosepiece (from low to high).

f) Condenser：

he condenser has been adjusted well before delivery.

g) Collector：

The collector has been adjusted well before delivery. Frame of the

collector`s pedestal is screw thread can taken by contrarotate

Note：It cannot be taken down when the outfit is LED.

h) Color filter：

Lay the filter into the iris diaphragm bracket under the condenser if

necessary.

6 Operation

1、Insert the eyepiece into the inclined eyepiece tube, then rotate the objectives

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in sequence, according to their magnifications, into the threaded holes of the

nosepiece. Place the specimen in the center of the field of view, first use low

power objective to find the specimen image and then observe it with high

power objective, at the same time, adjust fine focusing knob until the image is

clear. When using 100X oil-immersion objective, the space between the front

of objective and specimen surface should be full of cedar oil. Drops of cedar

oil must be strictly free from air bubble, and the objective should be cleaned

immediately after using, otherwise it may solidify and make cleaning

difficult.

2、In order to get a sharp and clear image, you must adjust the illumination

system. When using objectives of different magnification, please adjust the

iris diaphragm of the condenser to bring its numerical aperture and objective’s

numerical aperture into coincidence. Generally speaking, the rate of objective

increase，the location of the condenser need to move up. Aperture is design for

adjust NA and not for adjust brightness. Usually, aperture is open to 70~80% of

the objective’s exit pupil, until can see clearly image in the view.

3、When to watch the same type of specimens, can adjust limit screw to proper

position and lock it up with nut. Then directly make coarse focusing knob up to the

position where the first speciment can focusing clearly. That can simplify the

operation and reduce the working intensity.

Note: The same type of specimen refer to the thickness of the slice 、the coverslip

and the carry slip are uniform.

7 Maintence and storage

1、When you open the carton, please be careful not to make the lens dropping

2、All the lens has been adjusted by manufacturer already, please do not

disassemble by yourself.

3、Nosepiece, coarse and fine focus is installed precisely, please do not

disassemble by yourself.

4、You should make the instrument clean, and often wipe the dusts.

5、Place the instrument in a shady, cool and dry place, when finishing the

operation, always use the dust cover for protection.

8 Troubleshooting Guide

If problems occur during use, please review the following list and take remedial

action as needed. If you cannot solve the problem after checking the entire list,

please contact the service department for assistance.

1、Optical Part

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Problem Cause Solution

The nosepiece is not in he located

position(objective is

not in the center of

the optical path)

Turn to the right

position(Turn objective

to the optical light

center)）

The image of the filament is not

in the center Center it

the edge of the field of

view has shadow or the

brightnessis uneven

The surface of the lens is moldy

or has contaminant (

including condenser,

objective, eyepiece and

collector)

Clean it up

There are stains on the lens

(include

condenser,objective, eyepiece and

collector)

Clean it up

There are stains on the specimen Clean it up

Find dust and stain in

the field of view

The position of the condenser is too

low Loosen the

condenser’s locking bolt

adjust the

condenser to the

right position, then

screw down.

There is no coverslip on the

specimen add coverslip

The coverslip is too thick or too thin Use the standard

coverslip

The specimen is placed inversely Reverse it back

There was oil on the dry

objective(easily happened in 40X

objective)

Clean it up

the image is

defocus(low

resolution/contrast) There are stains on the lens

（including condenser, objective,

eyepiece and collector）

Clean it up

The oil objective didn’t immerse

oil Use immerse oil

There was bubbles in the oil Eliminate the bubbles

Use a unsuitable oil Change to the

specified one

The size of the aperture

diaphragm is too big Minify it

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There are stains on the incident

lens of the binocular tube Clean it up

The size of the aperture

diaphragm is too small Open it up

The position of the condenser is

too low Adjust the position

The condenser is not in the

center of the field of view\the

condenser inclines

Install the condenser

again and adjust the

center carefully by the

centering bolt

The nosepiece is not in the right

position Turning it until it reach

the “clicked” position

One side of the image

is dark

The specimen is floating Fix it

The specimen slips on the stage Fix itThe image shifts

during focusing The nosepiece is not in the right

position Turn it to the

“ clicked ”position

The image is a little

yellow Not use the blue color filter Use the blue filter

The size of the aperture

diaphragm is too small Adjust again

The position of the condenser is

too low Adjust the position

The brightness is not

enough

There are stains on the lens

（include condenser, objective,

eyepiece and collector）

Clean it up

2、Mechanical Part

Problem Cause Solution

The image can not

focus when using

high magnification

objective

The specimen is placed

inversely

The coverslip is too thick

Turn inversely

Use the standard coverslip

（0.17 mm）

The objective and the

specimen touch

when change the low

magnification to the

higher magnification

The specimen is placed

inversely

The coverslip is too thick

Turn inversely

Use the standard coverslip

（0.17 mm）

The specimen is

not easy to move The specimen holder is not

fixed Fix it

The eyes is too tired No diopter adjustment Adjust the diopter correctly

The brightness is not

suitable Adjust the voltage of the

lamp

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3、Electrical Part

Problem Cause Solution

No power Check the connection of the

power cord

The bulb is not inserted Insert it correctly

The lamp can’t light

when turning on the

switch The bulb burns out Replace it

The lamp burns out

suddenly Use an unstandard lamp

The voltage is too high

Use the specified lamp to

replace, if the problem is not

solved, contact with the

service department

Use a substandard lamp Use the specified lampThe brightness is not

enough The voltage is too low Add the voltage

The bulb flickers or

the brightness is

vertiginous

The bulb is going to burn

out Replace it

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