Agilent Technologies InfinityLab LC Series Manual de usuario

Agilent Technologies

Agilent InfinityLab LC Series

1260 Infinity II SFC System

User Manual

Notices

2017

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Manual Part Number

G4301-90104

Edition

02/2017

Printed in Germany

Agilent Technologies

Hewlett-Packard-Strasse 8

76337 Waldbronn

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In This Guide...

This manual covers the Agilent InfinityLab LC Series 1260 Infinity II SFC

System.

1 Introduction to Supercritical Fluid Chromatography (SFC)

This chapter provides an overview of the history, theory and benefits of SFC.

2 Site Requirements and Specifications

This chapter provides information on environmental requirements, physical

and performance specifications only for the Agilent 1260 Infinity II SFC

System.

3 Installing the Agilent 1260 Infinity II SFC System

This chapter provides an overview of the installation and setup of the

hardware and software

4 Configuring the System

How to configure the Agilent 1260 Infinity II SFC System and Agilent 1260

Infinity II SFC Control Module in OpenLAB CDS.

5 Using the Agilent 1260 Infinity II SFC Control Module

This chapter provides information and hints on the use of the SFC System.

6 Maintenance and Repair

In this chapter only the SFC specific procedures are described. For procedures

similar to the Agilent module procedures, please refer to the single module

manuals (G4782A, G4767A, G7116A, G7115/65A)

7PartsforMaintenance

This chapter provides information on parts for maintenance and repair.

In This Guide...

8 Identifying Cables

This chapter provides information on cables used with the modules.

9 Appendix

This chapter provides addition information on safety, legal and web.

Contents

1 Introduction to Supercritical Fluid Chromatography (SFC) 7

History of SFC 8

Theory of SFC 9

Benefits of SFC 10

Common flow path overview for packed column SFC instrumentation 11

The Agilent 1260 Infinity II SFC System 12

2 Site Requirements and Specifications 21

Site Requirements 22

Specifications 26

3 Installing the Agilent 1260 Infinity II SFC System 31

Hardware Installation 32

4 Configuring the System 55

Configuring the SFC system in OpenLAB CDS 56

Setting up the Method 60

Status 62

Control 63

5 Using the Agilent 1260 Infinity II SFC Control Module 65

Powering up the Module 66

Power-up Sequence and Operational Control States 67

Operational Control States 68

Controlling the Agilent 1260 Infinity II SFC Control Module through the Agilent

OpenLAB CDS 71

Running a method on the SFC system 73

Shutting Down the SFC System 75

Contents

6 Maintenance and Repair 77

Inspection and Preventative Maintenance Intervals 78

General Maintenance procedures 80

7PartsforMaintenance 91

Agilent 1260 Infinity II SFC Control Module Parts 92

Agilent 1260 Infinity II SFC DAD Parts 94

8 Identifying Cables 95

Cable Overview 96

Analog Cables 98

Remote Cables 100

CAN/LAN Cables 104

RS-232 Cables 105

USB 106

9 Appendix 107

General Safety Information 108

The Waste Electrical and Electronic Equipment (WEEE) Directive

(2002-96-EC) 112

Radio Interference 113

Sound Emission 114

Solvent Information 115

Agilent Technologies on Internet 117

Agilent Technologies

Introduction to Supercritical Fluid

Chromatography (SFC)

History of SFC 8

Theory of SFC 9

Benefits of SFC 10

Common flow path overview for packed column SFC

instrumentation 11

The Agilent 1260 Infinity II SFC System 12

The Agilent 1260 Infinity II SFC Control Module (G4301A) 13

The Agilent 1260 Infinity II SFC Binary Pump (G4782A) 14

The Agilent 1260 Infinity II SFC Multisampler (G4767A) 15

The Agilent 1260 Infinity II Multicolumn Thermostat (G7116A) 16

The Agilent 1260 Infinity II Diode Array Detector WR (G7115A) and

Agilent 1260 Infinity II Multi Wavelength Detector (G7165A) 18

Applications 18

Columns 19

This chapter provides an overview of the history, theory and benefits of SFC.

Introduction to Supercritical Fluid Chromatography (SFC)

History of SFC

Supercritical fluid chromatography (SFC) was first introduced by Klesper et

al. in 1962 (Klesper, E.; Corwin, A. H.; Turner, D. A. J. Org. Chem. 1962,

27,700.) for the separation and analysis of a porphyrin mixture using open

tubular SFC. The first commercial instruments using packed columns were

available from Hewlett-Packard (HP) in 1982. Since then, several vendors have

developed and commercialized packed column SFC instrumentation for

analytical as well as for preparative separation. SFC is widely accepted for the

separation of chiral compounds and increased user interest has been observed

for a wide spectrum of small to medium sized molecules due to the analysis

speed achieved and the low solvent consumption.

The latest introduction of analytical SFC instrumentation, the Agilent 1260

Infinity II SFC Control Module coupled to an Agilent 1260 Infinity II Binary

LC system optimized for SFC.

Introduction to Supercritical Fluid Chromatography (SFC)

Theory of SFC

Figure 1 State of a solvent

The superior separation properties achieved by SFC can be explained best by

the thermodynamics of liquids and gases (see the phase diagram in Figure 1

on page 9). Above a critical pressure, liquids can no longer enter the gaseous

state; similarly, above a critical temperature, gases cannot be converted to

liquids. Above both the critical pressure and temperature (characterized by

the critical point), solvents are in the supercritical state. Under these

conditions, the mobile phases exhibit gaseous as well as liquid-like properties.

The major advantages of this state related to chromatography are improved

diffusion characteristics and mass transfer and low viscosity, which result

in high separation efficiency and fast separation capability.

Introduction to Supercritical Fluid Chromatography (SFC)

Benefits of SFC

SFC is widely accepted for the analysis and separation of chiral compounds. In

addition, it gains increasing acceptance as a complementary liquid-based

separation technique to HPLC for high-throughput and high-resolution

analysis of complex mixtures. This is due to the thermodynamic properties of

supercritical fluids, which can be exploited for high throughput and high

efficiency. In addition, the mild thermal conditions also allow for the analysis

of thermally labile compounds. Typically, analysis times and column

re-equilibration are decreased by a factor of 3–5 compared to standard HPLC.

With the increasing costs of organic solvents and the environmental

awareness to minimize toxic waste, production SFC is increasingly accepted

as the “green alternative” to normal phase or reversed phase chromatography,

gaining popularity in method development and UV- and MS-based separation

and purification. A variety of parameters, such as stationary phase selection,

mobile phase composition, modifier type and concentration, column

temperature and system pressure, can be easily manipulated to fulfill

separation requirements by influencing, optimizing and exploiting selectivity

in SFC.

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