UGO BASILE 37240 Manual de usuario
SAFETY
CONSIDERATIONS
Although this instrument has
been designed with international
safety standards, it contains
information, cautions and warnings
which must be followed to ensure
safe operation and to retain the
instrument in safe conditions.
Service and adjustments should
be carried out by qualied
personnel, authorized by Ugo Basile
organization.
Any adjustment, maintenance and
repair of the powered instrument
should be avoided as much as
possible and, when inevitable,
should be carried out by a skilled
person who is aware of the hazard
involved.
Capacitors inside the instrument
may still be charged even if the
instrument has been disconnected
from its source of supply.
Table of Contents
1. Product features and general information 5
2. What’s in the box 6
3. General 9
3.1. Principle of operation ............................................................................ 9
4. Intrument description 10
4.1. The Electronic Unit 37240-001............................................................ 10
4.2. The water cell........................................................................................ 10
5. Installation 11
5.1. Unpacking & preliminary check ......................................................... 11
5.2. Notes on the User Guide..................................................................... 11
5.3. Before Applying Power........................................................................ 11
5.4. Intended Use ........................................................................................ 12
5.5. Additional Safety Consideration......................................................... 12
5.6. Connections .......................................................................................... 12
6. Operation 14
6.1. Setting-Up the Plethysmometer......................................................... 14
6.1.1. Preparing the dipping solution .................................................................... 14
6.1.2. Main Menu........................................................................................................ 15
6.1.3. Utilities Menu ................................................................................................... 16
6.1.4. Device setup menu ......................................................................................... 18
6.2. Calibration............................................................................................. 20
6.2.1. How to calibrate............................................................................................... 20
6.3. Measurement ....................................................................................... 21
6.3.1. Manual measurement .................................................................................... 22
6.3.2. Automatic measurement............................................................................... 22
6.4. Restoring Liquid Level ......................................................................... 23
6.5. Results ................................................................................................... 24
6.5.1. Results and Experiment menus ................................................................... 24
6.5.2. USB results export........................................................................................... 25
6.5.3. Ethernet (LAN) data load and retrieve ........................................................ 25
6.6. TTL Connections ................................................................................... 28
7. Maintenance 29
7.1. Electrical ................................................................................................ 29
7.2. Cell.......................................................................................................... 29
7.3. Transducer ............................................................................................ 29
7.4. Long Inactivity ...................................................................................... 30
7.5. Customer Support................................................................................ 30
8. Specification 31
9. Bibliografy 32
10. Related products 33
Product features and general information 5
This original device by Ugo Basile was designed to measure paw volume changes
(swelling) in rodents.
With almost 4,000 peer-reviewed citations, Ugo Basile Plethysmometer is the gold
standard for paw swelling tests.
Main features include:
Touch screen display, friendly interface, USB data retrieval and experiment data
loading capabilities, LAN connection, auto-measure function and Ugo Basile
family feeling design.
Displays the exact paw volume on the touch screen display, read-out with
resolution of 0.01ml. Small differences are detected by an high precision
self-made level transducer.
Foot pedal that allows hands-free operation, to save measured value and to
enable auto-measure function.
Easy zeroing before making new measurements.
Measuring paw tubes available in different sizes for different animals paws.
Web-based LAN connection for data retrieving and experiment data loading
(password protected).
USB pen drive for data retrieving and experiment data loading.
1. Product features and general information
Back to Contents
What’s in the box
6
2. What’s in the box
Plethysmometer standard kit -SKU: 37240
SKU Q.ty Description
37240-001 1 Plethysmometer electronic unit
37240-210 1 Plethysmometer cell stand
37252 1 Standard water cell with Mouse paw tube (diam.13
mm) and Rat paw tube (diam. 18 mm) with water level
transducer
3740-154 1 Water tank
7155 1 Set of 6 calibration probes (0.1, 0.2, 0.5, 1, 2, 4 ml)
7160 1 Wetting Compound, 100 ml bottle
M-TF 050-F 1 2 mt. of Polyurethane tube for connection: cell to water
tank & cell to drain
E-AU 101 1 USB Pen Drive including instruction manual (with CE certi-
cation) and X-Pad software
37215-303 1 Pedal Switch
Plethysmometer kit with 25mm Rat paw tube cell -SKU: 37240-25
SKU Q.ty Description
37240-001 1 Plethysmometer electronic unit
37240-210 1 Plethysmometer cell stand
37257 1 Special water cell with Rat paw tube (diam. 25 mm) with
water level transducer
3740-154 1 Water tank
7155 1 Set of 6 calibration probes (0.1, 0.2, 0.5, 1, 2, 4 ml)
7160 1 Wetting Compound, 100 ml bottle
M-TF 050-F 1 2 mt. of Polyurethane tube for connection: cell to water
tank & cell to drain
E-AU 101 1 USB Pen Drive including instruction manual (with CE certi-
cation) and X-Pad software
37215-303 1 Pedal Switch
Back to Contents
What’s in the box 7
Plethysmometer kit with 35mm Rat paw tube cell -SKU: 37240-35
SKU Q.ty Description
37240-001 1 Plethysmometer electronic unit
37240-210 1 Plethysmometer cell stand
37259 1 Special water cell with Rat paw tube (diam. 35 mm) with
water level transducer
3740-154 1 Water tank
7155 1 Set of 6 calibration probes (0.1, 0.2, 0.5, 1, 2, 4 ml)
7160 1 Wetting Compound, 100 ml bottle
M-TF 050-F 1 2 mt. of Polyurethane tube for connection: cell to water
tank & cell to drain
E-AU 101 1 USB Pen Drive including instruction manual (with CE certi-
cation) and X-Pad software
37215-303 1 Pedal Switch
Optional items ordering information
SKU Description
37252 Standard water cell with Mouse paw tube (diam. 13 mm) and Rat
paw tube (diam. 18 mm) with water level transducer
37252-S Standard water cell with Mouse paw tube (diam. 13 mm) and Rat
paw tube (diam. 18 mm) without water level transducer
37257 Special water cell with Rat paw tube (diam. 25 mm) with water
level transducer
37257-S Special water cell with Rat paw tube (diam. 25 mm) without
water level transducer
37259 Special water cell with Rat paw tube (diam. 35 mm) with water
level transducer
37259-S Special water cell with Rat paw tube (diam. 35 mm) without
water level transducer
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What’s in the box
8
Available spare parts
SKU Description
7182 18 mm tube for the rat paw
7186 13 mm tube for the rat paw
7187 25 mm tube for the rat paw
7189 35 mm tube for the rat paw
7153-L Water level transducer
E-BT 008 Battery type CR2032
E-FT010-1 2 X fuse T1.25A - 6X32 mm
Consumables
SKU Description
7160 Wetting Compound, 100 ml bottle
LAN connection default password is:
UgoBasile (CASE SENSITIVE)
Back to Contents
General 9
3. General
In research on rheumatoid arthritis, central development of oedema and
its modifications by pharmacological processes, the measurement of the
inflammatory processes in the paws of mice and rats has proven to be of great
value.
The Ugo Basile Plethysmometer is a volume meter, designed for accurate
measurements of the rodent paw swelling.
It consists of a liquid-filled cell into which the rodent paw is dipped. A transducer
of original design, which measures small differences in liquid level caused by
volume displacement, is connected to an electronic unit used to show, save and
recall the values.
3.1. Principle of operation
The measuring cell consists of two vertical interconnected Perspex tubes, the
larger of which is used to measure displacement. The water level in the smaller
tube, which contains the transducer, follows that of the large one and is therefore
proportional to the volume dipped in the large tube.
We have developed an original transducer which measures the conductance
between two vertical wire electrodes.
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Intrument description
10
4. Intrument description
4.1. The Electronic Unit 37240-001
The Plethysmometer Electronic Unit is a microprocessor-controlled unit with
touch screen and user-friendly graphical interface which has the capability to
store a great number of measurements; load and recall experiments and can
communicate to a PC through a LAN connection via web browser or via the
provided USB stick. (FAT formatted)
The experimental data are shown on the multifunction graphic display, in four
digits, with 0.01 ml resolution.
4.2. The water cell
The standard kit SKU: 37240 includes a standard water cell for Rat, diam. 18 mm,
provided with an extra paw tube diam. 13 mm for measuring the mouse paw
volume.
The Plethysmometer can be ordered with a different water cell configurations:
• SKU: 37240-25, including a 25mm diam. Water Cell
• SKU: 37240-35, including a 35mm diam. Water Cell
37240-25 & 37240-35 kit, includes all the Plethysmometer standard accessories
and only differs from the standard kit for the water cell capacity.
Other water cells are available as a separate part, see ugobasile.com web site for
more informations.
Water Cell with transducer, Frontal and lateral view
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