Thermo Scientific ERGOSTAR HM 200 Manual de usuario

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

Thermo Scientific

SECTION TRANSFER SYSTEM Microm STS

For ROTARY MICROTOMES / ERGOSTAR HM 200

387712 Issue 8

INSTRUCTION MANUAL

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

CERTIFICATION

Thermo Fisher Scientific Microm International GmbH certifies that this

instrument has been tested and checked carefully. Its technical data was

verified before shipment to be in accordance with the published

specifications.

The instrument complies with applicable international safety regulations.

WARRANTY

This product is warranted against defects in material and workmanship for a

period of 1 year. Parts which prove to be defective during the warranty

period will be repaired or replaced free of charge by Thermo Fisher Scientific

Microm International GmbH. No other warranty is expressed or implied.

Unauthorized modification or repair by third party persons will void the

warranty.

The warranty will expire in case of improper or wrong use of the instrument

and in case the warning and precautionary messages are not observed.

Thermo Fisher Scientific Microm International GmbH is not liable for any

occurring damage.

Errors and omissions excepted. Subject to amendment and improvement

without further notice.

This instruction manual will be supplied together with each instrument.

Further copies can be ordered at the nearest sales office by giving the serial

number of the instrument, the number of the instruction manual and the date

of issue.

This instruction manual is available in the following languages:

Cat. No.

German: 387711

English: 387712

French: 387713

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

INTENDED USE

Dear Customer,

Thank you for buying this Thermo Scientific instrument.

Before putting the instrument into operation, please read these

operating instructions carefully to familiarize yourself with its proper

operation and functions.

Only skilled or specially trained personnel must operate the

instrument, i.e. clamping the specimen, trimming, sectioning and

taking off the sections from the instrument. The listed and marked

safety measures as well as the regulations and hygiene measures of

your respective lab must strictly be observed.

Ser. No. :..................................................

Please enter the serial number, which is placed on the type plate on

the rear side of the instrument. This way, questions and service can

be handled faster.

Instruction manual no. 387712

Issued on 02.10.2014

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

Intended use

Safety precautions

PART 1 INTRODUCTION

1-1 Description of the Section Transfer System STS

1-2 Technical data Section Transfer System

PART 2 OPERATING INSTRUCTIONS

2-1 Initial turn-on

2-2 Trim sectioning of specimens

2-3 Fine sectioning of specimens

2-4 Draining and cleaning the water bath and the pump reservoir

2-5 Cleaning the pump filter

2-6 Disposable blade carrier TE

2-7 Standard equipment

2-8 Additional equipment

PART 3 THEORY OF OPERATION OF THE SECTION TRANSFER SYSTEM

3-1 Water circulation

3-2 Cutting and detaching a section

PART 4 WORKING WITH THE SECTION TRANSFER SYSTEM

4-1 Preparing the Section Transfer System

4-2 Coarse feed and trimming function

4-3 Sectioning and picking up sections

4-4 Working with the STS in combination with the Cool-Cut

4-5 How to avoid malfunctions

PART 5 MAINTENANCE

PART 6 CLEANING AND CARE

6-1 Cleaning

6-2 Disposal of the instrument after final shutdown

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

SAFETY PRECAUTIONS

WARNING SIGNALS AND SYMBOLS

The installation and routine use of the STS is easy and safe if the instructions in this manual are being

observed.

However, the situations which might be risky for the personnel or equipment are specially marked in

this manual with the following symbols and messages.

Note:

Special instructions regarding operation of the instrument.

Caution:

Special precautionary measures to prevent damage to equipment. For a long lifetime of the

equipment, please observe these instructions carefully.

Danger:

Special warning messages to prevent harm to persons and/or serious damage to equipment.

For your own safety, please observe these instructions carefully.

Biohazard:

Warning of biological danger.

Separate taking back of electrical and electronic instruments in the countries of the

European Union:

This is to be applied in the countries of the European Union and other European countries

with a separate collecting system within the waste management.

This product, being an electro and/or electronic instrument, must be treated separately

within the waste management process (WEEE).

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

SAFETY PRECAUTIONS

A T T E N T I O N !

Please observe the following general precautions during operation of this instrument.

Failure to comply with these precautions violates safety standards and the intended

use of the instrument. Thermo Fisher Scientific Microm International GmbH is not

liable for misuse of the instruments and failure to comply with basic safety

requirements.

INSTRUMENT GROUNDING

To avoid injury from electrical current, the instrument must be connected with the safety ground. The

instrument is equipped with a three wire ground plug. The power outlet must be connected to the

safety ground and must meet the International Electro technical Commission (IEC) regulations.

CAUTION: MAINS VOLTAGE

Never remove instrument covers during operation. Component replacements as well as adjustments

must only be made by trained service personnel. Unplug the unit before removing or opening the

covers. Only original spare parts must be used for replacement work.

DANGER IN EXPLOSIVE ENVIRONMENT

The instrument must not be operated in the presence of flammable gases.

HAZARD OF RADIOACTIVE RADIATION

When working with radioactive specimens observe all applicable radiation safety procedures. When

working with radioactive contaminated material, appropriate safety and disinfection measures must be

carried out. According to the rules and regulations concerning the handling of radioactive

contaminated material of the respective laboratory, safety clothing (e.g. particle mask, gloves,

protective shoe covers) must be worn.

Radioactive contaminated waste must be disposed of according to the respective regulations.

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

HAZARD OF WATER

Set up and operate the separate control unit in a way that it is protected from water.

HAZARD OF BIOLOGICAL DANGER

Specimens used during the intended operation of the instrument might potentially be infectious. For

this reason, it is recommended to observe the general laboratory regulations concerning protection

against danger of infection.

Information on decontamination media, their use, dilution and effective range of application can be

read in the Laboratory Biosafety Manual : 1984 of the World Health Organization.

HAZARD OF MALFUNCTION

To avoid the hazard of malfunction of an instrument, it must only be operated in a controlled

electromagnetic environment. This means that transmitters such as mobile phones must not be

operated in their close vicinity.

DANGER WHEN USING OPERATING MATERIALS

Xylene and other solvents may be considered hazardous. For this reason, please observe the

stipulated safety measures to avoid contact with eyes and skin (e.g. wear protective gloves).

WASTE DISPOSAL

All debris, waste as well as infectious and radioactive contaminated material from operation must be

disposed of in accordance with the respective regulations of the lab. Disinfection and cleaning liquids

as well as section waste must be disposed of according to the respective regulations for special waste!

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

Fig. 1

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

1 INTRODUCTION

1-1 Description of the Section Transfer System STS

The system consists of a special disposable blade carrier TE with integrated transfer bridge, a heated

water bath as well as a control unit.

The Section Transfer System can be used along with all current rotary microtomes and the Ergostar

HM 200.

It takes a few minutes only to upgrade the microtome from the common paraffin sectioning technique

to the STS method.

The STS is used solely for tissue specimens embedded in paraffin. During the sectioning process with

the STS, sections glide on a water film via the disposable blade carrier TE and the transfer bridge and

are then transferred into the water bath.

Water circulation is guaranteed by a pump, the capacity of which can be adjusted.

The water temperature of the water bath can be adjusted between ambient temperature and +45°C.

Only use demineralized or distilled water!!

Due to the use of the STS damage to the tissue sections can be diminished by gliding on the water

flow. The sections are stretched during the cutting process due to the smooth water flow. This way,

extremely thin as well as high-quality sectioning results can be obtained.

The common manual transfer of the sections into a water bath is no longer necessary.

When the sections are generated, their quality can be checked immediately, i.e. when the ribbon or

the individual sections are stretched on the water flow of the disposable blade carrier TE. The second

quality check can be carried out in the water bath facilitated by the integrated section illumination.

The sections which are not used, e.g. from trimming paraffin blocks, can be guided directly into a

waste basket via the water flow on the disposable blade carrier TE.

The water bath, the waste container as well as the waste filter of the STS can easily be removed and

cleaned.

The integrated water container for the pump circulation is freely accessible and allows for easy

cleaning.

Thermo Scientific Section Transfer System Microm STS

Microm International GmbH

Part of Thermo Fisher Scientific

Im Steingrund 4 - 6

63303 Dreieich / Germany

387 712 - English

1-2 TECHNICAL DATA SECTION TRANSFER SYSTEM

Heated water bath: +45°C

Volume of heated water bath: 800 ml

Volume of pump reservoir: 600 ml

Required liquid: demineralized or distilled water

Flow rate: 0 ml/min. - 500 ml/min.

Operating conditions: +10°C to + 35°C (at max. 60% rel. humidity of the air)

altitude up to 2000 M.S.L.

for indoor use only

Pollution degree: 2

Overvoltage category: II

Acoustic pressure: 40 dB(A)

Power requirements: 220 - 240 V, 50/60 Hz, 0,7 A

100 - 120 V, 50/60 Hz, 1,4 A

Internal protection of the secondary circuit: 1 x T4AH; 1 x T2AH

Weight: control unit: 2,4 kg

STS complete: 4,4 kg

disposable blade carrier TE: 2,8 kg

Dimensions of the STS: Ø 300 mm x height: 130 mm

Dimensions of the control unit: 265 x 160 x 80 mm (long x wide x high)

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