Thermo Scientific SimpliAmp Guía de inicio rápido

SimpliAmp™Thermal Cycler PCR System Installation and

Operation

Catalog Number A24811

Pub. No. A24827 Rev. D

Note: For safety and biohazard guidelines, see the “Safety”

appendix in the SimpliAmp™ Thermal Cycler User Guide (Pub. No.

MAN0009889). Read the Safety Data Sheets (SDSs) and follow the

handling instructions. Wear appropriate protective eyewear,

clothing, and gloves.

Product description

This document summarizes procedures for installing and using

the SimpliAmp™ Thermal Cycler, with "beer than gradient™"

VeriFlex™ technology. For detailed instructions, see the SimpliAmp™

Thermal Cycler User Guide (Pub. No. MAN0009889).

Figure 1 SimpliAmp™Thermal Cycler

Required materials not provided

• Can of compressed air (For use in cleaning wells of sample

block).

•(Optional) Electrical protective devices.

Note: The use of one or more of the following electrical

protective devices is recommended.

·Power line regulator (100–240 V)

·Surge protector/line conditioner (10-kVA)

·Uninterruptible power supply (1.5-kVA)

Set up the SimpliAmp™Thermal Cycler

IMPORTANT! Save the packing materials and box in case you

need to ship the instrument in for service.

1. Open the shipping crate to unpack the instrument. You

should receive one box containing the thermal cycler and the

accessories.

2. Remove the packing material, then inspect the instrument for

shipping damage.

3. Use compressed air to clear out each well of the sample block

to remove particles that may have collected inside during

shipping.

4. Place the instrument at an installation site that meets the

spatial and weight requirements for the thermal cycler (see

“Technical specications“ on page 6).

5. Connect the instrument to a power outlet.

a. Connect the power cord to the instrument.

1Power cord port

b. Install any desired electrical protective devices.

c. Connect the power cord to a wall plug.

6. Press the power buon, then wait for the instrument to start

up. Proceed with the installation after the touchscreen

displays the Home screen, indicating that the instrument is

active.

When you power on the instrument, the instrument will take

under a minute to start up.

QUICK REFERENCE

For Research Use Only. Not for use in diagnostic procedures.

1Power switch

The Home screen is displayed upon successful installation.

Figure 2 SimpliAmp™Home screen

Connect the PCR instrument to the Internet

Connect your thermal cycler to the Internet. See the instrument

user guide for detailed instructions.

• Connect through the instrument Ethernet port using a cable.

• Connect via wireless connection with the High-Power USB Wi-

Fi Module (Cat. No. A26774).

Set up mobile and cloud connectivity to the

SimpliAmp™Thermal Cycler and your mobile

device

Create a user proﬁle on the PCR instrument

1. Press (Sign In) > Get started > Create prole.

2. Fill in the required text elds and follow the prompts to

create your user prole.

Create a Thermo Fisher Connect account

1. Go to thermosher.com/connect from your web browser.

2. Click Sign up now and follow the prompts to create an

account.

Link the PCR instrument to Thermo Fisher Connect

(administrator only)

The procedure to link the Administrator prole to Thermo Fisher

Connect only needs to be performed the rst time the PCR

instrument is added to Thermo Fisher Connect.

1. Press (Sign In) > Sign In, then select the Administrator

prole.

2. Log in. (The default password for the Administrator prole is

"password").

You will then be redirected back to the home screen.

3. Press (Seings) > Instrument Seings > Remote Service.

4. Set Remote Service to "On", then press OK.

5. Press (My Prole) on the home screen.

6. Select Cloud, then enter the Thermo Fisher Connect login

and password associated with the Administrator prole.

7. Press Link Account.

Upon successful authentication, the Administrator prole is

linked to Thermo Fisher Connect.

Note: If an instrument is already linked to Thermo Fisher

Connect, you will have the option to unlink the instrument if

desired.

SimpliAmp

™

Thermal Cycler Installation and Operation Quick Reference

Add a PCR instrument to your Thermo Fisher Connect

account

Note: Multiple instruments can be added to your Thermo Fisher

Connect account, but only one user prole from each instrument

can be linked to your account.

1. Press (Sign In) > Sign In, then select your user prole.

2. Log in to your user prole.

You will then be redirected back to the home screen.

3. Press (Seings) > Instrument Seings > Remote Service.

4. Set Remote Service to "On", then press OK.

5. Press (My Prole) on the home screen.

6. Select Cloud, then enter your Thermo Fisher Connect

account login and password.

7. Press Link Account.

Upon successful authentication, the the user prole is linked

to Thermo Fisher Connect.

Set up mobile connectivity on a mobile device

Download the "Instrument Connect Mobile Application" on your

mobile device.

1. For iPad™ or iPhone™ devices, download the application from

the iTunes™ music store by searching for Instrument Connect

by Thermo Fisher Scientic.

2. For Android devices, download the application from

Google™ Play by searching for Instrument Connect by

Thermo Fisher Scientic.

3. Log in to the instrument connect mobile application using

your Thermo Fisher Connect login and password .

How to use the SimpliAmp™Thermal Cycler

Operate the instrument using the touchscreen. For detailed

instructions on using the thermal cycler, see the SimpliAmp™

Thermal Cycler User Guide (Pub. No. MAN0009889).

In the Home screen for the touchscreen, you can set up a run by

creating a new method for a run. To set up a run using a new

method:

1. Press New Method or Set Up Run.

2. Select a template.

3. In the edit mode, edit the parameters of the method template

such as temperature, time, number of steps/stages.

4. To access advanced editing options, press Manage Steps >

Advanced Options. Advanced editing options include

VeriFlex™ blocks for optimization, simulation modes, ramp

rates, and AutoDelta.

5. Save the new method in a folder.

6. (Optional) Press Start Run to start the Method.

SimpliAmp

™

Thermal Cycler Installation and Operation Quick Reference

Maintenance guidelines

CAUTION! During instrument operation, the temperature

of the heated cover can be as high as 110°C, and the

temperature of the sample block(s) can be as high as 100°C.

Before performing the procedure, keep hands away until

the heated cover and sample block(s) reach room

temperature.

To ensure proper operation:

• Regularly:

– Wipe the instrument surfaces with a lint-free cloth.

– Clean the vents, touchscreen, and sample block of the

instrument.

– Clean the sample wells with 100% isopropanol.

• Use only consumables recommended by Thermo Fisher

Scientic for the instrument.

Use of consumables that are larger or smaller than the

specied volume can damage the instrument, contaminate

the sample block, and/or decrease the PCR yield (due to

inecient thermal transfer).

•Do not use sharp objects on the touchscreen.

Use only your ngers or blunt objects to enter commands on

the instrument touchscreen. Sharp and/or pointed objects

such as writing utensils can damage the surface of the

touchscreen.

•Back up frequently.

Routinely back up the congurations and les on your

instrument to a USB drive. Regular backups protect against

data loss caused by user error, power failure, or instrument

error. For more information, see the SimpliAmp™ Thermal

Cycler User Guide (Pub. No. MAN0009889).

How to use the MicroAmp™96-well Tray and

Retainer

IMPORTANT! Use the MicroAmp™ 96-well Tray and Retainer to

prevent crushing of tubes in the thermal cycler.

When small number of tubes (1–2 tube strips or ≤8–16 tubes are

placed in the sample block without support, they can be crushed

or deformed when the lid of the thermal cycler is closed over the

block. Using the tray and retainer set allows the pressure to be

distributed evenly over the tubes and prevents uneven pressure

across the block.

Using the tray and retainer set is optional when using ≥3 tube

strips or ≥16 tubes that are distributed evenly across the block.

• The blue tray and retainer set is compatible for use with

MicroAmp™ single tubes or tube strips with separate cap

strips.

• The blue tray by itself is compatible for use with MicroAmp™

tube strips with aached caps.

• The black retainer is compatible for use with single

MicroAmp™ reaction tubes with aached caps.

Prepare samples using MicroAmp™tubes/tube strips

with separate cap strips

1. Separate the blue tray and retainer by squeezing the release

catch as indicated in the graphic.

1Release catch

2MicroAmp™96-Well Retainer

3MicroAmp™96-Well Tray

2. Place the blue tray on the 96-well base.

3. Load the tube strips on the tray.

4. Pipee the reaction mixture into the tubes.

5. Place the blue retainer over the tubes and snap the retainer

into the tray.

6. Seal the tube strip using a MicroAmp™ cap strip. See “Seal

tubes strips with cap strips“ on page 5 for instructions.

7. Remove the blue tray/retainer assembly containing the

sealed tube strips from the 96-well base and place the

assembly into the instrument.

1MicroAmp™8-Cap strip

2MicroAmp™96-Well Retainer

3MicroAmp™8-Tube Strip (0.2-mL) or MicroAmp™Reaction

Tube without Cap (0.2-mL)

4MicroAmp™96-Well Tray

5MicroAmp™Splash Free 96-Well Base

SimpliAmp

™

Thermal Cycler Installation and Operation Quick Reference

Prepare samples using MicroAmp™tube strips with

attached caps

1. Separate the blue tray and retainer by squeezing the release

catch as indicated in the graphic.

1Release catch

2MicroAmp™96-Well Retainer

3MicroAmp™96-Well Tray

2. Place the blue tray on the 96-well base.

3. Load the tube strips on the tray.

4. Pipee the reaction mixture into the tubes.

5. Seal the tube strip using a MicroAmp™ cap strip. See “Seal

tubes strips with cap strips“ on page 5 for instructions.

6. Remove the blue tray containing the sealed tube strips from

the 96-well base and place the tray and sealed tube strips into

the instrument.

1MicroAmp™8-Tube Strip with Attached Caps (0.2-mL)

2MicroAmp™96-Well Tray

3MicroAmp™Splash Free 96-Well Base

Seal tubes strips with cap strips

IMPORTANT! Apply signicant downward pressure on the

sealing tool in all steps to form a complete seal on top of the tubes.

1. Align and place the cap strips on the tubes.

2. Seal the cap strips using the rocking capping tool:

a. Slip your ngers through the handle with the holes in

the tool facing down.

b. Place the holes in the tool over the rst eight caps in a

row.

c. Rock the tool back and forth a few times to seal the

caps.

d. Repeat for remaining caps in the row, then for all

remaining rows.

Prepare samples using MicroAmp™Reaction Tubes

1. Set the black tray on a 96-well base.

2. Place the reaction tubes in the black tray.

1MicroAmp™Reaction Tube with Cap (0.2-mL)

2MicroAmp™96-Well Tray for VeriFlex™Blocks

3MicroAmp™Splash Free 96-Well Base

3. Pipee the reaction mixture into the reaction tubes.

4. Cap the tubes.

5. Remove the black tray with sealed reaction tubes from the

96-well base and place the tray and sealed tubes into the

instrument.

SimpliAmp

™

Thermal Cycler Installation and Operation Quick Reference

Technical speciﬁcations

The following table displays the system specications for the SimpliAmp™ Thermal Cycler.

Table 1 SimpliAmp™Thermal Cycler technical speciﬁcations:

Feature Speciﬁcation

Maximum block ramp rate[1] 4°C/sec

Maximum sample ramp rate[1] 3°C/sec

Temperature accuracy ±0.25°C (35 to 99.9°C)

Temperature range for

protocol run

0°C to 100.0°C

Temperature non-uniformity[2] <0.5°C

Dimensions • Height: 21 cm (8.27 in)

• Width: 24.0 cm (9.45 in)

• Depth: 46 cm (18.11 in)

Weight 8.5 kg (18.3 lb)

PCR volume range • Supported: 10 - 100 µL

• Settable: 1 - 100 µL

Instrument memory USB, on-board

Display interface 8" color TFT LCD

Power 100-240 V, 50-60 Hz Max: 600 W

VeriFlex™blocks • 3 VeriFlex™zones

• Supported 10°C (5°C zone-to-zone)

• Settable[3] 20°C (10°C zone-to-zone)

Ambient humidity 15% - 80% Relative Humidity, non-condensing

(acceptable range)

Ambient operating

temperature

15°C to 30°C (acceptable range)

[1] Reaction volume @ 1 µL

[2] 30 seconds after clock start

[3] Temperature accuracy ±0.5°C and Temperature non-uniformity < 0.75°C

Table 2 Power and communication ports

Port Description

AC power cable port

10/100 Fast Ethernet port for connecting to a network

USB v2.0 port for connecting to an external network drive, jump drive, or other USB storage device

RS232 serial communication port for connecting the probe for the Temperature Veriﬁcation Kit

USB v2.0 port for connecting to USB-enabled Wi-Fi Card

SimpliAmp

™

Thermal Cycler Installation and Operation Quick Reference

Accessory products

Product Cat. No.

96-well 0.2-mL reaction plates

MicroAmp™Optical 96-Well Reaction Plate N8010560, 4316813

MicroAmp™Optical 96-Well Reaction Plate with Barcode 4306737, 4326659

MicroAmp™Optical 96-Well Reaction Plate with Barcode & Optical Caps 403012

MicroAmp™Optical 96-Well Reaction Plate with Barcode & Optical Adhesive Films 4314320

MicroAmp™EnduraPlate™Optical 96-Well Clear Reaction Plates with Barcode 4483354, 4483352

MicroAmp™TriFlex 3 x 32-Well PCR Reaction Plate A32810, A32811

96-well 0.1-mL reaction plates

MicroAmp™Fast Optical 96-Well Reaction Plate, 0.1 mL 4346907

MicroAmp™Fast Optical 96-Well Reaction Plate with Barcode, 0.1 mL 4346906, 4366932

MicroAmp™EnduraPlate™Optical 96-Well Fast Clear Reaction Plates with Barcode 4483485, 4483494

384-well reaction plates

MicroAmp™Optical 384-Well Reaction Plate 4343370

MicroAmp™Optical 384-Well Reaction Plate with Barcode 4309849, 4326270, 4343814

MicroAmp™EnduraPlate™Optical 384-Well Clear Reaction Plates with Barcode 4483285, 4483273

Strip tubes and caps

MicroAmp™Fast 8-Tube Strip, 0.1 mL 4358293

MicroAmp™Optical 8-Tube Strip with Attached Optical Caps, 0.2 mL A30588

MicroAmp™Optical 8-Tube Strip with Attached Domed Caps, 0.2 mL A30589

MicroAmp™8-Tube Strip, 0.2 mL N8010580

MicroAmp™Optical 8-Tube Strip, 0.2 mL 4316567

MicroAmp™8-Cap Strip, clear N8010535, N8011535

MicroAmp™Optical 8-Cap Strips 4323032

MicroAmp™12-Cap Strip N8010534, N8011534

Single Tubes

MicroAmp™Fast Reaction Tube with Cap, 0.1 mL 4358297, 4358293

MicroAmp™Reaction Tube with Cap, 0.2 mL N8010540, N8011540, N8010612

MicroAmp™Reaction Tube without Cap, 0.2 mL N8010533, N8011533

MicroAmp™Optical Tube without Cap, 0.2 mL N8010933

Seals and covers

MicroAmp™Clear Adhesive Film 4306311

MicroAmp™Optical Adhesive Film 4360954, 4311971

MicroAmp™32-Well Clear Adhesive Film A32812

Accessories

MicroAmp™Splash-Free 96-Well Base 4312063

MicroAmp™96-Well Support Base 4379590

MicroAmp™96-Well Base N8010531

MicroAmp™96-Well Reaction Tube/Tray/Retainer Set, 0.2 mL 403083, 403086

SimpliAmp

™

Thermal Cycler Installation and Operation Quick Reference

Limited product warranty

Life Technologies Corporation and/or its aliate(s) warrant their products as set forth in the Life Technologies' General Terms and

Conditions of Sale found on Life Technologies' website at www.thermosher.com/us/en/home/global/terms-and-conditions.html. If you

have any questions, please contact Life Technologies at www.thermosher.com/support.

Manufacturer: Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256

The information in this guide is subject to change without notice.

DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,

PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these products, you accept the terms and conditions of all

applicable Limited Use Label Licenses.

thermoﬁsher.com/support | thermoﬁsher.com/askaquestion

thermoﬁsher.com

25 September 2018

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