Tecella Triton Plus Manual de usuario

3001 Red Hill Ave, Suite 1-204

Costa Mesa, CA 92626

Phone +1-714-641-1709

Fax +1-714-641-1569

Triton+ User’s Manual

Table of Contents

Before You Begin.....................................................................................................3

1.1 Introduction................................................................................................3

1.2 Package Contents.....................................................................................3

1.4 Computer Requirements..........................................................................3

1.5 Installation Overview.................................................................................3

Installation................................................................................................................4

2.1 Select the Driver Setup File for Your OS.................................................4

2.2 Install Driver File Onto Your Computer....................................................5

2.3 Connect the Power Supply To the Amplifier............................................7

2.4 Plug In the Power Supply Into an AC Outlet ...........................................8

2.5 Connect the USB Cable To the Amplifier and the Computer.................9

2.6 Turn On the Amplifier................................................................................9

2.7 Associating the Driver and the Amplifier (Windows XP).......................10

Getting Started With TecellaLab...........................................................................13

3.1 Install TecellaLab....................................................................................13

3.2 Launch TecellaLab..................................................................................15

3.3 Start Acquisition ......................................................................................16

3.4 Changing Gain (Rf setting).....................................................................17

3.5 Changing Source....................................................................................18

3.8 Save Data To a File................................................................................19

3.9 Convert Data to Tab Delimited or ATF Format.....................................22

3.10 Using TecellaLab..................................................................................22

Getting Started With WinWCP..............................................................................24

4.1 Download WinWCP Setup File..............................................................24

4.2 Download WinWCP User Guide............................................................24

4.3 Install WinWCP.......................................................................................24

4.4 Start WinWCP.........................................................................................24

4.5 Set Laboratory Interface Card to “Tecella Triton”..................................25

4.3 Using WinWCP.......................................................................................26

Connecting To Your Setup....................................................................................27

Using TecellaLab...................................................................................................28

Hardware Calibration.............................................................................................29

6.1 Check For Hardware Drift.......................................................................29

6.2 Option 1: Re-start the Application.........................................................29

6.3 Option 2: Re-run Hardware Calibration................................................30

Specifications.........................................................................................................32

7.1 Source Selector.......................................................................................32

7.2 Headstage and Feedback Gain Resistor (Rf).......................................32

7.3 Current Range & Current Resolution.....................................................33

7.4 A/D Resolution & Sampling Rate...........................................................33

7.5 Analog Filter ............................................................................................33

7.6 Noise........................................................................................................34

7.7 Capacitance Compensation...................................................................35

7.8 Series Resistance Compensation..........................................................36

7.9 Analog Leak Compensation (optional) ..................................................36

7.10 Command Voltage................................................................................36

7.11 Holding Voltage.....................................................................................36

7.12 Junction Offset Compensation.............................................................37

7.13 Zap.........................................................................................................37

7.14 Sync Out................................................................................................37

7.15 Trigger In...............................................................................................37

7.16 Acquisition Modes.................................................................................37

7.17 Acquisition Data Format.......................................................................37

7.18 Power Supply........................................................................................37

7.19 Power Requirements............................................................................37

7.20 Mechanical Dimensions .......................................................................37

Troubleshooting & Support....................................................................................38

Technical Support.............................................................................................38

Chapter

1 Before You Begin

1.1 Introduction

The Triton+ is a full-featured whole cell patch clamp amplifier, available in 1, 2, 4, or 8 channel

configurations. The digitizer, the head stages, and the model cells are all integrated inside the

Triton+ amplifier simplifying setup of the work environment. Triton+ communicates to the

computer via a standard USB 2.0 cable, so no special hardware is required on the computer.

1.2 Package Contents

Please confirm that the following items were included in the Triton+ package.

 Triton+ amplifier

 Power supply

 USB cable (Mini-USB)

What You May Need To Provide

Since AC outlets vary depending on your country, you may need to provide your own

AC chord to connect between the power supply and the AC outlet.

1.4 Computer Requirements

The following is the minimum computer requirements for using the Triton+.

 2GHz single core processor or 1.4GHz dual core processor

 1GB RAM

 10GB free space on your Hard Drive

 One available USB 2.0 port

 Windows XP, Vista, or 7

1.5 Installation Overview

The Triton+ setup can be summarized in the following 5 steps.

1. Install Driver file

2. Install Software

3. Plug in power supply

4. Plug in USB cable

5. Turn on the amplifier to associate Driver with the amplifier

These steps will be explained in greater detail in the following two chapters.

Chapter

2 Installation

2.1 Select the Driver Setup File for Your OS

Before plugging in the amplifier to the computer for the first time, please follow the following

steps to install the FrontPanel driver file onto your computer. The FrontPanel driver file comes

in 32-bit version and 64-bit version. Please install the correct version that matches your

operating system (OS). You can check which version OS you are running by opening Control

Panels and selecting System. If you do not see any mention of 64-bit Operating System, then

please choose the 32-bit driver file.

Previous Driver Installations

If you have previously installed the FrontPanel driver onto your computer, there is no

need to reinstall the driver file, and you can skip this section and the next section on

FrontPanel driver file installation.

Driver Setup File for 32-bit Windows XP, Vista, 7

For 32-bit Windows XP, Vista, or 7 OS, please use the file called

“FrontPanel-DriverOnly-Win-Win32-3.1.0.exe”. Double click on the

icon to begin installing the driver files.

Driver Setup File for 64-bit Windows XP, Vista, 7

For 64-bit Windows XP, Vista, or 7 OS, please use the file called

“FrontPanel-DriverOnly-Win-x64-3.1.0.exe”. Double click on the icon

to begin installing the driver files.

2.2 Install Driver File Onto Your Computer

Follow the steps below to install the FrontPanel driver file onto your computer.

First, you will see the Setup Wizard

welcome screen. Click on “Next” to

proceed.

Next, you will see Opal Kelly’s

License Agreement. Click on “I

Agree” to install the driver.

Depending on the security setting of

your computer, you may see this

warning screen. Click on “Install this

driver software anyway” to continue

with installation.

This screen may momentarily

appear during driver installation.

After the FrontPanel driver files has

been successfully installed on your

computer, you will see this

confirmation screen. Click on

“Finish” to exit the installation.

Although the driver files have been

installed on your computer, they

have not been associated with the

amplifier yet. The driver file and the

amplifier will be associated the first

time the amplifier is connected to the

computer and turned on.

This warning screen may appear on

computers running Windows Vista

and Windows 7, even if the

installation was successful. Click on

“Cancel” to exit the warning screen.

2.3 Connect the Power Supply To the Amplifier

Locate the connector of the power supply.

Locate the power jack on the back of the amplifier as indicated by the arrow below.

Insert the power connector firmly into the power

jack. The power connector has a locking

mechanism, so it is important that the power

connector is fully inserted into the power jack.

Test for Proper Insertion

Considerable force is required to properly insert the power connector and engage the

locking mechanism. To test for proper insertion, pull snugly on the cable. If the

connector gets loose, the insertion force was insufficient to lock the connector. Please

try again by applying more insertion force.

Power

Connector

Power

Jack

Caution! Extremely Important When Detaching Power Cable

When detaching the power cable from the

amplifier, in order to disengage the locking

mechanism, you must first pull back on the

connector cover as shown.

As you pull back on the connector cover, the silver

colored shield should become visible. Continue

pulling on the connector to detach the power cable.

NEVER attempt to detach the power connector by

pulling on the cable or on the stress relief of the

cable.

2.4 Plug In the Power Supply Into an AC Outlet

Plug in the power supply into an AC outlet using the appropriate AC chord for your country’s

outlet.

NO !

YES

2.5 Connect the USB Cable To the Amplifier and the Computer

A standard Mini-USB cable (as shown) is included

with the amplifier.

Plug the small end of the USB cable into the USB port of the amplifier indicated below.

Plug the large end of the USB cable into the USB port of your computer. The USB port must

be USB 2.0.

2.6 Turn On the Amplifier

Turn on the amplifier by toggling the power switch indicated below.

USB

Power

Switch

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