Qsonica SONICATOR S-4000 Manual de usuario
SONICATOR
ULTRASONIC PROCESSOR
Part No. S-4000
OPERATION MANUAL
53 Church Hill Road
Newtown, CT 647
2 3.426. 1 1 tel
2 3.426.7 26 fax
www.sonicator.com
2
Table of Contents
Section Page No.
1. Warnings 4
2. Specifications 5-6
3. Principles of Operation 7
4. escription of Components 8-10
5. Preparation for Use (Getting Started) 11-14
6. Operating Instructions 15-24
7. Accessories 25-26
8. Sound Reducing Enclosure 27-29
9. Techniques for Optimizing Results 30
10. Maintenance 31-36
11. Troubleshooting 37-38
12. Warranty 39
13. Return of Equipment 40
14. Instructions for Specialty Horns 41-55
a. Cup Horn 41-43
b. Microplate Horn 44-49
c. Flocells™ 50-53
d. ual Horn
54-55
3
Please read the manual in its entirety. The new digital Sonicator offers
several new and innovative features. This manual provides the
necessary instruction and guidance to help ensure the successful
operation of this device.
4
1. Warnings
Your new Ultrasonic iquid Processor has been designed, built and tested to assure
maximum operator safety. However, no design can completely protect against improper use
that may lead to bodily injury and/or property damage. For total safety and equipment
protection, read the instruction manual carefully before attempting to operate this equipment.
Observe the following WARNINGS:
High voltage is present in the generator (power supply), converter and high frequency cable. There are no
user-serviceable parts inside any of these devices. Do NOT attempt to remove the generator cover or
converter case.
Do NOT touch any open cable connections on the unit while the power is turned ON.
Do NOT operate generator with converter disconnected from high voltage cable. High voltage is present in
the cable and may pose a shock hazard.
Do NOT attempt to disconnect the converter high voltage cable while the unit is running.
The generator must be properly grounded with a 3-prong plug. Test electrical outlet for proper grounding
before plugging in unit.
Install the SONICATOR
®
in an area free from excessive dust, dirt, explosive or corrosive fumes and
protected from extremes in temperature and humidity. Do not place the Generator within a Fume Hood.
NEVER immerse the converter in liquids of any kind, or let condensed moisture or liquid drip into the
converter.
NEVER grasp an activated horn or touch the tip of a vibrating probe. It can cause severe burns and tissue
damage.
NEVER hold or clamp the converter by the front driver (black portion) or by the horn itself. This can cause
permanent damage to the system. Support the converter by only clamping around the converter case.
Do NOT allow the tip of a vibrating horn or probe to touch the counter top or any other hard surface. It could
damage the probe, overload the generator, or damage the surface.
Avoid touching the bottom or sides of a glass or plastic container with an activated probe. It could crack or
shatter the glass or melt the plastic.
Turn OFF the power switch, unplug the generator and disconnect the power cord from the back of the
generator before attempting to replace the fuses.
Inspect high frequency cable for cracks in the protective outer jacket.
Do not operate unit with a damaged cable. Doing so may cause serious injury.
In case of AC power loss, wait 3 minutes minimum before reapplying power.
Do not turn off AC mains power while running a horn. Stop sonication through the touch screen prior to
removing AC power.
Symbols
Caution, Risk of electric shock, Hazardous voltage.
5
Caution, Risk of danger. Refer to User Manual.
2. Specifications
Input Voltage
100 VAC -240 VAC @ 50/60 Hz
Rated Current
100 VAC – 120 VAC, 7 Amps max.
220 VAC – 240 VAC, 3.5 Amps max.
Fuse Rating
For 100 VAC – 120 VAC, 8 Amps (Fast Acting)*
For 220 VAC – 240 VAC, 4 Amps (Fast Acting)*
Weight
13.5 lbs. (6.1Kg)
Dimensions
11"W x19.5" x 5.25"H
27.9cm x 49.5cm x 13.3cm
Output Voltage
1000 V rms (max.)
Output Frequency
20 KHz (nom.)
Converter
Weight
2 lbs. (0.9 Kg)
Dimensions
8" x 2.5" Dia.
(20.3cm x 6.4cm)
Materials
Aluminum
Standard ½” Horn
Weight
0.5 lbs. (0.45Kg)
Dimensions
5" x1.5" Dia.
(12.7cm x 3.8cm)
Materials
Titanium Alloy
6
Environmental
Pollution Degree
2
Installation Category
II
Operating imits
Shipping/Storage
Temperature: 39 - 95
o
F (4 - 35
o
C)**
Relative Humidity 20 - 90% (Non Condensing)
Temperature: 35 -120
o
F (2 - 49
o
C)
Relative Humidity 10 - 95% (Non Condensing)
Ambient Pressure Extremes: 40,000 ft.
Restriction of
Hazardous
Substances (ROHS)
Relative humidity Maximum relative humidity 80% for temperatures up to 31ºC decreasing
linearly to 50% relative humidity to 40ºC
Other For indoor use only
Regulatory
U , CU , CE
Evaluated to U 61010-1: 2
nd
edition, IEC 61010-1, CAN/CSA – C22.2 No.
61010-1 second edition (IEC 61010-1, Mod)
*Only use IEC approved Fast acting fuses, Cooper Bussman series S500.
**At low temperatures the screen contrast may require adjustment. See Option menu.
The Power Cord supplied with the Sonicator must be used. If the 220V plug is not configured to
match the wall receptacle, a properly grounded universal AC socket adapter must be added.
Important: Universal adapters do not convert voltage or frequency. QSonica is not responsible for
damage caused by the use of an improper power cord or adapter. Transformers are not
recommended.
WEEE Statement
This product contains electrical or electronic materials. The presence of these
materials may, if not disposed of properly, have potential adverse effects on the
environment and human health. Presence of this label on the product means it should
not be disposed of as unsorted waste and must be collected separately. As a
consumer, you are responsible for ensuring that this product is disposed of properly.
To find out how to properly dispose of this product contact Customer Service at
info@misonix.com.
7
3. Principles of Operation
The ultrasonic electronic generator transforms AC line power to a 20 KHz signal that drives a
piezoelectric converter/transducer. This electrical signal is converted by the transducer to a
mechanical vibration due to the characteristics of the internal piezoelectric crystals.
The vibration is amplified and transmitted down the length of the horn/probe where the tip
longitudinally expands and contracts. The distance the tip travels is dependent on the
amplitude selected by the user through the touch screen pad. As you increase the amplitude
setting the sonication intensity will increase within your sample.
In liquid, the rapid vibration of the tip causes cavitation, the formation and violent collapse of
microscopic bubbles. The collapse of thousands of cavitation bubbles releases tremendous
energy in the cavitation field. The erosion and shock effect of the collapse of the cavitation
bubble is the primary mechanism of fluid processing.
The probe tip diameter dictates the amount of sample that can be effectively processed.
Smaller tip diameters (Microtip
™
probes) deliver high intensity sonication but the energy is
focused within a small, concentrated area. arger tip diameters can process larger volumes,
but offer lower intensity.
The choices of a generator and horns/probes are matched to the volume, viscosity and other
parameters of the particular application. Horns are available for both direct and indirect
sonication. The Accessories section has more information on this subject.
Ultrasonic processors have proven to be highly effective for many applications including:
• Cell disruption
• Nanoparticle dispersion
• Homogenization
• Shearing DNA
• Disaggregation
• Focused cleaning
• Mixing
• Degassing
• Catalyzing reactions
Please consult with a QSonica product specialist for help with selecting the proper generator
and horn for your application.
8
4. escription of Major Components
Generator
The Generator supplies a 20 KHz electrical signal to the converter. The S-4000’s advanced
digital signal processing tracks frequency changes in the Probe-Converter Assembly caused
by load and temperature changes, and maintains maximum electrical efficiency at all times.
The Generator also incorporates an enhanced digitally controlled automatic gain feature that
maintains the amplitude selected by the user. As the Probe is loaded, the S-4000 will
automatically supply greater power (wattage) to the Converter, thereby keeping the
amplitude constant. Amplitude, which is the distance the Probe’s tip moves out from rest and
back in each cycle, determines the level of sonication.
The S-4000 also contains a self test and diagnostic feature that acts as a signal analyzer in
determining the integrity of the system. The S-4000 is equipped with fault detection circuits
that protect the generator from overloads due to defective converter / probe assemblies. The
self test and diagnostic feature can be activated following an error condition to assist the user
in remedying the fault or from one of the menu screens.
Also employed in this design is a CD touchscreen user interface that contains easy to use
menus for the control and operation the system. The S-4000 can either be controlled
manually or automatically through user defined programs. Output amplitude can be set from
1% -100% giving a greater degree of resolution for setting the amplitude needed to
effectively process samples. Reference information such as converter power output, joules,
and process time is also displayed.
Incorporated into the S-4000 is a universal power supply that recognizes any AC mains
voltage throughout the world. The back panel of the S-4000 contains Mains power inlet and
fuse drawer, high voltage BNC connector for the converter cable, footswitch and K-type
temperature probe input connectors. In addition, a USB input connector is available for easy
field software upgrades.
Converter (also known as the Transducer)
The converter consists of a cylindrical unit (mounting area), and black colored front driver
(the first stage of acoustic amplification) with a threaded hole for horn attachment. The
generator supplies a 20 KHz electrical signal to the converter. The Converter changes the
signal to mechanical vibration.
Horn (also known as the Probe)
A horn is an approximately 5” long resonant body that steps down from 1.5” diameter to a
specific tip diameter. The horn serves as the second stage of acoustic amplification. The
horn may be solid or have a removable tip and is supplied with a threaded stud for
attachment to the converter and external threads around the body for attachment to other
accessories. Horns are available in three standard tip diameters of ½”, ¾” or 1" and come in
three different tip configurations. Tapped horns have a replaceable Flat Tip; Solid horns are
one solid piece and do not have a replaceable tip; and Sapphire tipped horns have a 1/16"
thick sapphire disk bonded to the tip of a solid horn.
9
The # S-4000 includes a standard ½” diameter tapped horn (#200).
Generator
Converter
½” Horn
with
replaceable tip
Converter
Cable
10
S-4000 Rear Panel
On/Off
Switch
Power Cord
Connector
Converter
Cable
Connector
USB
Connector
Temperature
Probe
Connecto
r
Footswitch
Jack
Cooling Fan
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