PSI FMT150 Guía

Photobioreactor FMT150

Manual and User Guide

Please read this manual before operating the product.

TABLE OF CONTENTS
Warnings and Safety Precautions.....................................................................................................................................4
 . General Description..........................................................................................................................................................5
Device Description............................................................................................................................................................6
1 Configurations and Versions.....................................................................................................................................8
 Standard Components............................................................................................................................................11
3 Control Unit Front Panel.........................................................................................................................................14
4 Rear Panel for version FMT150.1............................................................................................................................16
5 Rear Panel for version FMT150. ............................................................................................................................18
6 Connection Schema................................................................................................................................................19
Device Installation........................................................................................................................................................... 1
1 Power Supply........................................................................................................................................................... 1
 Cultivation Vessel.................................................................................................................................................... 
3 pH/Temperature Sensor.......................................................................................................................................... 6
4 Gas Supply............................................................................................................................................................... 8
Optional Accessories/Components................................................................................................................................30
1 PBR Software...........................................................................................................................................................30
 Air Pump with Bubble Interruption Valve...............................................................................................................30
3 pH/Temperature Module........................................................................................................................................31
4 O  Module...............................................................................................................................................................31
5 CO  Module.............................................................................................................................................................3 
6 Magnetic Stirrer......................................................................................................................................................40
7 Gas Mixing System GMS 150...................................................................................................................................45
8 Gas Analysis System GAS 150..................................................................................................................................48
9 Peristaltic Pump PP500...........................................................................................................................................5 
10 External Light Panel...............................................................................................................................................56
11 Additional Software License.................................................................................................................................57
1  Light Upgrade........................................................................................................................................................57
13 Supplementary/Replacement Components.........................................................................................................57
Cultivation Guide............................................................................................................................................................59
1 Set Up the Components..........................................................................................................................................59
 Set Up the Experiment............................................................................................................................................59
Troubleshooting and Customer Support........................................................................................................................61
Appendix.........................................................................................................................................................................6 
1 FMT150 Control Menu Tree....................................................................................................................................6 
Page  

8. QY measurement....................................................................................................................................................70

8.3 Technical Specification Standards...........................................................................................................................71

9. Warranty Terms and Conditions.....................................................................................................................................75

10. List of Abbreviations.....................................................................................................................................................78

Page 3

1.Warnings and Safety Precautions

1. WARNINGS AND SAFETY PRECAUTIONS

PLEASE READ THE FOLLOWING INSTRUCTIONS CAREFULLY BEFORE TURNING THE PHOTOBIOREACTOR ON:

•Remove all packaging and transport protectors before connecting the photobioreactor to the power supply.

•Use only cables supplied by the manufacturer.

•Keep the device dry outside and avoid working in high humidity environment!

•The manufacturer is not responsible for any damage due to improper operation!

•Water and other liquids should only be placed in vessels designed for the purpose and according to

instructions included in this manual.

GENERAL ELECTRICAL SAFETY GUIDELINES:

•Perform a routine check of the devices and their wiring.

•Replace worn or damaged cords immediately.

•Use appropriate electrical extension cords/power bars and do not overload them.

•Place the device on a flat and firm surface. Keep away from wet floors and counters.

•Avoid touching the device, socket outlets or switches if your hands are wet.

•Do not perform any alterations to the electrical parts of the device or its components.

Warning:

The Photobioreactor is considered Class 1M* LED Product. LED radiation may be harmful to eye. Avoid direct

and strongly reflected exposure. Use protective glasses.

*Class 1M: Laser and LED equipment that is safe for the naked eye under foreseeable conditions of operation.

Looking directly into the source of radiation by employing optics ithin the beam, such as magnifying glass,

telescope or microscope, can be potentially hazardous.

Page 4

.General Description

2. GENERAL DESCRIPTION

Photobioreactor FMT150 feature a unique combination of the cultivator and computer controlled monitoring device.

The Photobioreactor FMT150 combines a cultivation vessel with the built-in fluorometer and densitometer. It is

primarily designed for modular and precise phototrophic cultivation of algae, cyanobacteria and bacteria. Light power

and spectral composition as well as temperature and aeration gas composition can be set with high accuracy.

User defined cultivation conditions can be dynamically varied according to user defined protocol. Continuous online

monitoring of cultivation conditions and measured parameters is possible via the optional PBR software. Light,

temperature, gas composition, and culture medium regime can be programmed to oscillate with various amplitudes

and frequencies with user-defined time steps from milliseconds to hours via the software or via the control unit.

Turbidostatic cultivation can be used for the stabilization of the suspensions via optical density control. In addition, up

to 8 peristaltic pumps for different chemostat cultivation control can be linked to the Photobioreactor FMT150 for the

highly precise control of cultivation conditions.

The growth of the cultures is monitored by the integrated densitometer (OD 7 0, 680 nm). Relative chlorophyll

content of the culture can also be monitored continuously by the difference of optical densities at 680 and 7 0 nm.

The instantaneous physiological state of the culture is measured by the Photosystem II quantum yield Fv/Fm.

The Photobioreactor FMT150 has a flat-vessel design that enables uniform illumination over the whole culture.

Photobioreactors are currently manufactured in three cultivation vessel capacities: 400 ml, 1000 ml and 3000 ml.

All Photobioreactor models may be supplied with a number of optional accessories – sensors, stirrers, pumps, etc.

Control of the Photobioreactor is possible with either the control unit of the photobioreactor itself or the PBR

software. However, the PBR software provides much wider functionality to define, control, display and analyze the

experimental conditions and data.

Page 5

3.Device Description

3. DEVICE DESCRIPTION

Standard version of the Photobioreactor FMT150 consists of a cultivation vessel with a sealable lid and a base box

containing electronics circuitry, LED light panel (Fig. 1A) and other components essential for the optimal operation of

the photobioreactor.

Page 6

Fig. 1: A) Photobioreactor body consists of a flat vessel holding the suspension and of a base box with electronics circuitry. B) The

block scheme of the instrument shows the Control Processor Unit exchanging data and protocol with external computer (PC) and

auxiliary external modules. The Control Unit governs measurements of parameters such as OD and chlorophyll fluorescence plus

parameters measured by auxiliary sensors. It also controls the actinic light sources and temperature of the culture. C) Detail of the

LED configuration. D) Uncorrected optical density spectrum of cyanobacterial suspension. The lines with arrows inate wavelengths of

various light sources that are integrated in the instrument. The detection range of the photodiode is also shown. E) Detail (rotated

top-to-bottom view) of the fluorometer and densitometer. F) Schematic representation of optional gas supply components and

modules that can be used to stabilize aeration and set CO concentration level.

3.Device Description

The array of high-power light emitting diodes (LEDs) is located behind the cultivation vessel.

In the bottom corner of the front of the vessel is a semiconductor light sensor for measuring fluorescence emission

and suspension optical density by attenuation of light that was emitted from the LEDs (Fig. 1E)

The Photobioreactor can also control external modules such as peristaltic pumps and solenoid valves via the

photobioreactor control unit (shown schematically in Fig. 1F and Fig. ). The solenoid valves are used to switch off the

gas supply to the culture (bubbling) during optical measurements. Peristaltic pumps are used in turbidostat and

chemostat modes to supply fresh medium or buffer.

Page 7

Fig. : Schematic diagram of the Photobioreactor, its modules, and remote control.

3.Device Description

3.1 CONFIGURATIONS AND VERSIONS

FMT150 can be shipped with various configurations of vessel capacity, light quality, light intensity and

thermoregulation. Numerous additional accessories such as various sensors can be supplied as described in chapter 5

on page 30.

Please note that this manual describes operation of two FMT150 versions (Fig. 4) - older PBR FMT150.1 and new PBR

FMT150. .

3.1.1 CULTIVATION VESSEL

The cultivation vessel is flat and rectangular in shape. Its front and back windows are made of glass plates. The base is

made out of stainless steel and contains a thermal bridge that facilitates heat transfer between a Peltier cell in the

instrument base and the culture suspension. The lid of the vessel is easily detachable during cleaning and contains

Luer connectors for gas or medium supply tubing, waste medium exhaust or sample collection. The three larger ports

accommodate the optional temperature/pH sensor and dissolved O or CO probes.

The Photobioreactor FMT150 is manufactured in three vessel capacities enabling the customer to select optimal

cultivation conditions for his experiments.

•400 ml (in FMT150/400)

•1000 ml (in FMT150/1000)

•3000 ml (in FMT150/3000)

3.1. LED LIGHTING

The high-power light emitting diodes generate a highly uniform irradiance flux that can be controlled in the range

0-3000 mol(photons)/m /s PAR. The irradiance can be dynamically modulated by the FMT150 control unit or by the

PBR software. Spectra are shown in Fig. 3.

Two s andard LED panel versions are available, each color is controlled independently:

•White-Red panel: maximum intensity 1,500 µmol(photon)/m /s (white 750 / red 750 µmol(photon)/m /s)

•Blue-Red panel: maximum intensity 1,500 µmol(photon)/m /s (blue 750 / red 750 µmol(photon)/m /s)

Two optional upgraded LED panel versions are available, each color is controlled independently:

•White-Red panel: maximum intensity 3,000 µmol(photon)/m /s (white 1500 / red 1500 µmol(photon)/m /s)

•Blue-Red panel: maximum intensity 3,000 µmol(photon)/m /s (blue 1500 / red 1500 µmol(photon)/m /s)

Optional cus omized panel versions:

•White-Blue: maximum intensity 1,500 µmol(photon)/m /s

•White-Blue with Light Upgrade: maximum intensity 3,000 µmol(photon)/m /s

•Red: maximum intensity 750 µmol(photon)/m /s (no light upgrade available)

•White: maximum intensity 1,500 µmol(photon)/m /s

Page 8

3.Device Description

•White with Light Upgrade: maximum intensity 3,000 µmol(photon)/m /s

•Blue: maximum intensity 1,500 µmol(photon)/m /s

•Blue with Light Upgrade: maximum intensity 3,000 µmol(photon)/m /s

3.1.3 THERMOREGULATION

It requires presence of the pH/Temperature probe.

•Standard range of 18 – 50 °C (static or ramping).

Please find approximate estimation of time required for the given temperature equilibration in the Table 1 below.

Initial

temperature

Target

temperature

Light conditions

µmol/m /s

Equilibration time

required

5 °C 50 °C 1500 .5 hours

50 °C 5 °C 1500 3 hours

Table 1: Tempera ure equilibra ion imes

•Optional enhanced range 0 – 70 °C (static or ramping) (requires enhanced vessel made from nickel-plated

brass covered with ceramic layer).

3.1.4 VERSIONS

The available vessel capacity for two FMT150 versions:

•FMT150.1 – 400 ml, 1000 ml

•FMT150. – 400 ml, 1000 ml, 3000 ml

Page 9

Fig. 3 LED spectra of PBR FMT150

3.Device Description

Impor an : The FMT150 in both 400 and 1000 ml models has been upgraded from FMT150.1 (Fig. 4A) to FMT150.

(Fig. 4B). This manual provides instructions for operation of both versions (original, now referred to FMT150.1 and the

current version FMT150. ). Please always ensure that you refer to correct instructions.

List of parameters measured and/or calculated by the Photobioreactor FMT150

Page 10

Fig. 4: Two versions of the PBR FMT 150: A) FMT 150.1, B) FMT 150. .

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