PerkinElmer UATR Manual de usuario

UNIVERSAL ATR SAMPLING ACCESSORY

User’s Guide

MOLECULAR SPECTROSCOPY

Release History

Part Number Release Publication Date

L1050231 C September 2017

Any comments about the documentation for this product should be addressed to:

User Assistance

PerkinElmer, Inc.

710 Bridgeport Avenue

Shelton, CT 06484

Notices

The information contained in this document is subject to change without notice.

Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of

any kind with regard to this document, including, but not limited to, the implied warranties of

merchantability and fitness for a particular purpose.

PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in

connection with furnishing, performance or use of this material.

This document contains proprietary information that is protected by copyright.

All rights are reserved. No part of this publication may be reproduced in any form whatsoever or

translated into any language without the prior, written permission of PerkinElmer, Inc.

Trademarks

Registered names, trademarks, etc. used in this document, even when not specifically marked as such,

are protected by law.

PerkinElmer is a registered trademark of PerkinElmer, Inc.

Spectrum and Spectrum Two are trademarks of PerkinElmer, Inc.

Contents

Conventions Used in this Manual........................................................................ 4

Notes, Cautions and Warnings..................................................................... 4

Introduction...................................................................................................... 5

Unpacking the UATR ......................................................................................... 7

Installing the UATR ........................................................................................... 8

Fitting the accessory................................................................................... 8

Accessory detection by Spectrum software..................................................10

Taking a Reference Background spectrum...................................................10

Contamination Check........................................................................................11

Cleaning the crystal ...................................................................................11

Performing a Contamination Ready Check...................................................11

Performing Ready Checks that use Polystyrene ..................................................12

Handling Samples ............................................................................................13

Solid samples ............................................................................................13

Liquid samples ..........................................................................................13

Obtaining a Sample Spectrum with Spectrum Software.......................................14

Obtaining a spectrum of a solid sample, using the pressure arm...................14

Obtaining a spectrum of a liquid sample......................................................17

Maintenance ....................................................................................................19

Replacing the pressure arm o-ring ..............................................................19

Replacing the top-plate..............................................................................19

4 . Universal ATR Sampling Accessory User’s Guide

Conventions Used in this Manual

Normal text is used to provide information and instructions.

Bold text refers to text that is displayed on the screen.

UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is

used to show that you have to press two keys at the same time, for example, ALT+F.

All eight digit numbers are PerkinElmer part numbers unless stated otherwise.

The term ‘instrument’ refers to a Spectrum Two Series spectrometer, and any sampling

accessory fitted.

Notes, Cautions and Warnings

Three terms, in the following standard formats, are also used to highlight special

circumstances and warnings.

NOTE: A note indicates additional, significant information that is provided with some

procedures.

CAUTION

ATTENTION

We use the term CAUTION to inform you about situations that could

result in

serious damage

to the instrument

or other equipment.

Details about these circumstances are in a box like this one.

Nous utilisons le terme «ATTENTION» pour vous informer des situations

pouvant entraîner des dommages sérieux à l'instrument ou à d'autres

équipements. Les détails sur ces circonstances se trouvent dans une boîte

comme celle-ci.

WARNING

AVERTISSEMENT

We use the term WARNING to inform you about situations that could

result in personal injury to yourself or other persons. Details about

these circumstances are in a box like this one.

Nous utilisons le terme AVERTISSEMENT pour vous informer des

situations pouvant entraîner des blessures personnelles pour vous-

même ou pour d'autres personnes. Les détails sur ces circonstances se

trouvent dans une boîte comme celle-ci

Universal ATR Sampling Accessory User’s Guide . 5

Introduction

The Universal Attenuated Total Reflectance Accessory (UATR) is an internal reflection

accessory, used with a Spectrum Two Series spectrometer, for simplifying the analysis of

solids, powders, pastes, gels and liquids. The technique is non-destructive.

As the beam does not penetrate deeply into the sample, this technique is ideal for analyzing

strong infrared absorbing solutions, such as emulsions or aqueous solutions. The technique

can also prove useful in measuring homogeneous solid samples, solid surfaces and coatings

on solid samples.

Figure 1 The Universal ATR Sampling Accessory

6 . Universal ATR Sampling Accessory User’s Guide

How the UATR works

The technique involves placing a sample on top of a crystal with a high refractive index. An

infrared beam from the instrument is passed into the accessory and up into the crystal. It is

then reflected internally in the crystal, and back towards the detector which is housed in the

instrument. When the beam is reflected within the crystal, it penetrates into the sample by a

few microns. Figure 2 illustrates this process.

Figure 2 Principle of UATR operation

The UATR can be used to analyze homogenous solid samples, solid surfaces, or coatings on

solid samples. Force may be applied to ensure good contact.

The Spectrum Two UATR uses a diamond crystal. The diamond crystal is hard, is not easily

scratched, is resistant to strong acids and bases, and can withstand high pressures. The

diamond UATR has an effective scanning range that matches the full range of the

instrument, although sensitivity is somewhat reduced in the approximate range 1900–

2700 cm−1.

Universal ATR Sampling Accessory User’s Guide . 7

Unpacking the UATR

As soon as you receive your UATR Accessory, make sure that it includes the parts listed

below. If anything is missing or damaged, contact your local PerkinElmer office.

The UATR Accessory itself is shipped fully assembled and ready to install.

Part Number Description Quantity

UATR Accessory 1

L1600239 Test Sample Holder 1

L1601742 Medium Shoe 1

L1601743 Large Shoe 1

L1601746 Volatiles Cup 1

L1601856 Apron 1

L1601857 Transit Shoe 1

L1601862 Transit Friction Pad 1

L1050242 Spectrum Two Manuals CD 1

L1100667 UATR Accessory Shipping Disk 1

The Shipping Disk contains reference spectra for your accessory which can be used to check

if its performance has changed.

8 . Universal ATR Sampling Accessory User’s Guide

Installing the UATR

Fitting the accessory

The UATR fits into the sample compartment of the spectrometer, replacing the sample plate.

NOTE: Before you begin you should, if necessary, loosen the pressure arm and release the

transit block that secures the UATR pressure arm during transport.

You may also need to remove the covers on the left and right sides of the accessory,

and the top-plate dust cover.

Store all these in a safe place for future use, if required.

To remove the sample plate and install the UATR:

1. Raise the sample cover of the spectrometer to the vertical position and lift it upwards,

clear of the instrument.

Store it in a safe place for future re-use.

2. Slide the sample plate out of the sample area (Figure 3).

Store it in a safe place for future re-use.

Figure 3 Removing the basic sample plate

Universal ATR Sampling Accessory User’s Guide . 9

3. Pivot the UATR on the ledge at the front of the sample area (Figure 4a), then tip it

backwards (Figure 4b).

NOTE: If the accessory is pivoted too far forward on the sample area ledge it will not move

correctly into position.

(a)

(b)

Figure 4 Installing the accessory

4. Push the UATR home firmly, ensuring that the top of the accessory mates properly with

the lid of the instrument (Figure 5).

The UATR is now ready for use.

Figure 5 UATR installed in a Spectrum Two

10 . Universal ATR Sampling Accessory User’s Guide

NOTE: When the UATR is installed, a connection is made between the accessory and the

purge system used in the instrument. To purge the accessory, connect to the white

plastic purge connector on the underside of the instrument, at the right.

Accessory detection by Spectrum software

The Spectrum software detects the presence of the accessory automatically, and the Setup

Instrument Basic (Figure 6) and Setup Instrument BeamPath (Figure 7) tabs are updated to

show details of the UATR accessory.

Figure 6 Setup Instrument Basic tab with UATR details circled

Figure 7 Setup Instrument BeamPath tab with UATR icon circled

Taking a Reference Background spectrum

Once you have installed your UATR, and before you use it for the first time, you should

consider taking a background spectrum which will act as a reference background for use with

those Ready Checks that need one for comparison purposes, including the

Contamination

Check

described on page 11.

NOTE: For information about taking a background spectrum, see the Spectrum on-screen

Help.

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