Olympus BX53 Manual de usuario



A.S

1. Si

2. T

3. T

4. S



B.H

1. Li

2. S

3. H

4. P

WMicrosco

stemStart‐

gnintothel

rnon(orw

rnonthem

nonlamp



artcellSens

rdwareCon

htpathbar

lectanobje

logenlamp

htwillbevi

iorZ‐stage

osetotheo

ob;theexte

eselecteds

ttononthe

c

e

j

yLaborator

Oly

p.

gbookont

keup)thec

croscope

(onlyifyou

imension

rolandSett

directsli

tivelensby

dialknob

iblefromth

ontrolBox

jectiveand

ntofthefin

eedappear

controller.)

d



pus B

edesk(star

mputerand

themainco

eeditforflu

program.

ng.

httoeyepie

urningthen

ontrolsthe

condenserl

movesthe

OWNbutto

movement

ontheLED

53 Digi

/endtime).

select“USER

”

trolunit

,

orescencei

e,camera,

sepiecetur

rightnessof

ns.

tageupord

bringsitdo

anbeselect

creen(Note

f

al Micr

”

andentert

hePriorre

aging).

rboth.

byhan

transmissio

owntofocus

nquickly.

dHigh(H),

DON’TUSE

c

Oly

scope

hepassword

otez‐stage

.

lightforbri

onspecime

inermovem

edium(M),

hefocuskn

h



i

mpusB

User G

onthelog‐i

ontroller

,

htfieldview

.TheUPbu

ntofstage

orlow(L)by

bonthemi

c

53User

ide

window.

DP26colorc

ing.Thetra

tonbringst

anbecontr

pressing“SP

roscopean

g

Guide|

mera

,an

smission

estageup

lledwiththe

EED”button.

theZERO



5. R



C.O

WMicrosco

motecontr

ttonstosel

enorclose

erviewofc

henthepr

istochemica

indowtab

electanobj

tagewilllow

atchingobj

bjectivetoo

lickoneof

ocusontoth

anel.

1)

1!

yLaborator

llerbox

c

ctanappro

hemercury

llSenseprog

gramopens,

lystainedsa

.

ctive(start

erandames

ctiveinplac

btaincorrect

esampleby



ntrolsthes

riatefilterm

rclampligh

amandbas

firstsetupt

plesorflu

ith5xor10

sagewindo

.ClickOKo

scaleba

).

butto

movingstag

lectionofi

irrorcubefo

shutter.

cimageacq

eprogramf

rescenceim

objective)b

willpopup.

nthemessa

sontheOb

withPriorz

‐

agingmode

(

differentco

isition.

oryourimag

gingforfluo

pressingan

Turntheob

ewindow(I

ervationMe

‐

stagecontr

Oly

(i.e.BForFlu

lorsoffluore

ingneeds(i.

rescentlylab

objective

ectivenose

iscriticalto

thodspanel

llerorbycli

mpusB

orescence(F

scence.Pre

.bright‐field

ledsamples

ieceturret

atchthem

orbrightfiel

kingarrowh

53User

L)).Usethe

stheFLShu

imagingfor

).Clickthe

byhandto

gnification

orfluoresc

adsintheD

Guide|

irror

terbuttont

cquisition



button.Th

utthe

iththe

nceviewing.

viceUnits



(



D.B

(



WMicrosco

electacam

rightfield.

utomaticfr

electResolu

(

active)scen

rightfield(o

akesureth

heupperlig

lickLive

Automatice

orkfinefor

hentheset

s.vsior.tiff

yLaborator

rafromthe

electManu

mthewhite

ion

asde

.)

DIC)Image

tDP26cam

tpathbar

button

posure

w

mostcases).

ingissatisfa

rmatbyclic



ameralist

)

lorAutoma

balancemo

ired.(Note:

Acquisition

raisONand

ispushedin

andobtain

ithSpot30%

Clickthebu

ctory,clickS

kingtherigh

)

:XM10mon

ticexposure

elist.

oucanchoo

selectedfro

andthelow

roperexpos

regionanda

tonagainto

ap

mousebutt

chromeca

toobtainbe

ealowerre

thecamer

rbar1

is

reandwhit

utomaticw

stoplivevie

buttonands

noverthei

Oly

eraforfluo

terimages.

olutionforL

listandtha

ulledout.

balance

itebalance

ing.

avetheima

agetab.

mpusB

escenceor

ithDP26c

ive/Movie

t



e

53User

P26colorca

meraselect

rfas

updat

1@

1#

Guide|

erafor

d,select

oflive



E.Fl

1.

2.

one

posi

and

WMicrosco

uorescenceI

licktheFLb

noroffbyp

electanapp

ftheObser

ion

.Mak

helowerba

d

yLaborator

mageAcqui

tton

ont

ressingFLSh

ropriatefluo

ationMeth

surethatth

pushedin)



ition

eremotec

utterbutton



escencemir

dsbuttons

lightpath

ndXM10ca

ntrollerbox

onthere

orcubebyp

ars

arein

eraisselec

ousemercu

otebox.

ressingMirr

(exce

correctposi

ed.

Oly

ryarclampl

rbuttons

tBFandDIC

ion(theupp

e

mpusB

ghtforfluor

inthecontr

).Theselect

rbaratthe

53User

scence.Th

llerboxorcl

edmirrorwil

midorexten

f

Guide|

lightcanbe



ickingany

lbein

dedposition

b



3.L

and



4. Cl



5. Cl



6. I



7. S



WMicrosco

t’stakemul

xRedfluoro

icktheProc

ulticolorwh

elist.Repe

xttotheca

tton.IfAut

posuretime

nditionsfor

icktheFITC

timalexpos

annel.Once

reachchan

.vsifileint

vetheorigi

agescanals

changethe

oneimage

vetheimag

alebarcan

splayedwith

age.Savet

yLaborator

icolorfluore

hores.

ssManager

el butto

ttwicetose

eraicon;it

maticexpos

.Oncethee

DAPI.

ectiononth

retimeand

allthesettin

elautomatic

eLibraries>

alimagefile

obesaveda

mageinto8

‐

ontainingm

asmultiple

eviewedwi

View/InfoS

eannotated



cenceimag

andch

.Clickthe

ectFITC(gre

illexpandt

ure(withSp

posurecon

listanduse

clickico

sareobtain

ally.Theres

icturesfold

as.vsiform

.tifffiles.S

‐

bitimage.U

ltiplechann

magesfore

hView/

cal

ampcomm

imageasJP

acquisition

ecktheAuto

en)andTxRe

showcurre

t30%)isus

itionsarese

theAutoma

toreadthe

ed,clickthe

ltingmultic

rassoonas

twithouta

lectImage>

eFile>Save

ls.OruseFi

chchannel.

barcomma

nd.Thesea

G(JFIF)(*.jpg

ithanexam

maticProce

butto

d(red)chan

texposure

d,letthepr

,clickic

icexposure.

setting.Rep

but

annelimage

heacquisiti

ymanipulati

ode>8bit/

s…menuco

e>Exportto

ndandthe

notationsc

).

Oly

plestained

ses

.Click

andselect

els.Clickth

etting.Click

gramfindth

ontosetthe

Waituntilit

eatoncemo

on.Itwillta

,bydefault,

nhasbeenc

onorproces

hannelmen

mmandtos

tiffseries…

agnification

n’tbesaved

mpusB

ithDAPI,FI

the

API(blue)in

“plus”sign

theLive

eappropriat

seexposure

indsan

eforTxRED

kethreesho

illbesaved

ompleted.

ing.

ucommand

vetheimag

ommandto

infocanbe

withyourtif

53User

C,



s



Guide|

g



F.I

1. O



2. I



WMicrosco

ageProces

nceyousave

publication.

stogramand

default,the

mensionSel

teyesymb

wantedcha

yLaborator

ing

theoriginal

ClickthePr

thebrightne

adjustment

ectorpanel

lindicates“i

nels.Then,



ile,makea

cessingwin

ssandcontr

ftheHistog

,youcanch

visible”).Cl

youcanadju

uplicateifit

owtab

stofthedis

amortheDi

oosethecha

ckthecolor

stthehistog

snecessary

fro

laycanbea

splayEnhan

nnelstobev

hannelsyou

amandthe

Oly

omakeany

themainwi

justed.

ementappli

isible(eyesy

wanttosho

rightness/c

mpusB

hangesinth

ndowtabs,i

estoallthe

mbolind

andclicko

ntrastofth

53User

eimagefor

whichthei

isiblechann

cates“visibl

ntheeyesy

selectedch

Guide|

resentation

age

ls.Onthe

”andgrey‐

bolstohide

nnelonly.



3. 



(



WMicrosco

osavesome

erationswil

1) Makeac

Edit/Pas



2) Makeac

Thiswill



3) Selectall

multicha

selectch

c

yLaborator

overlaidima

automatica

mpositeim

eAs/NewI

mpositeim

reateanew

channelsfro

nelimagein

nnelstobe



esfromam

lychangeth

geasabove.

age.Thiso

geasabove.

magewiths

theDimen

oindividual

ombinedan

ultichanneli

imagetype

FromtheEd

erationwill

UseEdit/Co

alebarinpi

ionSelecto

channelima

clickOK.

ageyoujus

rom16bitg

itmenu,use

enerateaco

yDisplayto

elunit(losin

.UseImage

es.Thenus



Oly

madeasab

rayscaleto8

Edit/Select

mpositeima

Clipboar

a

gsomedime

Separate/C

Image/Co

mpusB

ve,therear

bitcolo

):

ll,thenEdit

ewiththes

dthenEdit

nsionalinfor

annelscom

bineChann

53User

ethreeways

Cop

,andt

mescaleba

PasteAs/Ne

mation).

andtosep

.Inthe

Guide|

(note:these

en

r.

Image.

ratethe

indow,



F.A

1. A

2. T

It

3. Cl



G.D

1. I

3. Y

4. Y

WMicrosco

quisitionof

z‐stackimag

ackbu

ovethestag

ocessMana

osetotheco

ingupthest

tton

,whi

sition.)

eprogram

jective.Clic

willcalculat

icktheStart

splayingan

theProcess

iththenavi

amesofaz‐s

ucanselect

ousebutton

amefromth

ucanalsos

ttons

;de

artingPositi

enchoose

icktheright‐

ntainingonl

coverslip

Slide

yLaborator

‐stack(z‐ser

containsm

tontodispla

usingtheP

erwindow.

verslipand

agetofocus

hwilltheSt

illautomati

kApplybutt

thenumber

buttontoca

Converting

ngtab,thez

‐

atorbar

tack.

asinglefra

inasinglefr

econtextm

lectashort

ermineane

nandchoo

leView.M

ousebutto

selectedfr



ies)

ltipleframe

Zstackpan

iorstageco

First,lower

henclickSet

ontoadeep

rtposition.

(

allyrecomm

ntoaccept

ofz‐slices



tureaz‐seri

‐stacks

‐

stackimage

efromaz‐st

meviewan

nu.

angeoffra

startingpo

eanewend

kesureallt

andselect

mewillbeg

Objectiv

acquiredat

el.Tosetth

trollerorby

hestagetof

button

,w

rregionclos

(

Imageacqui

ndastepsi

hisstepsize

accordingly.

s.Youcanc

canbemani

ackbyclicki

selectingE

eswithinth

itionwithin

positionand

echannelsa

xtrac

from

neratedas



differentfoc

StartandE

clickingthe

cusontoar

ichwillbet

totheslide

itionwillbe

e

forz‐se

orchangeas



mbinethis

ulatedinva

gtheright‐

tractCurren

stackbyusi

hestackan

clickSet

evisible(sel

hecontext

.vsifile.Sav

Oly

uspositions.

dlimitsofa

rrowheads

egionofinte

eEndpositi

andclickSe

inattheSta

iesaccordin

youwantan

unctionwith

iouswaysto



ngSetSelect

click

etSel

ctionEndP

ected).Over

enu.Ane

theimage.

End

Start

onstag

mpusB

Clickz‐

z‐stack,

inthe

rest

on.Next,



t



tothe

dapply.

multicolori

generatedi

,youc

ion

ction

sition.

animage,

z‐stack

g

53User

aging.Sav

ferentviews

nnavigated



f

Guide|

theimages.



ifferentfocal

b

c

d



5. Y







WMicrosco

ucanproje

omthemain

iththe

lice

ywhereby

iththeslice

mensionvie

yLaborator

tallthez‐st

menuorM

Viewmenu,

ovingthex

iewmenuo

ofextende



ckframesin

imumProj

oucandispl

cross‐hairlin

tions

x‐yslicepo

oasingle2

ctionOverZ

ayacross‐se

etothedesi

ition.

imagebyse

onthenavig

tionalz‐dim

edposition.

,suchas

Oly

ectingProce

torbarmen

ensionview



ExtendedSli

mpusB

ss/Projectio

u.

fanxandy

ce ,

53User

s/Maximu

plane.Youc

youcanvisu

Guide|

Zcommand

nview

alizez



Otros manuales para BX53

Otros manuales de Microscopio de Olympus

Olympus

Olympus CX40 Manual de usuario

Olympus

Olympus Fluoview FV1000 Manual de usuario

Olympus

Olympus Fluoview FV1000 Manual

Olympus

Olympus VANOX Manual de usuario

Olympus

Olympus DP73 Manual de usuario

Olympus

Olympus SZX16 Manual de usuario

Olympus

Olympus IX71 Manual de usuario

Olympus

Olympus U-D7RES Manual de usuario

Olympus

Olympus Pom Manual de usuario

Olympus

Olympus AL120-12 Series Manual de usuario

Olympus

Olympus BX51 Manual de usuario

Olympus

Olympus K Instrucciones de funcionamiento

Olympus

Olympus BH2 Series Instrucciones de funcionamiento

Olympus

Olympus CHD Manual de usuario

Olympus

Olympus CX23 Manual de usuario

Olympus

Olympus CX21 Manual de usuario

Olympus

Olympus CHBS Manual de usuario

Olympus

Olympus VM Series Manual de usuario

Olympus

Olympus BX60 Manual de usuario

Olympus

Olympus CKX53 Manual de usuario

Olympus

Olympus DP21-SAL Guía del usuario

Olympus

Olympus BHT Manual de usuario

Olympus

Olympus BX46 Manual de usuario

Olympus

Olympus CK40-TBI Instrucciones de funcionamiento

Manuales populares de Microscopio de otras marcas

Thermo Scientific

Thermo Scientific Nicolet iN10 Guía

National

National DC3-163 Manual de usuario

KERN Optics

KERN Optics OPE-1 Manual de usuario

OPTIKA MICROSCOPES

OPTIKA MICROSCOPES B-150D Manual de usuario

Prior

Prior Lux POL Manual de usuario

Opitec

Opitec Smartscoop 115615 Manual de usuario

KERN

KERN OPO-1 Manual de usuario

Topcon

Topcon SP-1P Manual de usuario

Motic

Motic PA43 Manual de usuario

Leica

Leica DVM5000 Guía del usuario

MshOt

MshOt MZ62 Manual de usuario

KERN Optics

KERN Optics OZR-5 Manual de usuario

VWR

VWR VisiScope 384 Series Manual de usuario

Nikon

Nikon ECLIPSE E200 POL Manual de usuario

Leica

Leica DI C800 Manual

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manual de usuario

ThermoFisher Scientific

ThermoFisher Scientific Continuµm Manual de usuario

Leica

Leica FL560 Manual de usuario