OHAUS ACROSS PRO AO-2 Manual de usuario

ACROSS PRO

Digital Adjustable

Manual Microliter Pipette

Micro Pipeta de Volumen Variable

Réglable numériquement

Manuel de la pipette Microliter

AO-2

AO-10

AO-20

AO-100

AO-200

AO-1000

AO-5000

AO-10ML

AO8-200

9 models for volume ranges from 0.1 μL to 10 mL

9 modelos para rangos de volumen de 0.1 μl a 10 ml

9 modèles pour des gammes de volumes de 0,1 µl à10 ml

ACROSS PRO

Digital Adjustable Manual Pipettes

CONTENTS

Description………………...……………………………………………………....1

Contents………………………..………………………………………………….2

Autoclaving…………………………..…………………………………………....2

Volume Indicator...........................................................................................2

Safety Filter, AO-5000 & AO-10ML...............................................................3

Operation......................................................................................................3

Tips Selection................................................................................................5

Tips Immersion Depth...................................................................................5

Pipetting Guidelines & Precautions...............................................................6

Pre-Rinsing Recommended..........................................................................6

Reverse Mode Pipetting................................................................................6

Pipetting Liquids of Varying Density..............................................................7

Temperature Considerations.........................................................................7

Acids and Corrosives....................................................................................7

Storage..........................................................................................................8

Maintenance………………………...…………................................................8

Calibration.....................................................................................................8

Warranty .......................................................................................................9

Removing/Replacing Tip Ejector Arm...........................................................9

TipEjector.................................................................................................. ...9

Performance Specifications........................................................................10

Replacement Parts / Accessories……………..………………………………11

FIGURES

Figure 1 ACROSS PRO AO-200……...………………….……………….……..1

Figure 2 Operating ACROSS PRO ……………..……………………………..4

Figure 3 Pipette Stand…………………….………..........................................8

Figure 4 Removing Tip Ejector Arm…………………………………………….9

Figure 5: Cam-actuated Tip Ejector…………………………………………. 10

DIAGRAMS FOR REPLACEMENT PARTS

Models AO-2, AO-10, AO-20, AO-100…….................................................12

Models AO-200, AO-1000, AO-5000, AO-10ML, AO8-200....................13 14

TABLES

Specifications…….......................................................................................10

Prices and specifications are subject to change without notice.

Digital volume indicator

Coupling nut

Shaft

Friction Ring/

Volume Adjustment

Precision Micrometer

Description

Nine models of ACROSS PRO continuously adjustable digital pipette cover

the entire volume range from 0.1μl to 10ml.

Piston stroke is set by adjusting a micrometer coupled directly to a digital

volume indicator, which reads in microliters.

All Ohaus ACROSS PRO models have a highly polished stainless steel

piston, polyethylene/Teflon seal and o-ring (except AO-10ML, which uses a

grease seal). ACROSS PRO requires no routine lubrication and will give

years of trouble-free service.

A plastic tip ejector is provided with all models (except AO-5000 and AO-

10ML) for safe disposal of used tips.

Figure 1. ACROSS PRO AO-200

Plunger Button/

volume adjustment

Tip ejector button

(except AO-5000

and AO-10ML)

Tip ejector arm

(except AO-5000

and AO-10ML)

High-impact,

low thermal

conductivity

body

Contents

ACROSS PRO Sample tips

Instruction Manual Test report / Warranty Card

Autoclaving

The Shaft and tip ejector are autoclavable: 121℃, 1 atm, 15-20 minutes).

(AO-5000, AO-10ML do not use tip ejectors.)

Volume Indicator

The volume indicator is read from top to bottom. Up to AO-200 black digits

indicate microliters and red digits tenths and hundredths of microliters. For

AO-1000 and AO-5000，red digits indicate milliliters and black digits

microliters. For AO-10ML, black digits indicate milliliters and red digit tenths

of a milliliter.

AO-2 AO-10 AO-20 AO-100 AO-200 AO-1000 AO-5000 AO-10ML

1.25 µL 7.5 µL 12.5 µL 75 µL 125 µL 750 µL 1.25 mL 7.5 mL

Sample values, volume ranges, and smallest increments for each ACROSS

PRO model are shown below:

Model Range (μL) Recommended (μL) Min. Increment (μL)

AO-2 0 to 2 0.1 to 2 0.002

AO-10 0 to 10 0.5 to 10 0.02

AO-20 0 to 20 2 to 20 0.02

AO-100 0 to 100 10 to 100 0.2

AO-200 0 to 200 20 to 200 0.2

AO-1000 0 to 1000 100 to 1000 2.0

AO-5000 0 to 5000 500 to 5000 2.0

AO-10ML 0 to 10mL 1mL to 10mL 20.0

AO8-200 0 to 200 20 to 200 0.2

Black Digits

Red Digits

Safety Filter, AO-5000 and AO-10ML

Models AO-5000 and AO-10ML use a safety filter in the shaft to help

prevent liquid entering the shaft and contacting the piston, if the plunger

snaps up during aspiration. This is particularly important using large

volumes. If the filter gets wet, replace it.

For AO-5000 insert the small diameter into the shaft, for AO-10ML insert

the large diameter into the shaft. Part number: 30079146 (pack of 100).

Operation

1. Turn the plunger button or the volume adjustment knob until the

volume indicator is 1/3 revolution above the desired setting, then turn

slowly clockwise until the desired volume shows on the indicator.

2. ALWAYS dial down to the desired volume. This prevents mechanical

backlash from affecting accuracy. If you pass the desired setting, turn

the dial 1/3 revolution higher than desired and dial down to reset the

volume. The friction ring prevents unintentional volume changes.

3. Attach a new disposable tip to the pipette shaft. Press into the volume

displayed on the indicator.

4. Holding Across PRO vertically, immerse the tip into the sample to

the proper depth; see table on page 5.

5 Allow the pushbutton to return slowly to the UP postion. Never let it

snap up! See figure 2A.

6. Pause briefly to ensure that the full volume of sample is drawn into

the tip.

END OF

5000 µL SHAFT

END OF

10 ML SHAFT

7. Withdraw the tip from the sample liquid. If any liquid remains on the

outside of the tip, wipe it carefully with a lint-free tissue, avoiding the

tip orifice.

8. To dispense sample, touch the tip end against the side wall of the

receiving vessel and depress the plunger slowly to the first stop. See

Figure 2B.

Wait 1 second: AO-2 ~ AO-200

1-2 secs: AO-1000

2-3 secs: AO-5000, AO-10ML

(Longer for viscous solutions).

Then press the plunger to the SECOND STOP (bottom of stroke),

expelling any residual liquid in the tip. See Figure 2C.

9. With the plunger fully pressed, withdraw the pipette from the vessel

carefully, with the tip against the vessel wall.

10. Allow the plunger to return to the up position.

11. Discard the tip by depressing the tip ejector button. A fresh tip should

be used for each sample to prevent sample carryover.

Figure 2. Operating ACROSS PRO

TOP OF STROKE

1st STOP

2nd STOP

Tip Selection

Tips must seal properly on the shaft to assure an air-tight seal and

avoid leaks or poor accuracy.

Tips must be soft and flexible so that the shaft is not scratched or

worn prematurely.

The tip orifice must be the correct size, and orifice size and

geometry must be consistent from tip to tip. Otherwise, accuracy

and precision will be affected.

Interior and exterior surfaces must be clear, smooth, and

hydrophobic to avoid retention of liquid. Too much retention results

in poor accuracy and reducibility.

Specified performance is guaranteed only when OHAUS disposable

tips are used as recommended in this manual.

OHAUS cannot accept responsibility for poor performance resulting from

the use of tips by other manufacturers. OHAUS tips are molded from

premium-grade virgin polypropylene plastic. Samples from each lot of tips

are inspected microscopically to ensure that every lot meets OHAUS’s high

standards.

Tip Immersion Depth

The recommended depth for tip insertion into the sample for each ACROSS

PRO model is shown in the table below:

Model Immersion Depth

2 - 10 μL 1 –2 mm

20 - 100 μL 2 –3 mm

200 - 1000 μL 3 –6 mm

5000μL –10 ml 6 - 10 mm

Tip immersion depth is important. If exceeded, the volume measured will be

inaccurate, possibly out of specification.

Tip angle is also important. Hold the pipette vertically, or within 20 degrees

of vertical.

Pipetting Guidelines＆Precautions

Consistency in all aspects of pipetting will significantly contribute to

optimum reproducibility.

1. Consistent pickup/dispense rhythm while pipetting.

2. Consistent speed and smoothness when you press and release the

pushbutton.

3. Consistent pushbutton pressure at the first stop.

4. Consistent immersion depth.

5. Minimal angle (＜20°from vertical)

6. Never invert or lay the pipette down if liquid is in the tip.

Pre-Rinsing Recommended

Some solutions (e.g. serum, protein-containing solutions, and organic

solvents) can leave a film on the inside tip wall, resulting in an error larger

than the tolerance specified. Since this film remains relatively constant in

successive pipetting with the same tip, excellent precision may be obtained

by refilling the tip and using the refilled volume as the sample. Successive

samples from this same tip will exhibit good reproducibility.

Reverse Mode Pipetting

Reverse mode pipetting is recommended for highly viscous, biological or

foaming liquids, or very small volumes of liquid.

1. Mount a disposable tip on the pipette shaft.

2. Press the pushbutton fully to the second stop.

3. Immerse the tip in liquid and return the plunger slowly to the full up

position. Wait a moment for the liquid column to reach equilibrium in the

tip.

4. Wipe any excess liquid from outside of the tip without

touching the orifice.

5. To dispense, rest the end of the tip against the vessel

wall and press the plunger to the first stop only. Hold at

the first stop for a few seconds-long enough for the liquid

column to reach equilibrium again.

6. Remove the tip from the receiving vessel without blowing

out the remaining liquid.

7. Return excess sample in the tip to the original sample

container, if desired. Discard the used tip.

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