NanoEnTek ADAM-MC Manual de usuario
A New Standard of
Automatic Cell Counter
ADAM
INSTRUCTION MANUAL
NESMU-AMC-001E (V.5.5)
A New Standard of
Automatic Cell Counter
ADAM-
MC
USER MANUAL
NESMU-AMC-001E (V.5.1)
2
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Table of Contents
Product Contents
Safety Information
Product Specifications
Description of ADAM-MC
3
4 ~ 6
7
8 ~ 9
Introduction
Overview
Basic principals of counting
10 ~ 11
10
11
Getting Started
Environmental requirements
Installation
Start-up screen
Error message during booting
Menu setting
System information
Chip selection
Counting method
12 ~ 18
12
13
14
15
16
16
17
18
General Operation
Introduction
Preparing cell
Counting cell
Operating ADAM-MC
Icon function
Result analysis
Error code
Maintenance and cleaning
19 ~ 24
19
19
19
20
21
22
23
24
Software Installation
Introduction
Getting started
Installation
Function guide
Function buttons
Data list
Information
Graph
Sample image of error message
25 ~ 32
25
25
26 ~ 27
28
29
30
30
31
32
Trouble shooting
Warranty
Product list
Purchaser notification
33
34
35
36
3
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Product Contents
Section 1
ADAM-MC The contents of the ADAM-MC are listed below:
Accuchip kit The contents of the ADAM-MC‟s Accuchip Kit are listed below:
Upon
receiving
the
instrument
•Examine the instrument carefully for any damage incurred during
transit.
•Ensure that all parts of the instrument including accessories listed
above are included with the product.
•Any damage claims must be filed with the carrier.
•The warranty does not cover in-transit damage.
•See the 13 page to install the instrument.
•Upon receipt, store Accustain solution at room temperature.
Item Quantity
Main device 1
User manual 1
USB cable 1
Installation CD 1
Key pad 1
Power cord 1
Fuse 2
External video monitor (Optional) 1
Item Accuchip2x Kit
(Cat. No: AD2K-200)
Accuchip4x Kit
(Cat. No: AD4K-200)
Accustain
Solution
(Cat. No:
ADR-1000)
Disposable
Chip 200pcs (2 channel) 200pcs (4 channel) N/A
Solution T 2 x 12.5 ml 2 x 12.5 ml 4 x 12.5 ml
Solution N 1 x 12.5 ml 1 x 12.5 ml 2 x 12.5 ml
Available
test Q‟ty
Min. 200 test/kit Min. 400 test/kit
Max. 400 test/kit
(Only total cell count)
Max. 800 test/kit
(Only total cell count)
4
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Safety
precautions
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
Always ensure that the power supply input voltage match the voltage
available in your location.
For operation environment, See page 12.
This machine is air-cooled so its surfaces become hot during operation.
When installing it, leave a spaces of more than 10 cm (4 inches) around it.
Never insert metallic objects into the air vents of the instrument as this
could result in electrical shock, personal injury and equipment damage.
Always set the main switch on the power supply unit to off before
connecting the power cord to the wall outlet.
Always ensure that the grounding terminal of the instrument and that of
the wall outlet are properly connected. The power cord should be
connected to a grounded, 3-conductor power outlet.
To avoid potential shock hazard, make sure that the power cord is properly
grounded.
Do not position the equipment so that it is difficult to operate the
disconnecting device.
Be sure to set the main switch to off, unplug the power cord and lock the
stage before moving.
If the instrument is broken or dropped, disconnect the cord and contact a
authorized service person. Do not disassemble the instrument.
Use only authorized accessories.
Use this equipment only as specified in this manual and as specified in any
documentation associated with its components. Any use of the equipment
in an unspecified manner is strongly discouraged and may result in
damage or injury as cautioned by signed warnings.
Section 3 Safety Information
5
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Safety
symbols
The symbols used in the ADAM-MC and the manual are explained below:
The Caution symbol denotes a risk of safety hazard.
ON (Power)
Protective earth (Ground)
The CE mark symbolizes that the product conforms to all applicable European
Community provisions for which this marking is required. Operation of the
ADAM-MC is subject to the conditions described in this manual.
The protection provided by the device may be impaired if the instrument is
used in a manner not specified by the manufacturer.
Caution, Biohazard
Protective measures must be used in dealing with biologically hazardous
materials such as carcinogenic reagents.
Disposal of your old appliance
1. When this crossed-out wheeled bin symbol is attached to a product it means
the product is covered by the European Directive 2012/19/EU.
2. All electrical and electronic products should be disposed of separately from
the municipal waste stream via designated collection facilities appointed by the
government or the local authorities.
3. The correct disposal of your old appliance will help prevent potential
negative consequences for the environment and human health.
4. For more detailed information about disposal of your old appliance, please
contact your city office, waste disposal service or visit our web-site,
www.nanoentek.com.
Safety Information,continued
Section 3
6
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Warnings Battery inside device
•Risk of explosion if battery is replaced by an incorrect type.
•This battery is not replaceable by a user.
•Refer to a qualified personnel.
Cover
•Do not remove a cover or dissemble a case.
•There is no adjustable components inside the instrument.
•If malfunction is found, refer to a service personnel.
Manual
•Do not attempt to service the equipment unless
this manual has been consulted and is understood.
•This manual is available in English only.
•Failure to heed this warning may result in injury to
service provider, operator from electric shock,
mechanical or other hazards.
Sample handling
•Wear gloves during sampling. User‟s sample may
have the infectious biohazardous substance.
Waste
•After using Accuchips, appropriately dispose it as
biohazardous waste.
•Do not reuse the Accuchips.
Operator
•Must have the general knowledge of cell counting
procedure and bio safety to handle the sample that
may have the infectious biohazardous substance.
Safety Information, continued
7
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Product Specifications
ADAM-MC Voltage AC 100 ~ 240 V,
50 ~ 60 Hz
Current max. 1.8 A, max 100 W
Fuse F3.15AL250V
Objective lens 4 X
LED Green LED
CCD camera B/W CCD
Filter Excitation filter
Dichroic filter
Emission filter
Weight 9Kg
Size (W×L×H) 220 ×375 ×250 mm
Degree of
protection
IPX0
AccuChip AccuChip 2x AccuChip 4x
Measuring range 5ⅹ104~ 4ⅹ106cells/mL
Analysis time 45 sec. ~ 2 min./test
Loading sample
vol. per test
20 µ L/test (AccuChip 2X)
12 µ L/test (AccuChip 4X)
Measuring
sample vol. per
test
8.5 µ L/test (AccuChip 2X)
3 µ L/test (AccuChip 4X)
AccuStain
solution T,N
PI (Propidium Iodide) staining of
total cells (T) and non-viable cells (N).
Accessories
Power cord 1.5 m
Fuse 250 VAC, 3 A;
F3.15AL250V
Keypad USB Type / 1.3 m
Section 1
8
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Front view
of ADAM-MC
The ADAM-MC, an automated cell counter, is a benchtop automated
cell counter that performs cell count and viability measurements
using Accustain solution.
The front view showing various parts of the ADAM-MC is shown below:
•Control buttons:
Eject Ejects the chip holder from the ADAM-MC.
Start Performs all procedures of automatic counting.
Lock Protects the alignment of stage from external shock
when the ADAM-MC is moved to the other places.
☞It is strongly recommended to lock ADAM-MC before turning it off.
•Door Chip holder comes out here.
•LCD Displays the process and the result.
•keypad Inputs the sample number less than 3 characters
and press „Enter‟ button.
Door
LCD
Key Pad
Control Button
Section 1 Description of ADAM-MC
9
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Rear view
of ADAM-MC
The rear view showing various parts of the ADAM-MC.
Fan Cooling fan
Power switch Main power on/off switch
Power plug ADAM-MC power cord connection plug
USB port Connect to computer with USB Cable
RS232C port Not connect (Port for only QC and Service)
Key pad port Keypad connection port
Video Port External video monitor port
Section 1 Description of ADAM-MC,continued
Power
Switch
Power
Plug
Video
Port
USB
Port
RS232C
Port
Key Pad
Port
Fan
10
ADAM-MC (Automated Cell Counter) ⓒ2008 NanoEnTek Inc.
Overview Until now, cell counting and viability measurement for many types of cells have
been performed manually using hemocytometer with Trypan Blue exclusion
method, which is to distinguish viable cells from non-viable cells. One drawback
of this method, however, is the propensity for the staining of artifacts; another
drawback is that the naked eye can only differentiate between cells in a limited
concentration range in the hemocytometer chamber. This combined with the
potential problem of cell aggregation and limited sample volume leads to the
common variation of counts normally associated with this method.
To address these problems, NanoEnTek has developed the ADAM-MC, which is
based on a fluorescent microscopy technique for counting cells. The ADAM-MC
utilizes sensitive fluorescence dye staining, LED optics and CCD detection
technologies to make the cell analysis more accurate and reliable.
To count cells using ADAM-MC, the cells are mixed with a Propidium Iodide (PI)
stain and directly pipetted on to a disposable plastic chip. The chip is
then loaded onto a precision stage. An ADAM-MC system is automatically
focused onto the chip and cells that have been stained are recorded by
a sensitive CCD camera. The image results are automatically processed
generating the cell count which is displayed on the front of the instrument.
Simple. Fast. Accurate. Reliable.
Introduction
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