Motic AE30-31 Manual de usuario

MOTIC INCORPORATION LTD.

Prepared by Motic Instruments Inc. Canada

Episcopic-Fluorescence Attachment EF-INV-ll

Inverted Microscope AE30-31

Instruction Manual

Motic Instruments Inc. Canada

October 06, 2006

This manual may not be reproduced or transmitted in whole or in part without

Motic’s express permission.

Fluorescence

This instruction Manual has been prepared for users of the

AE-INV-ll Epi-Fluorescence Attachment used in conjunction

with the Motic AE30/31 Inverted Microscope.

We are constantly endeavouring to improve our instruments and

to adapt them to the requirements of modern research

techniques and testing methods. This involves modification to

the mechanical structure and optical design of our instruments.

Therefore, all descriptions and illustrations in this instruction

manual, including all specifications are subject to change

without notice.

Motic Instruments Inc. Canada

October 06, 2006

The optical phenomenon that occurs when light absorbed by a material, creates a molecular

excitation that causes the material to re-emit light at a different wavelength.

Fluorescence Microscope for Epi-Fluorescence

The technique of fluorescence microscopy with epi-illuminators is based on the adaptation of

the vertical illuminator used in reflected light microscopy. The radiation emitted by the light

source first passes through the exciting filter and is incident to a short-pass filter* (dichroic beam

splitter). The radiation is then reflected into the objective, which also serves as a condenser.

The objective concentrates the exciting radiation in the object field. Fluorescent light emitted

from there is collected by the objective and returned in the opposite direction to the short-pass

filter, which directs it into the eyepiece through the barrier filter.

*A filter designed to allow the passage of radiation of wavelengths shorter than a given limit.

Motic Instruments Inc. Canada

October 06, 2006

Table of Contents

1.0 Names of Component Parts

2.0 Setting up the Instrument

3.0 Assembly

4.0 Microscopy

5.0 Troubleshooting Table

6.0 Care and Maintenance

Motic Instruments Inc. Canada

October 06, 2006

1.0 Names of Component Parts

Fig. 1

Epi-fluorescence

attachment EF-INV-ll

Light source Epi-fluorescence attachment

Light protective shield

Power supply unit

Motic Instruments Inc. Canada

October 06, 2006

2.0 Setting up the Instrument

Avoid placing the instrument in areas exposed to direct sunlight, dust, vibration, high

temperature and high humidity

3.0 Assembly

3.1 Microscope assembly

Follow the instructions in the microscope manual.

For the purpose of performing simultaneous observation with phase contrast, attach the

appropriate phase contrast components referring to the specific instructions provided.

3.2 Installing the Epi-fluorescence attachment

Required tools

Allen hex keys: 1.5mm, 2.5mm and 5mm (supplied with attachment).

Please see illustrations while assembling the attachment

Turn off the microscope power switch and unplug the power cord

Before Starting

Motic Instruments Inc. Canada

October 06, 2006

3.3 Protective dust covers

See Figure 2

Pull out rear dust cover, located above the Microscope AC inlet.

Fig. 2

See Figures 3a and 3b

Remove side dust covers located on each side of the microscope base.

Fig. 3a Fig. 3b

Microscope AC inlet

Rear dust cover

Right side cover

Left side cover

Motic Instruments Inc. Canada

October 06, 2006

3.4 Attaching the Epi-fluorescence attachment sleeve

See Figure 4

Attach the main body sleeve of the epi-fluorescence attachment into the microscope

stand, with the notch on the sleeve facing left.

Fix the main body sleeve in this position by using the three 5mm Allen hexagonal

screws.

Fig. 4

Epi-fluorescence attachment

main body sleeve

Notch facing left

Motic Instruments Inc. Canada

October 06, 2006

3.5 Mounting the Epi-fluorescence attachment main body

See Figure 5

Align the pin on the Epi-fluorescence attachment with the notch in the Epi-

fluorescence attachment sleeve, slide the main body of the Epi-fluorescence

horizontally into the sleeve until it cannot be pushed in any further. To secure,

tighten the fixing screws using a hexagonal Allen key.

Fig. 5

Shutter slider with

Filter holder

Motic Instruments Inc. Canada

October 06, 2006

3.6 Installing the shutter slider with the filter holder

See Figure 6

•Loosen one of the slider knobs.

•With the filter slider slot to the left and facing the operator, insert the slider into the

opening of the main body of the fluorescence attachment.

•Tighten the loosened knob.

Fig. 6

Light cut-off slider

•To prevent the specimen from fading during temporary pauses in observation, slide

the shutter to the left.

•When using transmitted light illumination, slide the shutter to the left.

Block the optical path of the Epi-fluorescence by sliding

the shutter to the left.

Shutter slider open position

Filter slot

Tabla de contenidos

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