LI-COR LI-3100C Manual de usuario

LI-3100C

Area Meter

Instruction Manual

LI-3100C Area Meter

Instruction Manual

LI-COR Biosciences

4647 Superior Street

Lincoln, Nebraska 68504

Phone: +1-402-467-3576

Toll free: 800-447-3576 (U.S. and Canada)

[email protected]

Regional Offices

LI-COR Biosciences GmbH

Siemensstraße 25A

61352 Bad Homburg

Germany

Phone: +49 (0) 6172 17 17 771

[email protected]

LI-COR Biosciences UK Ltd.

St. John’s Innovation Centre

Cowley Road

Cambridge

CB4 0WS

United Kingdom

Phone: +44 (0) 1223 422102

[email protected]

LI-COR Distributor Network:

www.licor.com/envdistributors

NOTICE

The information contained in this document is subject to change without notice.

LI-COR makes no warranty of any kind with regard to this material, including, but not limited to the implied warranties

of merchantability and fitness for a particular purpose. LI-COR shall not be liable for errors contained herein or for incid-

ental or consequential damages in connection with the furnishing, performance, or use of this material.

This document contains proprietary information which is protected by copyright. All rights are reserved. No part of this

document may be photocopied, reproduced, or translated to another language without prior written consent of LI-COR,

inc.

Printing History:

Publication Number: 984-07704

1st Printing June, 2004

Updated September, 2021; removed information about old operating systems; minor reordering of sections

Created on: Monday, November 15, 2021

New editions of this manual will incorporate all material since the previous editions. Update packages may be used

between editions which contain replacement and additional pages to be merged into the manual by the user. The manual

printing date indicates its current edition. The printing date changes when a new edition is printed. (Minor corrections

and updates which are incorporated at reprint do not cause the date to change).

CE Marking:

This product is a CE-marked product. For conformity information, contact LI-COR Support at [email protected]. Out-

side of the U.S., contact your local sales office or distributor.

iii

Contents

Section 1. General information

Instrument function 1-1

Operational features 1-1

Section 2. Preparations

Rear housing removal 2-1

Transparent belt tension adjustment 2-1

Fluorescent tube installation 2-3

Voltage selector operation and characteristics 2-5

Section 3. Operation

Instrument activation 3-1

Display response 3-1

Belt tension and tracking adjustment 3-2

Calibration 3-2

Some history 3-2

Calibration procedure 3-3

Pressing roller adjustment 3-4

Section 4. Changing measurement resolution

Changing from 1.0 mm² to 0.1 mm² resolution 4-1

Changing from 0.1 mm² to 1.0 mm² resolution 4-3

Section 5. Using the Windows® interface software

Installing the software 5-1

Connecting the LI-3100C to a computer 5-1

Software basics 5-3

Creating a log file 5-4

Missed data packets 5-6

Importing a data file to a spreadsheet 5-7

Using the LI-3000A readout 5-7

Section 6. Troubleshooting

Continuous spurious data accumulation 6-1

Belts not tracking properly 6-2

Fluorescent tube fails to illuminate 6-2

Excessive data variability 6-2

Miscellaneous malfunctions 6-3

Laboratory conditions 6-3

Section 7. Maintenance

Changing the fluorescent tube 7-1

Changing the transparent belts 7-1

Alternative belt removal technique 7-3

Replacing the transparent belts 7-3

Cleaning the transparent belts 7-4

Cleaning the mirror or camera lens 7-4

Cleaning the interior of the camera 7-4

Cleaning the motor and belt drive system 7-4

Section 8. Removing the upper belt assembly

Appendix A. Specifications

Appendix B. Warranty

Section 1.

General information

The LI-3100C Area Meter precisly measures area (length and width) of objects in bio-

logical, industrial, and other applications.

Instrument function

Samples are placed between the guides on the lower transparent belt and allowed to

pass through the instrument. As items travel under the 15 watt fluorescent light

source, the object is reflected by a system of three mirrors to a solid state scanning

camera within the rear housing.

Width is sensed by the scanning camera. Length information is provided by the cur-

rent frequency as related to the belt velocity. Area integration is accomplished by

components of the main printed circuit board mounted on the instrument rear plate

(Figure 2-1 on page2-2).

Data are presented on the light emitting diode (LED) display. Decimal location on

the display is changed to suit the 1.0 or 0.1 mm2resolution requirement (Changing

measurement resolution on page4-1). Alternatively, data can be collected, displayed,

and stored using a PC (see Using the Windows® interface software on page5-1).

Operational features

The LI-3100C features interchangeable 1.0 mm2and 0.1 mm2resolution capability

that is furnished with a single 28 mm camera lens. A 25 cm wide sample guide is

provided for both 1.0 mm2and 0.1 mm2resolution configurations.

The upper belt assembly can be removed for operations hindered by the upper belt.

Sample thickness can then be increased to 2.5 cm (see Removing the upper belt

assembly on page8-1).

1-1LI-3100C Area Meter Manual

Section 1. General information

1-2 LI-3100C Area Meter Manual

Section 2.

Preparations

The following procedures must be performed before using the LI-3000C for the first

time:

lTighten the transparent belts

lInstall the 15 watt fluorescent tube

lRemove the lens cover

lSet the voltage selector at the proper position and check to see if the correct fuse

is installed

Each of these procedures is discussed below.

Rear housing removal

The rear housing must be removed in order to adjust the belt tension and to install

the fluorescent tube. Using the 5/32" (4 mm) hexagonal key provided, remove the

four retaining screws (one at each end, one at the center front edge on top, and one

at the center bottom edge at the rear). Lift the housing from the instrument.

Transparent belt tension adjustment

Perform the following steps to tighten the transparent belts.

1Loosen the large knurled knobs located on each sliding pulley bearing block.

Rear sliding bearing blocks are visible in Figure 2-1 on the next page. Front sliding

bearing blocks are visible in Figure 2-2 on page2-3.

2Coarse adjustment is performed by simultaneously sliding both bearing blocks for one pulley.

2-1LI-3100C Area Meter Manual

Section 2. Preparations

The knurled knobs can be tightened sufficiently by rotating them with the thumb

and index finger while holding tension on the bearing block with the remaining fin-

gers of the same hand.

3Fine adjustments are performed by twisting the block adjustment screw to push the sliding bear-

ing block.

The knurled knobs located on the pulley bearing blocks must be loosened to allow

block movement.

Figure 2-1. Area Meter with rear housing removed. (A) Main printed circuit board, (B) rear

sliding bearing blocks, (C) decimal selector switch set for 1.0 mm2or 0.1 mm2operation

(inset), (D) 28 mm lens, (E) screws on the outer camera pressure rail, (F) slide this window

to the left to inspect the voltage selector, (G) upper drive pulley gear.

2-2 LI-3100C Area Meter Manual

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