Leica DMIL Manual de usuario
Leica DMIL
Instructions · Bedienungsanleitung
Mode d’emploi
Phone/Tel./Tél. +49 (0) 6441-292519
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Issued in 1998 by/
Herausgegeben 1998 von/
Edition 1998 par:
Leica Microsystems Wetzlar GmbH
Ernst-Leitz-Strasse
D-35578 Wetzlar (Germany)
Responsible for contents/
Verantwortlich für den Inhalt/
Département responsable du contenu:
Marketing MQM, Product Management
3
Leica
DM IL
Instructions
4
Copyrights
All rights to this documentation are owned by
Leica Microsystems Wetzlar GmbH. Copying of
text and illustrations – in full or in part – by print-
ing, photostat, microfilm or other techniques,
including electronic systems, is only permitted
subject to the express written consent of Leica
Microsystems Wetzlar GmbH.
The information contained in the following
documentation represents the latest stage of
technology and knowledge. We have composed
the texts and illustrations with great care.
However, as it is impossible to eliminate the risk
of error completely, we cannot accept any kind
of liability for the correctness of the contents of
this manual. Nevertheless, we are always grate-
ful to be notified of any errors.
The information in this manual may be altered
without prior notice.
5
Contents
Technical description........................................ 67
Objectives ............................................................. 67
Eyepieces ............................................................. 70
Filters ..................................................................... 72
Tubes ..................................................................... 73
Condensers .......................................................... 75
Lamps and lamp housings ................................. 76
Specifications ...................................................... 78
Main wear and replacement parts ................. 80
EU Declaration of Conformity .......................... 81
Important notes ................................................... 6
General safety information............................... 7
Application .......................................................... 9
The microscope and its components ............. 10
Installation site ................................................... 15
Unpacking ............................................................ 15
Setting up ............................................................. 17
Setting up the options ........................................ 26
Operation .............................................................. 39
Basic settings for transmitted light ................. 39
Operation of objectives...................................... 42
Operation of transmitted light........................... 43
Operation of phase contrast ............................. 45
Operation of Integrated
Modulation Contrast (IMC) ............................... 47
Operation of incident light fluorescence ....... 51
Care and maintenance ...................................... 56
Troubleshooting
(lamp/fuse replacement) ................................... 58
Storage.................................................................. 66
Packaging and transport................................... 66
6
The manual is multi-lingual. The spiral binding
makes it possible to turn the instructions in the
desired language to the front.
Numbers in brackets, e. g. (1.2), refer to
illustrations, in the example to Fig. 1, pos. 2.
Numbers with an arrow, e. g. → p. 20, refer to a
particular page of this manual.
Special safety information is marked with a
triangular symbol in the margin and printed
on a grey background.
Attention! Operating errors can damage the
microscope or its accessories.
Warning of hot surface.
Explanatory note.
Not part of all configurations.
Important notes
(1.2)
→ p. 20
!
*
This manual is an important part of the Leica
DM IL microscope and should be carefully
read before switching on and using the
instrument.
This manual contains important instructions and
information with regard to operating safety and
maintenance of the system. For this reason, it
should be carefully kept at hand.
Text symbols and their meaning:
7
General safety information
Only fuses of the specified type and rating may
be used as replacements. Never use repaired
fuses or short-circuited fuse holders.
Attention!
The instruments and accessories described
in this manual have been safety-tested and
checked for possible hazards.
Before modifying the instrument in any way
or combining it with non-Leica products not
covered by this manual, please always
contact your local Leica representative or the
main factory in Wetzlar!
Any unauthorised interference with the
instrument or use of the instrument for
applications for which it is not designed will
automatically void any warranty claim!
This instrument of Safety Category I has been
built and tested according to EN 61 010-1/
IEC 1010-1, Safety Standards for Electronic
Measuring Instruments, Electronic Regulators
and Electronic Laboratory Instruments.
Attention!
To maintain this condition and to ensure safe
operation, the user must note and adhere to
the directions and warnings contained in this
manual.
The mains plug may only be inserted into a
grounded socket.
The protection must not be jeopardised by using
an extension cable without ground conductor.
Any break in the ground conductor within or
outside the instrument or loosening of the
ground connection can render the unit
dangerous. Intentional severance is prohibited!
Attention!
Accessory devices connected to the
microscope which have a separate and/or
different power supply should be brought to
the same ground potential by connecting
them to the same grounding system. Contact
the service department in case of an
ungrounded mains supply .
8
Attention!
The electrical accessories for the micro-
scope are not protected against the
penetration of water which can result in
electrical shock.
Do not set up the microscope and its
accessories in the immediate vicinity of a
water outlet or in other places where water
may penetrate into the instrument.
Attention!
Before replacing fuses or lamps, always
switch off the power switch and disconnect
the power cord.
Attention!
Protect the microscope against excessive
temperature fluctuations which may lead to
condensation and thus damage the electrical
and optical components.
Attention!
When using immersion oil, take care to avoid
skin contact! Ask the supplier for a safety
data sheet!
9
Application
The Leica DM IL microscope is designed for
routine examinations of cell and tissue cultures,
liquids, and sediments.
Two basic microscope stands are available for
biologic applications. One is the standard bright
field version using the contrasting methods
bright field (BF), phase contrast (Phaco), and
Integrated Modulation Contrast (IMC), the other
is the fluorescence microscope which also
offers incident-light fluorescence in addition
to the three transmitted-light contrasting
techniques.
All microscope techniques and the required
accessories for the Leica DM IL are described
and explained in detail in the Operation section
of this manual, including the associated
functions and their use.
10
15
13
12
21 22
24
14
20
17
11
23
The microscope and its components
Fig. 1 Right side of microscope stand
Fig. 2 Left side of microscope stand
Fig. 3
Microscope stand with DM L tubes and discussion facility
26
25
28 26
27
Fig. 1 – 3
1 Binocular photo tube DM ILT, 2 Eyepiece tube, 3 Eyepieces,
4 Tube holder, 5 Empty slide or IMC modulator, 6 Integrated
lamp housing with halogen lamp 6 V/35 W, 7 Holder for filter
Ø32 mm, 8 Empty slide or modulation or phase contrast slide,
9 Aperture diaphragm, 10 Condenser S 55, 11 Transmitted-
light illumination carrier, 12 Notched lever for condenser
level adjustment, 13 Transmitted-light illumination column,
14 Specimen stage, 15 Objective nosepiece and objectives,
16 Brightness control, 17 Coarse control adjustment and
micrometer adjustment, 18 Power switch, 19 Fluorescence
filter blocks, 20 Fluorescence lamp housing, 21 Light stop,
22 BG9 Filter, 23 Stabiliser plate, 24 c-mount video adapter,
25 Tube adapter IL/L, 26 DM L tubes, 27 Multi-discussion
facility, 28 Condenser S 90
Main assemblies
The following overall views show and designate
the main assemblies of the microscope and its
accessories.
1
2
3
4
19 18
76
9
8
10
5
16
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