Leica DMIL Manual de usuario

Leica DMIL

Instructions · Bedienungsanleitung

Mode d’emploi

Phone/Tel./Tél. +49 (0) 6441-292519

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Issued in 1998 by/

Herausgegeben 1998 von/

Edition 1998 par:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Strasse

D-35578 Wetzlar (Germany)

Responsible for contents/

Verantwortlich für den Inhalt/

Département responsable du contenu:

Marketing MQM, Product Management

Leica

DM IL

Instructions

Copyrights

All rights to this documentation are owned by

Leica Microsystems Wetzlar GmbH. Copying of

text and illustrations – in full or in part – by print-

ing, photostat, microfilm or other techniques,

including electronic systems, is only permitted

subject to the express written consent of Leica

Microsystems Wetzlar GmbH.

The information contained in the following

documentation represents the latest stage of

technology and knowledge. We have composed

the texts and illustrations with great care.

However, as it is impossible to eliminate the risk

of error completely, we cannot accept any kind

of liability for the correctness of the contents of

this manual. Nevertheless, we are always grate-

ful to be notified of any errors.

The information in this manual may be altered

without prior notice.

Contents

Technical description........................................ 67

Objectives ............................................................. 67

Eyepieces ............................................................. 70

Filters ..................................................................... 72

Tubes ..................................................................... 73

Condensers .......................................................... 75

Lamps and lamp housings ................................. 76

Specifications ...................................................... 78

Main wear and replacement parts ................. 80

EU Declaration of Conformity .......................... 81

Important notes ................................................... 6

General safety information............................... 7

Application .......................................................... 9

The microscope and its components ............. 10

Installation site ................................................... 15

Unpacking ............................................................ 15

Setting up ............................................................. 17

Setting up the options ........................................ 26

Operation .............................................................. 39

Basic settings for transmitted light ................. 39

Operation of objectives...................................... 42

Operation of transmitted light........................... 43

Operation of phase contrast ............................. 45

Operation of Integrated

Modulation Contrast (IMC) ............................... 47

Operation of incident light fluorescence ....... 51

Care and maintenance ...................................... 56

Troubleshooting

(lamp/fuse replacement) ................................... 58

Storage.................................................................. 66

Packaging and transport................................... 66

The manual is multi-lingual. The spiral binding

makes it possible to turn the instructions in the

desired language to the front.

Numbers in brackets, e. g. (1.2), refer to

illustrations, in the example to Fig. 1, pos. 2.

Numbers with an arrow, e. g. → p. 20, refer to a

particular page of this manual.

Special safety information is marked with a

triangular symbol in the margin and printed

on a grey background.

Attention! Operating errors can damage the

microscope or its accessories.

Warning of hot surface.

Explanatory note.

Not part of all configurations.

Important notes

(1.2)

→ p. 20

This manual is an important part of the Leica

DM IL microscope and should be carefully

read before switching on and using the

instrument.

This manual contains important instructions and

information with regard to operating safety and

maintenance of the system. For this reason, it

should be carefully kept at hand.

Text symbols and their meaning:

General safety information

Only fuses of the specified type and rating may

be used as replacements. Never use repaired

fuses or short-circuited fuse holders.

Attention!

The instruments and accessories described

in this manual have been safety-tested and

checked for possible hazards.

Before modifying the instrument in any way

or combining it with non-Leica products not

covered by this manual, please always

contact your local Leica representative or the

main factory in Wetzlar!

Any unauthorised interference with the

instrument or use of the instrument for

applications for which it is not designed will

automatically void any warranty claim!

This instrument of Safety Category I has been

built and tested according to EN 61 010-1/

IEC 1010-1, Safety Standards for Electronic

Measuring Instruments, Electronic Regulators

and Electronic Laboratory Instruments.

Attention!

To maintain this condition and to ensure safe

operation, the user must note and adhere to

the directions and warnings contained in this

manual.

The mains plug may only be inserted into a

grounded socket.

The protection must not be jeopardised by using

an extension cable without ground conductor.

Any break in the ground conductor within or

outside the instrument or loosening of the

ground connection can render the unit

dangerous. Intentional severance is prohibited!

Attention!

Accessory devices connected to the

microscope which have a separate and/or

different power supply should be brought to

the same ground potential by connecting

them to the same grounding system. Contact

the service department in case of an

ungrounded mains supply .

Attention!

The electrical accessories for the micro-

scope are not protected against the

penetration of water which can result in

electrical shock.

Do not set up the microscope and its

accessories in the immediate vicinity of a

water outlet or in other places where water

may penetrate into the instrument.

Attention!

Before replacing fuses or lamps, always

switch off the power switch and disconnect

the power cord.

Attention!

Protect the microscope against excessive

temperature fluctuations which may lead to

condensation and thus damage the electrical

and optical components.

Attention!

When using immersion oil, take care to avoid

skin contact! Ask the supplier for a safety

data sheet!

Application

The Leica DM IL microscope is designed for

routine examinations of cell and tissue cultures,

liquids, and sediments.

Two basic microscope stands are available for

biologic applications. One is the standard bright

field version using the contrasting methods

bright field (BF), phase contrast (Phaco), and

Integrated Modulation Contrast (IMC), the other

is the fluorescence microscope which also

offers incident-light fluorescence in addition

to the three transmitted-light contrasting

techniques.

All microscope techniques and the required

accessories for the Leica DM IL are described

and explained in detail in the Operation section

of this manual, including the associated

functions and their use.

21 22

The microscope and its components

Fig. 1 Right side of microscope stand

Fig. 2 Left side of microscope stand

Fig. 3

Microscope stand with DM L tubes and discussion facility

28 26

Fig. 1 – 3

1 Binocular photo tube DM ILT, 2 Eyepiece tube, 3 Eyepieces,

4 Tube holder, 5 Empty slide or IMC modulator, 6 Integrated

lamp housing with halogen lamp 6 V/35 W, 7 Holder for filter

Ø32 mm, 8 Empty slide or modulation or phase contrast slide,

9 Aperture diaphragm, 10 Condenser S 55, 11 Transmitted-

light illumination carrier, 12 Notched lever for condenser

level adjustment, 13 Transmitted-light illumination column,

14 Specimen stage, 15 Objective nosepiece and objectives,

16 Brightness control, 17 Coarse control adjustment and

micrometer adjustment, 18 Power switch, 19 Fluorescence

filter blocks, 20 Fluorescence lamp housing, 21 Light stop,

22 BG9 Filter, 23 Stabiliser plate, 24 c-mount video adapter,

25 Tube adapter IL/L, 26 DM L tubes, 27 Multi-discussion

facility, 28 Condenser S 90

Main assemblies

The following overall views show and designate

the main assemblies of the microscope and its

accessories.

19 18

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