Integra MINI 96 Manual de usuario

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Quick Start Guide MINI 96 −96 Channel Pipette

This quick start guide is intended to provide a quick overview of your MINI 96’s

key features and to offer basic instructions for getting started. For detailed

information, please refer to the latest operating instructions that can be found at

www.integra-biosciences.com in different languages.

Use this QR code or visit www.integra-biosciences.com/en/mini-96-getting-

started to access the getting started video.

Intended use

This is a general-purpose laboratory instrument. Any use of this instrument in a medical or IVD

setting is the sole responsibility of the user. MINI 96 are electronic handheld pipettes that can be

used for aspirating and dispensing aqueous solutions in the volume range of 0.5 μl to 1250 μl using

GRIPTIPS® pipette tips only, see www.integra-biosciences.com.

Safety information

Regardless of the listed safety notes, all locally applicable regulations must be observed.

1) The pipette may only be used by properly trained personnel in a manner specified by INTEGRA

Biosciences.

2) Do not use the instrument near flammable material or in an atmosphere with danger of

explosion.

3) Do not immerse the pipetting unit in liquid. Avoid pipetting of liquids emitting corrosive vapors.

4) Servicing work andrepairs may only be performed by INTEGRA Biosciences or an authorized

after-sales service member.

Getting started

Hold theMINI 96 by thecarrying grip and handle (a, g) and set it up on a perfectly

horizontal surface.

For shipping the instrument, clear the deck, select park head and press OK. The

pipetting unit automatically moves to park position. Use the original packaging.

Insert the adapter cable in the socket (l) and connect the mains adapter to the electricity mains.

Supply voltage: 100  60 Hz– 240 VAC, 50 –  .

a. Carrying grip

b. Base stage on deck

c. Magnetic covers, for second stage

d. LED bars

e. Deck light

f. Control unit

g. Carrying handle

h. Pipetting unit, to move up and down

i. AUX port

j. USB-C port

k. Main switch (ON ǀ OFF)

l. DC Input for mains adapter

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MINI 96 control unit

m. Display

n. Touch wheel, spin to scroll the cursor

o. OK button, to make a selection

p. RUN button, to start operations

q. Tip ejector

r. PURGE button, to empty tips

s. Arrow buttons, for selections

t. Back button, to navigate backward

Loading GRIPTIPS

•Insert the base stage (b) on the deck. “96” or “384” must match the tip

rack pattern. Put a tip rack on the base stage.

•To load 96 tips four times from racks of 384tips (12.5 μl and 125 μl only),

move the tip rack in one of the four corners, e. g. front left.

•Hold the control unit (f) and lower the pipetting unit (h) down onto the

tip rack until you are prompted to press OK to start automated tip loading.

•Partial tip loading requires the optional two position stage. Press Partial ► and Set No. of

Columns ►. Enter the number of columns filled with tips and press OK.

Setting pipetting height

•To define the lowest possible pipetting height, for ex.

the bottom of a well plate, select Pipetting Height .

•With tips attached move the pipetting unit down to the

desired pipetting height.

•Press Set to save yoursetting.

Running a pipetting program

Select from predefined programs that youcan adapt easily orcreatemulti-stepped customprograms.

Program

Description

Pipet

Liquid transfers when aspirate and dispense volumes are equal.

Repeat Dispense

Dispense multiple aliquots of the same volume.

Sample Dilute

Aspirate two liquids divided by an air gap, followed by dispense.

Pipet/Mix

Multiple mixing by aspiration and dispensing of defined volume.

Manual Pipet

Control the aspiration and dispensing up to the set volume.

Reverse Pipet

Liquid transfers of viscous or high vapor pressure liquids.

Variable Dispense

Dispense multiple aliquots of different volumes.

Multi Aspirate

Aspirate multiple aliquots of different volumes.

Sample Dilute/Mix

Aspirate two liquids divided by an air gap, followed by dispense and mix.

Serial Dilution

Aspirates a transfer volume followed by a mix.

Custom

Creates and store multi-stepped pipetting protocols.

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Active run step (orange)

Parameters are listed

in the lower half

From the Main Menu, use the

touch wheel to scroll to your

desired program.

Press OK to enter the selected

program.

Insert the GRIPTIPS into the liquid to be transferred.

Press and release RUN to aspirate the volume

selected in the first step.

Your pipette will prompt you to press RUN at each

successive step.

Setting/changing parameters

Press OK to Edit the program.

A list of editablesteps

is displayed.

Select a step and press OK.

Use the touchwheel to set the

value and press OK.

Press to save your settings.

Installation of second stage

The second stage can be used as a second position, e.g., place source

liquids on the base stage and slid a well plate on the second stage for

filling.

•Remove two magnetic covers (c) by using a GRIPTIP.

•Insert the second stage with the desired side (96 or 384 format) facing

upwards.

Two Step Blowout

During aspiration, the piston of your electronic pipette moves up (1).

During dispensing, the piston returns to the initial position (0). During the last

dispense of a program, it automatically moves further down (2) and blows the

remaining liquid from the tip (Blowout).

When the piston returns to the initial position (0), a small amount of air is

aspirated, provided the tip is no longer immersed in the liquid (Blowin).

Note: Manually delay the blowin by holding RUN pressed during the last dispense. Remove the

tips from the vessel and release RUN to start blowin.

Maintenance

Always switch off the device and disconnect from the electricity supply when carrying out

maintenance work.

Clean the MINI 96 housing with a lint-free cloth lightly soaked with mild soap solution in distilled

water or with a 70 % solution of isopropanol or ethanol.

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The device may be decontaminated with H2O2gas (maximal concentration35 %) for 60 minutes.

If you intend to ship the MINI 96 to be periodically calibrated, the original packaging can be stored

and reused for this purpose. Contact INTEGRA for more information about calibration services.

Equipment disposal

MINI 96 must not be disposed of with unsorted municipal waste. Dispose of MINI 96 in

accordance with the regulations in your area governing disposal of devices.

In certain regions and countries, e.g., in EU member states, the distributor is obliged to

take back this product free of charge at the end of life. Please contact your local distributor

for more details.

Manufacturer and customer service

INTEGRA Biosciences AG

Tardisstrasse 201

CH-7205 Zizers, Switzerland

INTEGRA Biosciences Corp.

22 Friars Drive

Hudson, NH 03051, USA

Find your local representative:

www.integra-biosciences.com

info@integra-biosciences.com

Direct sales country

Telephone

Mail

China

+86 21 5844 7203

info-cn@integra-biosciences.com

Denmark, Sweden

+45 3173 5373

info-nordic@integra-biosciences.com

France

+33 1 34 30 76 76

info-fr@integra-biosciences.com

Germany, Austria

+49 6409 81999 15

info-de@integra-biosciences.com

Japan

+81 3 5962 4936

info-jp@integra-biosciences.com

Switzerland

+41 81 286 95 30

info-ch@integra-biosciences.com

United Kingdom

+44 1635 797000

info-uk@integra-biosciences.com

United States, Canada

+1 603 578 5800

info-us@integra-biosciences.com

Declaration of conformity

INTEGRA Biosciences AG –7205 Zizers, Switzerland

declares on its own responsibility that the devices

Description

Models

MINI 96

4801, 4802, 4803, 4804

Mains adapter

128909

comply with:

EU directives and regulations

2014/35/EU, 2014/30/EU, 2012/19/EC, 2011/65/EC, 1907/2006, 2019/1782

GBR regulations

S.I. 2016/1101, S.I. 2016/1091, S.I. 2013/3113, S.I. 2012/3032

Zizers, April 11, 2022

Urs Hartmann

CEO

Daniel Bächi

Head of Corporate Quality

For detailed CE declaration and regulations of othercountries, please refer to the operating

instructions.

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