InnovaPrep AIRPREP Cub Sampler ACD 210 Manual de usuario

AirPrep Cub Sampler™ (ACD 210 & ACD 220)

and Air Sampling System

User Manual

Rev B

Table of Contents

1Product Overview...................................... 3

1.1 Product Description ................................................................................................................................................ 3

1.2 Collection Capability ............................................................................................................................................... 3

1.3 Materials Provided ................................................................................................................................................. 3

1.4 Materials Required But Not Included..................................................................................................................... 3

2Method of Operation ................................ 4

2.1 Sample Collection on an Electret Filter .................................................................................................................. 4

2.2 Instant Wet Foam Elution™.................................................................................................................................... 4

3Sampler Components ................................ 5

3.1 AirPrep Cub Sampler Components ......................................................................................................................... 5

4Procedures................................................. 6

4.1 Setting Up the AirPrep Cub Sampler for Collection ............................................................................................... 6

4.2 Installing the Filter .................................................................................................................................................. 6

4.3 Control Panel Introduction ..................................................................................................................................... 6

4.3.1 LED Indicator Light .............................................................................................................................6

4.3.2 Power on ........................................................................................................................................... 7

4.3.3 Powering off Manually before time Expires ......................................................................................7

4.3.4 Power Off Due to Low Battery* ........................................................................................................ 7

4.3.5 Selecting the flow rate ...................................................................................................................... 8

4.3.6 How to select a timed run mode........................................................................................................8

4.4 Recovery of the Sample.......................................................................................................................................... 8

4.5 Eluting the Sample from the Filter ......................................................................................................................... 8

4.6 Cleaning the Sampler.............................................................................................................................................. 8

5AirPrep Cub Sampler- Model ACD210 Battery safety information 9

6Specifications........................................... 10

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AirPrep™ Cub Sampler ACD210 model has an internal battery

AirPrep™ Cub Sampler ACD220 model is plug-in only

1PRODUCT OVERVIEW

1.1 PRODUCT DESCRIPTION

The AirPrep Cub Sampler is a dry filter air sampler that operates using InnovaPrep’s unique, patented rapid filter elution products to

provide samples for a wide range of indoor and outdoor biomonitoring applications.

1.2 COLLECTION CAPABILITY

The AirPrep Cub Sampler is suited for the collection of bioaerosols (bacteria, molds, and viruses) and other particulate matter, including

sub-micron sized particles, and airborne molecular contamination. The AirPrep Cub Sampler efficiently captures micron- and

submicron-sized particles, including viruses, bacteria, pollen, molds and fungal spores, as well as non-biological particles from 0.01

micron to 10+ microns.

•Users can select from three flowrate modes: 50 LPM, 100 LPM, or 200 LPM from the front panel as described in Section 4.

•Users can select from four timed modes: 30 minutes, 1 hour, 2 hours, or continuous from the front panel as described in

Section 4.

•Since liquids are not used in the collector, the system can be operated in extreme temperatures (see specifications in Section

6).

1.3 MATERIALS PROVIDED:

The AirPrep Cub Sampler includes the power supply cord (Item #AC04076) and a universal fitting installed in the base of the unit for

common off-the-shelf camera tripods.

*Upon receipt, always inspect components when unpacking. If damage is observed, do not use and contact InnovaPrep or local

distributor immediately. Please use the original box and foam packaging in the event a return shipment is necessary.

1.4 MATERIALS REQUIRED BUT NOT INCLUDED-SEE AirPrep Consumables Selection Guide for more information.

The single-use AirPrep Filter & Elution Kit comes with everything you need to recover a fluid sample (as shown in Figure 1).

Users have a choice between two kits with different elution buffer types:

•AirPrep Filter & Elution Kit with TRIS - Item AC8100T - Recommended for use with rapid

molecular methods of analysis.

•AirPrep Filter & Elution Kit with PBS - Item AC8100P - Recommended for classical culture

analysis.

The AirPrep Filter & Elution Kit includes:

1. Elution Buffer Canister

2. Elutor Adapter

3. Filter

4. Sample Cup

These kits and multipacks are available for purchase on the InnovaPrep website

https://www.innovaprep.com/store .FIGURE 1 AIRPREP

FILTER & ELUTION KIT

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2METHOD OF OPERATION

The AirPrep Cub Sampler uses an electret filter to capture particles. To recover the trapped particles, a novel Wet Foam Elution™

process is employed to break the electrostatic charge and flush the particles off the filter into a small liquid volume in seconds.

2.1 SAMPLE COLLECTION ON AN ELECTRET FILTER

The dry 52 mm electret filters are made with a combination of positively charged and negatively charged fibers. This substantially

increases the collection efficiency of the filter and allows for the use of lower pressure drop filters, which, in turn, allows for higher

sampling rates for extended periods using battery power.

2.2 INSTANT WET FOAM ELUTION™

The patented wet foam elution process uses a very specific high-quality foam to elute the filter. The elution buffer is composed of

water, a low concentration surfactant (0.15% Tween 20), and a pH buffer (Tris or PBS). This solution is carbonated, which dissolves

significant amount of CO2 into the fluid. During the elution process, the valve opens as the elution canister is pushed into the elutor

adapter that is fitted onto the filter, forcing the elution buffer through the pressure orifice, through the filter and into the sample

cup. As the fluid passes from the high-pressure environment on one side of the orifice, to the low-pressure environment on the

other side, the dissolved CO2 expands, comes out of the solution to form micro-bubbles. These micro-bubbles increase the volume

of the fluid six-fold or more as it passes through the filter. The foam immediately collapses back into a liquid and is ready for sample

processing and analysis. InnovaPrep’s patented Wet Foam Elution process (https://www.innovaprep.com/patents) is much more

efficient than liquid rinsing for the following reasons:

•Volume Expansion - When rinsing a filter with liquid, most of the liquid volume is used to fill the dead space inside the filter

housing; only a small portion of the fluid covers the filter surface. This can be avoided to an extent by reducing the cross-sectional

area of the fluid path across the filter, but a large portion of the liquid is still underutilized. Because our elution buffer is 80-90%

gas, this fills the empty space without contributing to the final sample volume.

•Increased Viscosity - Liquid has a tendency toward “channeling” when flowing through a filter, creating an area of high flow in

the center of the fluid path, while the portion of flow in contact with the filter surface is much slower. The higher viscosity of our

elution buffer as a foam prevents channeling and allows for a more uniform flow through the interstitial spaces of the filter.

•Bubble Dynamics - The micro-bubbles in the foam behave as deformable solids. As they travel through the filter they move as a

rigid body.

•Exfoliating Action - As the micro-bubbles in the foam impact against each other and burst, the turbulence and energy produced

helps to lift particles that are adhering to the filter fibers.

•Buffered Elution - The buffered elution fluid is conductive and breaks down the electrostatic charges holding the particles to the

electret filter, effectively releasing them in the liquid sample.

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3SAMPLER COMPONENTS

3.1 AIRPREP CUB SAMPLER COMPONENTS

1. Omni-Directional Inlet Cap – protects from water ingress

2. Inlet - screws off left to open for loading or recovering the filter 1.

cartridge. Screws on right to close

3. Front Panel - Power on and Mode selections (see section 4 for

instructions) 2.

4. Power Supply and Plug

5. Universal Tripod Connection - for attaching to a common,

off-the-shelf camera tripod is located on the bottom of the

instrument (not shown)

FIGURE 2 SAMPLER

COMPONENTS

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4PROCEDURES

This section includes some of the basic instructions for operation.

4.1 SETTING UP THE AIRPREP CUB SAMPLER FOR COLLECTION

Push the barrel plug of the power supply (Figure 2) into the power jack on the bottom of the instrument. Plug the AC Power Cord into

the power supply and plug the other end into an appropriate wall outlet.

4.2 INSTALLING THE FILTER

Open the inlet cap by twisting it to the left to lift off. Insert the filter into the top of the AirPrep Cub Sampler, as shown in figure 2. The

filter housing is designed to only fit in one way. Screw the inlet lid on (twisting right) to close.

4.3 CONTROL PANEL INTRODUCTION

The control panel consists of four timer setting LED indicator lights, three flowrate LED indicator lights,

and one momentary pushbutton switch (Hold/Mode).

Two variables constitute a “run mode”: flowrate and runtime.

To turn on and off:

• Press Hold/Mode for 2 seconds to Power on/power off

To change run modes

• press the Hold/Mode for 4 seconds

Reset Button

• A reset button is located on the bottom of each instrument, pressing this will result in an

immediate system interrupt and reset of the system.

4.3.1 LED INDICATOR LIGHT

FIGURE 3 CONTROL

PANEL

The Battery LED Indicator Light (Model 210 only) will flash in the following situations:

•

When a run mode is selected that exceeds the expected battery capacity

•

After the battery is drained low enough to shut down the fan before the timer expired

•

The battery LED indicator light will start to flash slowly as the charge approaches a critical level- increasing in

frequency until it is steady on when the battery is at its preset critical charge.

The

battery LED indicator will turn solid if the power supply is plugged in and the system is charging. It will shut off

when it is fully charged.

Battery

Status Indicator

By holding the power button for 2 seconds, then release, the Power LED will light up solid, followed by a combination

the five green LEDs on the left side of the panel. Each green LED that is lit indicates 20% of the battery. Example: If

of the 5 green LEDs are lit this would indicate approximately a 60% battery charge.

Empty

60% Charge

100% Charge

Rev B

4.3.4 P

OWER

UE TO

ATTERY

*Model 210 only

FIGURE 5. POWER-OFF DUE TO LOW BATTERY (MODEL 210)

Flowrate LED Indicator Light

The flowrate LED indicator for the selected run mode will stay on while the fan is running.

Timer LED Indicator Light

• The timer LED Indicator light will flash slowly as they countdown, as they approach the expiration they will increase in frequency

until the unit times out and shuts down. The light will remain solid for the last 10% of the timer.

• If the timer is set to continuous, the LED indicator light will stay steadily on.

4.3.2 POWER ON

When powering-on the sampler, the previous run mode will be recalled and started by default.

FIGURE 4. POWER-ON

4.3.3 POWERING OFF MANUALLY BEFORE TIME EXPIRES

To power-off the Air Sampler before the time expires, hold the Hold/Mode button for 2 seconds, the unit will power-off.

Rev B

If a time & flowrate combination requires more battery than is available, the battery LED will flash to alert you

4.3.5 SELECTING THE FLOW RATE

1. Press Hold/Mode button for 4 seconds, if fan was already running it will shut off after the first two seconds.

a. All 3 Flowrate LED Indicator lights will flash

2. Let up and momentarily press the Hold/Mode button

a. The 50LPM LED indicator light will flash. If desired, stop to select or

3. Momentarily press the Hold/Mode button again

a. The 100LPM LED indicator light will flash. If desired, stop to select.

Additional momentary presses will continue to cycle through the 50, 100 and 200 LPM LEDs

4. Press the Hold/Mode button for 2 seconds to save. Selected flowrate mode is saved, and LED indicator light illuminates steadily;

all timer LED indicator lights start flashing

4.3.6 HOW TO SELECT A TIMED RUN MODE

1. Once the flow rate has been set. Press the Hold/Mode button momentarily.

a. All four timed mode LED indicator lights will flash

2. Let up and momentarily press the Hold/Mode button

a. The 30 Min LED indicator light will flash. If desired, stop to select or

3. momentarily press the Hold/Mode button again

a. The 1-hour LED indicator light will flash. If desired, stop to select.

Additional momentary presses will continue to cycle through the remaining timer modes.

4. Press Hold/Mode button for 2 seconds to save selection. Selected flowrate mode is saved and LED indicator light illuminates.

a. Fan will start.

4.4 RECOVERY OF THE SAMPLE

1. When the run is completed, recover the filter from the sampler with gloved hands by twisting the inlet to the left to open.

2. Remove the filter cassette, invert it top-side-down, and press onto the sample cup firmly until it clicks all the way around the

cup. Perform this on a stable surface.

3. Cap the filter with the elutor adapter as shown in Figure 6 (make sure the filter and elutor adapter are fully sealed).

4. Place the filter assembly in a zip-type bag for transporting dry, or alternatively, elute the filter as described in the following

section.

4.5 ELUTING THE SAMPLE FROM THE FILTER

To extract the captured particles from the filter:

1. Place the sample cup, filter, and elutor adapter, as shown in Figure 6, on a stable surface.

2. Firmly press the elution buffer canister into the elutor adapter. The elution buffer will be

released as a foam from the canister through the filter when the canister valve is pressed

down. Hold the canister down until all the foam is released. The foam quickly collapses into a

liquid in the sample cup and available for sample processing and analysis.

4.6 CLEANING THE SAMPLER

FIGURE 6 AIRPREP

FILTER & ELUTION KIT

Common off-the-shelf alcohol or bleach wipes can be used for cleaning and disinfection of the entire sampler. If applicable,

disconnect the AC power plug prior to cleaning.

Rev B

14.4V 3350mAh

Operating temperature:

•Charge: 10 - 45°C

•Discharge: -20 - 6O°C

Storage temperature:

Recommended 25+/-5°C at 50% state of charge

•Do not immerse the AirPrep Cub Sampler in liquid. Exposure to liquid may damage the battery or the circuits. As a result,

the battery may generate heat, smoke, catch fire, or explode.

•Do not use or place the AirPrep Cub Sampler near an open flame, heater, or high temperature (above 80°C).

•Do not use unauthorized chargers. Use of an unauthorized charger could cause the battery to generate heat, smoke, catch

fire, or explode.

5AIRPREP CUB SAMPLER-MODEL ACD210 BATTERY SAFETY INFORMATION

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6SPECIFICATIONS

Visit this link for AirPrep Bobcat and AirPrep Cub Sampler Specifications

Please contact InnovaPrep at customerservice@innovaprep.com, or an official InnovaPrep distributor for technical

assistance and troubleshooting.

InnovaPrep

www.innovaprep.com

132 E. Main Street, Drexel, MO 64742

Phone: +1 (833) 833-3763

General Inquiries: info@innovaprep.com

Sales: [email protected]

Customer Service: customerservice@innovaprep.com

Caution: Any changes, or modifications not expressly approved by InnovaPrep could void the user’s authority to operate the

equipment.

Note: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of

the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is

operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not

installed and used in accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required

to correct the interference at his own expense.

FOR RESEARCH USE ONLY

InnovaPrep, AirPrep, FluidPrep, CP Select, Concentrating Pipette, Wet Foam Elution are trademarks or registered trademarks

of InnovaPrep LLC. All other brands and names contained herein are the property of their respective owners.

InnovaPrep products are patent protected https://www.innovaprep.com/patents

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