InnovaPrep ACD-200 Manual de usuario

Copyright InnovaPrep LLC www.innovaprep.com 2 
 
 
 
Table of Content 
 
1Product Overview...............................................................3 
 Product Description.............................................................3
 Applications........................................................................4
 Materials Provided..............................................................4
 Materials Required But Not Provided...................................5
 Battery Power.....................................................................5
2ACD-200 Bobcat Method of Operation ................................6 
 Sample Collection on an Electret Filter.................................6 
 Wet Foam Elution................................................................6
 Foam Generation ................................................................7
3Components of the ACD-200 Bobcat ...................................9 
 Description of Components .................................................9
 Check Status Button and Associated LEDs ..........................10 
4Procedures 
Setting Up the ACD-200 Bobcat for Collection ..........................11 
 Optional Battery Operation ...............................................12 
 Installing the Filter ............................................................13 
 Collecting a Sample ...........................................................14 
 Eluting a Filter Sample.......................................................14 
 Cleaning the System..........................................................15 
 Battery Charging ...............................................................15 
 
 
 

1PRODUCT OVERVIEW

This section contains a product description of the InnovaPrep ACD-200

Bobcat™Dry Filter Air Sampler and a brief look at its applications.

PRODUCT DESCRIPTION

The InnovaPrep ACD-200 Bobcat, shown in Figure

1.1, is a lightweight, portable, dry filter air sampler

with a unique patented rapid filter elution system.

The Bobcat aerosol collector takes up little more

than one-quarter of a cubic foot, has an internal

battery, built-in tripod, and can run continuously at

200 liters per minute for up to 12 hours. The system

has a built-in, omni-directional aerosol inlet, easy to

read status display, and a built-in handle. The

system is equipped with a mass flow sensor that

allows for consistent sampling rates throughout the

collection cycle. It can be operated in four

programmable run modes that allow the user to balance collection rate

with battery life. The system is easy to use and operators can be trained

in a little as 10 minutes. Installation of the filter cassettes and the

controls can be manipulated with gloved hands, while wearing personal

protective equipment such as military-style nuclear/biological/chemical

(NBC) protective suits.

Following aerosol collection, the user snaps the sample cup onto the

filter to remove it from the collector, then snaps the elutor onto the

filter. To extract the captured particles from the filter, a canister

containing the elution fluid is pressed into the elutor. The elution fluid is

released from the canister and turns to foam as it flushes the filter. The

wet foam passes through the interstitial spaces of the filter to efficiently

extract captured particles. Sample elution takes approximately 5

seconds and produces 6 to 7 milliliters of liquid sample. The foam

quickly collapses back to a liquid in the sample cup, making it available

for sample processing and analysis.

FIGURE 1.1 ACD-200

BOBCAT

APPLICATIONS

The InnovaPrep ACD-200 Bobcat is ideally suited for the collection of

bioaerosols and particulate matter, including sub-micron sized particles,

airborne molecular contamination, and particulates. This system has

been developed to address a broad range of air sampling requirements.

The filter and elution kit are single use, therefore there is no

requirement for decontamination between samples. Since no liquids

are used in the collector, the system can be operated at temperatures

ranging from -20° C to +60° C in non-condensing environments.

MATERIALS PROVIDED

Inspect each component when unpacking. If damage is observed, do

not use the instrument, and contact InnovaPrep immediately. Remove

any protective packaging that may be present around the instrument.

Examine the packaging material to be sure that materials that have

been provided (see the list below) are removed. Please keep the

custom foam packaging insert for use in the event that a return

shipment is necessary.

Component

InnovaPrep

P/N

Quantity

InnovaPrep ACD-200 Bobcat

ACD-200

External Power Supply

AC01057

Power Cord

HC03019

Power/Trigger Cable Assembly

AC01129

MATERIALS REQUIRED BUT NOT PROVIDED

An AirPrep Filter and Elution Kit is required for sampling. Users have a

choice of two kits with different fluid types.

•AirPrep Filter and Elution Kit - TRIS - Item AC08100T

oRecommended for molecular analysis methods.

•AirPrep Filter and Elution Kit - PBS - Item AC08100P

oRecommended for culture-based analysis methods.

Each AirPrep & Filter Elution Kit includes: a filter, sample cup, elutor

cap, and elution fluid canister. These are available for purchase on

the InnovaPrep website at innovaprep.com.

BATTERY POWER

Battery is not included. Two rechargeable battery options are available

for the ACD-200 Bobcat.

•7.5 Ah Lithium Ion –Recommended for holding the longest

charge (up to 12 hours on continuous mode).

Item AC01054

•4.8 Ah Nickel Metal Hydride –Holds charge up to 7 hours on

continuous mode. Item AC09006

2ACD-200 BOBCAT METHOD OF OPERATION

The InnovaPrep ACD-200 Bobcat uses an electret filter to capture

particles onto the surface of polymer fibers in the filter. A novel Wet

Foam Elution™process is then employed to wash the particles off of the

fiber surfaces into very small liquid volumes.

SAMPLE COLLECTION ON AN ELECTRET FILTER

The system uses a dry 52 mm electret filter as the collection media.

Electret filters are made with a combination of positively charged and

negatively charged fibers. This substantially increases the collection

efficiency of the filter and allows for the use of lower pressure drop

filters, which, in turn, allows for higher sampling rates for extended

periods using battery power. Additional advantages include low

consumable costs, ease of use, and high durability. It has the ability to

operate below 0°C —a limitation for most collectors.

WET FOAM ELUTION

The single-use kit comes with everything you need to recover a fluid

sample, including the Filter, a Sample Cup with Lid, an Elutor, and a

canister of Elution Fluid. The patented Wet Foam Elution process (US

patents https://www.innovaprep.com/patents) is much more efficient

than liquid rinsing for the following reasons:

•Volume Expansion

When rinsing a filter with liquid, most of the liquid volume is

used to fill the dead space inside the filter housing; only a small

portion of the fluid is in contact with the filter surface. This can

be minimized to an extent by reducing the cross-sectional area

of the fluid path across the filter, but a large portion of the

liquid is still underutilized. However, foam is 80-90% gas, which

fills the empty space without contributing to the final sample

volume.

•Increased Viscosity

Liquid has a tendency toward “channeling” when flowing

through a filter, that is, there is an area of high flow in the

center of the fluid path while the portion of flow in contact with

the filter surface is much slower. The higher viscosity of foam

prevents channeling and creates a more uniform flow through

the interstitial spaces of the filter.

•Bubble Dynamics

The micro-bubbles in the foam behave as deformable solids. As

they travel through the filter they move as a rigid body with a

narrow lubricating layer, effectively squeegeeing the particles

off of the surfaces of the filter fibers.

•Exfoliating Action

As the micro-bubbles in the foam impact against each other and

burst, the turbulence and energy produced helps to lift particles

that are adhering to the filter fibers.

•Buffered Elution

The buffered elution fluid is conductive and breaks down the

electrostatic charges holding the particles to the electret filter,

effectively releasing them in the liquid sample.

FOAM GENERATION

The Wet Foam Elution process uses a very specific high-quality foam to

elute the filter. The elution fluid is composed of water, a low

concentration surfactant (0.15% Tween 20), and a pH buffer (Tris or PBS).

This solution is carbonated at 130 psi, which dissolves significant amount

of CO2 into the fluid. During the elution process, the valve opens as the

elution canister is pushed into the elutor that is fitted onto the Filter,

forcing the elution fluid through the pressure orifice, through the filter

and into the Sample Cup. As the fluid passes from the high-pressure

environment on one side of the orifice, to the low-pressure environment

on the other side, the dissolved CO2 expands, comes out of the solution

to form micro-bubbles. These micro-bubbles increase the volume of the

fluid six-fold or more as it passes through the filter. The foam

immediately collapses back into a liquid and is ready for sample

processing an analysis.

3COMPONENTS OF THE ACD-200 BOBCAT

The following section describes the components of the InnovaPrep ACD-

200 Bobcat air sampler.

DESCRIPTION OF COMPONENTS

Description of the InnovaPrep ACD-200 Bobcat components listed in

Figure 3.1, clockwise from top:

•Lid Release –Used to open the ACD-200 for loading or

recovering a filter cartridge.

•Power and Trigger Port - Inlet for the included Power and for

the optional Trigger Connector.

Lid Release

Carrying Handle

Leg Hinges with Locking Push

Pins

Telescoping Tripod Legs

Power and Trigger Port

Mode Selection Knob

Check Status Button

Status Lights

Inlet Cap

Battery Cover

Leg Latches

FIGURE 3.1 COMPONENTS OF THE ACD-200 BOBCAT

•Leg Hinges with Locking Push Pins –Releasing the push pins

allows the user to fold the legs up against the unit for compact

storage and convenient transportation.

•Telescoping Tripod Legs –Each of the three legs consist of three

segments that allow the user to raise and lower the unit to the

desired height.

•Leg Latches –Each leg has three leg latches that allow the user

to telescope the legs to the desired length.

•Battery Cover –Located on the bottom of the unit, the battery

cover holds the battery in place during operation.

•Check Status Button –Used to verify the status of the system.

•Status Lights –Green LED indicates the system is okay, red LEDs

indicate if the lid is open, a filter is needed, the flow rate is

below desired level, or the battery power is low.

•Mode Selection Knob –Rotary knob for selecting the operating

mode and turning the unit off.

•Inlet Cap –Protect from water ingress and is designed as part of

the omni-directional inlet.

CHECK STATUS BUTTON AND ASSOCIATED LEDS

The ACD-200 is equipped with five LEDs that

communicate the status of the unit to the user.

The appropriate LEDs are illuminated when the

Check Status button is pushed. The red Check Lid

LED is illuminated when the lid is open. The red

Check Filter LED is illuminated if there is not a

filter cassette present in the sampler. The

Flowrate LED is illuminated when the internal

mass flow sensor is measuring a flow rate less

than what has been selected. This may be due to an overloaded filter or

a blockage at the exhaust vents located on the bottom of the unit. The

Battery LED is illuminated when the battery needs to be recharged.

FIGURE 3.2 CHECK STATUS

BUTTON AND LEDs

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