Implen NanoPhotometer NP80 Manual de usuario
NanoPhotometer NP80/N60/N50/C40
User Manual
Version 1.3.2
Software Version 1.1.11648
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
2
Declaration of conformity for the NanoPhotometer (C40/N50/N60/NP80)
This is to certify that the Implen NanoPhotometer conforms to the requirements of the following
directives:
2014/35/EU Low Voltage Equipment Safety Directive
2004/108/EC EMC Directive
IEC 60529 Protection class IP20
2002/95/EC Restrictions on the use of certain Hazardous Substances
in Electrical and Electronic Equipment (ROHS)
2012/19/EU EC Directive on Waste Electrical and Electronic Equipment (WEEE)
2003/108/EC & 2008/34/EC. By ensuring this product is disposed of
correctly, you will help prevent potential negative consequences for the
environment and human health, which could otherwise be caused by
inappropriate waste handling of this product.
FCC 47 CFR Part15 §15.107 and §15.109
EN 301 489-1 V1.9.2 Radio and ancillary equipment for portable use (portable equipment); EUT
Operating frequency range: 2.4 –2.4835 GHz
EN 301 489-17 V2.2.1 Electromagnetic compatibility and Radio Spectrum Matters (ERM)
IEC 62133 and UN38.3 Battery certification and transport test
Standards to which conformity is declared, where relevant, are as follows:
IEC/EN 61010-1:2012 Safety requirements for electrical equipment for measurement, control and
laboratory use. General requirements.
EN61326-1:2013 Electromagnetic compatibility- generic emission standard electrical
equipment for measurement, control and laboratory use.
Signed:
Dr. Thomas Sahiri
Managing Director
Implen GmbH
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
3
Contents
1. NANOPHOTOMETER AT A GLANCE.........................................................................6
NANOPHOTOMETER OVERVIEW ................................................................................................6
NANOPHOTOMETER NP80..................................................................................................................7
NANOPHOTOMETER N60 ....................................................................................................................8
NANOPHOTOMETER N50 ....................................................................................................................9
NANOPHOTOMETER C40 ..................................................................................................................10
INSTRUMENT REAR PANEL (NP80/N60/N50/C40)..............................................................................11
INSTRUMENT BOTTOM VIEW (NP80/N60/N50/C40)............................................................................11
ACCESSORIES.......................................................................................................................12
STANDARD ACCESSORIES.................................................................................................................12
OPTIONAL ACCESSORIES..................................................................................................................12
CONNECTIVITY.......................................................................................................................15
NANOPHOTOMETER SPECIFICATIONS......................................................................................16
2. GETTING STARTED..............................................................................................17
SPECTROPHOTOMETER INSTALLATION ....................................................................................17
SOFTWARE INSTALLATION......................................................................................................19
NPOS OVERVIEW.............................................................................................................................19
REQUIREMENTS AND COMPATIBILITY.................................................................................................19
INSTALLING SOFTWARE ON COMPUTER..............................................................................................20
INSTALLING NANOPHOTOMETER APP ON TABLET OR SMARTPHONE....................................................20
FIRST STEPS AND CONFIGURATION WIZARD........................................................................................21
PRINTER INSTALLATION ....................................................................................................................21
3. NANOPHOTOMETER BASICS................................................................................22
APPLICATIONS OVERVIEW......................................................................................................22
ICONS...................................................................................................................................24
BUTTONS..............................................................................................................................25
SIDE TAB BAR ..................................................................................................................................26
DATA PROCESSING DIALOGS.............................................................................................................28
BASIC OPERATION.................................................................................................................31
NANOVOLUME MEASUREMENT BASICS (N50/N60/NP80)...................................................................31
CUVETTE MEASUREMENT BASICS (C40/NP80)..................................................................................32
SUBMICROLITER CELL MEASUREMENTS BASICS (C40).......................................................................33
SAMPLE HANDLING TIPS...................................................................................................................35
BATTERY OPERATION............................................................................................................37
4. NANOPHOTOMETER APPLICATIONS.....................................................................38
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
4
NUCLEIC ACIDS ..................................................................................................................38
METHOD OVERVIEW..........................................................................................................................38
MEASUREMENT PROTOCOL...............................................................................................................38
CALCULATIONS ................................................................................................................................40
PROTEIN UV ......................................................................................................................45
METHOD OVERVIEW..........................................................................................................................45
MEASUREMENT PROTOCOL...............................................................................................................46
CALCULATIONS ................................................................................................................................47
PROTEIN ASSAYS ...............................................................................................................51
METHOD OVERVIEW..........................................................................................................................51
MEASUREMENT PROTOCOL...............................................................................................................52
CALCULATIONS ................................................................................................................................54
KINETICS ..........................................................................................................................54
METHOD OVERVIEW..........................................................................................................................54
MEASUREMENT PROTOCOL...............................................................................................................55
CALCULATIONS ................................................................................................................................56
OD600 ..........................................................................................................................56
METHOD OVERVIEW..........................................................................................................................56
MEASUREMENT PROTOCOL...............................................................................................................57
CALCULATIONS ................................................................................................................................58
MORE APPS .......................................................................................................................58
MORE APPS:WAVELENGTH ................................................................................................59
METHOD OVERVIEW..........................................................................................................................59
MEASUREMENT PROTOCOL...............................................................................................................59
CALCULATIONS ................................................................................................................................60
MORE APPS:WAVESCAN ...................................................................................................61
METHOD OVERVIEW..........................................................................................................................61
MEASUREMENT PROTOCOL...............................................................................................................61
CALCULATIONS ................................................................................................................................62
MORE APPS:ABSORBANCE RATIO .....................................................................................62
METHOD OVERVIEW..........................................................................................................................62
MEASUREMENT PROTOCOL...............................................................................................................62
CALCULATIONS ................................................................................................................................64
MORE APPS:CONCENTRATION ...........................................................................................64
METHOD OVERVIEW..........................................................................................................................64
MEASUREMENTS PROTOCOL.............................................................................................................64
CALCULATIONS ................................................................................................................................66
MORE APPS:STANDARD CURVE ........................................................................................66
METHOD OVERVIEW..........................................................................................................................66
MEASUREMENTS PROTOCOL.............................................................................................................66
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
5
CALCULATIONS ................................................................................................................................68
CUSTOM APPS ......................................................................................................................68
STORED RESULTS ..............................................................................................................68
STORED METHODS .............................................................................................................69
5. PREFERENCES....................................................................................................69
GENERAL..............................................................................................................................69
DATE AND TIME................................................................................................................................69
DISPLAY ..........................................................................................................................................70
ABOUT.............................................................................................................................................70
WIFI ACCESS POINT.........................................................................................................................70
ILLUMINATION SAMPLE WINDOW .......................................................................................................70
ENABLE NANOVOLUME.....................................................................................................................70
DYES 70
6. TROUBLESHOOTING............................................................................................71
SELF-CALIBRATION TEST ........................................................................................................71
7. ASSISTANCE.......................................................................................................71
SUPPORT..............................................................................................................................71
REPORT PROBLEM ................................................................................................................72
SOFTWARE MAINTENANCE .....................................................................................................72
RESET .............................................................................................................................................72
SOFTWARE UPDATE..........................................................................................................................72
CONTACT IMPLEN..................................................................................................................73
8. MAINTENANCE....................................................................................................74
MAINTENANCE FREE TECHNOLOGY.........................................................................................74
REPLACEMENT PARTS ...........................................................................................................74
CLEANING AND GENERAL CARE .............................................................................................75
9. WARRANTY.........................................................................................................75
10. ALPHABETICAL APPENDIX...................................................................................76
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
6
1.NANOPHOTOMETER AT A GLANCE
NANOPHOTOMETER OVERVIEW
The Implen NanoPhotometer spectrophotometer is a mobile, simple to use UV/Visible instrument with
a CCD array detector with options ranging from nanovolume to standard cuvette and all-in-one
solutions. The N50, N60, and NP80 spectrophotometers measure sample volumes ranging from 0.3 -
2 µl. The NP80 model also has the capability to measure samples using a standard cuvette. The C40
is a mobile standard cuvette solution with the option to upgrade to accommodate nanovolume
measurements.
The NanoPhotometer runs on a Linux based operating system (NPOS) that is designed for the use of
pre-programmed and custom applications with a high degree of flexibility and processing power.
Sample Compression Technology™provides easy sample handling which is independent of surface
tension. This technology squeezes the sample between two quartz surfaces allowing for unmatched
precision and accuracy without the need for dilutions. Combined with our True Path Technology™ the
system offers lifetime accuracy and precision without the need for maintenance or recalibration.
Note: It is recommended to use a properly calibrated pipette with high quality tips to ensure delivery
of appropriate sample volumes for nanovolume sample applications.
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
7
NANOPHOTOMETER NP80
Item Number
Touchscreen
Battery Pack
NP80
-
-
NP80-Touch
+
-
NP80-Mobile
+
+
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
8
NANOPHOTOMETER N60
Item Number
Touchscreen
Battery Pack
N60
-
-
N60-Touch
+
-
N60-Mobile
+
+
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
9
NANOPHOTOMETER N50
Item Number
Touchscreen
Battery Pack
N50
-
-
N50-Touch
+
-
NanoPhotometer NP80/N60/N50/C40 User Guide Version 1.3.2
10
NANOPHOTOMETER C40
Item Number
Touchscreen
Battery Pack
C40
-
-
C40-Touch
+
-
C40-Mobile
+
+
Otros manuales para NanoPhotometer NP80
1
Este manual sirve para los siguientes modelos
3
Tabla de contenidos
Otros manuales de Instrumento de medición de Implen
Manuales populares de Instrumento de medición de otras marcas
Endress+Hauser
Endress+Hauser Proline Promag 50 Especificaciones técnicas
Siemens
Siemens SITRANS F Coriolis FCT030 Manual de lista de piezas
KLINGER
KLINGER CMF V Series Manual de usuario
EXFO
EXFO FTB-2 Manual de operación y mantenimiento
Keysight
Keysight M8290A Manual de usuario
ADTEK
ADTEK MW-5 Manual de usuario