Harvard Bioscience Warner OC-725D Manual de usuario

Publication 5720-001-REV 1.2

Oocyte Clamp

Ampliﬁer

User’s Manual

Warner Instruments OC-725D

www.warneronline.com

Table of Contents

SUBJECT

Warranty

Safety

Introducon

Unique Features

High Voltage Compliance

Bath Clamp Headstage

Voltage Headstage Probe

Voltage and Current Meters

Addional Features

Buzz controls

Electrode Test

Overload Alarm



Nomenclature



Control Descripon

Front panel

Voltage electrode

Bath electrodes



Commands

Current electrode

Rear panel

Addional components

Voltage recording headstages

Bath headstage

Current electrode cable

Model cell

Comments



High voltage outputs

Using the Model Membrane

PAGE #

6–7

9–15

16–19

Table of Contents

PAGE #

20–26

26–29

30–31

SUBJECT

Inial instrument sengs

Test procedures



Voltage electrode test

Buzz

Current electrode test

DC clamp test

AC clamp test

Setup



Electrode holders

Bath probe

Electrode placement and grounding

Bath clamp electrode placement





Using the gain select



A Procedure for Recording from Oocytes



Voltage electrode placement

Current electrode placement

Impaling the cell

Clamping the cell

Clamping high conductance cells

Unclamping the cell

Removing the electrodes

Comments and Recommendaons

Membrane damage

Repeated recordings

Electrophysiology



www.warneronline.com

Table of Contents

PAGE #

32–35

SUBJECT

Appendix



laboratory environment

Gain telegraph outputs







4

Serial Numbers





Calibraons



Warranty











This warranty extends only to the original customer purchaser.

IN NO EVENT SHALL WARNER INSTRUMENTS BE LIABLE FOR INCIDENTAL OR































Repair Facilies and Parts







CAUTION

This instrument is not registered with the FDA and is not for clinical

use on human paents.

Warranty & Repair Information

www.warneronline.com

Safety Information







To Prevent Hazard or Injury

Use Proper Line Cord





Ground the Product







product is properly grounded.

Make Proper Connecons



to the unit must be no longer than 3 meters.

Observe All Terminal Rangs



Use Proper Fuse



Avoid Exposed Circuitry



Do Not Operate with Suspected Failures





Orient the Equipment Properly





Place Product in Proper Environment



Observe all Warning Labels on Product

Read all labels on product to ensure proper usage.

CAUTION

Refer to Manual

Protecve

Ground Terminal

CAUTION: FOR RESEARCH USE ONLY.

NOT FOR CLINICAL USE ON PATIENTS.

6

Introduction







Unique Features

High Voltage Compliance:



6may be













Bath Clamp Headstage:













Voltage Headstage Probe: 





Voltage and Current Meters:

membrane voltage Vmand membrane current Im

e



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Introduction

Addional Features

Buzz controls



Electrode Test

Overload Alarm 



DC Offsets

8

Nomenclature

Text Convenons











• Warner Instrument product numbers are presented using a bold type.



.





italic type.

slow, fast, 100 mV

• Special comments and warnings are presented in highlighted text.



THIS EQUIPMENT IS NOT DESIGNED NOR INTENDED FOR USE ON HUMAN SUBJECTS

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Control Description













and the current cable with electrode holder.

Front Panel



[Vm [Im. The instrument

 and Ve

on/off and 

Voltage Electrode

The   the

m and 

the Vm.

The  reports the

m



The  



voltage probe to the instrument.

The Vm



at the 



The Vm control is used





Ω

the meter or reported at the Vmx10 output BNC

Ω

The Vm



The Vmx10 

Tabla de contenidos

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