Hanna Instruments PH 209 Manual de usuario

Instruction Manual

www.hannainst.com

pH 209 • pH 209R

Bench Top pH Meters

pH 209 bench-top pH meter is designed for simplicity of use.

It features a large easy-to-read liquid crystal display (LCD) and user

friendly keyboard.

The pH calibration is made simple through the easy-to-operate front

panel knobs for offset and slope adjustment.

A front knob is provided for easy manual temperature compensation of

the pH reading.

pH 209 can also measure ion concentration (ISE) and ORP (Oxidation

Reduction Potential) in the mV range.

The range selection (pH, mV or °C for temperature compensation) is

made simple through the front membrane keyboard.

The large LCD is provided with arrows that quickly and clearly indicate

the selected range.

It is also available the model pH209R with recorder output feature.

GENERAL DESCRIPTION

PRELIMINARY EXAMINATION

Remove the instrument from the packing material and examine it

carefully to make sure that no damage has occurred during shipping.

If there is any damage, notify your dealer.

Each meter is supplied complete with:

• HI 1332B plastic-body combination double-junction refillable

pH electrode with BNC connector and 1m (3.3') cable

•12 Vdc adapter

•Instruction manual.

Note: Save all packing material until you are sure that the instru-

ment functions correctly. All defective items must be returned in

the original packing with the supplied accessories.

Dear Customer,

Thank you for choosing a Hanna Instruments Product.

Please read this instruction manual carefully before using the instru-

ment.

This manual will provide you with all the necessary information for the

correct use of the instrument, as well as a precise idea of its versatility

in a wide range of applications.

If you need additional technical information, do not hesitate to e-mail

us at [email protected].

These instruments are in compliance with the directives.

PRELIMINARY EXAMINATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

GENERAL DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

FUNCTIONAL DESCRIPTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

SPECIFICATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

OPERATIONAL GUIDE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

PH CALIBRATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

PH VALUES AT VARIOUS TEMPERATURES . . . . . . . . . . . . . . . . . . . .10

ELECTRODE CONDITIONING & MAINTENANCE . . . . . . . . . . . . . . . . . 11

TEMPERATURE-RESISTANCE CORRELATION

FOR HANNA pH SENSITIVE GLASS . . . . . . . . . . . . . . . . . . . . . . . . 14

ACCESSORIES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

ELECTRODE APPLICATION REFERENCE GUIDE . . . . . . . . . . . . . . . . . 21

WARRANTY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

CE DECLARATION OF CONFORMITY . . . . . . . . . . . . . . . . . . . . . . . . 23

TABLE OF CONTENTS

FUNCTIONAL DESCRIPTION

Range 0.00 to 14.00 pH

±1999 mV

Resolution 0.01 pH / 1 mV

Accuracy (@20°C) ±0.01 pH / ±1 mV

Typical EMC Deviation ±0.03 pH / ±2 mV

pH Calibration Manual, 2 point, through knobs

Temperature Compensation

Manual from 0 to 100°C (32 to 212°F)

pH Electrode (included)

HI 1332B plastic body, double junction,

refillable, with BNC and 1m (3.3') cable

Analog output 0 to 5V

(pH209R only) 0 to 14 pH / -1999 to +1999 mV

Temperature: always 0

Input Impedance 1012 ohm

Power Supply 12 Vdc adapter (included)

Environment 0 to 50°C (32 to 122°F);

RH max 95% non-condensing

Dimensions 240 x 182 x 74 mm (9.4x7.2x2.9")

Weight approx. 1.0 kg (2.2 lb.)

SPECIFICATIONS

1) Liquid Crystal Display (LCD)

2) pH slope adjustment knob

3) pH offset adjustment knob

4) pH range selection key

5) mV range selection key

6) °C range selection key

7) Temperature setting knob

8) ON/OFF switch

9) Power adapter socket

10) BNC electrode connector

11) Electrode reference socket

12) Analog output (PH209R only)

4 cm

(1½")

• Press the pH key to display the pH measurement.

• The display shows the pH value of the test solution compensated

for temperature.

If measurements are taken in different samples successively, it is

recommended that the electrode be rinsed thoroughly for better condi-

tioning and to eliminate cross-contamination of the sample.

For the rinsing process, it is recommended to use a liberal amount of

the next solution to be measured.

TAKING ORP MEASUREMENTS

pH 209 has the capability to take ORP measurements, using an ORP

electrode. Hanna Instruments offers a variety of ORP electrodes for this

purpose (see accessories). Contact your Dealer for more information.

• Connect the ORP electrode to the meter and

submerge the tip (4 cm / 1½") into the sample

to be tested.

Note: ORP measurements are taken without temperature compensa-

tion.

• Press the mV key to enter the mV mode. Allow a few

minutes for the reading to stabilize.

• The display will indicate the mV value (positive or negative).

AFTER MEASUREMENTS

• Press the ON/OFF switch again to switch the instrument off.

Power connection

Plug the supplied 12 Vdc adapter into the power supply socket (#9

on page 4).

Note: Make sure the mains line is protected by a fuse.

Electrode connection

For combination pH or ORP electrodes (with internal reference)

connect the electrode's BNC to the socket provided (#10 on page 4).

For an electrode with a separate reference, connect the measuring

electrode's BNC to the BNC socket (#10 on page 4) and the reference

electrode's jack to the socket provided (#11 on page 4).

Note: to prevent damage to the electrode, remove the pH electrode

from the solution before turning the meter off.

If the meter is OFF, detach the electrode from the meter before

immersing the electrode in the storage solution.

TAKING pH MEASUREMENTS

Make sure that the instrument has been calibrated for pH before

taking pH measurements.

• Switch the instrument on by pressing the ON/OFF

switch.

• Immerse the electrode tip (4 cm/1½") into the

sample and shake briefly.

• Take the temperature of the solution with a

ChecktempC or a glass thermometer (e.g.25°C).

• Press the °C key to display temperature setting on the LCD and

adjust the temperature knob to display the temperature of the

sample.

4 cm

(1½")

OPERATIONAL GUIDE

HI 7004 HI 7007

HI 7004

IMPORTANT

The instrument's pH range should be re-calibrated:

• When the meter is new.

• Whenever the pH electrode is replaced.

• At least once a month.

• After use in aggressive chemicals.

• After cleaning procedure and changing the reference electrolyte.

• For greatest accuracy.

INITIAL PREPARATION

Pour small quantities (up to 4cm / 1½" level)

of pH 7.01 (HI 7007) and pH4.01 (HI 7004)

or pH 10.01 (HI 7010) solution into clean

beakers. If possible use plastic beakers to

minimize any EMC interferences.

If you are measuring in the acid range, use pH 4.01 as second

buffer; if you are measuring in the alkaline range, use pH 10.01 as

second buffer.

For accurate calibration, use two bea-

kers for each buffer solution; the first

for rinsing the electrode, the second

for calibration. In this way, contami-

nation of the buffer is minimized.

Note: The electrode should be submerged approximately 4 cm (1½")

into the solution.

Note: If you need to calibrate to NBS standards, use pH 6.86 (HI

7006) and pH 9.18 (HI 7009) instead of pH 7.01 and 10.01

respectively.

Use a ChecktempC or a glass thermometer as reference.

PROCEDURE

• Switch the instrument on by pressing the

ON/OFF button.

• Rinse and immerse the pH electrode into

pH 7.01 buffer and shake briefly. Wait for

the reading to stabilize.

pH CALIBRATION • Note the temperature of the buffer solution using

a ChecktempC or a glass thermometer

(e.g.20°C).

• Press the °C key to select temperature setting.

• Adjust the Temperature knob until the LCD shows the noted

temperature.

• Press the pH key to select pH measurement.

• Wait a couple of minutes and adjust the OFFSET knob until

display shows the pH value at the noted temperature (see the pH

versus temperature chart).

• Rinse and immerse the pH electrode in pH 4.01/pH 10.01 buffer

and shake briefly.

• Wait a couple of minutes and adjust the SLOPE knob until

display shows the pH value at the noted temperature (see the pH

versus temperature chart).

• The pH calibration is now complete.

4 cm

(1½")

HI 7007

RINSE

HI 7007

CALIBRATION

Reference

Filling Hole

Reference

Filling Hole

Sensitive

Wire

Reference

Wire

Reference

Junction

Glass

Bulb

Glass

Bulb

Reference

Junction

Reference

Junction Reference

Junction

Platinum or

Gold tip Platinum or

Gold tip

Reference

Wire

Reference

Wire

Sensitive

Wire

Glass Body

pH Electrode

Plastic Body

pH Electrode

Plastic Body

ORP Electrode Glass Body

ORP Electrode

Reference

Wire

ELECTRODE CONDITIONING

AND MAINTENANCE

PREPARATION

Remove the protective cap.

DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT. This is normal

with electrodes and they will disappear when rinsed with water.

During transport tiny bubbles of air may form inside the glass bulb

affecting proper functioning of the electrode. These bubbles can be

removed by "shaking down" the electrode as you would do with a

glass thermometer.

If the bulb and/or junction is dry, soak the electrode in HI 70300

storage solution for at least one hour.

pH VALUES AT VARIOUS TEMPERATURES

Temperature has an effect on pH. The calibration buffer solutions are

affected by temperature changes to a lesser degree than normal

solutions.

For manual temperature calibration please refer to the following chart.

For instance, if the buffer temperature is 25°C, the display should

show pH 4.01, 7.01 or 10.01 at pH 4, 7 or 10 buffers, respectively.

At 20°C, the display should show pH 4.00, 7.03 or 10.06. The meter

reading at 50°C will then be 4.06, 6.98 or 9.82.

Note: to prevent damage to the electrode, remove the pH electrode

from the solution before turning the meter off.

If the meter is OFF, detach the electrode from the meter before

immersing the electrode in the storage solution.

TEMP pH VALUES

°C °F 4.01 6.86 7.01 9.18 10.01

104

113

122

131

140

149

158

4.01

4.00

4.01

4.02

4.03

4.04

4.05

4.06

4.07

4.09

4.11

4.12

6.98

6.95

6.92

6.90

6.88

6.86

6.85

6.84

6.83

6.84

6.85

7.13

7.10

7.07

7.04

7.03

7.01

7.00

6.99

6.98

6.99

9.46

9.39

9.33

9.27

9.22

9.18

9.14

9.10

9.07

9.04

9.01

8.99

8.97

8.95

8.93

10.32

10.24

10.18

10.12

10.06

10.01

9.96

9.92

9.88

9.85

9.82

9.79

9.77

9.76

9.75

Follow the Storage Procedure above.

CLEANING PROCEDURE

General Soak in HI 7061 general cleaning solution for approxi-

mately 30 minutes.

Removal of films, dirt or deposits on the membrane/junction:

- Protein Soak in HI 7073 protein cleaning solution for 15 min.

- Inorganic Soak in HI 7074 inorganic cleaning solution for 15

minutes.

- Oil/grease Rinse with HI 7077 Oil & Fat cleaning solution.

IMPORTANT: After performing any of the cleaning procedures rinse

the electrode thoroughly with distilled water, refill the reference cham-

ber with fresh electrolyte (not necessary for gel-filled electrodes) and

soak the electrode in HI 70300 storage solution for at least 1 hour

before taking measurements.

TROUBLESHOOTING

Evaluate your electrode performance based on the following.

•Noise (Readings fluctuate up and down) could be due to:

– Clogged/Dirty Junction: Refer to the Cleaning Procedure

above.

– Loss of shielding due to low electrolyte level (in refillable

electrodes only): refill with fresh HI 7071 for single junction or

HI 7082 for double junction electrodes.

•Dry Membrane/Junction: soak in HI 70300 storage solution for

at least 1 hour.

•Drifting: soak the electrode tip in warm (approx. 50-60°C)

Hanna HI 7082 solution for one hour and rinse the tip with

distilled water. Refill with fresh HI 7071 for single junction

electrodes and HI 7082 for double junction electrodes (refillable

electrodes only).

•Low Slope: refer to the cleaning procedure above.

•No Slope: check the electrode for cracks in glass stem or bulb

and replace the electrode.

•Slow Response/Excessive Drift: soak the tip in HI 7061

solution for 30 minutes, rinse thoroughly in distilled water and

then follow the Cleaning Procedure above.

For refillable electrodes:

If the filling solution (electrolyte) is more than 2½ cm (1") below the

fill hole, add HI 7082 3.5M KCl electrolyte solution for double junction

or HI 7071 3.5M KCl+AgCl electrolyte solution for single junction

electrodes.

For a faster response, unscrew the fill hole screw during measure-

ments.

For AmpHel®

electrodes:

If the electrode does not respond to pH changes, the battery is run

down and the electrode should be replaced.

MEASUREMENT

Rinse the electrode tip with distilled water. Immerse the tip (bottom 4

cm /1½") in the sample and stir gently for a few seconds.

For a faster response and to avoid cross contamination of the samples,

rinse the electrode tip with a few drops of the solution to be tested,

before taking measurements.

STORAGE

To minimize clogging and assure a quick response time, the glass bulb

and the junction should be kept moist and not allowed to dry out.

Replace the protective cap with a few drops of HI 70300 storage

solution or, in its absence, electrolyte solution (HI 7071 for single

junction, or HI 7082 for double junction electrodes). Follow the

Preparation Procedure above before taking measurements.

NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER.

PERIODIC MAINTENANCE

Inspect electrode and cable. The cable used for connection to the

meter must be intact and there must be no points of broken

insulation on the cable or cracks on the electrode stem or bulb.

Connectors must be perfectly clean and dry. If any scratches or cracks

are present, replace the electrode. Rinse off any salt deposits.

For refillable electrodes:

Refill the reference chamber with fresh electrolyte (HI 7071 for single

junction or HI 7082 for double junction electrodes). Allow the electrode

to stand upright for 1 hour.

ACCESSORIES

pH CALIBRATION SOLUTIONS

HI 70004P pH 4.01 buffer solution, 20 mL sachet, 25 pcs

HI 70007P pH 7.01 buffer solution, 20 mL sachet, 25 pcs

HI 70010P pH 10.01 buffer solution, 20 mL sachet, 25 pcs

HI 7004L pH 4.01 buffer solution, 500 mL

HI 7006L pH 6.86 buffer solution, 500 mL

HI 7007L pH 7.01 buffer solution, 500 mL

HI 7009L pH 9.18 buffer solution, 500 mL

HI 7010L pH 10.01 buffer solution, 500 mL

HI 8004L pH 4.01 buffer solution in FDA bottle, 500 mL

HI 8006L pH 6.86 buffer solution in FDA bottle, 500 mL

HI 8007L pH 7.01 buffer solution in FDA bottle, 500 mL

HI 8009L pH 9.18 buffer solution in FDA bottle, 500 mL

HI 8010L pH 10.01 buffer solution in FDA bottle, 500 mL

ELECTRODE STORAGE SOLUTION

HI 70300L Storage solution, 500 mL

HI 80300L Storage solution in FDA bottle, 500 mL

ELECTRODE CLEANING SOLUTIONS

HI 70000P Electrode rinsing solution, 20 mL sachet, 25 pcs

HI 7061L General cleaning solution, 500 mL

HI 7073L Protein cleaning solution, 500 mL

HI 7074L Inorganic cleaning solution, 500 mL

HI 7077L Oil & Fat cleaning solution, 500 mL

HI 8061L General cleaning solution in FDA bottle, 500 mL

HI 8073L Protein cleaning solution in FDA bottle, 500 mL

HI 8077L Oil & Fat Cleaning solution in FDA bottle, 500 mL

ELECTROLYTE SOLUTIONS

HI 7071 3.5M KCl + AgCl electrolyte solution, 4 x 30 mL, for

single junction electrodes

HI 7072 1M KNO3electrolyte solution, 4 x 30 mL

HI 7082 3.5M KCl electrolyte solution, 4 x 30 mL, for double

junction electrodes

HI 8071 3.5M KCl + AgCl electrolyte solution in FDA bottle, 4 x

30 mL, for single junction electrodes

HI 8072 1M KNO3electrolyte solution in FDA bottle, 4 x 30 mL

HI 8082 3.5M KCl electrolyte solution in FDA bottle, 4 x 30 mL,

for double junction electrodes

Alkaline Error

1.0 Mol L-1 Na+

0.1 Mol L-1 Na+

Sodium Ion Correction for the Glass at 20-25°C

Concentration pH Error

13.00

13.50

14.00

12.50

13.00

13.50

14.00

0.10

0.14

0.20

0.10

0.18

0.29

0.40

TEMPERATURE-RESISTANCE CORRELATION

FOR HANNA pH SENSITIVE GLASS

The resistance of glass electrodes partially depends on the temperature.

The lower the temperature, the higher the resistance. It takes longer time

for the reading to stabilize if the resistance is higher. In addition, the

response time will suffer to a greater degree at temperatures below 10°C.

Since the resistance of the pH electrode is in the range of 200 Mohm,

the current across the membrane is in the pico Ampere range. Large

currents can disturb the calibration of the electrode for many hours.

For these reasons high humidity environments, short circuits and

static discharges are detrimental to a stable pH reading.

The pH electrode's life also depends on the temperature. If constantly

used at high temperatures, the electrode life is drastically reduced.

Typical Electrode Life

Ambient Temperature 1- 3 years

90 °C Less than 4 months

120°C Less than 1 month

High concentrations of sodium ions interfere with readings in alkaline

solutions; the pH at which the interference starts to be significant

depends upon the composition of the glass. This interference is the

alkaline error and causes the pH to be underestimated. Hanna's glass

formulations have the indicated characteristics.

-2

-10

Ω

-10

Ω

2x109

1x109

2x108

1x108

2x107

1x107

-20 -10 0 +10 +20+30+40 +50 +60 +70 +80 +90 °C

HI 1330B

Glass body, semimicro, single junction, refillable, combination pH

electrode. Use: laboratory, vials.

HI 1331B

Glass body, semimicro, single junction, refillable, combination pH

electrode. Use: flasks.

HI 1230B

Plastic body, double junction, gel-filled, combination pH electrode.

Use: general, field.

HI 2031B

Glass body, semimicro, conic, refillable, combination pH electrode. Use:

semisolid products.

HI 1332B

Plastic body, double junction, refillable, combination pH electrode.

Use: general purpose.

ORP PRETREATMENT SOLUTIONS

HI 7091L Reducing pretreatment solution, 500 mL

HI 7092L Oxidizing pretreatment solution, 500 mL

pH ELECTRODES

All electrodes part numbers ending in B are supplied with a BNC

connector and 1 m (3.3') cable.

HI 1043B

Glass body, double junction, refillable, combination pH electrode.

Use: strong acid/alkali.

HI 1053B

Glass body, triple ceramic, conic shape, refillable, combination pH

electrode. Use: emulsions.

HI 1083B

Glass body, micro, Viscolene, non-refillable, combination pH electrode.

Use: biotechnology, micro titration.

HI 1131B

Glass body, single junction, refillable, combination pH electrode.

Use: general purpose.

120 mm

4.7"

12 mm

0.5"

9.5mm DIA

0.37"

"S" VERSION

HI 1043

120 mm

4.7"

12 mm

0.5"

"S" VERSION

HI 1053

120 mm

12 mm

0.5" 5 mm

0.2" 3 mm

0.12" 3.0 mm DIA

0.12"

HI 1083

120 mm

4.7"

5mm

0.2"

5mm DIA

0.2"

"S" VERSION

HI 1330

120 mm

4.7"

12 mm

0.5"

VERSION

HI 1230

75 mm

6 mm

0.25"

VERSION

HI 2031

120 mm

4.7"

12 mm

0.5"

"S" VERSION

HI 1332

120 mm

4.7"

12 mm

0.5"

9.5mm DIA

0.37"

"S" VERSION

HI 1131

210 mm

8.25"

8 mm

0.3" 7.5mm DIA

0.29"

"S" VERSION

HI 1331

FC 100B

PVDF body, double junction, refillable, combination pH electrode.

Use: general purpose for food industry.

FC 200B

PVDF body, open junction, conic, Viscolene, non-refillable, combination

pH electrode. Use: meat & cheese.

FC 210B

Glass body, double junction, conic, Viscolene, non-refillable, combina-

tion pH electrode. Use: milk, yogurt.

FC 220B

Glass body, triple-ceramic, single junction, refillable, combination pH

electrode. Use: food processing.

FC 911B

PVDF body, double junction, refillable with built-in amplifier, combi-

nation pH electrode. Use: very high humidity.

120 mm

4.7"

12 mm

0.5"

FC 100

75 mm

2.95"

6 mm

0.25"

FC 200

120 mm

4.7"

12 mm

0.5"

FC 210

120 mm

4.7"

12 mm

0.5"

9.5mm DIA

0.37"

FC 220

HI 1413B

Glass body, single junction, flat tip, Viscolene, non-refillable, combina-

tion pH electrode. Use: surface measurement.

ORP ELECTRODES

HI 3131B

Glass body, refillable, combination platinum ORP electrode.

Use: titration.

HI 3230B

Plastic body, gel-filled, combination platinum ORP electrode.

Use: general purpose.

HI 4430B

Plastic body, gel-filled, combination gold ORP electrode.

Use: general purpose.

110 mm

4.3"

12 mm

0.5"

FC 911

110 mm

4.3"

12 mm

0.5"

HI 1413

150 mm

5.9"

12 mm

0.5"

"S" VERSION

HI 3131

120 mm

4.7"

12 mm

0.5"

HI 3230

"S" VERSION

120 mm

4.7"

12 mm

0.5"

HI 4430

"S" VERSION

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