Hanna Instruments C 214 Manual de usuario

Instruction Manual

C 214

Multiparameter Bench

Photometer

for

Wastewater Treatment

Application

www.hannainst.com

These Instruments are in

Compliance with the CE Directives

MANC214R3 10/04

TECHNICAL SERVICE CONTACTS

Australia:

Tel. (03) 9769.0666 • Fax (03) 9769.0699

e-mail: [email protected]

China:

Tel. (10) 88570068 • Fax (10) 88570060

e-mail: [email protected]

Egypt:

Tel. & Fax (02) 2758.683

e-mail: [email protected]

Germany:

Tel. (07851) 9129-0 • Fax (07851) 9129-99

e-mail: [email protected]

Greece:

Tel. (210) 823.5192 • Fax (210) 884.0210

e-mail: [email protected]

Indonesia:

Tel. (21) 4584.2941 • Fax (21) 4584.2942

e-mail: [email protected]

Japan:

Tel. (03) 3258.9565 • Fax (03) 3258.9567

e-mail: [email protected]

Korea:

Tel. (02) 2278.5147 • Fax (02) 2264.1729

e-mail: [email protected]

Malaysia:

Tel. (603) 5638.9940 • Fax (603) 5638.9829

e-mail: [email protected]

Norway:

Tel. (23) 3811.00 • Fax (23) 3811.01

e-mail: [email protected]

Singapore:

Tel. 6296.7118 • Fax 6291.6906

e-mail: [email protected]

South Africa:

Tel. (011) 615.6076 • Fax (011) 615.8582

e-mail: [email protected]

United Kingdom:

Tel. (01525) 850.855 • Fax (01525) 853.668

e-mail: [email protected]

USA:

Tel. (401) 765.7500 • Fax (401) 765.7575

e-mail: [email protected]

For additional Technical Support in your local lan-

guage, see www.hannainst.com

Dear Customer,

Thank you for choosing a Hanna product. Please read this instruction

manual carefully before using the meter. This manual will provide you

with the necessary information for the correct use of the instrument. If

you need additional technical information, do not hesitate to e-mail us

at [email protected]. These instruments are in compliance with

directives.

TABLE OF CONTENTS

PRELIMINARY EXAMINATION ........... 3

ABBREVIATIONS ............................. 3

GENERAL DESCRIPTION ................... 4

SPECIFICATIONS ............................. 5

PRECISION AND ACCURACY ............. 5

PRINCIPLE OF OPERATION ............... 6

FUNCTIONAL DESCRIPTION .............. 8

GUIDE TO DISPLAY CODES .............. 9

TIPS FOR AN ACCURATE

MEASUREMENT ............................12

PARAMETERS REFERENCE TABLES .. 17

HEALTH & SAFETY ........................ 19

OPERATIONAL GUIDE .................... 20

AMMONIA LOW RANGE ................. 22

AMMONIA HIGH RANGE ................ 25

CHLORINE, FREE ........................... 28

CHLORINE, TOTAL .........................31

NITRATE .......................................34

NITROGEN, TOTAL LR ....................37

NITROGEN, TOTAL HR ................... 42

OXYGEN DEMAND, CHEMICAL LR ....47

OXYGEN DEMAND, CHEMICAL MR .. 50

OXYGEN DEMAND, CHEMICAL HR ... 53

PHOSPHORUS, REACTIVE .............. 56

PHOSPHORUS, ACID HYDROLYZABLE ...

PHOSPHORUS, TOTAL ................... 63

PHOSPHORUS, REACTIVE HR ......... 67

PHOSPHORUS, TOTAL HR .............. 70

INTERFACE WITH PC ..................... 74

STANDARD METHODS ................... 76

BATTERY REPLACEMENT ................76

ACCESSORIES ............................... 77

CE DECLARATION OF CONFORMITY .... 78

WARRANTY ...................................79

HANNA LITERATURE ...................... 79

All rights are reserved. Reproduction in whole or in part is prohibited without

the written consent of the copyright owner, Hanna Instruments Inc.,

Woonsocket, Rhode Island, 02895 , USA.

WARRANTY

All Hanna Instruments meters are warranted for two years against

defects in workmanship and materials when used for its intended

purpose and maintained according to the instructions.

This warranty is limited to repair or replacement free of charge.

Damages due to accident, misuse, tampering or lack of prescribed

maintenance are not covered.

If service is required, contact your dealer. If under warranty, report the

model number, date of purchase, serial number and the nature of the

failure. If the repair is not covered by the warranty, you will be notified

of the charges incurred.

If the instrument is to be returned to Hanna Instruments, first obtain a

Returned Goods Authorization Number from the Customer Service

Department and then send it with shipment costs prepaid. When

shipping any instrument, make sure it is properly packaged for complete

protection.

To validate your warranty, fill out and return the enclosed warranty

card within 14 days from the date of purchase.

Hanna Instruments reserves the right to modify the design, construction

and appearance of its products without advance notice.

HANNA LITERATURE

Hanna publishes a wide range of catalogs and handbooks for an

equally wide range of applications. The reference literature currently

covers areas such as:

• Water Treatment

• Process

• Swimming Pools

• Agriculture

• Food

• Laboratory

and many others. New reference material is constantly being added to

the library.

For these and other catalogs, handbooks and leaflets contact your

dealer or the Hanna Customer Service Center nearest to you. To find

the Hanna Office in your vicinity, check our home page at

www.hannainst.com.

PRELIMINARY EXAMINATION

Please examine this product carefully. Make sure that the instrument

is not damaged. If any damage occured during shipment, please

notify your Dealer.

Each Meter is supplied complete with:

•Five Sample Vials

•Two 9 V Batteries

•12 VDC Transformer (HI 710005 or HI 710006)

•Instruction Manual

Note:Save all packing material until you are sure that the instrument

works correctly. Any defective item must be returned in its

original packing with the supplied accessories.

°C:degree Celsius

COD:Chemical Oxygen Demand

EPA:US Environmental Protection Agency

°F:degree Fahrenheit

g/L:grams per liter. g/L is equivalent to ppt (part per thoushand)

HR:high range

LR:low range

mg/L:milligrams per liter. mg/L is equivalent to ppm (part per million)

mL:milliliter

MR:medium range

µg/L:micrograms per liter. µg/L is equivalent to ppb (part per billion)

ABBREVIATIONS

Recommendations for Users

Before using these products, make sure that they are entirely suitable for your specific application

and for the environment in which they are used.

Operation of these instruments may cause unacceptable interferences to other electronic equip-

ments, this requiring the operator to take all necessary steps to correct interferences.

Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC

performance.

To avoid damages or burns, do not put the instrument in microwave ovens. For yours and the

instrument safety do not use or store the instrument in hazardous environments.

CE DECLARATION OF CONFORMITY

GENERAL DESCRIPTION

C 99 & C 200 Series is a line of 17 different bench, microprocessor-

based photometers to measure more than 50 different parameters in

water and wastewater. These multipurpose meters are manufactured

to measure the most important parameters of the application they

have been especially designed for:

C 99 Laboratories, with COD C 200 Laboratories

C 203 Aquaculture C 205 Boilers & Cooling Towers

C 206 Environmental Testing C 207 Industrial Wastewater

C 208 Water Conditioning C 209 Education

C 210 Pulp & Paper Mills C 211 Chemical Manufacturers

C 212 Power Plant Utilities C 213 Municipal Wastewater

C 214 Wastewater Treatment Applic. C 215 Nutrient Analyses

C 216 Pool & Spa Applic. C 218 Environmental Applic.

C 226 Pool & Spa Applic.

The reagents are in liquid or powder form and are supplied in bottles

and ready-to-use vials or in packets. The amount of reagent is precisely

dosed to ensure the maximum repeatability.

Display codes aid the user in routine operations.

The meters have an auto-shut off feature that will turn off the instrument

after 10 minutes of non-use.

The C 99 & C 200 Series can be connected to a personal computer via

the HI 920010 three wire RS 232 cable. The HI 92000 Hanna

Windows

Compatible Software aids the user to manage all test data.

REAGENT SETS

HI 93701-01 100 free chlorine tests

HI 93701-03 300 free chlorine tests

HI 93711-01 100 total chlorine tests

HI 93711-03 300 total chlorine tests

HI 93754A-25 25 COD LR tests

HI 93754B-25 25 COD MR tests

HI 93754C-25 25 COD HR tests

HI 93758A-50 50 reactive phosphorus tests

HI 93758B-50 50 acid hydrolyzable phosphorus tests

HI 93758C-50 50 total phosphorus tests

HI 93763A-50 50 reactive phosphorus HR tests

HI 93763B-50 50 total phosphorus HR tests

HI 93764A-25 25 ammonia LR tests

HI 93764B-25 25 ammonia HR tests

HI 93766-50 50 nitrate tests

HI 93767A-50 50 total nitrogen LR tests

HI 93767B-50 50 total nitrogen HR tests

OTHER ACCESSORIES

C115-00300 5 mL graduated syringe

C9800-01 Hanna Reactor (115 VAC)

C9800-02 Hanna Reactor (230 VAC)

HI 710005 110VAC to 12VDC voltage adapter

HI 710006 220VAC to 12VDC voltage adapter

HI 721310 9V battery (10 pcs)

HI 731311 glass vials with cap (5 pcs)

HI 731318 Tissue for wiping cuvets (4 pcs)

HI 731340 200 µL automatic pipette

HI 731341 1000 µL automatic pipette

HI 731342 2000 µL automatic pipette

HI 731350 tips for 200 µL automatic pipette (25 pcs)

HI 731351 tips for 1000 µL automatic pipette (25 pcs)

HI 731352 tips for 2000 µL automatic pipette (4 pcs)

HI 740142 1 mL graduated syringe

HI 740143 1 mL graduated syringe (6 pcs)

HI 740144 Pipette tip (6 pcs)

HI 740157 Plastic refilling pipette (20 pcs)

HI 740216 Test tube cooling rack (25 holes)

HI 740217 Laboratory bench safety shield

HI 92000 Windows

Compatible Software

HI 920010 PC connection cable

HI 93703-50 Cuvets cleaning solution (230 mL)

ACCESSORIES

SPECIFICATIONS

Light Life Life of the instrument

Light Detector Silicon Photocell

Environment 0 to 50°C (32 to 122°F);

max 90% RH non-condensing

Power Supply 2 x 9 V batteries / 12 to 20 VDC through

voltage adapter

Auto-Shut off After 10' of non-use

Dimensions 230 x 165 x 70 mm (9.0 x 6.5 x 2.8")

Weight 640 g (22.6 oz.)

For specifications related to each single parameter (e.g. range, precision,

etc.), refer to the related measurement section.

PRECISION AND ACCURACY

Precision is how closely repeated measurements agree with each other.

Precision is usually expressed as standard deviation (SD).

Accuracy is defined as the nearness of a test result to the true value.

Although good precision suggests good accuracy, precise results can be

inaccurate. The figure explains these definitions.

For each parameter, the precision is expressed in the related measurement

section as standard deviation at a specific concentration value of the

analite. The standard deviation is obtained with a single instrument using

a representative lot of reagents.

Description Range Method

Ammonia LR 0.00 to 3.00 mg/LNessler

Ammonia HR 0 to 100 mg/LNessler

Chlorine, Free 0.00 to 5.00 mg/LDPD

Chlorine, Total 0.00 to 5.00 mg/LDPD

Nitrate 0.0 to 30.0 mg/LChromotropic Acid

Nitrogen, Total 0.0 to 25.0 mg/LChromotropic Acid

Nitrogen, Total HR 10 to 150 mg/LChromotropic Acid

COD LR 0 to 150 mg/LDichromate, Mercuric Sulphate

COD MR 0 to 1500 mg/LDichromate, Mercuric Sulphate

COD HR 0 to 15000 mg/LDichromate, Mercuric Sulphate

Phosphorus, Reactive 0.00 to 5.00 mg/LAscorbic Acid

Phosphorus, Acid Hydrolyzable 0.00 to 5.00 mg/LAscorbic Acid

Phosphorus, Total 0.00 to 3.50 mg/LAscorbic Acid

Phosphorus, Reactive HR 0.0 to 100.0 mg/LVanadomolybdophosphoric Acid

Phosphorus, Total HR 0.0 to 100.0 mg/LVanadomolybdophosphoric Acid

STANDARD METHODS

Besides the 12 VDC supply, this meter can also be fed by two 9V

batteries.

To prolong battery life, switch your meter off after use. However, the

meter has an auto-shut off feature that will turn itself off after 10

minutes of non-use.

A blinking "LOBAT" on the display, while a

measurement is in progress, indicates low

voltage and the batteries should be replaced.

If the batteries are not replaced immediately,

in order to prevent erroneous readings due to

low voltage, "-BA-" is displayed soon

afterwards. At this point the batteries must be

changed.

Battery replacement must only take place in a non-hazardous area

using two 9V alkaline batteries.

Remove the battery cover on the back of photometer, attach two fresh

9V batteries, while paying attention to the correct polarity, and replace

the cover.

The meter will turn on automatically when a

new battery is connected. You can turn it off

by pressing ON/OFF.

BATTERY REPLACEMENT

PRINCIPLE OF OPERATION

Absorption of Light is a typical phenomenon of interaction between

electromagnetic radiation and matter. When a light beam crosses a

substance, some of the radiation may be absorbed by atoms, molecules

or crystal lattices.

If pure absorption occurs, the fraction of light absorbed depends both

on the optical path length through the matter and on the physical-

chemical characteristics of substance according to the Lambert-Beer

Law:

-log

ελc d

A ελc d

Where:

-log

=Absorbance (A)

=intensity of incident light beam

=intensity of light beam after absorption

ελ=molar extinction coefficient at wavelength λ

c=molar concentration of the substance

d=optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance

of the substance as the other factors are known.

Photometric chemical analysis is based on the possibility to develop

an absorbing compound from a specific chemical reaction between

sample and reagents.

Given that the absorption of a compound strictly depends on the

wavelength of the incident light beam, a narrow spectral bandwidth

should be selected as well as a proper central wavelength to optimize

measurements.

The optical system of Hanna's C 99 & C 200 Series multiparameter

photometers is based on special subminiature tungsten lamps and

narrow-band interference filters to guarantee both high performance

and reliable results.

Four measuring channels (at four different wavelengths) allow a wide

range of tests.

C 200 Series block diagram (optical layout)

/PUP - Program Up

/PDN - Program Down

/PTM - Turn Test Mode On

/Brx - Set the baud rate

1 - 300 2 - 600

3 - 1200 4 - 2400

/KBL - Lock Keyboard

/KBU - Unlock Keyboard

?PR# - Send Current Program Number

?BRQ - Send current baud rate

1 - 150 2 - 300

3 - 600 4 - 1200

5 - 2400

?CNQ - Send Concentration (three bytes)

conc

decimal point

unit

m - ppm b - ppb

t - ppt u - pcu

h - pH

?ERR - send error / status information

0 - No error

1 - CAP

2 - HI

3 - ZERO

4 - LO

5 - IDLE

6 - ZERO DONE

7 - TIMED READ

A microprocessor controlled special tungsten lamp emits radiation

which is first optically conditioned and beamed to the sample contained

in the vial. The optical path is fixed by the diameter of the vial. Then

the light is spectrally filtered to a narrow spectral bandwidth, to obtain

a light beam of intensity

oor

The photoelectric cell collects the radiation

that is not absorbed by

the sample and converts it into an electric current, producing a potential

in the mV range.

The microprocessor uses this potential to convert the incoming value

into the desired measuring unit and to display it on the LCD.

The measurement process is carried out in two phases: first the meter

is zeroed and then the actual measurement is performed.

The vial has a very important role because it is an optical element and

thus requires particular attention. It is important that both the

measurement and the calibration (zeroing) vials are optically identical

to provide the same measurement conditions. Most of parameters use

the same vial for both. In each case, insert the test vials always in the

same position into the holder.

It is necessary that the surface of the vial is clean and not scratched.

This is to avoid measurement interference due to unwanted reflection

and absorption of light. It is recommended not to touch the vial walls

with hands.

Furthermore, in order to maintain the same conditions during the

zeroing and the measuring phases, it is necessary to close the vial to

prevent any contamination.

Excel©Copyright of "Microsoft Co."

Lotus 1-2-3©Copyright of "Lotus Co."

TELIX®is registered Trademark of "Deltacomm"

Windows®and Windows Terminal®are registered Trademark of "Microsoft Co."

To connect your meter to the PC use the optional HI 920010 (available

from your Hanna Dealer). Make sure that your meter is switched off

and plug the connectors, one into the meter RS 232C socket, the other

into the serial port of your PC.

Note:Cables other than HI 920010 may use a different configuration,

in which case, communication between the meter and the PC

may not be possible.

SETTING THE BAUD RATE

The transmission speed (baud rate) of the meter and the external

device must be identical. The meter is factory set to 2400.

If you wish to change this value, please contact your nearest Hanna

Center.

SENDING COMMANDS FROM PC

With terminal programs such as, for example, Telix®, Windows Termi-

nal®, it is possible to remotely control your Hanna Instruments bench

meter. Use HI 920010 cable to connect the meter to the PC, start the

terminal program and set the communication options as follows: 8, N,

1, no flow control.

Command Types

To send a command to the meter the scheme is:

This line makes the computer send a Data Link Escape character (/ or

?), the command expressed as a number or a 3-character sequence

and a CR character.

Note: Windows Terminal®and all the other terminal programs that

support the ANSI escape sequence, represent the DLE character

by the string ‘^P’ and the CR character by the string ‘^M’.

Type of Commands

/OFF - Turn the meter OFF

/PDR - Press Read Direct

/PTR - Press Timer

/PZR - Press Zero

INTERFACE WITH PC

FRONT PANEL

1) Vial Holder

2) Dual Level Liquid Crystal Display (LCD)

3) Programs List

4) READ DIRECT, to perform measurement immediately

5) TIMER, to perform measurement after a preprogrammed

countdown

6) ZERO, to zero the meter prior to measurement

7) Program tand s, to select the desired parameter

8) ON/OFF, to turn the meter on and off

REAR PANEL

1) Power Supply 12 VDC 2.5 Watt

2) RS 232 Socket

3) Batteries Compartment

FUNCTIONAL DESCRIPTION

•Wait for a few seconds and the

display will show “-0.0-”. Now the

meter is zeroed and ready for

measurement.

•Remove the blank vial.

•Place the sample vial into the holder

and push it completely down.

•Press READ DIRECT and "SIP" will blink on the display.

•The instrument directly displays concentration in mg/L of phosphate

(PO4

3-) on the Liquid Crystal Display.

The method detects free (orthophosphate), condensed inorganic

forms (meta-, pyro- and other polyphosphates) and organic forms

of phosphates present in the sample.

•To convert the reading to mg/L of P2O5, multiply by a factor of

0.748.

•To convert the reading to mg/L of phosphorus (P) concentration,

multiply by a factor of 0.326.

Note: for accurate measurements

1) wash glassware only with phosphate-free detergents

2) clean all glassware with 1 : 1 hydrochloric acid solution and rinse with

deionized water.

INTERFERENCES

Arsenate: positive error

pH: the sample should have a neutral pH

Temperature: the method is temperature sensitive.

It is recommended to add the Molybdovanadate Reagent

and to run measurements at T = 20 to 25 °C:

T < 20 °C causes a negative error

T > 25 °C causes a positive error

Turbidity: turbidity and suspended matter in large amounts may

cause interference because the strongly acidic reaction

conditions may dissolve suspended matter or cause

desorption of phosphates from particles. Before

measurement, turbidity or suspended matter should

be removed by treatment with active carbon and by

prior filtration.

Phosphorus, Total HR

GUIDE TO DISPLAY CODES

Note: The secondary LCD below shows a generic "P– –", whereas the

meter will indicate the exact program number (e.g. "P1" for

Ammonia LR).

Sampling in progress. This flashing prompt

appears each time the meter is performing

a measurement.

The microprocessor is adjusting the light

level, indicated by a scrolling "SIP".

This indicates that the meter is in a zeroed

state and measurement can be performed.

The blinking "LOBAT" indicates that the

battery voltage is getting low and the

batteries need to be replaced.

The instrument is performing an internal

check-up.

This indicates that the meter is in a ready

state and zeroing can be performed.

This indicates that the batteries are dead

and must be replaced.

Light over range. The vial is not inserted

correctly and an eccess ambient light is

reaching the detector. If the vial is properly

inserted, then contact your dealer or the

nearest Hanna Customer Service Center.

•At the end of the digestion place the

vials carefully in the test tube rack and

allow to cool to room temperature.

Warning: as the vials are still hot,

be careful in handling them.

•Select the program number corresponding to Total Phosphorus High

Range on the secondary LCD by pressing PROGRAM tand s.

•Remove the cap from the vials and add exactly 2.0 mL of Sodium

Hydroxide (NaOH) Solution 1,54 N to each vial, while keeping

the vials at a 45-degree angle. Replace the cap tightly and mix

by inverting the vials a couple of times.

•Remove the cap from the vials and add exactly 0.5 mL of HI

93763B-0 Molybdovanadate Reagent to each vial, while keeping

the vial at a 45-degree angle. Replace the cap tightly and mix by

inverting the vials a couple of times.

•Place the blank vial into the holder

and push it completely down.

•Press TIMER and the display will show the countdown prior to the

measurement. Alternatively, wait for 7 minutes and press ZERO. In

both cases "SIP" will blink during measurement.

Phosphorus, Total HR

The sample and zero vial are inverted.

This indicates that the meter has lost its

configuration. Contact your dealer or the

nearest Hanna Customer Service Center.

ERROR MESSAGES

a) on zero reading:

Theinstrumentcannot adjustthelightlevel.

Please check that the vial does not contain

any debris.

There is not enough light to perform a

measurement.Pleasecheck thepreparation

of the zero vial.

This indicates that the zeroing procedure

failed due to a low signal-to-noise ratio. In

this case press ZERO again.

There is too much light to perform a

measurement.Pleasecheck thepreparation

of the zero vial.

The lamp is not working properly. Contact

your dealer or the nearest Hanna Customer

Service Center.

The lamp is not working properly. Contact

your dealer or the nearest Hanna Customer

Service Center.

b) on sample reading:

There is too much light for the sample

measurement. Please check if the right

sample vial is inserted.

Reagent Blank Correction: This method needs a reagent blank

correction. A single blank vial may be used more than once; the blank

vial is stable up to one day (room temperature). For most accurate

measurement, run a blank for each set of measurements and always use

the same lot of reagents for blank and samples.

•Preheat the Hanna Reactor C 9800 to 150 °C (302°F). For correct

use of the reactor follow Reactor Instruction Manual.

Use of the optional HI 740217 safety shield is strongly recommended.

Do not use an oven or microwave because leaking samples can

generate a corrosive and possibly explosive atmosphere.

•Remove the cap from two Reagent Vials.

•Add exactly 5.0 mL of sample to one vial (sample vial), and 5.0

mL of deionized water to the other vial (blank vial), while keeping

the vials at a 45-degree angle.

•Add the content of one packet of Potassium Persulfate for Phosphorus

analysis to each vial. Replace the cap tightly and shake gently the

vials until all the powder is completely dissolved.

•Insert the vials into the reactor and

heat them for 30 minutes at 150°C.

Phosphorus, Total HR

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