Fisher Scientific AccuSpin 3 Manual de usuario

accuSpin™ 3 / 3R

OPERATING

INSTRUCTIONS

How to use this manual

Use this manual to get acquainted with your centri-

fuge and its accessories.

The manual helps you to avoid inappropriate han-

dling. Make sure to keep it always close to the centri-

fuge.

A manual that is not kept handy cannot provide

protection against improper handling and thus

against damage to persons and objects.

The manual comprises chapters on

• Safety regulations

• Instrument description

• Rotor program and accessories

• Transportation and hook-up

• Use of the centrifuge

• Maintenance and care

• Troubleshooting

• Technical data

• Index

Please fold out

Overleaf you will find a graphic

representation of the control panel

with a survey of the most important

functions

speed/RCF

Quick run

run time

open lid

stop

start

The mains switch is located on the right-hand

side panel.

Pressed up = ON I

Pressed down = OFF 0

(only for instruments with refrigeration unit)

temperature

program selection

Bucket selection

rpm/RCF

switch key

display "set" keys

"set" keys

pre-temperature-regulation

Before switching on the centrifuge

please read this manual

deceleration

profiles

acceleration

profiles

Control panel of the accuSpin™ 3 / 3R

Display panels

Program selection

Key 1 - 4 : store or recall programs.

Acceleration profiles (1= slowest ...... 9= fastest)

permanent

display: acceleration profile last set 1- 9

Deceleration profiles (1= slowest ...... 9= fastest)

permanent

display: deceleration profile last set 1- 9

Speed / RCF

run: current value of speed or RCF after activation of

switch key

end: "End"

lid open: "OPEN"

"Lift Lid" (if lid is not automatically lifted off)

(before start)

lid closed: "0" with flashing point

(rotor not yet identified)

error code: will flash in display

Run time

time selection: - remaining run time to 0

continuous - run time passed (HLD)

operation (hld) (in hours, minutes)

"Quick Run": - run time passed (as long as button is held;

in minutes and seconds)

Temperature*

run: current sample temperature in °C

(in temperature equilibrium)

* only for instruments with refrigeration unit

Keys

start : normal start of the centrifuge

stop : manual stop of a run

open lid: open lid (possible only with the instrument

switched on)

Quick run: short-term operation of the centrifuge as long as

key remains pressed

rpm/RCF

switch: switching between rpm and RCF display

Bucket set.: Setting of the bucket number

Pretemp : Pre-temp-function*

"set" keys: stepwise increase/decrease of setpoint values

Short pressing of any of the "set" keys: switch from current to preset

value, signalled by flashing display.

Error codes (troubleshooting see chapter "Troubleshooting"):

E-19: Unpermissible rotor

E-20: Rotor not identified

E-29: Motor or rotor blocked

E-31: Overtemperature in motor

E-32: Overtemperature in electronic

E-33: Excessive pressure in the refrigeration unit

E-34: Overvoltage

E-35: Overcurrent

Lift Lid : Smoothly lift the lid

rotor: set speed higher than permissible speed of

the rotor

bAL : unbalance

Contents

For your safety............................................ 3

Proper use................................................................ 3

Improper use ............................................................ 3

Centrifuging hazardous materials ............................ 3

Handling the centrifuge ............................................ 4

Conformity to current standards............................... 5

Safety instructions in this manual ............................ 5

The accuSpin™ 3 / 3R ................................ 7

Description ............................................................... 7

Safety systems......................................................... 8

Parts supplied .......................................................... 8

Function and features .............................................. 9

Before use................................................. 13

Centrifuge transport and installation ...................... 13

Proper location ....................................................... 13

Main connection ..................................................... 14

Rotors and accessories ........................... 15

Rotors for the accuSpin™ 3................................... 17

Rotors for the accuSpin™ 3R................................. 18

Handling the rotor................................................... 23

Aerosol-tight operation........................................... 25

Checking of aerosol-tight bio-containment......... 27

Operation .................................................. 29

Switching on the centrifuge .................................... 29

Actuating the lid...................................................... 29

Opening the lid ................................................... 29

Closing the lid ..................................................... 29

Installing the rotor................................................... 30

Loading the rotor .................................................... 31

Maximum loading ............................................... 31

Filling the centrifuge tubes ................................. 31

Maximum permissible load difference................ 32

Inserting the centrifuge tubes ............................. 32

Entering parameters............................................... 35

Deceleration curves ............................................... 35

Switching from speed to RCF display .................... 35

Bucket selection for swinging bucket rotors........... 35

Selecting speed...................................................... 36

Entering the RCF value.......................................... 36

More about the RCF value ................................. 37

Selecting run time .................................................. 37

Run time selection.............................................. 37

Continuous operation ......................................... 38

Extended time mode........................................... 38

Selecting the temperature ...................................... 39

Pretemp function .................................................... 39

Starting the centrifuge ............................................ 40

Contents

Imbalance display................................................... 40

Changing the settings during the run ..................... 40

Stopping the centrifuge .......................................... 41

Stopping with preset run time............................. 41

Stopping with continuous operation ................... 41

Temperature control during standby ...................... 41

Working with programs........................................... 42

Program display.................................................. 42

Entering/changing a program ............................. 42

Centrifuging with a program ............................... 42

”Quick Run” ............................................................ 43

Removing the rotor................................................. 43

Audible alarm ......................................................... 44

Turning the centrifuge off ....................................... 44

Maintenance and care .............................. 45

Maintenance to be performed by the customer ..... 45

Cleaning.............................................................. 45

Disinfection ......................................................... 46

Decontamination................................................. 48

Autoclaving ......................................................... 48

Troubleshooting ....................................... 49

Emergency lid release............................................ 49

Error troubleshooting.............................................. 51

Contacting Fisher ScientificService..................... 60

Technical Data .......................................... 61

Electrical connections / fuses................................. 63

Appendix ................................................... 65

Acceleration and deceleration profiles ................... 65

Speed/RCF diagrams............................................. 70

Index .......................................................... 73

For your safety

Fisher Scientificcentrifuges are manufactured accord-

ing to current technical standards and regulations.

Nonetheless, centrifuges may pose danger to individu-

als and surrounding if

• they are not used as designed

• they are operated by untrained personnel

• their design is improperly changed

• the safety instructions are not followed

Therefore, personnel involved with operation and

maintenance of the centrifuge must read and follow

the safety instructions.

In addition, the pertinent regulations for prevention of

accidents must be strictly followed

This manual is an integral part of the centri-

fuge assembly and must be kept close at

hand at all times.

When damages at the power cord or at

casing were noticed the centrifuge has

to be set out of operation!

Proper use

The centrifuge is designed to separate liquid-

suspended materials having different densities and

particle size, respectively (maximum sample density is

1.2 g/cm³ {ml} at maximum speed).

Improper use

During a run, a safety zone of 30 cm around the centri-

fuge must be maintained where neither persons nor

hazardous materials may be present.

The centrifuge may cause harm to user or other per-

sons or may damage goods if safety measures are not

followed:

Centrifuging hazardous materials

• The centrifuge is neither made inert, nor is it explo-

sion-proof. Therefore never use the centrifuge in an

explosion-prone environment.

• Do not centrifuge explosive or flammable sub-

stances must not be centrifuged. The same holds

for substances prone to react violently with each

other.

For your safety

• Do not centrifuge toxic or radioactive substances or

pathogenic microorganisms without suitable safety

systems.

If microbiological samples of risk group II (according

to "Laboratory Bio-safety Manual" of WHO) are be-

ing centrifuged, aerosol-tight bio-seals have to be

used.

For materials with a higher risk group, more than

one precaution is required.

• Should toxins or pathogenic substances enter the

centrifuge or its parts, you must perform appropriate

procedures for disinfection (see "Maintenance and

care – Disinfection").

• Strongly corrosive substances that may cause

damage to materials and reduce the mechanical

strength of the rotor, may be centrifuged only inside

protective tubes.

Handling the centrifuge

• Use only original accessories for the centrifuge. The

only exception are common glass or plastic centri-

fuge tubes, if they are approved for the rotor speed

and RCF values.

• Never use the centrifuge unless the rotor is properly

installed.

• You may use the centrifuge only with a properly

loaded rotor. You must not overload the rotor.

• Strictly follow the rules and regulations for cleaning

and disinfection

• If the rotor or the rotor lid shows signs of corrosion

or wear, you must stop using it.

• Never open the lid manually if the rotor still turns.

• You may use the emergency lid release only in

case of emergency, e.g. during an interruption of

power supply

(see chapter "Troubleshooting").

• Never use the centrifuge with the lid open.

• Never use the centrifuge if the front panel has been

partially or totally removed.

• Changes in mechanical or electrical components of

the centrifuge may be carried out only by individuals

authorized by Fisher Scientific.

For your safety

Conformity to current standards

Fisher Scientificcentrifuges are manufactured and

tested according to the following standards and regula-

tions:

- for all voltages

• IEC 61010

- for 120 V only

•

- for 230 V only

•

Details of the test standards take you please from the

technical data.

Safety instructions in this manual

This symbol denotes potential hazards to

persons.

This symbol denotes potential damage to

the centrifuge or parts in its immediate

surroundings.

General hints are marked with this

symbol.

For your safety

Notes

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