ETT Gustometer 2 Manual de usuario

ETT Gustometer 2

Gustatory Stimulation

Manual and Tutorial

Revision 2021.1

 
2 
© Emerging Tech Trans, LLC 
Table of Contents 
Table of Contents........................................................................................................................................ 2 
Setup........................................................................................................................................................... 3 
Safety Information.................................................................................................................................. 3 
Warnings................................................................................................................................................. 3 
Contact to ETT......................................................................................................................................... 3 
Connecting the Device............................................................................................................................ 3 
Before the first power up.................................................................................................................... 3 
System components ............................................................................................................................... 3 
System Overview .................................................................................................................................... 6 
Tastant setup ...................................................................................................................................... 7 
Tastant applicator / Mouth piece setup.................................................................................................. 7 
Applicator tubing ................................................................................................................................ 7 
Coil mounting (fMRI package)............................................................................................................. 7 
Fitting a subject................................................................................................................................... 8 
The first experiment ............................................................................................................................... 8 
Typical tastants ................................................................................................................................... 9 
Touch-screen operating system.......................................................................................................... 9 
Resources.................................................................................................................................................. 13 
Useful information................................................................................................................................ 13 
Storage.............................................................................................................................................. 13 
Cleaning procedures ......................................................................................................................... 13 
Trouble shooting............................................................................................................................... 15 
Data management ............................................................................................................................ 15 
 
 

Setup

Safety Information

Do not open the device without explicit instructions from ETT customer support. Before opening the

device disconnect the AC power supply cable!

Warnings

1. This device is for research use only!

2. Item 1 on the system components list is not MRI compatible.

3. Only the tubing and the applicator/mouthpiece are MRI compatible and thus allowed into the

magnet room.

4. Feed all tubing through an appropriately sized wave guide to avoid damage to the connectors.

Contact to ETT

Address

Emerging Tech Trans, LLC

2151 Harvey Mitchell Parkway S

STE 209

College Station, TX 77840, USA

info@emergingtechtrans.com

Connecting the Device

Before the first power up

After receiving and unboxing the device inspect the ETT Gustometer 2 for visible damage to both the

package and the actual device. Do not power on the device if there are visible damages or audibly

dislodged parts within the case. Consult with ETT support prior to continuing. If the device passes the

inspection, use the provided power cord, and plug in the device. The compatible AC line voltage range is

from 100 V to 240 V.

Figure 1 - AC power supply port compatible to most countries’ AC line voltage.

System components

Congratulations to your new ETT Gustatometer 2!

System components

1. The ETT Gustometer 2

Figure 2 - Front panel with touch-screen and interface connectors

Figure 3 - Back panel with pumps and inlet and outlet tubing, and air pump output

2. Set of food safe bottles for the stimulants (flavored fluids), up to 8 at a time can be stored on

the top of the device

Figure 4 - Provided glass jar with Teflon cap for stimulant storage

3. Customized mouthpiece/applicator (attaches to the RF coil or a provided table clamp; MR and

EEG compatible)

Figure 5 - Applicator can clip to RF coil structure and delivers tastant at correct angle without drops getting stuck

4. PTFE tubing set (8 m, 8 channel) between applicator and device (fed through waveguide on MR

penetration panel to connect MR compatible components with the actual device sitting in the

control or operator room)

Figure 6 - The long PTFE tubing set with blue protective cover connects the subject mouthpiece with the device

5. PTFE Inlet tubing set between tastant bottles and device

6. Swallow sensor belt with digital input (optional)

7. Coax connector (connects the device trigger in port to the BNC pin of the MR RF trigger

converter; not needed on non-Siemens systems)

8. MRI RF Trigger converter (converts an optical trigger from the RF cabinet of the MRI system to a

TTL electrical signal that triggers the ETT Gustometer or ETT Olfactometer system; not needed

on non-Siemens systems)

System Overview

Figure 7 - ETT Gustometer 2 system overview with left - device (non-MR compatible) and right - applicator (mouthpiece) on

subject (MR compatible))

The general system setup is shown in Figure 7. It is advisable to first setup the components unrelated to

the subject and test everything is connected before setting up a research subject on the patient table.

Warning - the main device is not MR compatible.

Only the following components are MR compatible:

•Mouthpiece / tastant applicator

•RF coil mount

•Swallow / respiratory sensor bladders

•Long distance PTFE connection tubing

All other components cannot be brought inside the magnetic field.

Start by setting up the ETT Gustometer 2 outside the experimentation room. Even for EEG experiments

it might be advisable to maintain a wall separation between subject and device. Each pump has a slightly

different pitch, which might inform the subject which is the currently active stimulus.

Connect the power supply cable and power the Gustometer up by pressing the power button on the

front panel. Connect a USB flash drive to the DATA port. Optionally connect the various triggers and

sensor that may be provided with your system. The ETT Gustometer 2 can easily be triggered with a TTL

pulse from and other device via Trigger in BNC port. The sent pulse should be at least 1 ms long. To

initialize the trigger handler, load a paradigm and press Trig Start to wait for a trigger. As soon as the

trigger is visible to the device the paradigm starts.

Consult with support@emergingtechtrans.com for more detailed instructions on subcomponents.

Tastant setup

There are up to eight slots for the glass tastant containers with the pre-drilled Teflon caps on top of the

Gustometer (Figure 8). As signified by the blue arrow the inlet tubing connects through the cap of the

bottle to the top Luer inlet port on the Gustometer. From there the fluid goes through the peristaltic

pump. On the back side, each channel has a Luer outlet connector. The eight channel PTFE main tubing

set connects from there to the mouthpiece.

Figure 8 - Up to eight glass containers can be stored on top of the Gustometer –one for each channel.

Tastant applicator / Mouthpiece setup

The mouthpiece setup is the most important part of the experimental setup. To assure data quality

during experiments, the mouthpiece must be parallel to the direction of gravity. If the standard

mouthpiece as provided with the Gustometer does not suit the needs experimental requirements,

contact ETT support for a custom design.

Contact ETT for service or replacement of mouth pieces.

Applicator tubing

Proper routing of the PTFE tubing is important, to allow drop formation especially for small delivery

amounts. If mouthpieces are reused, check correct fit after each cleaning cycle and prior each

experiment:

-Tubing must extend the panel inside the mouthpiece by about 1 mm (bonded in place after

2016)

-Tubing has no bends or kinks

-Tubing is free of leaks

-Tubing Is straight and the ends are cut at a straight angle

Coil mounting (fMRI package)

The ETT Gustometer 2 comes with a standard mounting system for a Siemens 12 and 20 channel clinical

head coil mounts. For other systems we can create a custom fit solution upon request.

The clamp attaches to the upper side of one or two head coil beams. Only friction between the coil and

the clamp hold the piece in place. Therefore, it slides easily along the direction of the main magnetic

field, to fit many head sizes. It allows also to be adjusted with an angle of up to 5 degrees to fit different

face geometries.

Figure 9 –Applicator setup on a Siemens 12CH head coil

The actual mouthpiece is connected to the clamp though a two-rack track, which allows for height

adjustments relative to the patient’s mouth. A retaining pin holds the two at a fixed elevation during the

experiment.

In case of an emergency the clamp allows to easily slide the whole setup upwards, with just the force of

the subjects’ tongue.

Fitting a subject

During the subject setup it is advised to fit the clamp to the upper part of the head coil first. While the

subject gets situated in the coil, slide the mouthpiece into the clamp into the most upward position

without securing it yet with the pin. Now slowly join the coil with the subjects’ mouth. Confirm with the

subject that they get each tastant during the standard test run through all channels.

The first experiment

Before the first experiment can be designed a few thoughts on your options with the ETT Gustometer.

Stimuli will be delivered in form of drops of liquid through a system of Teflon tubing through an

applicator into the subjects’ mouth. These drops of substances that will stimulate the gustatory

sensation (tastant) are commonly a chemical of choice dissolved or suspended in distilled water (dH2O).

Alternatively, a specific neutral rinse, which usually mainly consists of distilled water can be chosen as

the carrier fluid. If the particle size is small enough even emulsions with high liquid portion are

deliverable.

The ETT Gustatometer offers the option to deliver with 7 independent taste channels and one additional

rinse channel. The experiment is be split into recurring cycles. During these cycles one will have a

determined amount of tastant from one of the taste channels and one or more rinse cycles with a

defined bolus.

Typical tastants

Typically, researchers will deliver particles that are easily dissolved in water and where the formulation

is reproducible the same for every experiment. Generally, know there are five categories of taste

receptors that can detect:

-Sweetness

-Saltiness

-Bitterness

-Sourness

-Savoriness

To get a start, different concentrations of salt (NaCl), sugar/sucrose (C12H22O11), caffeine (C8H10N4O2) and

citric acid (C6H8O7) are easy to create with distilled water.

It is important to assure a proper drop formation at the mouthpiece. If the number of drops is set too

low for a tastant with a high viscosity, the drop may just grow bigger at the tip of the mouthpiece but

not actually fall onto the tongue in time. This aspect should be tested thoroughly and experimentally

adjusted to the specific requirements of the study.

Touch-screen operating system

Main Screen

Once the device is started it will present a main screen. From this screen all major features can be

controlled. The top horizontal bar contains three main features and the Help button. The vertical button

on the right side (Figure 10s). The File Manager is used to create a subject and run specific folder (see

section Creating data files on page 15) on the attached USB flash drive (connected to DATA port on front

panel).

Figure 10 - The main screen contains general settings in the top bar; paradigm test and start on the right side; and current

paradigm state information in the main area

The Program button will lead to a selection of saved paradigms and allows to create new paradigms. The

currently selected paradigm ID is presented on the Main Screen on the left section of the dark blue

block labeled Paradigm. N. It is followed by the current subject ID as it was created in the File Manager.

The button PC/Manual/Prime opens the Manual Mode Screen. Here the user can open and close

individual channels as well as prime the whole system automatically. The priming feature is also useful

during the cleaning process of the Gustometer tubing (see section Cleaning procedures on page 13).

The button PARA CHECK simulates the execution of the paradigm to confirm on screen if timings are

accurate. TRIG START enables the Input Trigger coaxial port on the front panel. If active the paradigm

execution will be delayed until the rising edge of a 5V TTL pulse is measured on this port. The PARA

START button will start the execution of a program created in the Program screen.

The Main screen also allows to change the current pump speed, displayed as a percentage of the

maximum speed. The large light blue area of the screen on the bottom half of the Main screen displays

the current state of the device within a program. The bottom row displays the current multiplier for

channels 1-7 as well as Rinse for the current paradigm. The large white bar below shows the progress

during paradigm execution. Above the user can quickly see the current paradigm configuration. Starting

on the left a general delay time before the paradigm loops start is displayed. Next to it is the number

and duration of optional initial rinses.

The section to the right visualizes the actual paradigm loop with the currently used channel ID. Stim

displays the volume (based on multiplier set below) delivered during this loop. Res next to it is the

allowed response time after the bolus is delivered.

Each stimulus application and response period is followed by a settable amount of rinse cycles. To clean

the pallet. Rinse is the number of times the system rinses with Duration being the time after each rinse.

That is followed by a Rest period until the next paradigm block in the program is administered.

Before a further explanation on the actual paradigm/Program designer let’s have a look at the Manual

Mode screen.

Manual mode

The Manual screen can be used for both priming as explained below as well as debugging and testing

each channel. It also allows to switch to PC control via the ETT Direct Control.

Figure 11 - Activate the Manual button the manually start and stop the peristaltic pumps at the currently set pump speed for

each channel

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