Dionex AS-HV Manual de usuario

AS-HV Autosampler

Operator's Manual

Document No. 065125

Revision 01

October 2006

Printed in the United States of America.

This publication is protected by federal copyright law. No part of this publication

may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or

transmitted into any human or computer language, in any form or by any means,

electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties

without the express written permission of Dionex Corporation, 1228 Titan Way,

Sunnyvale, California 94088-3603 U.S.A.

DISCLAIMER OF WARRANTY AND LIMITED WARRANTY

THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANTY OF

ANY KIND. DIONEX CORPORATION DOES NOT WARRANT,

GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED

REPRESENTATIONS REGARDING THE USE, OR THE RESULTS OF THE

USE, OF THIS PUBLICATION IN TERMS OF CORRECTNESS, ACCURACY,

RELIABILITY, CURRENTNESS, OR OTHERWISE. FURTHER, DIONEX

CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION

AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT

HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO

NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR

CHANGES.

TRADEMARKS

Chromeleon® is a registered trademark of Dionex Corporation. Self-Regenerating

Suppressor™ and SRS™ are trademarks of Dionex Corporation.

MASTERFLEX® C/L® is a registered trademark of Barnant Company.

Microsoft® Windows® XP and Windows® 2000 are registered trademarks of

Microsoft Corporation.

Nalgene® is a registered trademark of Nalge Nunc International.

PEEK™ is a trademark of Victrex PLC.

Plexiglas® is a registered trademark of Rohm & Haas Company.

Santoprene® is a registered trademark of Advanced Elastomer Systems, L.P.

Tefzel® is a registered trademark of E.I. duPont de Nemours & Company.

PRINTING HISTORY

Revision 01, October 2006

Doc. 065125-01 10/06 i

Contents

1 • Introduction

1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

1.2.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

1.3 Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

2•Description

2.1 AS-HV Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2.2 AS-HV Side Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2.3 Sample Loading Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.4 Sample Loading Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.4.1 Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

2.4.2 AXP Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2.4.3 External Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.4.4 Internal Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.4.5 Relay-Controlled Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.5 Chromeleon Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

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3 • Operation and Maintenance

3.1 Safety Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21

3.2 Getting Ready to Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22

3.2.1 Filling Sample Containers and Loading the Racks . . . . . . . . . .22

3.2.2 Turning On the Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

3.2.3 Selecting the Rinse Pump Speed . . . . . . . . . . . . . . . . . . . . . . . .24

3.2.4 Creating a Chromeleon Program . . . . . . . . . . . . . . . . . . . . . . . .25

3.3 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

3.3.1 Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37

3.3.2 Weekly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

3.3.3 Monthly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

3.3.4 Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38

4 • Troubleshooting

4.1 Liquid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

4.1.1 Leaking Fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

4.1.2 Leaking Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

4.1.3 Leaking Waste Line (Fixed Rinse Reservoir) . . . . . . . . . . . . . .40

4.2 Sampling Arm Jams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

4.3 Initialization Sequence Fails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42

4.4 Power LED Fails to Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

4.5 CPU Activity LED Fails to Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

4.6 Power Supply Fails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44

4.7 Cavitation in the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Contents

Doc. 065125-01 10/06 iii

5•Service

5.1 Cleaning the Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.2 Replacing the Sample Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.2.1 Removing the Existing Syringe . . . . . . . . . . . . . . . . . . . . . . . . 48

5.2.2 Filling the New Syringe and Removing Bubbles . . . . . . . . . . . 49

5.2.3 Installing the New Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.2.4 Initializing the New Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.3 Replacing the Sampling Needle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

5.3.1 Removing the Existing Sampling Needle . . . . . . . . . . . . . . . . 52

5.3.2 Installing the New Sampling Needle . . . . . . . . . . . . . . . . . . . . 53

5.4 Replacing the Syringe Pump Distribution Valve . . . . . . . . . . . . . . . . . 55

5.4.1 Removing the Existing Valve . . . . . . . . . . . . . . . . . . . . . . . . . . 55

5.4.2 Installing the New Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

5.5 Replacing the Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

A • Specifications

A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

A.2 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

A.3 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

A.4 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

AS-HV Operator’s Manual

iv Doc. 065125-01 10/06

B • Installation

B.1 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

B.2 Unpacking the AS-HV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

B.3 Installing the AS-HV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62

B.3.1 Installing the Spill Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62

B.3.2 Installing the Rack Location Mat. . . . . . . . . . . . . . . . . . . . . . . .63

B.3.3 Installing the Fixed Rinse Reservoir . . . . . . . . . . . . . . . . . . . . .64

B.3.4 Connecting the Rinse Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . .65

B.3.5 Installing the Standards Rack. . . . . . . . . . . . . . . . . . . . . . . . . . .66

B.3.6 Installing the Sample Rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . .67

B.3.7 Installing the Trace Analysis Cover (Optional) . . . . . . . . . . . . .67

B.3.8 Installing the Sampling Needle . . . . . . . . . . . . . . . . . . . . . . . . .68

B.3.9 Connecting the Syringe Pump (Optional) . . . . . . . . . . . . . . . . .71

B.3.10 Connecting the AXP Pump (Optional). . . . . . . . . . . . . . . . . . . .73

B.3.11 Connecting the External Peristaltic Pump (Optional) . . . . . . . .75

B.3.12 Connecting the Internal Peristaltic Pump (Optional) . . . . . . . . .77

B.3.13 Connecting a Relay-Controlled Pump (Optional) . . . . . . . . . . .77

B.3.14 Connecting the AS-HV to the Chromeleon PC . . . . . . . . . . . . .78

B.3.15 Connecting the Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . .79

B.3.16 Configuring the AS-HV in Chromeleon. . . . . . . . . . . . . . . . . . .80

B.3.17 Removing Bubbles from the Syringe. . . . . . . . . . . . . . . . . . . . .82

Contents

Doc. 065125-01 10/06 v

C • Error Messages and Codes

C.1 Audit Trail Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

C.2 AS-HV ERROR Indicator Codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

D • Reordering Information

AS-HV Operator’s Manual

vi Doc. 065125-01 10/06

Doc. 065125-01 10/06 1

1 • Introduction

1.1 Overview

The Dionex AS-HV Autosampler is a random access robotic device that can be

commanded to execute a series of liquid handling steps. The AS-HV can hold up

to 250 mL of sample and, through the use of a sample loading pump, deliver

sample to an ion chromatography (IC) system or other analyzer. Sample loading

pump options include a syringe pump, piston pump, or peristaltic pump (for

details, refer to Section 2.4).

The AS-HV is designed to accommodate a variety of accessories, including a

sample rack, a standards rack, and a rinse reservoir. The standard configuration

for the AS-HV includes the following components:

•A 24-position sample rack that accommodates 250 mL Nunclon sample flasks

•An 11-position standards rack for loading of standards and/or samples

•A PEEK™sampling needle for aspirating sample from sample containers

•A sampling needle guide that prevents the PEEK sampling needle from

bending or flexing as it pierces a septum

•A rack location mat that houses the sample rack and ensures alignment of the

sampling needle over each vial

•A built-in rinse station (consisting of a peristaltic pump and a 150 mm

reservoir that circulates rinse fluid) that can be used to clean the sampling

needle between samples

•A spill tray for secondary containment of samples

•An RS-232 cable for communication with a PC (personal computer)

The AS-HV is usually controlled remotely from a PC running Microsoft®

Windows® XP or Windows® 2000 operating system and Dionex Chromeleon®

Chromatography Management System software. The AS-HV communicates with

the PC via the RS-232 interface. Limited remote control is also available, using

relay signals.

AS-HV Operator’s Manual

2Doc. 065125-01 10/06

1.2 About This Manual

Chapter 1

Introduction An overview of the AS-HV; includes a brief description of

the standard AS-HV configuration and the software

required for operation. An explanation of the conventions

used in this manual (including safety-related information).

Chapter 2

Description Descriptions of physical aspects of the AS-HV, including

mechanical components and the LEDs, controls and

connectors on the side panel; an explanation of sample

loading modes; and an introduction to Chromeleon

software.

Chapter 3

Operation and

Maintenance

Procedures to complete before beginning operation,

instructions for creating Chromeleon programs for AS-HV

control, and routine preventive maintenance requirements.

Chapter 4

Troubleshooting Minor problems that may occur during operation, with

step-by-step procedures for how to isolate and eliminate

the cause of each problem.

Chapter 5

Service Step-by-step instructions for routine service and parts

replacement procedures for the AS-HV.

Appendix A

Specifications Specifications and installation site requirements for the

AS-HV.

Appendix B

Installation Installation instructions for the AS-HV.

Appendix C

Error Messages

and Codes

Chromeleon Audit Trail error messages and AS-HV

ERROR indicator codes, along with an explanation of the

possible cause of each problem and the corrective action to

take.

Appendix D

Reordering

Information

Spare parts for the AS-HV.

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