Dagan 2400A Manual de usuario
2400A
Extracellular Preamplifier
Operating Manual
Warning: This instrument is not designed or intended for use in
human applications or human experimentation
深圳市净康科技有限公司
地址：深圳市龙岗区南湾街道吉厦社区沙平北路 111 号 6008
王经理 15813841944（微信同号） QQ : 422612157
电话：0755-28917660 邮箱：[email protected]
网址：http://www.3000buy.com
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CONTENTS
I. GENERAL INFORMATION
A.INTRODUCTION 7
B. INSTRUMENT AND ACCESSORIES 7
C. SPECIFICATIONS 8
D. OPTIONS/ Input Connectors 9
II. DIRECTIONS FOR USE
A. INSTALLATION 10
1. Line Voltages and Frequency
2. Rack Mounting
3. Bench Use
4. Cleaning
B.OPERATION
1. Electrode 11
2. Stain 11
3.CurrentPump 12
a) Current Pump Compliance 12
b) External Pump Level Reference 12
c) Auxiliary Pump Output 13
d) Z Test (Impedance Test) 13
4. Preamplifier 15
a) Use of High and Low Cutoff Filters 16
b) Monitor Amplifier 17
5. Probe 18
a) Capacitive Neutralization Control 18
b) Probe Input Parameters 19
c) Probe Input Connector 20
d)SystemGrounding 20
e) Physical Probe Placement 20
C. MODEL B (external DC/Battery operation) 22
D.SERVICING 22
E.GUARANTEE 23
DAGAN CORPORATION Minneapolis, Minnesota USA
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I GENERAL INFORMATION
A. INTRODUCTION
The DAGAN Model 2400 EXTRACELLULAR PREAMPLIFIER CURRENT
PUMP is a low noise preamplifier system for extracellular recording
with both glass and metal microelectrodes or micropipettes.
The high input impedance assures faithful reproduction from
electrodes ranging into the hundreds of megohms. In addition to
producing low noise voltage preamplification, the 2400 is unique in
that it also provides an audio amplifier for audible monitoring of the
preamplifier output and two types of current injection to the
microelectrode. One type is used for high current cell staining and
the second is a precise ideal constant current source capable of both
front panel and external analog programming control of magnitude
and polarity. It is potentially useful for cell staining, iontophoretic
drug injection and as a stimulating current (polarize or depolarize).
In addition the Model 2400Z uses the CURRENT PUMP for
microelectrode resistance measurements.
The 2400 also provides optimum bandwidth tailoring for maximum
signal to noise ratio by incorporating both active high pass and low
pass push button switch selection filters. And to reject AC line noise,
an independently, operated LINE NOTCH FILTER provides a 200 to 1
reduction in 60 Hertz interference. When powering the 2400 from
standard AC power lines, no batteries are ever needed to achieve
the low noise performance.
The 2400 is packaged within an attractive, heavy-duty cabinet for
either rack or bench mounting. When used by itself or as a
component of a larger system, this instrument will truly become an
indispensable laboratory tool.
B. INSTRUMENT & ACCESSORIES
The Model 2400A is shipped with the following standard accessories:
Rack adapter (handles/bracket assemblies).
Probe mounting rod for micromanipulator
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C. SPECIFICATIONS
PREAMPLIFIER
NEUTRALIZATION: 15 turn control located on probe. Range is 0 to 100
pF.
INPUT IMPEDANCE: Greater than 10,000 Megohms
INPUT CONNECTOR: Miniature co-axial slip-on
OUTPUT: 10 ohms impedance. 20 volt peak to peak,
BNC connector
GAIN: 50; 100; 200; 500; 1,000; 2,000; 5,000; or 10,000 ± 1%
OVERLOAD RECOVERY: Recovery is very fast, usually
one-half to a few milliseconds except for just after
STAIN. Recovery is then 20 to 40 seconds.
NOISE: Due to Amplifier and Source Resistance; (takes
into account current and voltage noise)
CALIBRATE GENERATOR: 100 uV ± 1%, Peak to Peak Square Wave. 150
Hz
LINE NOTCH FILTER: Standard 60 Hz. (optional 50 Hz.) The rejection ratio
is 200 to or, 46 dB.
HIGH FREQUENCY FILTER: Active 20 dB/decade, push button switch
selection 3 dB points are; 100, 300, 1K. 3K, 10K, 30K and 100K Hz.
LOW FREQUENCY FILTER: Active 20 dB/decade, push button switch
selection 3 dB points are 0.3, 1.0, 3.0, 10, 30, 100, and 300 Hz.
AUDIO MONITOR: An internal audio power amplifier monitors the
PREAMPLIFIER output. Sound level is controlled by the MONITOR control.
Output is through a phone jack located on rear panel. An 8 ohm speaker,
or external computer type powered amplifier is recommended.
CURRENT PUMP
PUMP LEVEL:
(1) EXTERNAL: An analog voltage between minus 10
to plus 10 volts into the EXT REF BNC jack generates
a current between minus 1000 nA to plus 1000 nA.
(2) INTERNAL: The INTERNAL position enables the 10
digital dial current setting. The dial is read out
in nA, and has a range of 0 to 1000 nA. Polarity is determined by
the PUMP POLARITY switch.
Z TEST: A signal 2 Hz 100mV per megohm
STAIN
A POSITIVE (for Metal electrodes) or NEGATIVE (for Glass electrodes)
voltage of 15 volts limited by 10,000 ohms is fed into the electrode.
ELECTRODE (PROBE MODE)
A four station push button switch selects the mode of operation by
connecting the probe input connector to the four functions via a reed relay
located in the probe. The four functions are:
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(1) STAIN: The high level STAIN current is fed to the microelectrode.
(2) CURRENT PUMP: The CURRENT PUMP section of the instrument is
connected through the probe to the electrode. The CURRENT PUMP
is always available through the AUX PUMP OUT BNC jack.
(3) PREAMPLIFIER: The probe and audio monitor amplifiers are connected
to the electrode.
(4) EXTERNAL: The electrode is connected to the EXTERNAL BNC sack. This
position gives direct access to the electrode for passage of any
voltage or current.
GENERAL
POWER: 115v AC, 60 Hz, 6 Watts (optional 230v AC, 50 Hz, 4 Watts)
DIMENSIONS:
MAINFRAME: 43.2 cm W (17") X 34.3 cm D (13k") X 13.3cm H (5
1/4”) (for Rack mounting, handle and brackets make width 48.3 cm
(19").
PROBE: 5.7 cm (2Y4") X 3.2 cm (PA") X 2.5 cm (1") less mounting
plate. Detachable rod is 0.63 cm dia. (1/4 ") X 7.6 cm long (3").
Probe to Mainframe cable is 7 conductor, low noise shielded, 3
meters long (10').
WEIGHT: 10Kg. (20 pounds)
D OPTIONS/ Input Connectors
2400 OPTIONAL HEADSTAGES
2410A Standard Capacitive Neutralized Single ended probe and
cable with connector.
2430A Optional Differential probe for the 2400A System
Input Adapters and Connectors
8012 Additional co-axial input connector
HB120 and HB180 Glass Microelectrode Holders for BNC option input
1mm pins for Tip Jack Option (Order# P657-5 (set of 5))
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II DIRECTIONS FOR USE
A. INSTALLATION
1. LINE VOLTAGES AND FREQUENCY
The serial number label on the instrument rear panel also contains
the line voltage and frequency. A voltage marked 115v AC indicates
the power supply is wired for voltages from 100 to 125v AC.
A 230v AC marking indicates a 200 to 250v AC range. The power
supply will operate from 50 or 60 Hz without modification. The
frequency indicated on the label identifies the frequency of the line
notch filter.
2. RACK MOUNTING
The 2400 is shipped with its rack adapters (handles and brackets)
in place. It is designed for use with a standard 19” rack.
3. BENCH USE
If desired, the rack adapters may be removed to allow for more
space on the bench.
4. CLEANING
Use a soft cloth and mild soap to maintain the dead front
appearance of the front panel.
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B. OPERATION
ELECTRODE
The ELECTRODE control switches provide a unique
combination of microelectrode terminations~ A push of
the button changes the 2400 system from (1) a high
current stain generator for marking recording sites to a,
(2) controlled positive or negative ideal current source
up to 1000nA to (3) a low noise neutralized preamplifier
to your microelectrode for any future need that you
may encounter. All this without ever touching the
probe or the microelectrode.
STAIN
Your microelectrode recording site can be marked for
easy visual identification by using either the STAIN
switch or the controlled but smaller currents from the
CURRENT PUMP. The STAIN generator provides a high
level resistive limited current with polarity selections
for use with both metal and glass electrodes.
The 2400 also provides for any future stain requirements you may
have by giving you direct access to the electrode via the EXTERNAL
BNC CONNECTOR.
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