CORNING Axygen Axypet Manual de usuario

A Corning Brand

Axygen® Axypet®

Pipettors

Single-channel, 8-channel,

and 12-channel Pipettors

Instruction Manual

Table of Contents

1. Product Description ..............................................................1

2. Setting the Volume................................................................5

3. Aspiration and Dispensing Instructions........................... 8

4. Pre-rinsing................................................................................ 9

5. Dense and Viscous Liquids.................................................10

6. Axygen® Pipet Tips...............................................................10

7. Recommendations...............................................................10

8. Recalibration .........................................................................11

9. Troubleshooting ...................................................................15

10. Cleaning and Sterilization..................................................17

11. Components..........................................................................18

12. Spare Parts .............................................................................18

13. Limited Warranty .................................................................19

Instruction Manual | 1

1. Product Description

The Axygen® Axypet® single-channel pipettor is a

volumetric instrument designed to measure and transfer

liquids precisely and safely. It can accommodate volumes

from 0.1 μL to 10,000 μL depending on the model.

Axygen Axypet 8-channel and 12-channel pipettors have

been designed for microplate filling. The pipettors enable

precise and simultaneous delivery of 8 or 12 preset volume

doses of liquid. These pipettors are produced in four volume

ranges: 0.5 to 10 μL, 5 to 50 μL, 20 to 200 μL, 50 to 300 μL.

Model Volume Range (μL) Application

2, 10

Measurement and transfer of

micro-volumes, DNA sequencing,

and enzyme assay applications.

20, 50, 100, 200,

250, 300, 1,000

Measurement and transfer of general

aqueous solution, acids, and bases.

5,000,

10,000

Measurement and transfer of large

volumes.

The single-, 8-, and 12-channel pipettors feature a digital

counter which shows the pipetting volume. The set

volume is visible in the window on the handle. Volumes

are set by either turning the pipetting pushbutton (A1) or

the adjustment knob (B) in the correct direction.

A1. Pushbutton

A2. Knob

B. Adjustment knob

P. Identication ring

C. Shaft

D. Ejector

E. Tip

2 | AXYGEN AXYPET PIPETTORS

The pipettor design allows the user to lock the volume

setting by sliding the locking ring upwards.

The symbols printed on the handle show locked and

unlocked positions. The volume range of each pipettor

model is shown on the pipetting pushbutton (A1).

Liquids are aspirated into disposable pipet tips attached

to the pipettor shaft. Disposable pipet tips ensure

maximum safety and eliminate the possibility of cross-

contamination of the liquid samples. To protect the user

from contaminated pipet tips, the pipettor is fitted with a

built-in tip ejector (D).

Single-channel Pipettor Ejector

The ejector length can be adjusted to accommodate all

common brands of pipet tips. When using narrow tubes,

it may be necessary to remove the tip ejector. This is done

simply by pulling down.

Adjusting the Tip Ejector Length

2 to 1,000 μL Pipettors

The “H“ spacers included with

each pipettor, allow the ejector

length to be adjusted by +1 mm

or +2 mm.

An ”H0” spacer is fitted as

standard. The outside shape

of the spacer identifies the

adjustment.

Instruction Manual | 3

5,000 and 10,000 μL Pipettors

The length of tip ejector is adjusted by screwing in or

screwing out the tip ejector arbor with a small screwdriver.

Turn the screwdriver counterclockwise to increase the length

of the tip ejector, and clockwise to reduce the length of the

tip ejector. The ejector can be adjusted by up to 5 mm.

If the ejector adjustment described above is not sufficient

or the diameter of the ejector opening is too large to eject

the tip, it will be necessary to put the ejector cap “M” onto

the ejector.

2 and 10 μL Pipettors

The 2 μL to 10 μL pipettors may require the use of an

ejector cap “M” to efficiently eject certain brands of pipet

tips. Simply place the cap (supplied with the pipettor)

on the bottom of the pipettor shaft and slide the cap

upwards until it surrounds the bottom of the tip ejector.

5,000 μL: L + 6 mm

10,000 μL: L + 10 mm

4 | AXYGEN AXYPET PIPETTORS

8-channel and 12-channel Pipettor Ejector

The 8-channel and 12-channel pipettor construction

allows the lower 8-channel and 12-channel module to be

rotated 360°. This allows the user to disable the ejector to

avoid accidental use and to change its position to make

filling of microplates more comfortable and convenient. It

is recommended to turn the module clockwise.

The pipettor is a high quality instrument which offers

excellent accuracy and precision.

The accuracy and precision (repeatability) of the liquid

volume depends on the quality of pipet tips used. The

values for accuracy and precision shown in the table below

were obtained using Axygen® pipet tips.

Model

Volume

Range

(μL) Cat. No.

Volume

(μL)

Accuracy

(%)

Precision

(%)

Fit to Tips

Without

Filter

(μL)

Single-channel Pipettors

2 AP-2

Min. 0.1 ±40.0 ≤12.0

101 ±2.7 ≤1.30

Max. 2 ±1.5 ≤0.70

10 AP-10

Min. 0.5 ±4.0 ≤2.80

105 ±1.0 ≤0.60

Max. 10 ±0.5 ≤0.40

20 AP-20

Min. 2 ±3.0 ≤1.50

20010 ±1.0 ≤0.50

Max. 20 ±0.8 ≤0.30

50 AP-50

Min. 5 ±2.5 ≤2.00

200

25 ±1.0 ≤0.60

Max. 50 ±0.8 ≤0.40

100 AP-100

Min. 10 ±1.6 ≤0.80

20050 ±0.8 ≤0.24

Max. 100 ±0.8 ≤0.20

200 AP-200

Min. 20 ±1.2 ≤0.60

200100 ±0.8 ≤0.25

Max. 200 ±0.6 ≤0.20

250 AP-250

Min. 50 ±1.0 ≤0.40

300125 ±0.8 ≤0.30

Max. 250 ±0.6 ≤0.30

1,000 AP-1000

Min. 100 ±1.6 ≤0.40

1,000500 ±0.7 ≤0.20

Max. 1,000 ±0.6 ≤0.15

Instruction Manual | 5

Model

Volume

Range

(μL) Cat. No.

Volume

(μL)

Accuracy

(%)

Precision

(%)

Fit to Tips

Without

Filter

(μL)

Single-channel Pipettors (continued)

5,000 AP-5000

Min. 1,000 ±0.6 ≤0.25

5,0002,500 ±0.6 ≤0.20

Max. 5,000 ±0.5 ≤0.15

10,000 AP-10ML

Min. 1,000 ±2.5 ≤0.60

10,0005,000 ±0.8 ≤0.30

Max. 10,000 ±0.5 ≤0.20

8-channel and 12-channel Pipettors

8 - 10

12 - 10

AP-8-10

AP-12-10

Min. 0.5 ±10.0 ≤8.0

105 ±4.0 ≤2.0

Max. 10 ±2.0 ≤1.2

8 - 50

12 - 50

AP-8-50

AP-12-50

Min. 5 ±4.0 ≤2.5

20025 ±3.0 ≤1.2

Max. 50 ±1.6 ≤0.6

8 - 200

12 - 200

AP-8-200

AP-12-200

Min. 20 ±3.0 ≤1.5

200100 ±1.5 ≤0.8

Max. 200 ±1.0 ≤0.6

8 - 300

12 - 300

AP-8-300

AP-12-300

Min. 50 ±1.6 ≤1.5

300150 ±1.2 ≤1.0

Max. 300 ±1.0 ≤0.6

The accuracy and precision are obtained gravimetrically using

Axygen® pipet tips, performing at least 10 measurements

of distilled water at a reference temperature of 20°C ± 1°C

according to EN ISO 8655 standard.

2. Setting the Volume

The volume of the pipettor is set by turning the pipetting

pushbutton (A2) or the black adjustment knob (B).

The volume display consists of three numbers and is

read from top to bottom. The three numbers indicate the

volume selected and are colored black and red.

Red numbers represent decimal fractions for microvolume

pipettors or milliliters for macrovolume pipettors.

6 | AXYGEN AXYPET PIPETTORS

An example for each of these pipettors is given below.

Model Volume

Range (μL)

Counter

Readings

Set

Volume Increment

Single-channel Pipettors

1.25 μL 0.002 μL

7.5 μL 0.02 μL

12.5 μL 0.02 μL

38.0 μL 0.2 μL

100

75.0 μL 0.2 μL

200

125.0 μL 0.2 μL

250

245.0 μL 0.2 μL

1,000

750.0 μL 2 μL

5,000

1.25 mL 10 μL

10,000

7.5 mL 20 μL

Instruction Manual | 7

Model Volume

Range (μL)

Counter

Readings

Set

Volume Increment

8-channel and 12-channel Pipettors

8 - 10

12 - 10

3.5 μL 0.02 μL

8 - 50

12 - 50

6.5 μL 0.10 μL

8 - 200

12 - 200

85.0 μL 0.20 μL

8 - 300

12 - 300

250.0 μL 1.0 μL

To attain the maximum accuracy, the set volume must be

approached from a higher value.

If the desired value is lower than the previous value, adjust

the value down to a one-third turn above the required

setting. If the desired value is higher than the previous

value, adjust the value to a one-third turn above the

required value. The setting should then be performed in

the following manner:

• Slowly turn the pipetting pushbutton or the black

adjustment knob to the required volume.

• If the knob is accidentally turned too far below the

required value, the process must be repeated.

• Set the locking ring into the upper position following

the volume adjustment, thus locking the knob and

preventing accidental volume change.

8 | AXYGEN AXYPET PIPETTORS

3. Aspiration and Dispensing Instructions

Single-channel Pipettors

Place a pipet tip on the shaft of the pipettor. See Section 6

for the appropriate tip. Press the pipet tip on firmly using a

slight twisting motion. This will ensure an airtight seal.

8-channel and 12-channel Pipettors

To put pipet tips on the shafts: hold the pipettor vertically

and press it against the tips in the rack box until the shafts

retract approximately 1.5 mm into the manifold. The

suspension system of the shafts ensures even and tight

sealing of the pipet tips. The rocking movement does not

have to be performed to seal the pipet tips tightly.

NOTE: Never handle a liquid with a pipettor which has not

been fitted with a pipet tip.

Aspiration

1. Press the pushbutton to the first positive stop. Holding

the pipettor vertically, immerse the pipet tip into the

sample liquid. The depth to which the pipet tip is

immersed in the sample liquid depends on the model.

Model Volume Range (μL) Immersion Depth (mm)

2, 10 ≤1

20, 50, 100 2 - 3

200, 250, 300, 1,000 2 - 4

5,000 3 - 6

10,000 5 - 7

1234

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