Biosan PST-60HL Manual de usuario

DOMINIQUE DUTSCHER SAS

Contents

1. Safety Precautions ....................................................................................... 4

2. General information ...................................................................................... 6

3. Getting started .............................................................................................. 7

4. Operation ...................................................................................................... 8

5. Calibration .................................................................................................. 10

6. Specifications ............................................................................................. 12

7. Maintenance ............................................................................................... 13

8. Warranty and Claims .................................................................................. 14

9. Declaration of Conformity ........................................................................... 15

DOMINIQUE DUTSCHER SAS

1. Safety Precautions

The following symbols mean:

Caution! Make sure you have fully read and understood the present

Manual before using the equipment. Please pay special

attention to sections marked by this symbol.

Caution! Surfaces can become hot during use.

GENERAL SAFETY

Use only as specified in the Operating Manual provided.

Save the unit from shocks or falling.

Store and transport the unit in a horizontal position (see package label).

After transportation or storage and before connecting to electric circuit, keep

the unit under room temperature for 2-3 hrs.

Before using any cleaning or decontamination methods except those

recommended by the manufacturer, check with the manufacturer that the

proposed method will not damage the equipment.

Do not make modifications to the design of the unit.

ELECTRICAL SAFETY

Connect only to the external power supply unit with voltage corresponding to

that on the serial number label.

Use only the external power supply unit provided with this product.

Ensure that the switch and external power supply are easily accessible during

use.

Do not plug the unit into an ungrounded power socket, and do not use an

ungrounded extension lead.

Disconnect the unit from electric circuit before moving. Switch the unit off and

disconnect the power cord plug from power socket to disconnect the unit from

electric circuit.

hazardous material is spilt on or penetrates into the equipment. If liquid

penetrates into the unit, disconnect it from electric circuit and have it checked

by a repair and maintenance technician.

Do not operate the unit in premises where condensation can form. Operating

conditions of the unit are defined in the Specifications section.

DOMINIQUE DUTSCHER SAS

DURING OPERATION

Do not leave the operating unit unattended.

Do not impede the platform motion.

Do not operate the unit in environments with aggressive or explosive chemical

mixtures. Please contact manufacturer for possible operation of the unit in

specific atmospheres.

Do not operate the unit if it is faulty or has been installed incorrectly.

Do not fill in the microplates after they have been inserted in the unit.

Do not use outside laboratory rooms.

Do not check the temperature by touch. Use a thermometer.

BIOLOGICAL SAFETY

hazardous material is spilled on or penetrates into the equipment.

DOMINIQUE DUTSCHER SAS

2. General information

PST-60HL / PST-60HL-4 Plate Shaker-Thermostat is designed for shaking 1

to 4 standard 96-well plates in the thermal regulation mode.

Plate Shaker-Thermostat was designed using the multi-system principle,

which allows using it as three independent devices:

1) incubator for lasting incubation of micro quantities (insect, plant cell cultures,

etc.) in plates;

2) plate shaker for operation in the cold room or other conditions, which do not

require temperature stabilization;

3) microplate thermo-Shaker for immunochemistry and molecular diagnostics,

where the requirements to the result reproducibility and thus to the precise

method regulation are particularly high.

A distinctive feature of Biosan plate thermoshakers is the patented two-side

plate heating that allows to achieve full correspondence of the set and actual

temperature in the plate wells.

PST-60HL/PST-60HL-4 Plate Shaker-Thermostat provides:

soft or intensive sample shaking;

rotation speed regulation, stabilization and indication;

even shaking amplitude throughout Shaker-Thermostat platform;

required operation time setting and indication;

automatic stopping of the platform movement after the set time expires;

current operation time indication;

setting and indication of the required temperature;

fault automatic diagnostics (temperature sensor, platform heating, lid heating

and other errors).

The device can be used in:

cytochemistry

for in situ reactions;

immunochemistry

for immunofermentative reactions;

biochemistry

for enzyme and protein analysis;

molecular biology

for matrix analysis, DNA and RNA analyses.

The maximum guaranteed number of diagnostic cycles in the Plate Shaker-

Thermostat mode, which require 15-30 min work in one cycle, is 7000-14000 times.

External 12V power supply unit is used to power the device. It makes it safe

to work in the cold room, where condensation may cause leakage current from

electric circuit.

DOMINIQUE DUTSCHER SAS

3. Getting started

3.1. Unpacking.

Remove packing materials carefully and retain for them future shipment or

storage of the unit.

Examine the unit carefully for any damage incurred during transit. The

warranty does not cover in-transit damage.

Warranty covers only the units transported in the original package.

3.2. Complete set. Package contents:

- PST-60HL / PST-60HL-4 Plate Shaker-Thermostat .............................. 1 pce

- spare rubber belt ...................................................................................2 pcs

- external power supply ............................................................................ 1 pce

- power cord ............................................................................................. 1 pce

- Operating manual, Certificate ..............................................................1 copy

3.3. Set up:

- place the unit upon even horizontal non-flammable surface away from any

flammable materials (not less than 30 cm);

- remove protective film from the display;

- plug the external power supply unit into the socket at the rear side of the unit

and position the unit so that there is easy access to the power switch and

the external power supply unit.

DOMINIQUE DUTSCHER SAS

4. Operation

Recommendations during operation

For proper mixing, it is not recommended to fill microplates for more than 75%

of their nominal volume.

Seal the microplates with an appropriate adhesive film to avoid spilling the

sample.

Fig.1 Control panel

4.1. Connect the external power supply unit to a grounded power socket and set

the power switch located on the rear panel of the unit to position I (ON).

4.2. The display will turn on. Upper line (Set) shows time, speed and temperature

set earlier. Lower line (Actual) shows current readings of the same

according to the temperature set in the upper line). The time of temperature

stabilisation depends on the initial temperature.

4.3. Setting the parameters. Use the readings in the upper line of the display (Set),

while setting the necessary parameters. Pressing the key for more than 3 s

will increase the increment.

4.3.1. Setting time (TIME) he required

working time interval in hours and minutes (increment 1 min).

4.3.2. Setting speed (RPM). uired

speed (increment 10 RPM).

4.3.3.

Caution! The platform heating can be turned off only by setting the

- - set point).It can be used in cold rooms as a mixing

device without thermal regulation in this mode.

4.4. Program execution. After the thermal stabilisation of the unit (when the set

and current temperature readings become the same):

4.4.1. Microplate fixation:

PST-60HL: Place microplates on the platform and fix it with the special

push-down clip by pressing it against the plate covers.

PST-60HL-4: Unscrew the fixation screws. Place microplates on the

platform and fix them by tightening the fixation screws.

DOMINIQUE DUTSCHER SAS

Caution! For model PST-60HL: Load only pairs of microplates for best

fixation.

Correct loading

Wrong loading

Caution! Do not fill in the microplates after they have been inserted in

the unit.

Caution! For model PST-60HL-4: The microplate fixation screws must

always be tightened to avoid damage. Tighten the fixation

screws completely when microplates are removed from or

placed on the platform. Do not close the lid if the microplate

fixation screws are not tightened to avoid damage.

4.4.2. Press the RPM-RUN/STOP key (Fig. 1/4). The platform will start rotating and

the timer indicator will start counting up the time interval (with 1 min precision).

4.4.3. After finishing the program, the platform motion will stop and the timer will

show the flashing reading STOP accompanied by the repetitive sound signal

until the RPM-RUN/STOP key is pressed.

4.5. If the working time is not set (or is reset) and the timer indicator in the upper line

shows 00:00, pressing the RPM-RUN/STOP key will start continuous operation

of the Thermo-shaker (timer indicator will start counting up the time interval in

the lower line (Actual)) until the RPM-RUN/STOP key is pressed again.

Please note! The platform temperature will be constantly maintained in

accordance with the set temperature. This allows using the

device again without pre-heating.

4.6. The timer can be reset during operation if required. Press the TIME-

RUN/STOP key once (Fig. 1/5) to stop the timer. Press the TIME-RUN/STOP

key again to restart the timer.

4.7. The platform motion can be stopped at any time by pressing the RPM-RUN

/STOP key. In this case the program realisation will stop and the timer will

switch into the STOP mode saving previously set time. Press the RPM-

RUN/STOP key to repeat the operation with the same time and speed.

Caution! At the end of the set time period the platform movement is

stopped automatically, but the heating can be stopped only by

, lower

button) till the OFF sign appears in the upper part of the display.

4.8. After finishing the operation set the power switch, located on the rear panel of

the unit, in position O (Off) and disconnect the external power supply from

electric circuit.

DOMINIQUE DUTSCHER SAS

5. Calibration

Attention! Calibration is available for models of version V.6AW (PST-

60HL) and V.4AW (PST-60HL-4).

5.1. The device is pre-calibrated at the factory (calibrating coefficient is 1.000) for

operation with temperatures, measured by a sensor, installed in the heating

block.

5.2. To enter the calibration coefficient, hold the Stop key (fig. 1/8) pressed for

more than 8 s to activate calibration mode. The calibration coefficient will be

shown on the display (fig. 2/1).

5.3. Restoring factory settings. Temp keys

(fig. 1/6) as shown on Fig. 2/1 to restore the factory settings. Press the Run

key (fig. 1/7) once to save the changes and exit the calibration mode.

Note. Coefficient value changes are recommended after the unit has

5.4.1. Calibration procedure.

spectrocells, placed into the block sockets.

5.4.2.

Fig.2 Control panel in calibration mode

5.4.3. After the unit reaches the set temperature (when the set and current

temperature readings equal) leave the unit for 30 minutes for thermal

stabilization.

5.4.4.

s actual temperature is 40

correction.

5.4.5. Hold Stop key (fig. 1/8) pressed for more than 8 s to activate calibration mode.

The following parameters will be shown on the display (fig. 2).

DOMINIQUE DUTSCHER SAS

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