biochrom Anthos Fluido 2 Manual de usuario
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 1
Biochrom Anthos Fluido 2
Microplate Washer
User’s Manual
Biochrom Ltd
22 Cambridge Science Park,
Milton Rd, Cambridge CB4 0FJ UK
Tel: +44 (0)1223 423723
Fax: +44 (0)1223 420164
www.biochrom.co.uk
Biochrom Ltd
22 Cambridge Science Park,
Milton Rd, Cambridge CB4 0FJ UK
Tel: +44 (0)1223 423723
Fax: +44 (0)1223 420164
www.biochrom.co.uk
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 2
Table of Contents
1Introduction....................................................................................................... 4
2Warnings and Hazards....................................................................................... 5
2.1.1 Safety symbols ..................................................................................................................................... 6
3Precautions........................................................................................................ 8
3.1 Cleaning the instrument................................................................................................................................ 8
4Specifications..................................................................................................... 9
4.1 Metrological specification............................................................................................................................. 9
4.1.1 Rated operating conditions ....................................................................................................................... 9
4.1.1 Limits of error and tolerance limits........................................................................................................... 9
4.2 General technical data................................................................................................................................... 9
4.2.1 Dimensions.............................................................................................................................................. 9
4.2.2 Power supply............................................................................................................................................ 9
4.2.3 Interfacing.............................................................................................................................................. 10
4.2.4 Options .................................................................................................................................................. 10
4.3 Cleaning..................................................................................................................................................... 10
4.4 General features ......................................................................................................................................... 10
4.5 Standard delivery content ........................................................................................................................... 12
4.6 Warnings and directions ............................................................................................................................. 13
4.7 Unpacking Procedure ................................................................................................................................. 14
4.8 Installation requirements, Environmental operating conditions .................................................................... 14
4.9 Instrument installation procedure ................................................................................................................ 15
4.10 Re-packing to prevent damage during transport........................................................................................... 17
5Theory of Operation......................................................................................... 18
5.1 Introduction................................................................................................................................................ 18
5.2 Main components....................................................................................................................................... 18
5.3 Hydraulic system........................................................................................................................................ 18
5.3.1 Dispensing system.................................................................................................................................. 18
5.3.2 Aspirating system................................................................................................................................... 18
6Warranty......................................................................................................... 20
6.1 Liability ..................................................................................................................................................... 20
7Software Principle............................................................................................ 21
7.1 Software Navigation................................................................................................................................... 21
7.2 Programming Sequence .............................................................................................................................. 23
7.3 Menus........................................................................................................................................................ 23
7.3.1 Run Procedure........................................................................................................................................ 25
7.3.2 Prime / Rinse.......................................................................................................................................... 26
7.3.3 Define Procedure.................................................................................................................................... 27
7.3.4 Define Cycle .......................................................................................................................................... 28
7.3.5 Define Plate ........................................................................................................................................... 29
7.3.6 Setup...................................................................................................................................................... 30
7.3.7 Service................................................................................................................................................... 31
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 3
7.4 Description of the key functions.................................................................................................................. 32
8Start-up and system setup ................................................................................ 33
8.1 Start-Up ..................................................................................................................................................... 33
8.2 Control of the system setup......................................................................................................................... 33
9Programming................................................................................................... 35
9.1 Run Procedure............................................................................................................................................ 35
9.2 Prime/Rinse Menu...................................................................................................................................... 38
9.2.1 Manual Prime/Rinse............................................................................................................................... 38
9.2.2 Automatic Prime/Rinse........................................................................................................................... 39
9.2.3 Periodic Prime/Rinse.............................................................................................................................. 40
9.3 Define Procedure Menu.............................................................................................................................. 42
9.4 Define Cycle Menu..................................................................................................................................... 47
9.5 Define Plate Menu...................................................................................................................................... 52
9.6 Setup Menu................................................................................................................................................ 65
9.7 Service Menu ............................................................................................................................................. 69
10 ERROR MESSAGES....................................................................................... 70
10.1.1 Duplicate Name ................................................................................................................................. 70
10.1.2 Wrong Manifold................................................................................................................................. 70
10.1.3 No Cycle Defined............................................................................................................................... 70
10.1.4 No Plate Defined........................................................................................................................... 70
10.1.5 Waste Full.......................................................................................................................................... 71
10.1.6 Liquid Empty................................................................................................................................... 71
10.1.7 Stop Pressed....................................................................................................................................... 72
10.1.8 Plate Transport Error.......................................................................................................................... 72
10.1.9 Manifold Transport Error ................................................................................................................... 72
11 Maintenance.................................................................................................... 73
11.1 Recommended regular inspection ............................................................................................................... 73
11.2 Approved parts........................................................................................................................................... 73
11.3 Exchanging a Fuse...................................................................................................................................... 73
11.4 Cleaning the instrument.............................................................................................................................. 74
11.5 Disinfection Procedure ............................................................................................................................... 74
12 Intended Use.................................................................................................... 76
12.1 Quality control ........................................................................................................................................... 76
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 4
1Introduction
The Fluido 2 is a fully automated, programmable 96-well microplate
washer with 8- or 12-way manifolds. An optional 16-channel manifold
allows washing of 384-well microplates.
The Fluido 2 is designed around your application. It incorporates state of
the art technology and software both necessary to allow optimum
performance and easy use.
Easy operation through the menu structure of the on-board software. The
display and the keyboard provides clear access to all features of the
flexible software
The Fluido 2 can be operated with up to 3 different wash liquids to be
selected by software.
An easy plate setup is provided to optimise the movement of the plate and
the manifold according to the different dimensions of different plate types.
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 5
2Warnings and Hazards
General:
If inflammable, toxic or biologically hazardous substances are used
when operating the equipment, please observe the instructions and
precautions enclosed with such substance.
Never spill fluids in or on the equipment.
Wash your hands thoroughly after handling test fluids.
If equipment has been in contact with hazardous substances, it must be
disinfected prior to shipment in accordance with the effective
provisions.
Do not touch the plate during movement of the plate transport (risk of
injury).
The equipment may not be opened other than as described in chapter
11 Maintenance, since it contains live parts (danger of life).
Electrical:
Voltages dangerous to human life are present in this device. Before
removing any covers disconnect the device from the power source.
Ensure that the power cord supplied with the unit is used.
The power cord may only be inserted in a socket outlet provided with a
protective ground (earth) contact. The protective action must not be
negated by use of an extension cord without a protective grounding
contact.
Do not replace fuses without first removing the main power cord.
Ensure that only fuses with the required rated current and of the
specified type are used for replacement. The use of makeshift fuses
and the short-circuiting of fuse-holders is prohibited.
When the apparatus is connected to the main power source, the
opening of the covers or removal of components is likely to expose life
parts. The device shall be disconnected from all voltage sources before
it is opened for adjustment or repair.
Any adjustment or repair of the opened apparatus under voltage should
be avoided, but, if necessary, it must be carried out by qualified service
personnel who are aware of the hazards involved.
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 6
Magnetic:
The removable plate carriage of this instrument has a very strong
permanent magnet mounted. Be sure to never bring any other magnets
or Ferro-magnetic parts near to the magnet of the plate carriage (risk of
injury).
Never place credit cards or IDs or any other items using magnet-code
(floppy disks, audio- or video tapes, ...) near to the magnet of the plate
carriage (risk of data loss).
Be careful when placing the plate carriage onto other sensitive medical
devices, which may be influenced by magnetic fields.
2.1.1 Safety symbols
The following safety symbols may be found in several locations on the instrument. Only persons
who fully understand the safety precautions and recognize shock hazards should operate this
instrument.
~Alternating current
ON
OFF
In Vitro Diagnostic Device
Protective ground terminal
Caution, see enclosed document
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 7
The symbol on the product indicates that this product shall not be
treated as household waste. Instead it shall be handed over to the
applicable collection point for the recycling of electrical and electronic
equipment.
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 8
3Precautions
3.1 Cleaning the instrument
This instrument is a precision instrument and it requires regular cleaning
to ensure the continued precision.
Liquid Spills
If any liquid is spilled on the instrument, it should be IMMEDIATELY
removed so that the liquid does not attack the surface of the housing.
Regular cleaning
The housing of the instrument should be cleaned regularly with a mild
household cleaning agent.
Warning: Do not use aggressive solutions!
The area surrounding the plate carrier should be cleaned with a dry cloth.
Fittings and tubing
The usual wash buffer tends to crystallize easily on air and can then block
tubing and fittings. Use the automatic rinse program daily and do not leave
the tubing filled with wash buffer for an extended period of time.
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 9
4Specifications
4.1 Metrological specification
4.1.1 Rated operating conditions
Warming-up time: Ready for operation immediately after
switching-on.
Operating voltage: 90V - 130V,
180V –250V autosensing
Fuses (user exchangeable): 2 pcs. 2.5 A TH250VAC
Built in Fuse (on power supply): 1 pcs. F 2A, 250V, fa
Display: 2x16 characters display (with backlight)
Keyboard: Keyboard with 5 function keys
Ambient temperature: 15°C - +40°C (operation)
-25°C - +50°C (storage)
Relative humidity: 15 - 85% non-condensing (operation)
< 95% non-condensing (storage)
Air pressure tolerance: 54.000 - 106.000 Pascal
Maximum altitude (operation): up to 5000 m
4.1.1 Limits of error and tolerance limits
Dispense volume: wash liquid volume adjustable from 50 -
2000µl in increments of 50µl
Dispensing accuracy:
Residual volume:
The tolerances of the measuring devices are based on the manufacturer‟s
information.
4.2 General technical data
4.2.1 Dimensions
Weight: 6,2 kg net
Dimensions (width x length x height): 21 cm x 46 cm x 21 cm
4.2.2 Power supply
Consumption: 80VA (maximum)
Biochrom Anthos Fluido-2 Microplate Washer v2.0 Page 10
Operating voltage: 90-130V, 180-250VAC (autosensing),
frequency range: 47 - 63 Hz
4.2.3 Interfacing
Serial interface (RS232 9-pin male connector) for remote control of basic
wash functions, instrument status and setup by external PC (with null-
modem serial cable used for PC connection).
4.2.4 Options
liquid level sensors for wash and waste bottles
8- and 12-way manifold for 96-well plates
16-way manifold for 384-
Serial interface cable
4.3 Cleaning
This instrument is a precision instrument and it requires regular cleaning
to ensure the continued precision.
Liquid spillage
If any liquid is spilled on the instrument, it should be IMMEDIATELY
removed so that the liquid does not attack the surface of the housing.
Regular cleaning
The housing of the instrument should be cleaned regularly with a mild
household cleaning agent.
Warning: Do not use aggressive solutions!
The area surrounding the plate carrier should be cleaned with a dry cloth.
Fittings and tubing
The usual wash buffer tends to crystallize easily on air and can then block
tubing and fittings. Use the automatic rinse program daily and do not leave
the tubing filled with wash buffer for an extended period of time.
4.4 General features
The Fluido 2 incorporates state of the art technology and software both
necessary to allow optimum performance and easy use.
The Fluido 2 is designed around your application.
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