Biochrom Anthos 2010 Manual de usuario

July 2010 Anthos 2010 Quick Start Guide Version 1.0

BIOCHROM ANTHOS 2010 MICROPLATE READER

QUICK START GUIDE

1. To turn on the instrument:

Connect instrument to a power source using the appropriate power cord. Turn on the

instrument using the switch at the back of the instrument.

Check user’s manual for important safety information.

2. To connect the instrument to a PC:

Connect to the PC via serial port to serial port or a serial to USB port adaptor. Determine the

communication port (com) used by the instrument. In the Start menu of the PC, go to

Control Panel\System\Hardware\Device Manager\Ports.

3. To connect instrument to ADAP software:

Insert the CD supplied with the instrument into PC, install ADAP. Open ADAP. ADAP will

prompt for a user ID and password. Login using the pre-set ID and password:

sadmin/sadmin. Once logged as sadmin, specific user IDs, passwords and administrative

rights may be set.

Select Setup/Instrument in the task bar. A dialogue box will open:

Under the Instrument tab:

In Baudrate, select 9600

In COM Port, select port or Auto

Sense

In Instrument Type, select 2010 in

the drop-down menus.

To confirm that the instrument is connected with the computer, select the Read

Configuration button. The serial number of the instrument should now appear in the

Setup/Instrument dialogue box along with compatible plate types. Select File\Save to save

settings.

July 2010 Anthos 2010 Quick Start Guide Version 1.0

4. To measure a plate:

Go to Reading/Quick or the R button in the menu bar.

In the Quick-Read dialogue box: Confirm

that the correct format and plate type are

selected.

a. Select All in Measurement Position

to read the entire plate.

b. Select Endpoint Photometric for

basic readings using a measurement

and reference filter.

c. Select the measurement filter and

the reference filter from the drop-

down menu.

Note: It is important to use a reference filter to account for optical inference from the plate.

Place plate in the plate transporter. Select Start. Absorbance measurements will appear in the

open matrix in ADAP. When prompted, enter a plate ID. Data can be exported to data analysis

software using the Copy icon. Data will paste as a matrix with filter wavelength, with time and

date.

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