Zeiss LSM 880 Manual de usuario

ZEISS LSM 880 INVERTED (i880) QUICK START GUIDE

The Zeiss LSM880 is a laser scanning confocal microscope with full confocal and multiphoton capability. The

confocal detectors are spectrally resolved and can be used with either the standard (single photon) excitation

lines or the multiphoton excitation lines. The non-descanned (NDD) detectors can only be used with multiphoton

excitation.

INSTRUMENT OVERVIEW

AND USER RESOURCES

FULL TURN ON SEQUENCE (FIRST ONE ON)

1) If the computer is off, turn it on and let it boot.

2) Turn on the main switch.

3) Turn on the Systems/PC switch. (When

touchscreen has booted, adjust heating and

CO2 under Microscope->Inkubation if

applicable.)

4) Turn on the Components switch. Wait for the

RTC to boot (see under table).

5) Log in to the computer.

6) Start Zen and select Start System Button.

7) Turn on laser lines you will be using. (If you are

using multiphoton excitation, turn on both lines

on even if you are just using one. Note that the

Ar and multiphoton lines take a while to turn on.

You can follow the warm-up progress by

selecting the laser and the Laser Properties

below)

FULL TURN OFF SEQUENCE (LAST ONE ON)

9) Save your data and transfer it to the fileshare.

8) Remove your sample and clean off your

objective. (If using incubation, turn temp and

CO2 regulation off and close CO2 tank using

main gray valve only.)

7) Turn off lasers.

6) Exit Zen.

5) Log out of the computer.

4) Turn off the Components switch.

3) Turn off the Systems/PC switch.

2) Wait a few minutes for the Ar laser to cool.

Turn off the main switch.

1) Do not turn off computer.

PARTIAL TURN OFF SEQUENCE

(Someone else is scheduled after your time slot)

9) Save your data and transfer it to the fileshare.

8) Remove your sample and clean off your

objective. (If using incubation, turn temp and

CO2 regulation off and close CO2 tank using

gray handle only.)

7) Turn off lasers.

6) Exit Zen.

5) Log out of the computer.

PARTIAL TURN ON SEQUENCE

(Microscope already on)

5) Log in to the computer.

6) Start Zen and select Start System Button.

7) Turn on laser lines you will be using. (If you are

using multiphoton excitation, turn on both lines

on even if you are just using one. Note that the

Ar and multiphoton lines take a while to turn on.

You can follow the warm-up progress by

selecting the laser and the Laser Properties

below)

LASER LINES

STANDARD OBJECTIVE SPECIFICATIONS

Mag

Immersion

medium

CS thickness

(mm)

WD (mm)

Objective Type

Trans > 0.5

10X

0.3

Air

0.17

5.2

EC Plan-Neofluar

350-1200nm

20X

0.5

Air

0.17

2.0

EC Plan-Neofluar

350-1250nm

32X

0.85

Water

0-0.17

1.1

C-Achroplan

450-1300nm

40X

1.1

Water

0.14-0.19

0.62

LD C-Apochromat

350-1150nm

40X

1.4

Oil

0.17

0.13

Plan-Apochromat

380-1200nm

10X

0.45

Water

0.17

1.8

C-Apochromat

350-1200nm

63X

0.75

Air

0-1.5

1.7

(at 0.75 CS)

LD Plan-Neofluar

350-950nm

NDD DETECTOR FILTERS

*Do not use any laser lines <700nm with the NDD detectors!

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