WATERS CORPORATION ACQUITY UPLC Manual de funcionamiento

ACQUITY UPLC

TUV Detector

Operator’s Overview and Maintenance Guide

Revision A

DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY

FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

The information in this document is subject to change without notice and

should not be construed as a commitment by Waters Corporation. Waters

Corporation assumes no responsibility for any errors that may appear in this

document. This document is believed to be complete and accurate at the time

of publication. In no event shall Waters Corporation be liable for incidental or

consequential damages in connection with, or arising from, its use.

Trademarks

ACQUITY, ACQUITY UPLC, UPLC, Waters PIC, and Waters are registered

trademarks, and Empower, MassLynx, and “THE SCIENCE OF WHAT’S

POSSIBLE.” are trademarks of Waters Corporation.

PEEK is a trademark of Victrex Corporation.

Teflon is a registered trademark of E. I. du Pont de Nemours and Company.

TRITON is a trademark of Union Carbide Corporation.

Other registered trademarks or trademarks are the sole property of their

owners.

iii

Customer comments

Waters’ Technical Communications department invites you to tell us of any

errors you encounter in this document or to suggest ideas for otherwise

improving it. Please help us better understand what you expect from our

documentation so that we can continuously improve its accuracy and

usability.

We seriously consider every customer comment we receive. You can reach us

at [email protected].

Contacting Waters

Contact Waters®with enhancement requests or technical questions regarding

the use, transportation, removal, or disposal of any Waters product. You can

reach us via the Internet, telephone, or conventional mail.

Safety considerations

Some reagents and samples used with Waters instruments and devices can

pose chemical, biological, and radiological hazards. You must know the

potentially hazardous effects of all substances you work with. Always follow

Good Laboratory Practice, and consult your organization’s safety

representative for guidance.

When you develop methods, follow the “Protocol for the Adoption of Analytical

Methods in the Clinical Chemistry Laboratory,” American Journal of Medical

Technology, 44, 1, pages 30–37 (1978). This protocol addresses good operating

procedures and the techniques necessary to validate system and method

performance.

Waters contact information

Contacting medium Information

Internet The Waters Web site includes contact

information for Waters locations worldwide.

Visit www.waters.com.

Telephone and fax From the USA or Canada, phone 800

252-HPLC, or fax 508 872 1990.

For other locations worldwide, phone and fax

numbers appear in the Waters Web site.

Conventional mail Waters Corporation

34 Maple Street

Milford, MA 01757

USA

Considerations specific to the ACQUITY TUV detector

High voltage hazard

Safety advisories

Consult the Safety Advisories section on page 62 for a comprehensive list of

warning and caution advisories.

Warning: To avoid electric shock, do not remove the TUV detector’s

protective panels. The components within are not user-serviceable.

Operating the ACQUITY TUV detector

When operating this instrument, follow standard quality-control (QC)

procedures and the guidelines presented in this section.

Applicable symbols

Audience and purpose

This guide is intended for personnel who install, operate, and maintain

ACQUITY TUV detectors. It gives an overview of the instrument’s technology

and operation.

Intended use of the ACQUITY TUV detector

The Waters ACQUITY TUV detector is for research use only.

Symbol Definition

Authorized representative of the European

Community

Confirms that a manufactured product complies

with all applicable European Community

directives

Australia C-Tick EMC Compliant

Confirms that a manufactured product complies

with all applicable United States and Canadian

safety requirements

This product has been tested to the requirements

of CAN/CSA-C22.2 No. 61010-1, second edition,

including Amendment 1, or a later version of the

same standard incorporating the same level of

testing requirements

Consult instructions for use

vii

Calibrating

To calibrate LC systems, follow acceptable calibration methods using at least

five standards to generate a standard curve. The concentration range for

standards must include the entire range of QC samples, typical specimens,

and atypical specimens.

When calibrating mass spectrometers, consult the calibration section of the

operator’s guide for the instrument you are calibrating. In cases where an

overview and maintenance guide, not operator’s guide, accompanies the

instrument, consult the instrument’s online Help system for calibration

instructions.

Quality-control

Routinely run three QC samples that represent subnormal, normal, and

above-normal levels of a compound. Ensure that QC sample results fall within

an acceptable range, and evaluate precision from day to day and run to run.

Data collected when QC samples are out of range might not be valid. Do not

report these data until you are certain that the instrument performs

satisfactorily.

When analyzing samples from a complex matrix such as soil, tissue,

serum/plasma, whole blood, and other sources, note that the matrix

components can adversely affect LC/MS results, enhancing or suppressing

ionization. To minimize these matrix effects, Waters recommends you adopt

the following measures:

• Prior to the instrumental analysis, use appropriate sample

pretreatment such as protein precipitation, liquid/liquid extraction

(LLE), or solid phase extraction (SPE) to remove matrix interferences.

• Whenever possible, verify method accuracy and precision using

matrix-matched calibrators and QC samples.

• Use one or more internal standard compounds, preferably isotopically

labeled analytes.

viii

Authorized representative information

Authorized representative

ISM classification

ISM Classification: ISM Group 1 Class B

This classification has been assigned in accordance with CISPR 11 Industrial

Scientific and Medical (ISM) instruments requirements. Group 1 products

apply to intentionally generated and/or used conductively coupled

radio-frequency energy that is necessary for the internal functioning of the

equipment. Class B products are suitable for use in both commercial and

residential locations and can be directly connected to a low voltage,

power-supply network.

Waters Corporation (Micromass UK Limited) is

registered in the United Kingdom with the Medicines

and Healthcare Products Regulatory Agency (MHRA)

at Market Towers, 1 Nine Elms Lane, London,

SW8 5NQ.

Waters Corporation (Micromass UK Ltd.)

Floats Road

Wythenshawe

Manchester M23 9LZ

United Kingdom

Telephone: +44-161-946-2400

Fax: +44-161-946-2480

Contact: Quality manager

Table of Contents ix

Trademarks ............................................................................................................ ii

Customer comments ............................................................................................ iii

Contacting Waters ............................................................................................... iv

Safety considerations .......................................................................................... iv

Considerations specific to the ACQUITY TUV detector.................................... v

Safety advisories.................................................................................................. v

Operating the ACQUITY TUV detector ........................................................... vi

Applicable symbols ............................................................................................. vi

Audience and purpose......................................................................................... vi

Intended use of the ACQUITY TUV detector.................................................... vi

Calibrating ........................................................................................................ vii

Quality-control .................................................................................................. vii

Authorized representative information ...................................................... viii

Authorized representative............................................................................... viii

ISM classification .............................................................................................. viii

ISM Classification: ISM Group 1 Class B ...................................................... viii

Overview ................................................................................................................ 1

Principles of operation ....................................................................................... 2

Detector optics...................................................................................................... 2

Wavelength verification and test........................................................................ 8

Operational modes............................................................................................... 9

Before you begin ................................................................................................ 12

Installing the detector ...................................................................................... 13

Plumbing the detector ...................................................................................... 15

Installing the multi-detector drip tray ............................................................. 19

Table of Contents

x Table of Contents

Making Ethernet connections ......................................................................... 20

I/O signal connector ........................................................................................... 21

Connecting to the electricity source ............................................................. 21

Starting the detector ......................................................................................... 22

Monitoring detector LEDs................................................................................. 24

About the detector control panel....................................................................... 25

Shutting down the detector ............................................................................. 27

Shutting down for less than 24 hours............................................................... 27

Shutting down for more than 24 hours............................................................. 28

Maintaining the Detector ................................................................................. 29

Contacting Waters technical service................................................................. 29

Maintenance considerations.............................................................................. 30

Proper operating procedures ............................................................................. 30

Maintaining the leak sensor ............................................................................. 32

Replacing the detector’s leak sensor................................................................. 36

Maintaining the flow cell................................................................................... 38

Replacing the lamp ............................................................................................ 49

Replacing the fuses ............................................................................................ 52

Cleaning the instrument’s exterior................................................................... 53

Error Messages ................................................................................................... 54

Startup error messages ..................................................................................... 54

Error messages preventing operation............................................................... 58

Safety Advisories ................................................................................................ 62

Warning symbols ............................................................................................... 62

Caution symbol .................................................................................................. 65

Warnings that apply to all Waters instruments .............................................. 66

Electrical and handling symbols....................................................................... 72

Specifications ...................................................................................................... 74

Solvent Considerations .................................................................................... 77

Introduction........................................................................................................ 77

Solvent miscibility ............................................................................................. 78

Wavelength selection......................................................................................... 81

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