VWR VisiScope 300 Series Manual de usuario

Model European Catalogue Number

TL385FL 630-2724

INSTRUCTION MANUAL

Version: 1

Issued: 14, 12, 2016

VWR

Microscope series 300

Table of Contents

Warning

Safety information

Package Contents

Unpacking

Intended use

Symbols and conventions

Product specications

Overview

Using the microscope

Using the uorescence

Troubleshooting

Repair and maintenance

User replaceable accessories and spare parts

Technical service

Warranty

Compliance with local laws and regulations

Disposal

Legal Address of Manufacturer

Country of origin: ITALY

Europe

VWR International bvba

Researchpark Haasrode 2020

Geldenaaksebaan 464

B-3001 Leuven

+ 32 16 385011

http://be.vwr.com

Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of main-

tenance. It is built to high optical and mechanical standards and to withstand daily use.

We remind you that this manual contains important information on safety and maintenance, and that it must

therefore be made accessible to the instrument users.

We decline any responsibility deriving from incorrect instrument use uses that does not comply with this

manual.

Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off position.

Users should observe all safety regulations of the region. The equipment has acquired the CE safety label.

However, users have full responsibility to use this equipment safely.

Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Package Contents

DESCRIPTION QUANTITY

Microscope stand with nosepiece, stage, condenser 1

Optical head (trinocular) 1

Objective E-PLAN IOS 4x 1

Objective E-PLAN IOS 10x 1

Objective E-PLAN IOS 20x 1

Objective E-PLAN IOS 40x 1

Objective E-PLAN IOS 100x 1

Eyepiece WF10x/20mm 2

Fluorescence attachment 1

Dust cover 1

Power supply output 6Vdc 1

Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the contain-

er and lift the top half of the container. Take care to ensure that the optical items (objectives and eyepieces)

do not fall out and get damaged. Using both hands (one around the neck and one around the base), lift the

microscope from the container and put it on a stable desk.

Place the observation head onto the top of the neck and tighten the lock-screw. Insert the eyepieces into the

eye tubes.

Connect the provided power supply to the power supply input jack on the rear of the microscope.

Intended use

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with

caution

Product specications

Head: Trinocular, 30° inclined, 360° rotating. Interpupillary adjustment 48-75 mm.

Eyepieces: WF10X/20mm.

Nosepiece: 5-position reversed revolving nosepiece. Ball bearing rotation.

Objectives: IOS E-PLAN 4x/0.10, 10x/0.25, 20x/0.40, 40x/0.65, 100x/1.25 (no cover slide).

Focusing system: Coaxial coarse and fine.

Stage: Double layer mechanical sliding stage, dimensions 188x150mm, moving range

78x54mm.

Belt-drive in X direction.

Condenser: Abbe condenser, sliding-in, N.A. 1.25 with centering system.

Illumination: Transmitted light: P-LED3, with manual brightness control.

Epi-fluorescence: high-power white LED (for B ang G filtersets).

Power supply: External power supply: Input 100-240Vac 50-60Hz / Output 6Vdc 1A.

PHOTO/VIDEO PORT

HEAD LOCKING

SCREW

REVOLVING NOSEPIECE

STAGE

TRANSLATION KNOBS

BRIGHTNESS

ADJUSTMENT

(LEFT SIDE)

CONDENSER CENTERING

SCREWS

IRIS DIAPHRAGM

EYEPIECE

CONDENSER

LED ILLUMINATOR

OBJECTIVE

DIOPTRIC

ADJUSTMENT RING

Overview

SLIDE CLAMP

FINE FOCUSING KNOB

CONDENSER HEIGHT

ADJUSTMENT

TENSION

ADJUSTMENT KNOB

COARSE FOCUSING KNOB

FOCUS STOP KNOB

INTERPUPILLARY DISTANCE

Using the microscope

Adjust the observation head

Loosen the lock-screw, turn the observation head to a comfortable position for observation, and then lock the

lock-screw.

Place the specimen on the stage

Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is centred

over the stage opening by adjusting the coaxial knobs of the stage

LED lamp settings – Transmitted illumination

The microscope is tted with high brightness white LED illuminator. Press the selector switch on the back of

the

main body to I position. Turn the brightness adjustment knob to a brightness suitable for observation.

LED lamp settings – Epillumination

The microscope is tted with high brightness LED illuminator. Press the selector switch on the back of the

main body to II position. Turn the brightness adjustment knob to a brightness suitable for observation.

Adjust interpupillary distance

Hold the right and left parts of the observation head by both hands and adjust the interpupillary distance by

turning the two parts until one circle of light can be seen.

Focus and diopter adjustment

Turn the dioptric adjustment ring on the left eyepiece to the zero position. Turn the coarse focus knob in order

to focus the slide with an objective with low magnication. Adjust the ne focus knob until you obtain a clear

and dened picture observing with the right eye, and then act on the left dioptric compensation ring observing

with the left eye. When the image appears in focus, choose the necessary objective with the revolving nose-

piece. Turn the tension-adjust-knob to get a suitable tension for the focus system.

Condenser adjustment

Turn the condenser adjustment knob to move the condenser up or down for a clear and uniform sample il-

lumination. The two condenser centring screws can be used to centre the condenser.

Set the numerical aperture

Adjust the aperture of the iris diaphragm to set the numerical aperture of the illuminator, thus controlling im-

age contrast and resolution.

SLIDE THE BLACK

PLATE BETWEEN

STAGE AND CLAMP

Using the uorescence

Assembling the epi-uorescence attachment

Take the attachment out of its packaging and put it onto the microscope stand, then tighten the attachment lock-

ing screw.

Place the optical head onto the top of the attachment and tighten the optical head locking screw. Connect the

cable from the epi-uorescence attachment to the output jack on the rear of the microscope.

Lighting up the uorescence LED light

Connect the external 6Vdc power supply to a wall socket, and plug its output to the jack on the rear of the

microscope:

In order to light up the epi-uorescence LED, press the main switch on the rear in position II. Then rotate the

brightness adjustment knob on the left to the desired value. Pull the lter selector (1) to right or left in order to

insert the uorescence lter into the optical path. Put the lter selector (1) in the center if you want to use the

brighteld transmitted illumination. Unlike a mercury lamp system illumination doesn’t need any power-up time

for heating, and can be used immediately after switching on. Also, the LED source is pre-aligned in factory and

doesn’t need any alignment operation.

Looking at the uorescent specimen

Focus on your sample, and adjust the light intensity as needed through the brightness adjustment knob. In order

to improve the darkness of the background (thus improving contrast), it is strongly suggested to put the provided

black plate under the stage):

LED ILLUMINATOR

FILTER

SELECTOR (1)

JACK FROM THE

ILLUMINATOR

(UPPER POSITION)

JACK FROM THE

POWER SUPPLY

(LOWER POSITION)

MAIN

SWITCH

Troubleshooting

Review the information in the table below to troubleshoot operating problems.

PROBLEM CAUSE SOLUTION

LIGHT DOESN’T TURN ON Power supply not connected Check that the 6Vdc power supply

jack is well inserted on the rear of the

microscope

Rotate the brightness adjustment con-

trol and check if there is an increase in

the light output

IMAGE CANNOT BE SEEN OR

IS DARK.

The iris diaphragm aperture

is not completely opened

Completely open the iris diaphragm

aperture

Brightness level is low Rotate the brightness adjustment po-

tentiometer

Objective is not aligned with

the optical axis

Rotate the nosepiece until an objective

is well inserted in the optical path (it

“clicks”)

IMAGE IS UNCLEAR, BLURRED

OR HAS INSUFFICIENT CON-

TRAST.

Objectives or lters are dirty Wipe them clean

The iris diaphragm aperture

is not opened correctly

Open the iris diaphragm aperture

completely

Condenser at wrong height Rotate the condenser knob until you

see a uniform illumination

Repair and maintenance

Microscopy environment

This microscope is recommended for use in a clean, dry and shock free environment with a temperature of

0-40°C and a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.

After using the microscope

The microscope should always be kept vertical when moving it so that no moving parts, such as the eye-

pieces, fall out.

Never mishandle or impose unnecessary force on the microscope. Never attempt to service the microscope

yourself. Turn down the illumination intensity control and turn the light off. Cover the microscope with the in-

cluded dust cover, and keep it in a dry and clean place.

Electrical safety precautions

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off-position.

Users should observe all safety regulations of the region. The equipment has acquired the CE safety label.

However, users do have full responsibility to use this equipment safely.

Cleaning the optics

If the optical parts require cleaning rst use compressed air.

If that is not sufcient use a soft lint-free cloth with water and a mild detergent.

And as a nal option use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.

Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or

near electric equipment. Use these chemicals in a well ventilated room. Remember to never wipe the surface

of any optical items with your hands. Fingerprints can damage the optics. Do not disassemble objectives or

eyepieces in an attempt to clean them.

For the best results, use the VWR cleaning kit (see catalogue number below).

If you need to send the microscope to manufacturer for maintenance, please use the packaging if possible.

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