VWR Gel Documentation Imager2 Manual de usuario

Version 01 Issue 04/2021

Instruction Manual

VWR®Gel Documentation Imager2

EUROPEAN CATALOGUE NUMBER: 730-1458

2vwr.com Instruction manual VWR®Imager2

Legal address of Manufacturer

Europe

VWR®International bv,

Researchpark Haasrode 2020

Geldenaak sebaan 464

B-3001 Leuven

+3216385011

http:be.vwr.com

UK Importer:

VWR International Ltd

Hunter Boulevard, Magna Park

Lutterworth, Leicestershire, LE17 4XN

http://uk.vwr.com

Country of origin

3vwr.com Instruction manual VWR®Imager2

Legal Address of Manufacturer

Safety Instructions

Unpacking a Imager2 System

Removing from the Packaging

Visual Inspection

Unpacking the Main Box

Overview

Imager2 Overview

Imager 2

Gel Doc image capture Software

Software Installation

Minimum System Requirements

Software Installation

Run the Set-up Program

Select the Instrument Hardware Information

Select if running Gel Doc in a CFR21 part 11 Compliance environment

Select the Installation Folder Destination Location

Select the Start Menu Folder

Install Device Drivers using the Device Driver Installation Wizard

Operating

Operator Controls

Rear Panel

Touch Screen PC

Connected PC

Instrument Set-up - Standard Darkroom

Operator Accessories

Lights

Filters

Switching Instrument On

Entering Hardware Details

Entering Personal Preferences

Adjusting Basic Screen Viewing Size

Recommended Light / Dye / Accessory and Other Application Tables

Loading Test Samples

Converter Screens Installed

Ultra-slim Blue Light LED Transilluminator Installed

White Light Pad Installed

Automatic Gel Image Capture Mode

DNA/RNA Agarose Gel and DNA/RNA Acrylamide Gel

Visible Protein Gel

Automatic Blot Image Capture

Visible Blot

Automatic Image Capture - Sample Positioning

Automatic Image Capture - Capturing the Image

Automatic Image Capture - Image Actions Following Capture

Manual Image Capture Mode

Manual Image Capture - Start

Manual Image Capture - Single Image

Manual Image Capture - Series

Manual Image Capture - Additive Series

Manual Image Capture - Multiplex

Manual Image Capture - Image Actions Following Capture

Protocols - Saving, Opening and Editing

Introduction

Creating and Saving a Protocol

From the Home Screen

In Auto Image Capture Mode

In Manual Image Capture Mode

Table of Contents

4vwr.com Instruction manual VWR®Imager2

Using Protocols

Opening a Protocol

Deleting a Protocol

Editing a Saved Protocol

Image Functions

Load Images Screen

Image Actions from Load Images Screen

View Images Screen

View Images Screen - Single Images

View Images Screen - Multiplexed Images

View Images Screen - Image Display Options

View Images Screen - Combining Single Images into a New Multiplexed Image

Image Edit Screen

Image Edit Screen - Image Editing Main Options

Image Edit Screen - General Option

Image Edit Screen - Annote Option

Image Edit Screen - Enhance Option

Image Edit Screen - General / Annotate / Enhance Options Common Control

Image Edit Screen - 3D View Option

Image Edit Screen - All Options Common Controls

Common Screen Functions and Tools

Saving Images

Printing

102

Operator Maintenance

109

Looking After a Imager2 Instrument

109

Cleaning a Imager2 Instrument

109

Contacting VWR International

109

User Replacable accessories and spare parts

110

Imager2 Troubleshooting

111

Disposal

112

Disposing of a Imager2

112

The Waste Electrical and Electronic Equipment (WEEE) Directive

112

5vwr.com Instruction manual VWR®Imager2

Safety Instructions

Safety Practices

This document describes the general safety practices and precautions that must be observed when

operating a VWR®Gel Documentation Imager2 System.

This advice is intended to supplement, not supersede, the normal safety codes in the user’s country. The

information provided does not cover every safety procedure that should be followed. Ultimately,

maintenance of a safe laboratory environment is the responsibility of the user and the user’s organisation.

Please consult all documentation supplied with the Imager2 System before starting to work with the

instrument. Carefully read the safety information in this document and in the other documentation supplied.

When setting up the instrument or performing analysis or maintenance procedures, strictly follow the

instructions provided.

Warning Notices

Within this User Guide WARNINGS are used to highlight information or instructions that must be followed

in order to avoid personal injury to yourself or other people in the vicinity, e.g. switch off the mains voltage

and remove the mains cord before cleaning.

WARNINGS appear as below:

WARNING

Switch off the mains voltage and remove the mains cord before cleaning.

WARNING

Ensure that all instrument users read and understand the precautions listed below.

WARNING

You are advised to post a copy of the precautions near to or on the instrument itself.

Precautions

The following precautions must be observed when using a Imager2 System:

Be sure that the voltage of the Imager2 System corresponds to the voltage available where it is to

be installed.

Never remove the side or back panels of the Imager2 System without first shutting down the

instrument and disconnecting the mains cord.

General Operating Conditions

Imager2 Systems have been designed and tested in accordance with the safety requirements of the

International Electrotechnical Commission (IEC). Imager2 Systems conform to IEC61010-1:2010 (Safety

6vwr.com Instruction manual VWR®Imager2

Requirements for electrical equipment for measurement, control and laboratory use) as it applies to IEC

Class 1 (earthed) appliances, and therefore meet the requirements of EC directive 2006/95/EC.

If possible, avoid any adjustment, maintenance or repair to the instrument while it is open and operative.

However, if any adjustment, maintenance or repair is necessary while the instrument is open, this must be

done by a skilled person who is aware of the hazards involved.

Whenever circumstances arise that mean a Imager2 System may be unsafe, make it inoperative. In

particular, a Imager2 System may be unsafe if it:

Shows visible damage.

Fails to perform the intended measurement.

Has been subjected to severe transport stresses.

Has been subjected to prolonged storage in unfavorable conditions.

Environmental Conditions

Imager2 System should only be used under the following conditions:

Indoors.

At altitudes below 2000m.

In ambient temperatures between 5°C and 40°C.

With relative humidity below 80% for temperatures up to 31°C, decreasing linearly to 50% relative

humidity at 40°C.

Electrical supply fluctuations not exceeding +10% of the nominal voltage.

WARNING

The protection provided by the equipment may be impaired if the environmental

conditions do not lie within these parameters.

7vwr.com Instruction manual VWR®Imager2

Electrical Safety

Imager2 Systems are designed to protect the user from potential electrical hazards. This section describes

some recommended electrical safety practices.

WARNING

Lethal voltages are present at certain points within the instrument.

When the instrument is connected to mains power, removing the instrument covers

is likely to expose live parts.

Even when the power switch is off, high voltages can still be present –capacitors

within the instrument may still be charged even if the instrument has been

disconnected from all live voltage sources.

The Imager2 System must be correctly connected to a suitable electrical supply. The supply must have a

correctly installed protective conductor (earth or ground) and must be installed and checked by a qualified

electrician before connecting the instrument.

WARNING

Any interruption of the protective conductor inside or outside the Imager2 System,

or disconnection of the protective conductor terminal is likely to make the

instrument dangerous.

Intentional interruption of the protective conductor is prohibited.

WARNING

Ensure that the mains supply socket on the instrument is not obstructed, i.e. leave a

gap to allow easy disconnection of the mains cord.

WARNING

If the mains power cord has to be replaced, ensure that the replacement cord is

appropriately rated and approved for the intended use.

When working with the Imager2 System:

Connect the instrument to a correctly installed mains power outlet that has a protective conductor

connection.

Do not operate the instrument with any covers or internal parts removed.

Do not attempt to make internal adjustments or replacements except as directed in the Imager2

System documentation.

Disconnect the instrument from all live voltage sources before opening it to make any

adjustments, replacements, maintenance or repair. If the opened instrument must be operated for

further adjustment, maintenance or repair, this must only be done by a supplier’s Service

Engineer.

If it is possible that the instrument is no longer electrically safe for use, make the instrument

inoperative and secure it against any unauthorised or unintentional operation. The electrical

safety of the instrument is likely to be impaired if:

It has any signs of visible damage.

If it has been subjected to prolonged storage in unfavourable conditions.

8vwr.com Instruction manual VWR®Imager2

If it has been subjected to severe stress during transportation.

Changing a Fuse

There are two User accessible fuses that may need to be replaced:

These are located in the mains power socket on the rear panel of the Imager2 instrument and are

only accessible when the mains power cord has been removed.

To change the fuses:

WARNING

Switch off the instrument and unplug the mains power cord from the electrical

supply.

Gently pull out the fuse holder.

Replace the fuses with new fuses of the same type and rating. The fuse type is 20 mm x 5 mm

IEC 60127-1:2006, AMD1:2011 and AMD2:2015 CSV for instruments in all countries.

Replace the fuse holder.

Note: Accessories such as the GelVue Transilluminator may have their own fuse.

Note: If the instrument still does not work correctly after replacing the fuses with the correct

replacements, or if the fuses blow repeatedly, contact the supplier’s Office or Representative.

Electrical Protection

Insulation: Class I rating for external circuits. Only connect equipment that meets the

requirements of IEC 61010-1:2010, IEC 60950-1:2005, AMD1:2009 and AMD2:2013 CSV or

equivalent standards.

Installation Category: The instruments are able to withstand transient overvoltages typically

present on the mains supply. The normal level of transient overvoltages is impulse withstand

(overvoltage) category II of IEC 60664-1:2007.

Pollution Degree 2: Normally only non-conductive pollution occurs. Occasionally, however,

temporary conductivity caused by condensation must be expected.

EMC Compliance

EC Directive

The Imager2 System is designed and tested to meet the requirements of the EC directive 2004/108/EC and

93/68/EEC. The instrument complies with the EMC standard EN61326-1:2013 (EMC standard for electrical

equipment for measurement, control and laboratory use) and EN 55011:2009 and A1:2010 (ISM) class A (rf

emissions).

FCC Rules and Regulations

The Imager2 System is classified as a digital device used exclusively as industrial, commercial or medical

test equipment. It is exempt from the technical standards specified in Part 15 of the FCC Rules and

Regulations based on Section 15.103 (c).

9vwr.com Instruction manual VWR®Imager2

UV Safety

The Imager2 System uses epi mid wave UV tubes and UV transilluminator. If the door is opened the image

capture software will automatically switch off the UV light.

To override the UV light when the door is open for cutting bands out of your gel please follow the

instructions listed below;

1. Open the Imager2 door and locate the top white switch under the black square button. Pull the top

white switch out.

2. Then press the black square button and hold for a few seconds until you hear the UV

transilluminator switch on.

Warning Labels

Warning labels attached to the instrument draw attention to specific hazards - refer to this User Guide and

other documentation provided with the instrument for more details concerning potential hazards and any

precautions or other actions that must be taken.

WARNING

To prevent potential personal injury and damage to the instrument, switch OFF all

components in the system and disconnect them from the mains power supply

before altering, or making any new electrical connections.

WARNING

Please ensure that Personal Protective Equipment (PPE) is used when overriding UV

light source.

It is recommended that a laboratory coat is worn, a UV visor and gloves.

UV override switch

Top white switch needs to be

pulled out to override UV

light.

10 vwr.com Instruction manual VWR®Imager2

Unpacking a VWR®Imager2 System

Removing from the Packaging

Visual Inspection

Upon taking delivery of a new Imager2 Instrument:

Check the contents of the cartons as they are unpacked against the contents listed on the

packing list

Check each item on the packing list for damage and document any damage carefully

If any items are missing or damaged, contact your local distributor immediately

Unpacking the Main Box

The Imager2 Instrument is packaged in a large carton which is securely strapped. Some Instruments may

be shipped as a carton only, others may be shipped with the carton strapped onto a wooden pallet.

Accessories are shipped in one or more separate cartons.

To unpack a typical Imager2 Instrument:

WARNING

A Imager2 Instrument requires two people to lift it safely. A Imager2 Instrument

complete weighs approximately 37kg (Standard model).

UNPACKING A IMAGER2

Original packing of Imager2

Remove the strapping and tape

Open the cardboard carton top flaps

Remove the carton and gently lift the

Imager2 out

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