VWR 462-0353 Manual de usuario

INSTRUCTION MANUAL

VWR 12 and 18 litre shaking water bath

European Catalogue Numbers

462-0353 18 Litre with UK mains plug

462-0354 12 Litre with UK mains plug

462-0355 18 Litre with EURO mains plug

462-0356 12 Litre with EURO mains plug

Version: 1

Issued: 3/1/2013

Legal Address of Manufacturer

Adresse légale du fabricant

Juristische Anschrift des Herstellers

Europe

VWR International bvba

Researchpark Haasrode 2020

Geldenaaksebaan 464

B-3001 Leuven

+ 32 16 385011

http://be.vwr.com

Country of origin

United Kingdom

Pays d'origine

Royaume-Uni

Ursprungsland

Großbritannien/Nordirland

VWR shaking water bath Instruction Manual 31228 V1

European edition Page 1

Instruction manual

Introduction

The VWR shaking water baths are a general purpose series of thermostatically controlled shaking

bath designed for indoor laboratory use by a professional user. Conforms to DIN 12876 Class 1 for

use with non-flammable liquid.

Contents

1 Safety

1.1 Warning symbol

1.2 Safety certification

1.3 Safety features

1.4 Before first operating the equipment

1.5 Precautions during and after operation

2. Getting started

2.1 Unpacking

2.2 Optional accessories

2.3 Electrical supply

2.4 Conditions of use

3. Operation

3.1 Water level

3.2 Flat bottomed vessels

3.3 Operation above 60°C

3.4 Fitting the shaking trolley and tray

3.5 Setting the temperature and shaking speed of baths

4. Specification

4.1 Physical and electrical details

4.2 Performance

5. Maintenance

5.1 Cleaning

5.2 Replacement of fuses

5.3 Routine safety tests

5.4 Service

6. Technical Tips

6.1 Which water should you use in your bath?

6.2 How to prevent rust in water baths

6.3 How to prevent algae and bacteria

7. Troubleshooting

8. Warranty

9. Disposal

31228 V1 VWR shaking water bath Instruction Manual

Page 2 European edition

1. Safety

1.1 Warning symbols

The symbols below are marked on the equipment to indicate:

Caution: Surfaces and water can be hot during and after use.

Read this manual before using the bath

1.2 Safety certification

VWR water baths meet the requirements of international safety standard IEC 61010-2-10 and

IEC 61010-2-51 and national standards based on them including:

UL 61010A-2-010

UL 61010-2-051-04

CAN/CSA-C22.2 NO. 61010-2-010-04;

CAN/CSA-C22.2 No. 61010-2-051

1.3 Safety features

The shaking trolley is driven by a magnetic drive system. The drive magnet in the tank is

magnetically coupled to a motor-driven magnet under the tank. The magnetic coupling

cannot exert enough force to cause injury.

To protect samples in case of failure of the primary temperature control system there is a

second completely independent user-settable control system.

In addition to the second control system for sample protection, there is a separate fixed-

temperature cut-out to protect the bath and surroundings in the very unlikely event of failure

of both control systems.

1.4 Before first operating the equipment

Read the whole of these instructions. Safety may be impaired if they are not followed.

If the equipment has been transported or stored in cold or humid conditions, condensation

may form inside it. If that could have happened, allow time (at least 2 hours) for the

condensation to evaporate before using the equipment.

1.5 Precautions during and after operation

The bath is for use only with water as the bath liquid. Make sure that it cannot become

contaminated by other liquids. The use of de-ionized water is recommended to prevent

corrosion. A full explanation of this is given in Section 6 of this manual.

CAUTION. PLEASE FILL BATH WITH WATER BEFORE SWITCHING ON.

Change the water regularly and empty when not in use for prolonged periods, to further

prevent corrosion and contamination.

Before emptying a bath, allow the water temperature to fall to a safe level. The 18 litre bath,

should be emptied moving.

Do not use the equipment in an area where there are aggressive or explosive chemical

mixtures or atmosphere.

If potentially hazardous liquid is spilt onto the equipment, disconnect it from the power supply

and have it checked by a qualified service technician. It is the user's responsibility to carry

out appropriate decontamination if hazardous material is spilled on the equipment.

Do not use the bath to heat any material that could cause a fire or any other kind of hazard.

VWR shaking water bath Instruction Manual 31228 V1

European edition Page 3

2. Getting started

2.1 Unpacking

Remove packing materials carefully, and retain for future shipment or storage of the

equipment. Standard equipment includes:

Thermostatic shaking bath

Mains cord with plug

Drive magnet

Shaking trolley

Universal tray

Gabled polycarbonate lid

Operating manual

Quick start guide

CAUTION. PLEASE FILL BATH WITH WATER BEFORE SWITCHING ON.

2.2 Optional accessories

VWR European Catalogue number

Universal flask tray - with springs

For 12 litre baths 462-0354, 462-0356

462-0360

For 18 litre baths 462-0353, 462-0355

462-0359

Plain tray

For 12 litre baths 462-0354, 462-0356

462-0358

For 18 litre baths 462-0353, 462-0355

462-0357

Test tube tray –to hold test tube racks

For 12 litre bath, holds 3 test tube racks

462-0361

For 18 litre bath, holds 5 test tube racks

462-0362

Test tube racks

Rack for 10mm tubes

462-0363

Rack for 13mm tubes

462-0364

Rack for 16mm tubes

462-0365

Rack for 19mm tubes

462-0366

Rack for 25mm tubes

462-0367

Rack for 30mm tubes

462-0368

Rack for 0.5mm micro-centrifuge tubes

462-0369

Rack for 1.5mm micro-centrifuge tubes

462-0370

Replacement non drip Polycarbonate Gabled

Lids

For 12 litre baths 462-0354, 462-0356

462-0373

For 18 litre baths 462-0353, 462-0355

462-0374

Stainless Steel Gabled Lids

For 12 litre baths 462-0354, 462-0356

462-0371

For 18 litre baths 462-0353, 462-0355

462-0372

31228 V1 VWR shaking water bath Instruction Manual

Page 4 European edition

2.3 Electrical supply

Check that the supply voltage marked on the serial number plate, and the type of mains

plug, are correct for your mains supply outlet (which must have a ground connector).

To disconnect the equipment from the mains supply, remove the mains plug from the mains

supply outlet. Make sure that the mains plug is easily accessible.

2.4 Conditions of use

The water baths are for indoor laboratory use only. Check that the environmental conditions

of the laboratory are within the following limits:

Temperature

5 to 40 °C

Maximum relative humidity

80 % r.h. in room temperatures up to 31°C

decreasing linearly to 50 % r.h. at 40°C

Altitude

Up to 2,000m above sea level

3. Operation

3.1 Water level

Ensure that the water level is at least 5cm above the bottom of the tank, and not higher than

2.5 cm from the top. This applies both without any vessels in the bath and with the maximum

contents.

If the bath is allowed to run dry, the safety cutout will trip and disconnect the heater. If this

happens, unplug the bath and have the cut-out re-set by a qualified service technician.

Water is the only liquid suitable for use in the baths.

3.2 Operation above 60°C

The supplied gabled lid must always be used above 60°C to optimize the temperature

control; for the bath to operate efficiently and to ensure the set temperature is reached and

maintained.

Without the lid, excessive evaporation will require the bath to be filled more often.

3.3 Use as a thermostatic bath without shaking

If the shaking trolley and drive magnet (see 3.4 below) are lifted out, a shaking bath

can be used as a simple thermostatic bath. When used in this way, if flat bottomed vessels

or objects are to be placed in the bath, always use a raised base tray (optional accessory) to

avoid damage to the under-tank heater.

VWR shaking water bath Instruction Manual 31228 V1

European edition Page 5

3.4 Fitting the shaking trolley and tray

1. Put the drive magnet into the hole in the base of the tank

2. Place the shaking trolley in the tank with the axles parallel to the front panel, and

with the peg of the drive arm in the hole in the top of the drive magnet

3. Place the universal tray on the shaking trolley

3.5 Setting the temperature and shaking speed

3.5.1 Setting the control temperature

1. Turn the knob of the sample protection thermostat to maximum

2. Press control key, the display will show , press control key again. The

display will flash once a second with the current set temperature to show that the

controller is ready to be re-set

3. Press up or down control keys to set the desired temperature

(If no key is pressed for 4 seconds, the display will revert back to showing

the actual temperature and the set point will remain at its original value)

4. Press control key. This will store the requested value and the display will

revert to showing the actual water temperature. The water temperature will

change to the new set value

5. When the temperature has stabilized, measure the temperature with a

thermometer. If it is different from the set point temperature use the single point

calibration method (section 3.5.3) to adjust the display temperature

31228 V1 VWR shaking water bath Instruction Manual

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3.5.2 Setting the sample protection thermostat (if required)

1. Turn the knob of the sample protection thermostat to maximum

2. Set the control temperature 2°C above the desired operating temperature, and

wait for the temperature to stabilize

3. Turn the knob of the sample protection thermostat slowly anti-clockwise until a

click is heard and is displayed

The audible alarm will sound continuously to warn you that the bath is controlling

using the sample protection thermostat.

4. Re-set the control temperature to the desired temperature

The audible alarm will sound continuously until the primary control system is re-

activated.

In the unlikely event of failure of the primary temperature control system, the sample

protection thermostat will maintain the water in the bath at a temperature a few degrees

above the set temperature and with greater fluctuations. The display will cycle between 

and the actual bath temperature. If this happens, first check that the sample protection

thermostat is not set to a lower temperature than the primary control system. If that is not the

reason, the primary control system is not functioning correctly. The bath can continue to be

used without compromising the safety of persons or the surroundings until current work is

completed. However, it is recommended to have the bath checked by a qualified service

technician as soon as conveniently possible.



3.5.3 Setting the shaking speed

1. Press control key, the display will flash 

2. Press up control key until the display changes to a moving symbol:

3. Press control key. The display will show the present shaking speed

4. Press up or down control keys to select the required speed

(off, or between 40 and 400)

5. Press control key to set the speed to the value displayed

(If no key is pressed for 4 seconds, the speed will revert to the previous

value)

Sample protection thermostat

Control keys

VWR shaking water bath Instruction Manual 31228 V1

European edition Page 7

The maximum speed for a particular application will depend on number and size of vessels,

and the depth of the water. The shaking trolley is driven by a drive magnet under the tank,

magnetically coupled to the trolley through the tank. As the speed is increased a point is

reached where the magnetic coupling de-latches, causing the trolley either to move erratically

or to stop.

The shaking speed will revert to previously set value when reconnected after power

interruption.

3.5.4 Single point calibration (SPC)

The display of set temperature and actual temperature is accurate to within 1°C at 37°C but

may be up to 5° C out at maximum temperature. Using SPC the display can be re-set to be

accurate to ±0.1° C at a single point by the following procedure:

1. Set the bath to the calibation temperature and allow the bath temperature to

stabilze for 20 minutes and indicated by the display not fluctuating by more than

0.2°C. Measure the actual water temperature with a calibrated thermometer

2. Press control key. The display will show ,press the up control key

until the display changes to show , press control key to enter SPC mode.

The display will flash once a second with the current value and shows that the

controller is ready to be re-set

3. Press up or down control keys to set the amount of correction value

(positive or negative) as measured by a thermometer. (If no key is pressed for 4

seconds, the display will revert back to showing the actual temperature and the

set point will remain at its original value)

4. Press control key. This will store the new corrected set temperature and the

display will revert to showing the new correct actual water temperature

4 Specification

4.1 Physical and electrical details

Mains supply 230V @ 50/60 Hz

Pollution degree: 2

Installation Category: II

Note: Mains supply voltage fluctuations are not to exceed ±10% of the nominal supply voltage

Models

Capacity/Litres

Current Rating/Amps

462-0354

462-0356

12L

2.8A

462-0353

462-0355

18L

5.4A

4.2 Performance

Range

5°C above ambient to 99°C

Display (also used for setting)

10.0 to 99.0°C in steps of 0.1°C

Temperature stability to DIN 12876-3 *

± 0.2°C

Shaking speed range

40 to 400 strokes per minute

The maximum shaking speed for a particular application will depend on the number

and size of vessels in the shaking tray, and the depth of the water).

* The supplied polycarbonate lid must be used to achieve the quoted performance

31228 V1 VWR shaking water bath Instruction Manual

Page 8 European edition

5 Maintenance and service

No routine maintenance is required except for cleaning.

5.1 Cleaning

Clean the equipment with a damp cloth, using water only. Do not use chemical cleaning

agents. Before using any other cleaning or decontamination method, check with the

manufacturer or supplier to make sure that the proposed method will not damage the

equipment.

Scale on immersed parts can be removed using chemical de-scaling products designed for

use on kitchen equipment which has metal heating elements.

Warning - De-scaling products may be toxic - follow the manufacturer's instructions

5.2 Replacement of fuses

Fuses are Littlefuse 3AB 314 series. Fast-acting, high breaking current (max breaking

current at least 750 A). Dimensions; 1.25 inch long, 0.25 inch diameter. Replace fuses only

by the same type and rating (250volt).

Models

Capacity

Fuse rating/ Amps

462-0354

462-0356

12L

462-0353

462-0355

18L

Replace fuses as follows:

1. Disconnect the unit from the power supply

2. Remove the mains input connector from the socket at the back of the bath

3. Press down the fuse drawer catch

4. Pull out the fuse drawer, check the fuse(s) and replace it if necessary, using

the fuse type and rating specified above

5. Push back the drawer, and replace the mains input connector

5.3 Routine safety tests

If routine tests are to be made, we recommend a test of the integrity of the protective earth

conductor and an insulation test at 500 Vd.c. Routine flash tests are not recommended for

any electrical equipment, because repeated high voltage tests degrade insulation materials.

5.4 Service

Web Resources

Visit the VWR’s website at www.vwr.com for:

•Complete technical service contact information

•Access to VWR’s Online Catalogue, and information about accessories and related products

•Additional product information and special offers

visit. www.vwr.com.

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