THORLABS OTKB Manual de usuario

Optical Trap Kit

Manual

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 2 www.thorlabs.com

Table of Contents

1. Safety ............................................................................................................................................3

2. Introduction..................................................................................................................................4

3. Setup Description and Segments.................................................................................................4

4. Optical Trap Kit Components......................................................................................................8

5. Force Measurement Module OTKBFM....................................................................................11

6. Setup and Alignment in Detail ..................................................................................................12

7. Software Package.......................................................................................................................24

8. Sample preparation and measurement .....................................................................................24

9. Accessories .................................................................................................................................25

10. Summary of Key Features and Specifications..........................................................................26

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 3 www.thorlabs.com

Part 1. Safety

All statements regarding safety of operation and technical data in this instruction manual will only apply

when the unit is operated correctly.

WARNING

This unit must not be operated in explosive environments

WARNING

Make sure the fuse setting at the back of the laser diode driver and temperature controller

are appropriate for your region. Failure to do this will result in damage to the controllers.

Set TEC and max LD current parameters according to the specifications of the trapping

laser diode before operating the Optical Trapping Kit. Failure to do so may result in damage

of trapping laser diode. Also refer to the manual for the white light source current driver.

Failure to do this may result in damage to the LED.

WARNING

Latex gloves should be worn to prevent oil from fingers from reaching all optical surfaces.

Make sure you use appropriate laser safety glasses during operation.

WARNING

Avoid Exposure –ASE and laser radiation emitted from apertures.

Never look directly in to beam.

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 4 www.thorlabs.com

Part 2. Introduction

In 2007, three researchers in the Department of Biological Engineering at MIT –Steve Wasserman,

David Appleyard, and Matthew Lang –built an optical trapping setup for use in teaching labs. Their

results were published in the American Journal of Physics [S. Wasserman, D. Appleyard, and M. Lang,

Optical Trapping for Undergraduates, Am. J. Phys. 75, (2007)]. Based on their design, Thorlabs has

collaborated with the aforementioned authors to design an OTKB optical trapping kit that includes all

necessary components and provides the same capabilities. Moreover, since Thorlabs‟ components are

designed to be compatible with each other, the OTKB optical trapping kit is easily modified to provide

additional functionalities as your research needs evolve. For example, adding the ability to steer / scan

the optical trap position. Alternatively, an excitation light source can be easily added by incorporating a

beam splitting cube into the beam path allowing e.g fluorescence measurements. This simple and

modular nature of the system allows you to adapt it for a wide variety of applications.

All of the components needed to construct a fully functional optical trapping system are included in this

cost effective kit (OTKB contains imperial components while OTKB/M contains their metric

counterparts). The system ships in five pre-assembled segments making it very easy to build your optical

tweezers system in a short time.

Part 3. System Description and Segments

The OTKB(/M) system is shipped in five pre-assembled segments, which makes it very easy to build

your optical tweezers system in a relatively short time. There is the collimator segment, the beam

expander segment, the vertical segment, the stage assembly and the camera segment. All that is needed

is mostly coupling these segments together and final laser alignment. These four segments will be

described below, including photos.

3.1 The Collimator Segment:

This includes mainly the fiberport which has been pre-adjusted for collimation at 975nm wavelength.

First check the collimation and alignment by referring to Section 6.1, steps 3) and 4) and if more

adjustments need to be done, follow step 5) too. Once you are satisfied with the collimation and

alignment to the center, this segment connects to the four ER1 rods on the KCB1 of the Beam Expander

Segment.

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 5 www.thorlabs.com

Figure 5.1: The Collimator Segment

3.2 The Beam Expander Segment:

This includes the beam expansion achromatic doublets and the cube assembly (with the dichroic) for

reflecting the trap laser and transmitting the visible spectrum for camera viewing. It also includes the

imaging lens for the camera. The Collimator segment, the Camera segment and the Vertical segment all

connect to this at its three ports as shown in Figure 5.2. Remember to screw the CPB1 to the table as

indicated in picture. After connecting the Collimator segment, check the collimation and alignment of

the expanded beam as described in Section 6.2, steps 14) to 16). Align the dichroic as described in same

section, steps 21) to 22).

Figure 5.2: The Beam Expander Segment

Remove caps to

connect Fiber

To KCB1 on Beam

expander Segment

The Camera

Segment Port

The Collimator

Segment Port

The Vertical

Segment Port

Screw to table

Imaging Lens

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 6 www.thorlabs.com

3.3 The Vertical Segment

This segment holds the objective, condenser and light source as could be seen in Figure 5.3. To mount

this segment, first remove the top part of whole assembly by unlocking the two screws that connect this

to the vertical post. Next, release the screw attaching the lower C1500 to the vertical post and adjust its

height until the four ER1.5 rods can slide into the four cage rod holes on the fixed C6W of the Beam

expander segment. Lock the C1500 to the DP14 once the height is right. Slide the four ER1.5 rods (with

vertical post base sliding also) into C6W just enough such that the rods don‟t interfere with the rotation

of the B4C in the cube. Use the four set screws on the sides of the C6W to hold rods tightly to the cube.

The DP14 Post can now be locked down to the table (using CL5 clamps) along with the CPB1 bases

(using CL6 clamps) when the holes in the base do not align. Then follow the alignment instructions as

described in Section 6.3 steps 6) to 13).

Next, mount the top part that was initially removed and to align this correctly, follow the instructions in

Section 6.5, steps 16) to 21)

Figure 5.3: The Beam Expander Segment

Top Part. Remove

initially for

alignment

C1500 that

attaches lower

Part to DP14

Connects to C6W

here

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 7 www.thorlabs.com

3.4 The Stage Assembly

The stage assembly parts ship in their separate boxes and will have to be assembled as described in

Section 6.8.

3.5 The Camera Segment

This segment mainly has the CCD camera for live imaging. The imaging lens is already connected to the

camera port on the cube, C6W. To complete this segment, just slide the four ER1 rods into the four cage

rod holes on the C6W, such that it doesn‟t interfere with the B4C. Then lock this segment to the C6W

using the four set screws on the sides of the C6W.

Figure 5.4: The Beam Expander Segment

Connects to C6W

here

Cage plate gasket,

CPG3

Camera

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 8 www.thorlabs.com

Part 4. Optical Trapping Kit OTKB(OTKB/M) Components

This section provides some technical details on the individual components used to build this system.

Like many optical trapping systems, this one is based on an inverted

microscope design. The structure of the inverted light microscope is

constructed using Thorlabs‟ 60mm cage system, which is supported by a

damped 1.5″ post (Item # DP14). All of the parts discussed in this

section are included in the OTKB kit unless otherwise noted. Some of the

parts mentioned below are not universal so the metric version of the part

is used in the OTKB/M kit.

1) The 975 nm trapping laser source is a pigtailed Fiber Bragg

Grating (FBG) stabilized single mode laser diode in a

hermetically sealed 14-pin butterfly package. The integrated TEC

element and thermistor in the butterfly package allow the

temperature of the laser to be precisely controlled when mounted

in the LM14S2 laser diode mount and controlled using an

LDC210C laser diode current driver and a TED200C temperature

controller. This laser, mount, and controller combination was chosen to ensure that the output

power (330 mW max) of the laser will be extremely stable, which is important to maintaining a

constant trapping force.

2) The FiberPort (Item # PAF-X-7-B) collimates the output of the trapping laser. The FiberPort is a

versatile collimator since it allows the aspheric collimation lens to be precisely positioned with 5

degrees of freedom (X, Y, Z, Pitch, and Yaw). For polarization sensitive applications, the

keyway on the FiberPort can be rotated about the optical axis so that the orientation of a linearly

polarized collimated beam can be set.

3) The two achromatic doublet lens pairs ACN254-050-B and AC254-150-B expand the collimated

trapping laser beam by a factor of 3.

4) The dichroic mirror that is mounted in the central C6W reflects 980 nm light (trapping source)

while passing visible light.

5) Visible light from the LED light source illuminates the sample and is then imaged on the 1280 x

1024 pixel color CCD camera (Item # DCU224C) using an achromatic doublet AC254-200-A.

The dichroic mirror in the light path in combination with a short pass filter prevents

backscattered light from the 980 nm laser from saturating the CCD detector.

6) A 100X oil immersion Nikon objective lens (Item # OTKOBJ) is used to focus the 980 nm laser

beam down to form the optical trap. The calculated diffraction limited trap diameter is 1.1µm. A

10X Nikon objective is used as the condenser (Item # OTKCON) and collimates the beam after

the optical trap.

Figure 3.1: Pigtail laser, mount

and controllers

LM14S2

LDC210C

TED200C

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 9 www.thorlabs.com

Figure 3.2: Model of Thorlabs Optical Trapping

system

Optical Trap Kit

19590-D04-V15; Apr 12, 2012 Page 10 www.thorlabs.com

7) The sample stage consists of a microscope slide holder mounted to a 3-axis (X, Y, and Z)

translation stage that is mounted on a 1-axis long travel translation stage and to a translating

breadboard which results in the following capabilities:

A) 2" (50 mm) and 2.4" (60mm) of travel perpendicular to the beam path. This makes it easy

to load the sample and coarsely position it near the

trap. The mounting configuration shown can easily

be modified to suit other desired translations.

B) 4 mm of travel in the X, Y, and Z directions using the

modular nanomax differential micrometer drive with

coarse knobs providing 0.5 mm/rev.

C) 300 µm of travel in the X, Y, and Z directions using

the differential knobs (50 µm/rev) on the 3-axis

differential drives.

D) 20 µm of travel in the X, Y, and Z directions using

the piezo actuators (20 nm resolution without using

feedback from the internal strain gauge sensors, 5 nm

resolution using the internal strain gauges for positional feedback) on the 3-axis stage.

Three T-Cube Piezo Drivers (Item # TPZ001) are included in the kit. (Two T-Cube Strain

Gauge (Item # TSG001) are included with the OTKBFM force module.)

8) The visible light emitted from the LED passes through the dichroic mirror and illuminates the

sample while the 980 nm laser beam is reflected towards the optional OTKBFM Force

Calibration Module. (If the OTKBFM is not being used, the laser is blocked by a cap, SM1CP2).

9) A single emitter white light LED (Item # LEDWE-15,7.5ohalf angle forward radiating) is built

into the component OTKB-LS-SP. The light from the LED illuminates the sample.

10)The OTKBFM force measurement module contains the hardware needed to calibrate the trap.

The iris at the back focal plane of the objective (used as the condenser) is imaged on the

PDQ80A Quadrant Position Detector (QPD) using a 40 mm focal length biconvex BK7 lens

(Item # LB1027-B). The detector is silicon based segmented quadrant position-sensing detector

with a rise time of 40 ns and a bandwidth of 150 kHz. The signal generated by the QPD is

sensitive to the relative displacement of the trapped particle from the laser beam axis. As a result

the output of the detector can be used to calibrate the position, stiffness, and force of the optical

trap. A TQD001 T-Cube Quadrant Detector Reader is included with this optional force module.

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