ThermoFisher Scientific QuantStudio 3 Guía de inicio rápido

For Research Use Only. Not for use in diagnostic procedures.

QuantStudio™3 and 5 Real-Time PCR Systems

INSTALLATION, USE, AND MAINTENANCE

Firmware v1.2.x

Publication Number MAN0010407

Revision C.0

The information in this guide is subject to change without notice.

DISCLAIMER

TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT,

PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Limited Use Label License No. 474: Real-Time PCR System for Research Use Only

Notice to Purchaser: The purchase of this instrument conveys to the purchaser the limited, non-transferable right to use the purchased instrument

only, under intellectual property rights that are owned and/or controlled by Life Technologies and relate speciﬁcally to the instrument. Purchase of the

instrument includes the right to use the instrument for internal research and to perform services (including the right to report the results of services

for a fee) by the purchaser only, but does not convey rights to use any other products, reagents, assays or methods such as the 5' nuclease assay

process. The sale of this instrument is expressly conditioned on the purchaser not reselling, repackaging, or distributing this instrument, or any of its

components, and no such rights are conveyed expressly, by implication, or by estoppel. For information on obtaining additional rights, please contact

outlicensing@lifetech.com or Out Licensing, Life Technologies, 5791 Van Allen Way, Carlsbad, California 92008.

Limited Use Label License No. 572: Cy®and CyDye®

Notice to Purchaser: Cy®and CyDye®are registered trademarks of GE Healthcare.

Corporate entity

Life Technologies | Carlsbad, CA 92008 USA | Toll Free in USA 1.800.955.6288

TRADEMARKS

Cy is a trademark of GE Healthcare UK Limited. Adobe, Acrobat, and Reader are trademarks of Adobe Systems, Inc. Microsoft, Internet Explorer, and

Windows are trademarks of Microsoft Corporation. Apple, Safari, and Macintosh are trademarks of Apple, Inc. Mozilla and Firefox are trademarks of

Mozilla Foundation. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. All trademarks are the

property of Thermo Fisher Scientiﬁc and its subsidiaries unless otherwise speciﬁed.

Contents

About this guide ............................................................ 8

Revision history ................................................................. 8

Purpose ....................................................................... 9

■CHAPTER 1 Product information ...................................... 10

Instrument hardware description ................................................ 10

Instrument overview ....................................................... 10

Parts of the instrument ..................................................... 13

Software description ........................................................... 14

Instrument, desktop, and cloud software features ............................. 14

Folders, templates, experiments, and projects ................................ 15

Third-party software installation ............................................ 16

Network connection options ..................................................... 16

Experiment types .............................................................. 17

■CHAPTER 2 Start, sign on, and conﬁgure the instrument .......... 19

Installation and instrument veriﬁcation ........................................... 19

Precautions for use ............................................................ 20

Power on the instrument ........................................................ 20

Parts of the home screen ....................................................... 21

Use the instrument without signing in ............................................ 23

Create a new instrument proﬁle ................................................. 23

Sign out ....................................................................... 24

Link your instrument proﬁle to your Thermo Fisher Cloud account ................... 24

Link your instrument proﬁle to Thermo Fisher Cloud ........................... 24

Create an instrument proﬁle and link to Thermo Fisher Cloud ................... 24

Conﬁgure instrument settings ................................................... 25

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

■CHAPTER 3 Create and run experiments on the instrument ....... 28

Workﬂow ..................................................................... 28

Options for running an experiment ............................................... 29

Create and run an experiment from a template ................................ 29

Run an experiment from a saved ﬁle ......................................... 29

Repeat your last experiment run ............................................. 30

Edit an experiment before starting a run .......................................... 31

Deﬁne experiment properties ............................................... 31

Edit a method ............................................................. 32

Deﬁne plate wells .......................................................... 35

Load and unload a plate in the instrument ......................................... 36

View, pause, or stop a run ....................................................... 37

View well details ........................................................... 37

Pause or stop a run ........................................................ 37

Adjust the graphical view of an experiment ................................... 37

Lock the touchscreen during a run ........................................... 38

Transfer, view, or manage ﬁles and results ........................................ 38

Transfer experiment results ................................................ 38

View run history ........................................................... 38

Manage template (.edt) ﬁles ................................................. 39

■CHAPTER 4 Calibrate and verify instrument performance ......... 40

Calibration and veriﬁcation schedule ............................................. 40

Calibration descriptions ........................................................ 41

View the calibration status and set reminders ..................................... 41

View calibration status and set reminders in the instrument .................... 41

View calibration status and set reminders in the Cloud ......................... 42

Perform ROI/Uniformity, Background, and Dye calibrations .......................... 42

Workﬂow: Calibration ...................................................... 42

Prepare a calibration plate .................................................. 43

Perform calibrations ....................................................... 44

View calibration images and transfer results to USB ........................... 46

Troubleshoot calibration failure ............................................. 47

Identify contamination ...................................................... 47

Create a background plate

(optional)

......................................... 48

Perform instrument veriﬁcation using RNase P plates .............................. 49

Instrument veriﬁcation description and schedule .............................. 49

RNase P Instrument Veriﬁcation Plates ....................................... 49

Analytical performance ..................................................... 50

Installation speciﬁcation .................................................... 50

Prepare an RNase P plate ................................................... 50

Perform RNase P veriﬁcation ............................................... 51

Troubleshoot veriﬁcation failure ............................................. 53

Contents

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

Calibrate custom dyes .......................................................... 54

Custom dyes overview ...................................................... 54

Dilute the custom dye to an optimal concentration ............................. 54

Calibrate the custom dye ................................................... 57

Calibrate for a custom melt curve experiment ..................................... 60

■CHAPTER 5 Maintain the instrument ................................. 62

Backup or restore the instrument ................................................ 62

Decontaminate the sample block ................................................. 63

Materials required ......................................................... 63

Clean the sample block ..................................................... 63

Solvents for cleaning the sample block ....................................... 65

Replace the instrument fuses .................................................... 65

Materials required ......................................................... 65

Replace the fuses .......................................................... 66

Power on or off, store, and move ................................................. 66

Enable sleep mode ......................................................... 66

Power on the instrument ................................................... 67

Power off the instrument ................................................... 67

Prepare the instrument to ship, move, or store ................................ 67

Move the instrument ....................................................... 68

Return the instrument for service ............................................ 68

■CHAPTER 6 Proﬁle and instrument conﬁguration tasks ............ 70

Initial start up ................................................................. 70

Manage proﬁles ............................................................... 71

Create an administrator proﬁle .............................................. 71

View all user proﬁles (Administrator only) .................................... 71

Manage all instrument proﬁles (Administrator only) ........................... 71

Edit a user proﬁle .......................................................... 72

Enable SAE mode (Administrator only) ............................................ 72

Require sign-in (Administrator only) .............................................. 73

Enable remote instrument monitoring (Administrator only) .......................... 73

Update instrument software (Administrator only) .................................. 73

Restore factory defaults ........................................................ 74

Conﬁgure the network .......................................................... 74

Set up a wired connection ................................................... 74

Set up a wireless connection ................................................ 75

Select a Cloud region (Administrator only) ......................................... 75

Manage the instrument name (Administrator only) ................................. 75

Set the date and time ........................................................... 76

Manage the Sign Out Timer (Administrator only) ................................... 76

Contents

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

■APPENDIX A Install and connect the instrument to a network ..... 77

Workﬂow: Install and connect to a network ........................................ 77

Before you begin ............................................................... 78

Unpack and install the instrument ............................................... 78

Power on and follow the startup wizard ........................................... 79

Connect the instrument and the computer directly or to a LAN ....................... 79

Instrument and computer connections ............................................ 81

Download and install the QuantStudio™Design and Analysis desktop Software ......... 82

Computer requirements for the desktop software .............................. 82

Download the desktop software ............................................. 82

Install the software ........................................................ 82

Networking ................................................................... 84

Supported network conﬁguration options ..................................... 84

Control and monitor networked instruments .................................. 85

About the Ethernet port .................................................... 85

Firewall ports that must be open ............................................ 85

Networking guidelines and best practices ..................................... 85

■APPENDIX B Troubleshooting ......................................... 87

■APPENDIX C Parts and materials ..................................... 88

Kits, consumables, and accessories .............................................. 88

96-Well (0.2-mL) consumables .................................................. 88

384-well consumables .......................................................... 89

96-Well Fast (0.1-mL) consumables .............................................. 90

QuantStudio™3 and 5 Systems accessories ........................................ 91

General-use materials and consumables ......................................... 91

■APPENDIX D Instrument speciﬁcation and layout ................... 92

Conﬁgured system dimensions .................................................. 93

Instrument clearances ..................................................... 94

Electrical requirements ......................................................... 94

Environmental requirements .................................................... 95

Network requirements .......................................................... 95

Contents

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

■APPENDIX E Safety ..................................................... 97

Symbols on this instrument ..................................................... 98

Conformity symbols ........................................................ 99

Safety alerts on this instrument ................................................. 100

Location of safety labels on the instrument .................................. 100

Safety information for instruments not manufactured by Thermo Fisher Scientiﬁc .... 101

Instrument safety ............................................................. 101

General ................................................................. 101

Physical injury ........................................................... 102

Electrical ................................................................ 103

Cleaning and decontamination ............................................. 103

Safety and electromagnetic compatibility (EMC) standards ......................... 103

Safety compliance ........................................................ 104

EMC .................................................................... 104

Environmental design ..................................................... 105

Chemical safety ............................................................... 105

Biological hazard safety ........................................................ 106

■Documentation and support ........................................... 107

Related documentation ........................................................ 107

Obtain information from the Help system ........................................ 108

Customer and technical support ................................................ 108

Limited product warranty ...................................................... 109

Index ..................................................................... 110

Contents

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

About this guide

Revision history

Revision Date Description

C.0 December 2015 Updates – describes new features in Firmware

v1.2.x, including:

• Updated workﬂow to create an instrument

proﬁle

• Streamlined workﬂow for connectivity to the

Thermo Fisher Cloud

• Improved display of VeriFlex™Zones

• Plate insert reminder before starting run

• Support for 96-well Fast (0.1 mL) plates

B.0 September 2015 Updates – describes new features in Firmware

v1.1.x, including:

•Experiment runs: monitor real-time data, edit

cycle number and lock screen during a run, view

end plot, and support for 384-well plates

•File management: access Network folders,

navigate folder structures and save templates

on instrument, and perform batch actions for

ﬁle management

•Instrument conﬁguration: support for Security,

Audit, and e-Signature (SAE), smart monitoring,

and choice of server region

Includes information on software feature

comparison, deﬁnitions of terms, parts of method,

network connection options, experiment types,

desktop software installation.

A.0 April 2015 New document. Describes installation, operation,

and maintenance of the QuantStudio™3 and 5 Real-

Time PCR Systems.

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

Purpose

This guide provides information about installing, using, and maintaining the

QuantStudio™ 3 and 5 Real-Time PCR Systems.

Note: For information and instructions on performing experiments on these systems,

refer to the QuantStudio™ Design and Analysis desktop Software User Guide (Pub. no.

MAN0010408).

About this guide

Purpose

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

Product information

■Instrument hardware description ....................................... 10

■Software description .................................................. 14

■Network connection options ........................................... 16

■Experiment types ..................................................... 17

Instrument hardware description

The QuantStudio™ 3 and 5 Real-Time PCR Systems use uorescent-based polymerase

chain reaction (PCR) reagents to perform:

•Quantitative detection of target nucleic acid sequences (targets).

• Qualitative detection of targets (endpoint analysis).

• Qualitative analysis of the PCR product (post-PCR melt curve analysis).

The following xed-block congurations are available:

QuantStudio™3 Real-Time PCR System QuantStudio™5 Real-Time PCR System

• 96-Well VeriFlex™0.2-mL Block

(4 Color)

• 96-Well VeriFlex™0.1-mL Block

(4 Color)

• 96-Well VeriFlex™0.2-mL Block

(6 Color De‑coupled)

• 384-Well Block (5 Color)

• 96–Well VeriFlex™0.1-mL Block

(6 Color De‑coupled)

The instrument can be run directly from the touchscreen to create and start

experiments. To design experiments or to analyze data, the instrument can be

integrated with QuantStudio™ Design and Analysis Software (desktop or cloud).

An optional barcode scanner and optional wireless adapter can be purchased

separately.

Instrument

overview

QuantStudio™3 and 5 Real-Time PCR Systems Installation, Use, and Maintenance Guide

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