Thermo Scientific EVOS M5000 Manual de usuario

For Research Use Only. Not for use in diagnostic procedures.

EVOS™M5000 Imaging System

Pub. No. MAN0017765 Doc. Part No. 710209 Rev. B.0

This document is intended as a benchtop reference for the users of the EVOS™M5000 Imaging System (Cat. No. AMF5000).

For detailed instructions and troubleshooting information, refer to the EVOS™M5000 Imaging System User Guide (Pub. No.

MAN0017563). For installation instructions, refer to the EVOS™M5000 Imaging System Installation Guide (Pub. No.

MAN0017783). These documents are provided on the USB flash drive and are also available for download at

thermofisher.com.

Note: For safety and biohazard guidelines, see the “Safety” appendix in the EVOS™M5000 Imaging System User Guide

(Pub. No. MAN0017563). Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate

protective eyewear, clothing, and gloves.

Graphical user interface (GUI).......................................................................................... 1

Capture tab ...................................................................................................................... 2

Review tab ....................................................................................................................... 7

Settings ............................................................................................................................ 8

Instrument exterior components and mechanical controls.............................................. 10

Technical specifications.................................................................................................. 11

Related documentation and support .............................................................................. 12

Graphical user interface (GUI)

GUI layout

The GUI of the system consists of the Viewing area on the left and function tabs and buttons on the

right. Each tab and button opens the controls necessary to execute the selected function.

Cloud for image storage and

analysis.

Viewing area: Displays the

sample.

Capture tab: Contains the

controls for image capture.

Review tab: Allows you to

review and annotate

captured images.

Keyboard button: Opens the

virtual keyboard.

Settings button: Opens the

Settings tab, which allows

you to select and adjust

basic and advanced system

settings.

EVOS™M5000 Imaging System Quick Reference Guide

Capture tab

Allows you to sign in to and sign out of your Thermo Fisher Cloud account. You can

choose to save your captured images in your Cloud account for image storage (in addition to local

storage) and subsequent analysis with the EVOS™Image Analysis application.

Viewing area: Displays the sample.

Zoom slider:Zooms in and out of the Viewing area. The zoom range is

100% to 1000%.

Active objective and phase ring: Displays

the active objective and phase ring

information.

Channel:

Selects the light source from the

installed LED light cubes (fluorescent channels) or

from the condenser (transmitted light).

RGB Trans option outputs a color image from

interlaced RGB images in the Live mode.

•Select a channel to turn on the excitation light and enter the instrument in the Live

mode. In the Live mode, you can use the brightness and focus controls.

•Click the selected Channel button again to turn off the excitation light and exit the Live

mode. The current channel remains selected as indicated by the blue line underneath

the channel button.

Brightness mode: Toggles between Simple and Actual modes for brightness controls.

•Simple mode allows you to control light intensity as a single Light parameter.

•Actual mode allows you to adjust Brightness (i.e., LED intensity), Exposure, and Gain

parameters

individually.

EVOS™M5000 Imaging System Quick Reference Guide

Brightness controls: Adjust the brightness for the selected channel.

Simple

Actual

Current Z (µm):

Displays the current focus position in µm along the Z-axis.

To change the Z-axis focus position, enter the desired value.

Autofocus (Coarse and Fine): Runs the coarse and fine autofocus

algorithms in the selected channel.

Coarse and Fine focus sliders:

Allow you to adjust the focus in the selected

channel when in the Live mode.

Using the Autofocus Maximum and Autofocus Minimum controls (blue

triangles on the Coarse focus slider), you can limit the autofocus algorithm

to the selected region along the Z-axis.

Saved Settings: Allows you to save and edit Z-axis focus positions

and brightness settings for future use.

Press the button to save the current settings.

Lock Z: Allows you to lock the Z-axis offsets, which specify the optimal focus position in

each channel relative to the focus position in other channels.

•When locked, movement of the Z-position in one channel changes the Z-position in all channels,

preserving their relative Z-positions.

•When unlocked, adjusting the Z-position only affects the currently selected channel.

Enable focus knob: Enables the manual focus knobs.

Capture:

Captures an image using the current capture settings and stores it in the

image cache of the channel in which it was captured.

You can capture multiple channels by selecting the

corresponding channel checkboxes.

Thumbnails: Display the most recently captured

image stored in the memory buffer for the

channel.

EVOS™M5000 Imaging System Quick Reference Guide

Display settings and analysis tools: Allow you to

change image display settings for the Viewing area,

and analyze and annotate captured images.

To open a tool, click the corresponding button. Click the button again to close it.

Image Display Settings: Opens the Image Display Settings tool, which allows you to adjust

image display parameters (Brightness , Constrast , Gamma Correction ) for the

selected channels.

Grid/Grid Settings: Display Grid button switches the display of

the grid in the Viewing area on and off.

Grid Settings allows you to select the grid size.

Scale Bar/Scale Bar Settings: Display Scale Bar button switches the

display of the scale bar in the Viewing area on and off.

Scale Bar Settings allows you to select scale bar color and to display or

hide end bars.

Histogram: Opens the Intensity

Histogram window, which displays

the Pixel count vs. Intensity plot,

where Intensity is the number of

photons detected by the camera

sensor.

Measurements and Annotations:

Allows you to draw regions of interest

(rectangle, ellipse, polygon, line, or

free-form) on the captured image and

measure dimensions, area, or

perimeter of the drawn region.

Show Cell Count: Allows you to perform cell counts using the Auto Count or Manual Count

tools.

•Auto Count: Allows you define auto count parameters by selecting representative target

objects, then count the objects by intensity, area, and circularity.

•Manual Count: Allows you to manually mark items onscreen using up to six separate

labels and keep a running tally of the counts with percentages for each label.

•Confluence: Allows you to select up to 5 reference objects each for target (i.e., cells) and

background in your image to automatically calculate the percentage confluence of your

culture.

EVOS™M5000 Imaging System Quick Reference Guide

Auto Count Manual Count Confluence

Toggle pseudo color: Allows you to display images in pseudocolor or in grayscale in the

Viewing area. By default, color display is on.

Automate: Allows you to display the controls for the Time Lapse and Z-Stack

tools, and the EVOS™Onstage Incubator.

Time Lapse: Opens the Time Lapse tool, which allows you to

create and run time lapse routines to capture images at

given intervals over a time period based on your

specifications.

EVOS™M5000 Imaging System Quick Reference Guide

Z Stack: Opens the Z Stack tool, which allows you to capture

multiple images along the Z-axis based on your

specifications. You can use the images captured with the Z

Stack tool to generate a Z-Stack Projection.

Incubator: Opens the Incubator Controls, which allow you to

control the EVOS™Onstage Incubator and monitor its status

during your experiments.

Save: Opens the Save dialog, which allows you to select a

save location, set save options, and enable the Quick Save

option.

EVOS™M5000 Imaging System Quick Reference Guide

Review tab

Viewing area: Displays the captured image currently selected from the list of image files.

Folder: Displays the location of the

current folder or image.

Up: Goes up one folder from the current folder.

Refresh: Refreshes the list or grid of images in the current folder.

Grid view and List view buttons:

Display the folders or saved images in grid or list view.

Folder/Image display size:Increase and decrease the display size of the folders or

image files.

Browse:

Allows you to navigate to the folder

containing your saved images and to select the

image to display in the viewing area. Currently

selected folder or image is indicated with a box

around it.

Image properties: Displays the metadata for the

selected image file.

Display settings and analysis tools: Allow you to

change image display settings in the Viewing area,

and analyze and annotate captured images.

Export: Allows you to export the currently selected folder or image to a storage

device.

Save: Saves the currently opened image.

EVOS™M5000 Imaging System Quick Reference Guide

Settings

Settings tab

Objectives: Allows you to assign and unassign

objectives on the objective turret, and to

calibrate objective magnification.

Visuals:Allows you to calibrate color channel

lighting (White Balance Calibration) and to

assign saturated pixel colors in the

Fluorescence, Transmitted, and RGB

Transmitted channels.

General:Allows you to define saving options for

TIFF files and Saved Settings, and to reverse

the focus wheel action.

EVOS™M5000 Imaging System Quick Reference Guide

Filter Cubes:Allows you to add or remove

EVOS™LED light cubes from the instrument

and to assign pseudocolors to specific

channels.

Network: Allows you to connect to a Wi-Fi

network and to map network drives.

Service:Displays the EVOS

™

M5000 hardware

and software information, allows you to update

the EVOS™M5000 firmware and software from

the Thermo Fisher Cloud or using a USB flash

drive, and to copy the error logs.

Done: Closes the Settings tabs and returns to the previously opened tab.

EVOS™M5000 Imaging System Quick Reference Guide

Instrument exterior components and mechanical controls

Front view

LCD monitor

Condenser slider slot

Condenser head

Mechanical X-Y stage

Stage X-axis positioning knob

Stage Y-axis positioning knob

Objective selection wheel

Focusing knob

Vessel holder

Objective

Phase annuli selector

Rear view

LCD monitor

Mechanical X-Y stage

Focusing knobs

Power switch

4-pin power input port (12 VDC, 5 A)

Display port (video output)

Network port

USB-A 2.0 ports (4×)

USB 3.0 port (1×)

Stage X-axis positioning knob

Stage Y-axis positioning knob*

* Not visible in this perspective.

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