Thermo Scientific 3010 Instrucciones de funcionamiento

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www.thermoscientific.com/labwarranty

User Manual

Model 3010 and 3020

Water Jacketed CO2 Incubators

Operating and Maintenance Manual 7013010 Rev. 2

Thermo Scientific

MANUAL NUMBER 7013010

2 40087/IN-4743 7/27/16 Changed label on remote alarm artwork to note - pg 5-1 ccs

1 40639 5/3/16 Added risk assessment info ccs

0 27988/IN-4478 4/7/14 Release 2 - Original ccs

REV ECR/ECN DATE DESCRIPTION By

Preface

Water Jacketed CO2 Incubator i

Models covered:

Model Sensor* Voltage**

3010 T/C sensor 115V

3020 IR sensor 115V

*T/C is a thermal conductivity sensor, IR is an infra-red sensor.

**All units are 50/60Hz.

Thermo Scientificii Water Jacketed CO2 Incubator

Preface

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

CAUTION

Important Read this operating manual. Failure to read, understand and follow the instructions in this manual

may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Warning All internal adjustments and maintenance must be performed by qualified service personnel. s

Material in this manual is for information purposes only. The contents and the product it describes are subject

to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this

manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to

the use of this manual.

Intended Use

The Thermo Scientific Water Jacketed CO2incubators are intended to provide an environment with con-

trolled temperature and Carbon Dioxide (CO2) as well as elevated humidity (RH) for the growth of cultivat-

ing cell and tissue cultures.

Non Intended Use

The incubators are not intended for use by the general public. The units are not intended for use within the

patient environment or outdoors. These units are not designed to be used with liquid CO2 or liquid N2.

These units should not be used with any flammable or toxic materials.

Thermo Scientific Water Jacketed CO2 Incubator iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this

symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible

injury.

Asphyxiation Hazard Warning. High concentrations of CO2can displace oxygen and cause

asphyxiation!

Lifting Hazard Warning. The incubator weighs more than 200 lbs (91kgs). Take adequate safety

measures when moving this device.

WEEE Compliance: Thermo Fisher Scientific has contracted with companies for recycling/disposal

in each EU Member State. For further information, send an email to

[email protected].

4Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4Always dissipate extreme cold or heat and wear protective clothing.

4Always follow good hygiene practices.

4Each individual is responsible for his or her own safety.

Thermo Scientificiv Water Jacketed CO2 Incubator

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct

1-800-438-4851 Toll Free, U.S. and Canada

1-877-213-8051 FAX

http://www.thermofisher.com Internet Worldwide Web Home Page

service.led.marietta@thermofisher.com Tech Support Email Address

Certified Service Web Pagewww.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We canSales Support

take your order and provide delivery information on major equipment items or make

arrangements to have your local sales representative contact you. Our products are listed on the

Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation orService Support

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or

provide you with on-site service. We can also provide you with a quotation on our Extended

Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your

applications. If you are experiencing technical problems, working together, we will help you

locate the problem and, chances are, correct it yourself...over the telephone without a service

call.

When more extensive service is necessary, we will assist you with direct factory trained

technicians or a qualified service organization for on-the-spot repair. If your service need is

covered by the warranty, we will arrange for the unit to be repaired at our expense and to your

satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you

Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by

telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific (Asheville) LLC

401 Millcreek Road, Box 649

Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific Water Jacketed CO2 Incubator v

Preface

Warranty Notes

Information You Should Know Before Requesting Warranty Service

•Locate themodel and serial numbers.Aserial tag is located on the unit itself.

•For equipment service or maintenance, or with technical or special application inquiries, contact Technical

Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada).Outside the USA, contact your local

distributor.

Repairs NOT Covered Under Warranty

•Calibration of control parameters.Nominal calibrations are performed at the factory; typically ±1°Cfor

temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as

abillable service at your location. Calibration after awarranty repair is covered under the warranty.

•Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to

equipment materials.

•Service calls for improper installation or operating instructions. Corrections to any of the following

are billable services:

1)electrical service connection

2)tubing connections

3)gas regulators

4)gas tanks

5)unit leveling

6)room ventilation

7)adverse ambient temperature fluctuations

8)any repair external to the unit

•Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper

installation.

•Repairs to parts or systems resulting from unauthorized unit modifications.

•Any labor costs other than that specified during theparts and labor warranty period, which may

include additional warranty on CO2sensors, blower motors, water jackets, etc.

Water Jacketed CO2 Incubator viThermo Scientific

Table ofContents

Installation and Start-Up ......................................1-1

Control Panel Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Control Panel Keys, Displays & Indicators . . . . . . . . . . . . . . . . . . . .1-2

Unit Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

Stack the Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

Preliminary Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5

Shelf Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6

Air Sample Filter Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6

Access Port Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6

Connect Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7

Level the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7

Fill the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8

Fill the Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8

Connect the CO2Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9

Incubator Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10

Set the Operating Temperature Setpoint . . . . . . . . . . . . . . . . . . .1-10

Set the Overtemp Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-11

Set the CO2 Setpoint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-12

Calibration ..................................................2-1

Calibrate Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Calibrate Thermal Conductivity (T/C) CO2System . . . . . . . . . . . . .2-2

Calibrate Infra-Red (IR) CO2System . . . . . . . . . . . . . . . . . . . . . . . .2-3

Alarms ......................................................3-1

Section 1

Section 2

Section 3

vii Water Jacketed CO2 Incubator Thermo Scientific

Routine Maintenance .........................................4-1

Clean the Incubator Interior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Reverse the Door Swing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

Reverse the Hinges for Exterior Door . . . . . . . . . . . . . . . . . . . . . . .4-4

Clean the Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

Clean the Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

Replace Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-10

Replace the Access Port Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11

Replace the Air Sample Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-11

Add or Replenish Rust Inhibitor . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12

Drain Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-12

Remote Alarms ...............................................5-1

Specifications ...............................................6-1

Parts List ....................................................7-1

Electrical Schematics ........................................8-1

Warranty Information .........................................9-1

Table of Contents

Section 5

Section 6

Section 8

Section 7

Section 9

Section 4

Water Jacketed CO2 Incubator 1-1Thermo Scientific

Section 1 Installation and Start-Up

• Outer Door - Reversible to opposite swing, see Section 4.

• Heated Inner Door - Keeps chamber interior dry. Reversible to opposite

swing, see Section 4.

• Chamber Gas Sample Port - Used for sampling chamber CO2content,

for calibration (Section 2).

• Main Power Switch

• Control Panel - Keypad, Displays and Indicators. (See Figure 1-2).

• Fill Port - Used for filling the water jacket.

• Water Jacket Vent - Allows air to escape from the water jacket during

filling and normal expansion and contraction when the incubator heats

or cools.

• Leveling Legs - Used to level the unit.

• Water Jacket Drain – Remove plug and use hose barb insert included.

Plug when not in use.

Note The incubators are stackable. Information follows in this section. s

*When setting up the incubator, install the cover plate packed inside the

incubator shipping carton.

Heated Glass

Inner Door

Leveling

Legs (4)

Jacket

Vent

Water Jacket

Fill Port

Jacket

Drain

(removeplug before using)*

Control Panel

Chamber Gas

SamplePort

Power

Switch

Hose

Barb

Insert

OverTemp

LowTemp

HighCO2

LowCO2

AddWater

DoorOpen

MODE

SILENCE

Run

SetTemp

SetOtemp

SetCO2

CalTemp

CalCO2

ENTER

Inject

Heat Temp °C

%CO2

Figure 1-1.Water Jacket Incubator

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