Sutter Instrument Lambda 721 Manual de usuario

One Digital Drive

Novato, CA 94949

Voice: 415-883-0128 Web: www.sutter.com

Fax: 415-883-0572 Email: info@sutter.com

Lambda 721

Ultra-High-Speed Optical Beam Combined

7-Channel Wavelength Switching

LED Light Source System

With TTL & USB Interface for External Control

Operation Manual

Rev. 1.10c ((20210517)

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

LAMBDA-10®and SmartShutter®are registered trademarks of Sutter Instrument

Company.

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

iii

DISCLAIMER

The Lambda 721 is an ultra-high-speed wavelength switching multi-LED-based beam

combined illumination or light source device. The purpose of the system is to be an

illuminator for microscopes. No other use is recommended.

This instrument is designed for use in a laboratory environment. It is not intended, nor

should it be used in human experimentation or applied to humans in any way. This is not a

medical device.

Unless otherwise indicated in this manual or by Sutter Instrument Technical Support for

reconfiguration, do not open or attempt to repair the instrument.

Do not allow unauthorized and/or untrained operative to use this device.

Any misuse will be the sole responsibility of the user/owner and Sutter Instrument Company

assumes no implied or inferred liability for direct or consequential damages from this

instrument if it is operated or used in any way other than for which it is designed.

SAFETY WARNINGS AND PRECAUTIONS

Electrical

Operate the Lambda 721 using 110 – 240 VAC., 50-60 Hz line voltage. This instrument is

designed for use in a laboratory environment that has low electrical noise and mechanical

vibration. Surge suppression is recommended at all times.

Fuse Replacement: Replace only with the same type and rating:

5 x 20 mm glass tube, Time Lag (IEC 60127-2, Sheet 6)

T3.15A 250V (Time Lag, 3.15 Amps, 250 Volts)

(Littelfuse 02193.15MXAP (219XA Series, RoHS compliant))

A spare fuse is located in the power input module.

Avoiding Electrical Shock and Fire-related Injury

Always use the grounded power supply cord set provided to connect the system’s

power adapter to a grounded/earthed outlet (3-prong). This is required to protect you

from injury in the event that an electrical hazard occurs.

Do not disassemble the system. Refer servicing to qualified personnel.

To prevent fire or shock hazard do not expose the unit to rain or moisture.

Electromagnetic Interference

To comply with FDA and CE/EU electromagnetic immunity and interference standards; and

to reduce the electromagnetic coupling between this and other equipment in your lab always

use the type and length of interconnect cables provided with the unit for the interconnection

of its components (see the Technical Specifications appendix for more details).

Operational

Failure to comply with any of the following precautions may damage this device.

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

Operate only in a location where there is a free flow of fresh air on all sides.

NEVER ALLOW THE FREE FLOW OF AIR TO BE RESTRICTED.

This instrument is designed for operation in a laboratory environment (Pollution Degree

I) that is free from mechanical vibrations, and electrical noise and transients.

DO NOT CONNECT OR DISCONNECT THE LQUID LIGHT GUIDE BETWEEN

THE LAMBDA 721 AND MICROSCOPE ADAPTER (OR THE ADAPTER FROM THE

MICROSCOPE) WHILE POWER IS ON.

Operate this instrument only according to the instructions included in this manual.

Do not operate if there is any obvious damage to any part of the instrument.

Do not operate this instrument near flammable materials. The use of any hazardous

materials with this instrument is not recommended and, if undertaken, is done so at the

users’ own risk.

Do not operate if there is any obvious damage to any part of the instrument.

Avoiding Physical Injury while Powered up and Emitting Light

INFRARED AND ULTRAVIOLET RADIATION: Possible infrared and ultraviolet

radiation generated by this lamp can cause significant skin burns and eye damage.

Other

Retain the original packaging for future transport of the instrument.

Sutter Instrument reserves the right to change specifications without prior notice.

This device is intended only for research purposes.

WARNING

DO NOT LOOK DIRECTLY INTO THE OUTPUT OF THE LIGHT

APERTURE MOUNTED ON THE CONTROLLER, THE

CONNECTED LIQUID LIGHT GUIDE, OR THE ATTACHED

MICROSCOPE ADAPTER! Always direct the output of the light

aperture into the microscope using the appropriate adapters,

directed away from anyone’s eyes, and not directed toward any

reflective surface.

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

TABLE OF CONTENTS

DISCLAIMER ......................................................................................................................................iii

SAFETY WARNINGS AND PRECAUTIONS...................................................................................iii

Electrical .................................................................................................................................................iii

Avoiding Electrical Shock and Fire-related Injury.............................................................................iii

Electromagnetic Interference ...............................................................................................................iii

Operational .............................................................................................................................................iii

Avoiding Physical Injury while Powered up and Emitting Light .....................................................iv

Other........................................................................................................................................................iv

1. INTRODUCTION.............................................................................................................................1

1.1 Introduction.......................................................................................................................................1

1.1.1 How to Use this Manual............................................................................................................1

1.1.2 Technical Support ......................................................................................................................1

1.2 General Description..........................................................................................................................2

1.2.1 Features.......................................................................................................................................3

1.2.2 Common Applications................................................................................................................3

2. INSTALLATION ..............................................................................................................................5

2.1 Unpacking..........................................................................................................................................5

2.2 Getting Started..................................................................................................................................5

2.3 Configuration ....................................................................................................................................7

DIP Switches ........................................................................................................................................7

3. OPERATIONS ..................................................................................................................................9

3.1 Controls..............................................................................................................................................9

3.1.1 Power Switch..............................................................................................................................9

3.2 Indicators ...........................................................................................................................................9

3.2.1 LED On/Off State Indicators ....................................................................................................9

3.3 Connectors .........................................................................................................................................9

3.3.1 Light Beam Combiner Output..................................................................................................9

3.3.1 TTL Inputs (LED # 1 – 7) BNC Connectors ........................................................................10

3.3.1 Ring Buffer BNC Connector ...................................................................................................10

3.3.2 USB Device Interface Connector............................................................................................10

3.4 Operation .........................................................................................................................................10

3.1 Lambda 10 Mode.............................................................................................................................10

3.1.1 Control Operations...................................................................................................................11

3.1.2 Mode Exit ..................................................................................................................................11

3.1 TTL Mode ........................................................................................................................................11

3.1.1 Control Operations...................................................................................................................12

3.1.1 Mode Exit ..................................................................................................................................12

3.2 Ring Buffer Mode............................................................................................................................12

3.2.1 Running the Ring Buffer Control Operations ......................................................................13

3.2.2 Stopping the Ring Buffer Run ................................................................................................13

3.2.1 Editing the Ring Buffer ...........................................................................................................13

3.1 Setup Mode ......................................................................................................................................13

3.1 Demo Mode......................................................................................................................................14

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

4. EXTERNAL CONTROL OPERATIONS......................................................................................15

4.1 USB Interface..................................................................................................................................15

4.1.1 Virtual COM Port (VCP) Serial Port Settings......................................................................15

4.2 Handshaking ...................................................................................................................................15

4.3 Commands .......................................................................................................................................16

4.3.1 Lambda 10-x Mode (‘L’ or ‘l’) Command ..............................................................................16

4.3.1.1 Lambda 10 Series Compatible LED Selection Commands...........................................16

4.3.1 TTL Mode (‘T’ or ‘t’) Command.............................................................................................18

4.3.1 Start Ring Buffer Run (‘R’ or ‘r’) Command ........................................................................19

4.3.1.1 Return Values while Running the Ring Buffer..............................................................19

4.3.1 Stop TTL Mode or Ring Buffer Run (‘O’ or ‘o’) Command.................................................19

4.3.1 Load Ring Buffer (‘B’ or ‘b’) Command.................................................................................20

4.3.1.1 ‘B’ or ‘b’ Command Stop Loading Ring Buffer Entry Value........................................20

4.3.1.1 ‘B’ or ‘b’ Command Ring Buffer Entry Values ..............................................................20

4.3.1 Turn on a discrete LED or a group of LEDs (‘M’ or ‘m’) Command .................................21

4.3.1 Set LED Power Level (‘P’ or ‘p’) Command .........................................................................22

4.3.1 Get LED ON/OFF Status (‘S’ or ‘s’) Command ...................................................................22

4.3.1 Get Lambda 10-3 Compatible Controller Type and Configuration Command.................22

4.3.1 Get Lambda 10-3 Compatible Status Command..................................................................23

5. MAINTENANCE AND RECONFIGURATION...........................................................................25

5.1 Routine Maintenance .....................................................................................................................25

5.2 Reconfiguration...............................................................................................................................25

5.2.1 Replacing and Installing New LED Cubes ............................................................................25

5.2.2 Setting up the Maximum Current Level for each LED .......................................................25

APPENDIX A. LIMITED WARRANTY............................................................................................27

APPENDIX B. ACCESSORIES .........................................................................................................29

B.1. Liquid Light Guides, SMA Fiber, and Other Accessories.........................................................29

B.2. Microscope Mounting Adapters...................................................................................................29

B.3. LED Cubes .....................................................................................................................................29

APPENDIX C. FUSE REPLACEMENT...........................................................................................31

APPENDIX D. TECHNICAL SPECIFICATIONS ...........................................................................33

APPENDIX E. QUICK REFERENCE ..............................................................................................35

E.1. Manual Operation.........................................................................................................................35

E.2. Configuration.................................................................................................................................36

E.3. External Control............................................................................................................................36

INDEX .................................................................................................................................................49

TABLE OF FIGURES

Figure 1-1. Lambda 721 indicators, controls, and connectors............................................................... 2

Figure 1-2. Stages of pass-through and reflection from each LED cube light source......................... 3

Figure 1-3. Lambda 721 LED Spectra Options. ...................................................................................... 4

Figure 2-1. Light guide installation. ......................................................................................................... 5

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

vii

Figure 2-2. Rear panel showing power entry module receptacle and switch....................................... 6

Figure 2-1. Rear panel showing connectors for TTL input for all 7 LED channels, Ring Buffer

strobe input, and USB for external control. .......................................................................... 7

Figure 2-2. Left-side panel and DIP switches.......................................................................................... 7

Figure 3-1. Locations of individual indicators, controls, and connectors on the Lambda 721

controller front panel. .............................................................................................................. 9

Figure 3-2. Locations of power switch and individual connectors on the Lambda 721 controller

rear panel................................................................................................................................... 9

Figure 3-3. Startup screen. ...................................................................................................................... 10

Figure 3-4. Product and firmware revision screen. .............................................................................. 10

Figure 3-5. Lambda 10 Mode prompt..................................................................................................... 10

Figure 3-6. Lambda 10 Mode prompt..................................................................................................... 11

Figure 3-7. Lambda 10 Mode screen....................................................................................................... 11

Figure 3-8. Use TTL Mode prompt......................................................................................................... 11

Figure 3-9. TTL Mode start screen......................................................................................................... 11

Figure 3-10. TTL Mode running screen................................................................................................. 12

Figure 3-11. Use Ring Buffer prompt..................................................................................................... 12

Figure 3-12. Run existing Ring Buffer prompt. .................................................................................... 12

Figure 3-13. Ring buffer running screen................................................................................................ 12

Figure 3-14. Edit ring buffer query screen. ........................................................................................... 13

Figure 3-15. Ring buffer entry #1 screen. ............................................................................................. 13

Figure 3-16. Setup Mode prompt screen................................................................................................ 13

Figure 3-17. Edit ring buffer query screen. ........................................................................................... 14

Figure 3-18. Demo Mode prompt screen................................................................................................ 14

Figure 3-19. Enter Demo Mode prompt screen..................................................................................... 14

Figure 3-20. Demo Mode running screen............................................................................................... 14

Figure 5-1. LED cube placement. ........................................................................................................... 25

Figure 5-2. Setting the maximum current for an LED. ....................................................................... 26

Figure 5-3. Prompt for finishing setting LED current levels. ............................................................. 26

Figure 5-4. Message to turn off unit and reset DIP Switch 8 position before turning unit back on.26

Figure 5-5. Lambda 10 Mode prompt..................................................................................................... 26

Figure C-1. Power entry module and fuse detail................................................................................... 31

Figure E-1. Lambda 721 front panel and controls................................................................................ 35

Figure E-2. Lambda 721 menu navigation diagram............................................................................. 35

LAMBDA 721 OPERATION MANUAL – REV. 1.10C (20210517)

viii

Figure E-3. Lambda 721 rear panel, connectors, and controls............................................................ 36

Figure E-4. Lambda 721 left-side panel and DIP switches. .................................................................36

Figure E-5. LED cube placement............................................................................................................ 36

TABLE OF TABLES

Table 2-1. Configuration Switches 1 – 8 definitions. .............................................................................. 8

Table 4-1. USB-VCP interface serial port settings. .............................................................................. 15

Table 4-2. Lambda 10-x Mode (‘L’ or ‘l’) Command............................................................................. 16

Table 4-3. Lambda 10 Series compatible LED selection commands................................................... 17

Table 4-4. LED selection commands using ASCII digits...................................................................... 18

Table 4-5. TTL Mode (‘T’ or ‘t’) Command........................................................................................... 18

Table 4-6. Start Ring Buffer Run (‘R’ or ‘r’) Command....................................................................... 19

Table 4-7. Return values while running the Ring Buffer..................................................................... 19

Table 4-8. Stop TTL Mode or Ring Buffer Run (‘O’ or ‘o’) Command............................................... 19

Table 4-9. Load Ring Buffer (‘B’ or ‘b’) Command............................................................................... 20

Table 4-10. ‘B’ or ‘b’ Command Stop Loading the Ring Buffer entry value..................................... 20

Table 4-11. ‘B’ or ‘b’ Command Ring Buffer entry values................................................................... 20

Table 4-12. Change the on/off state of one or more LEDs (‘M’ or ‘m’) Command............................ 21

Table 4-13. Set LED Power Level (‘P’ or ‘p’) Command. .................................................................... 22

Table 4-14. Get LED ON/OFF Status (‘S’ or ‘s’) Command................................................................ 22

Table 4-15. Get Lambda 10-3 Compatible Controller Type and Configuration Command. ............ 23

Table 4-16. Get Lambda 10-3 Compatible Controller Type and Configuration Command. ............ 24

Table 5-1. Cable specifications. ............................................................................................................... 34

Table E-1. Configuration Switches 1 – 8................................................................................................ 36

Table E-2. Serial port settings................................................................................................................. 37

Table E-3. Lambda 721 external control commands............................................................................ 38

Table E-4. Lambda 10 Series compatible LED selection commands.................................................. 41

Table E-5. LED selection commands using ASCII digits. .................................................................... 42

Table E-6. Ring Buffer entry values....................................................................................................... 43

Table E-7. Return values while running the Ring Buffer....................................................................

Table E-8. ‘M’ Command 1st argument (2nd byte) values for individual LED ON/OFF state bit

encoding................................................................................................................................... 44

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