StatSpin ThermoBrite S500-12 Manual de usuario

Service Manual

ThermoBrite

Model Number S500-12/S500-24

FOR IN VITRO DIAGNOSTIC USE

StatSpin is a registered trademark of StatSpin Inc., a Subsidiary of IRIS International, Inc.

Printed in U.S.A.

Table of Contents

Table of Contents............................................................................................................ i

How to use this manual.....................................................................................iii

Section 1.............................................................................................................1

Safety................................................................................................................................. 1

Before you Begin...................................................................................................... 1

Install System............................................................................................................ 1

Connect Power ......................................................................................................... 1

Section 2.............................................................................................................2

System Overview ........................................................................................................... 2

Principle and Intended Use .................................................................................... 2

Symbols and Definitions.......................................................................................... 2

Symbols and Definitions (cont.) ............................................................................. 3

Display Abbreviations .............................................................................................. 3

Audible Indicators - NORMAL ................................................................................ 3

Audible Indicators - ERROR................................................................................... 3

Error Messages ........................................................................................................ 4

Section 3.............................................................................................................5

Operating Instructions.................................................................................................. 5

Opening and Closing the Lid .................................................................................. 5

Turning Unit On ........................................................................................................ 5

Run a Denaturation and Hybridization Program ................................................. 5

Abort Program in Process....................................................................................... 7

Slide Installation ....................................................................................................... 7

Humidity Control Cards ........................................................................................... 7

Predefined Limits...................................................................................................... 7

Section 4.............................................................................................................8

Specifications ................................................................................................................. 8

Table........................................................................................................................... 8

Section 5.............................................................................................................9

Theory of Operation ...................................................................................................... 9

Overview.................................................................................................................... 9

Data Acquisition........................................................................................................ 9

Calibration ................................................................................................................. 9

PID............................................................................................................................ 10

Section 6...........................................................................................................11

Alignment....................................................................................................................... 11

Materials .................................................................................................................. 11

Workstation Setup.................................................................................................. 11

Procedure ................................................................................................................ 11

Calibration ............................................................................................................... 12

Testing ..................................................................................................................... 13

Section 7...........................................................................................................15

Programming................................................................................................................. 15

Overview.................................................................................................................. 15

Character Selection ............................................................................................... 15

Creating a Denaturation and Hybridization Program (Denat & Hyb) ............. 15

Section 8...........................................................................................................16

Schematic....................................................................................................................... 16

Block Diagram ........................................................................................................ 16

Main PCB................................................................................................................. 17

Main PCB................................................................................................................. 18

Section 9...........................................................................................................19

Disassembly .................................................................................................................. 19

Heater Assembly .................................................................................................... 19

Housing Assembly ................................................................................................. 20

Section 10 .........................................................................................................21

Troubleshooting........................................................................................................... 21

Section 11 .........................................................................................................22

Exploded View .............................................................................................................. 22

ThermoBrite............................................................................................................. 22

Part Numbers.......................................................................................................... 23

iii

How to use this manual

This manual along with information contained on product labels should provide you with all the

information you need to operate and maintain the ThermoBrite.

Notes appear in italics to highlight information. When the information requires special attention, a

caution symbol appears next to the italicized text.

Please pay close attention to the instructions that accompany the notes and symbols as well as the

standard laboratory practices outlined by your facility and local regulatory agencies. The table below

lists all the CAUTIONS / WARNINGS for the ThermoBrite.

WARNING – Plug the instrument into a properly grounded outlet supplying the

voltage and frequency indicated on the serial number label.

CAUTION – Unplug the ThermoBrite from the wall outlet before performing

maintenance.

WARNING – Do not expose ThermoBrite to strong or concentrated acids, bases,

esters, aromatic or halogenated hydrocarbons, ketones or strong oxidizing agents.

CAUTION - Universal Precautions should be followed on all specimens, regardless

of whether a specimen is known to contain an infectious agent. (See references)

CAUTION –Risk of electric shock: The instrument contains no user serviceable

parts. Removal of housing will expose potentially lethal voltage. Refer service to

qualified service personnel.

CAUTION – Hot Surface: The interior surface of the instrument may be HOT, use

caution to avoid potential burn.

Please use the system as intended. Improper use of the ThermoBrite may cause damage to the

system, inaccurate results, or potentially void warranties.

Section 1

Safety

Before you Begin

READ ALL PRODUCT MANUALS AND CONSULT WITH TRAINED PERSONNEL BEFORE

ATTEMPTING TO OPERATE OR SERVICE THIS INSTRUMENT.

HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

WARNINGS, CAUTIONS and IMPORTATANT alert you as follows:

WARNING - Might cause injury.

CAUTION - Might cause damage to the instrument.

IMPORTANT - Might cause misleading results

CAUTION: System integrity might be compromised and operational failures might occur if:

This equipment is used in a manner other than specified. Operate the instrument as

instructed in the Product Manuals.

You introduce software that is not authorized by StatSpin into the instrument. Only operate

your system with software authorized by StatSpin.

You install software that is not an original copyrighted version. Only use software that is an

original copyrighted version to prevent virus contamination.

IMPORTANT: StatSpin urges its customers to comply with all national health and safety standards

such as the use of barrier protection. This may include, but it is not limited to protective eyewear,

gloves and suitable laboratory attire when operating or maintaining this or any other automated

laboratory analyzer.

Install System

1. Place the ThermoBrite on a level surface suitable for laboratory instrumentation.

2. ThermoBrite has an intake fan located on bottom, assure no obstruction exist on intake.

3. Ensure the ThermoBrite is placed at least 12” (0.3048m) from the wall to allow for proper

cooling.

4. Position the ThermoBrite away from direct sunlight and sources of heat or cold.

5. Verify voltage requirements located on serial number label on the rear of instrument.

Connect Power

Plug the instrument into a grounded outlet supplying the voltage and frequency indicated on the

serial number label.

Main power switch is located on the rear of the instrument, next to the line cord power entry

module.

Section 2

System Overview

Principle and Intended Use

For in vitro diagnostic use for denaturation/hybridization for slide-based FISH procedures.

The ThermoBrite is a microprocessor controlled small bench top hot plate with lid. The ThermoBrite

allows storage of 40 programs, three operating modes, fixed temperature, Hybridization only or

Denaturing and Hybridization. Capacity for twelve slides and a maximum temperature of 99°C. The

instrument is UL / CUL listed and CE marked.

Symbols and Definitions

Up Move cursor up; Enter character A-Z for program

name

Down Move cursor down; Enter character A-Z for

program name

Enter Accept or Enter

Backspace Move cursor back or previous screen

Stop End a program in process

0-9 Enter numeric values for time and temperature or

for program name

REF Product/Reference

Number

Indicates the StatSpin product/catalog number

Caution Statement of caution/warning, read instruction

carefully

Temperature

limitation

Indicates storage requirements range

EC Representative European Community Authorized Representative

IVD

For in vitro

diagnostic use

Clarifies for use as in vitro diagnostic use only

Non-sterile Indicates non-sterile product

S N Serial Number Indicates instrument serial number code

Consult

Instructions

Consult instruction manual for further explanation

IVD

Symbols and Definitions (cont.)

Manufactured By Indicates Manufacturer of the Device

Electric Shock Indicates a potential risk of electrical shock

Biohazard Universal Precautions should be followed at all times

Hot Surface Use caution to avoid burn

Display Abbreviations

PGM Program

Denat & Hyb Denaturation & Hybridization

Denat Temp Denaturation Temperature

Denat Time Denaturation Time

Hyb Temp Hybridization Temperature

Hyb Time Hybridization Time

Hyb Only Hybridization Only

Fixed Temp/Fxd Fixed Temperature

Audible Indicators - NORMAL

Single beep: All legal keystrokes.

Two quick beeps: Upon accepting a field and cursor has moved to next field.

Five beeps: Completion of process.

Audible Indicators – ERROR

Three short beeps: Entering of illegal or non-functioning keystrokes.

Low tone beep: Attempt to enter a value out of acceptable range.

Continuous beep: Instrument is not performing within acceptable range or program

condition. Turn off main power and restart. If beep continues,

discontinue use and contact service.

Error Messages

Instrument cannot achieve a set temperature by heating within 10 minutes an error message appears to inform user to

turn unit off and call service. A constant beep will sound.

If the instrument cannot measure the temperature, the software will automatically turn off the heating. An error message

will appear to inform the user to turn off the unit and call service. A constant beep will sound.

Instrument cannot achieve a set temperature by heating within 10 minutes an error message appears to inform user to

turn unit off and call service. A constant beep will sound.

High ambient temperature condition:

The instrument will attempt to achieve process set temperatures. However, if the cooling fan cannot achieve the

set temperature within 10 minutes, an error message will appear to inform the user that the ambient temperature is

high. A constant beep will sound. The counter will continue to count. The present temperature will be displayed.

Hitting the “Stop” button will allow the user to abort the process. A new screen will be displayed asking user if they

are sure they want to abort.

Please Wait

Cooling to XXX --°C

Present Temp: --°C

If the ambient temperature changes during a process and causes the instrument process set temperature to change

beyond the +/- 1°Cspecification for more than 2 minutes, a message will appear to inform the user that the

ambient temperature is high. A constant beep will sound. The counter will continue to count. The present

temperature will be displayed. Hitting the “Stop” button will allow user to abort the process. A new screen will be

displayed asking user if they are sure they want to abort.

If the ambient temperature changes after a process is completed, but before the user removes the slides and

causes the instrument process set temperature to change beyond the +/- 1°Cspecification for more than 2

minutes a message will appear to inform the user that the ambient temperature is high. A constant beep will sound.

The counter will continue to count. The present temperature will be displayed. Hitting the “Stop” button will allow

user to abort the process. A new screen will be displayed asking user if they are sure they want to abort.

Note: If 40 programs have been created or edited the software will blank out the “Create” mode on the main menu

screen. This will only allow users to edit existing programs.

SYSTEM ERROR!

TURN UNIT OFF!

CALL SERVICE

Please Wait

Cooling to XXX --°C

Ambient Temp High!

PGM -- namexxxxxx

“Ambient Temp High!”

Hyb --°C --:--

Present Temp: --°C

PGM -- namexxxxxx

“Ambient Temp High!”

Reset Timer 00:00:00

End PGM/Main Menu

PGM -- namexxxxxx

“Ambient Temp High!”

Total Hyb Time --:--

End PGM/Main Menu

Run a PGM

Edit a PGM

Present Temp: --°C

Section 3

Operating Instructions

Opening and Closing the Lid

The platen may be hot. Use caution and check temperature on display before handling slides. Improper

precaution can cause a burn.

Depressions located on either side of the lid allow user to simply lift lid into position. The lid should offer

some resistance when opening. To close, reverse process. Assure front is completely down and no

obstructions prevent gasket seal from sealing on housing base.

Turning Unit On

The ThermoBrite main power switch is located on the rear panel. Assure unit is plugged into a grounded

outlet. Move switch to on position. Instrument will beep to announce power has been turned on, fan will

start and the Main Menu will come up onto the display.

I = ON O=OFF

Run a Denaturation and Hybridization Program

Turn unit on and wait for the Main Menu screen. Cursor highlights “Run a PGM” line.

Press “Enter” button to accept.

With the arrow keys scroll through program numbers 1 to 40 / program names. If no programs have been

saved advance to programming section of this handbook. To accept, press “Enter” button.

Display will confirm PGM number/name and Denat & Hyb times and temperatures. Cursor highlights “Run

PGM” line. Press “Enter” button to accept.

Hyb Only Denat & Hyb Fxd Temp

PGM 01 Her2

82°C :05; 45°C 16:00

Run PGM

Main Menu

The display prompts to “Add Slides and Close Lid”. Before adding slides, insert two Humidity Cards into the

inside lid. After card insertion saturate with distilled water or equivalent (see Humidity Control Cards).

Cursor highlights “Start” line. Press “Enter” button to run the program.

(To return to the Main Menu, move the cursor to highlight “Main Menu” line and press “Enter” button).

Run a PGM

Edit a PGM

Create a PGM

Present Temp: 37°C

PGM 02 EBV

Hyb: 55°C 01:30

Run PGM

Main Menu

PGM 03 Appl

Fixed: 65°C

Run PGM

Main Menu

PGM 03 Appl

Add Slides - Close Lid

Start

Main Menu

PGM 02 EBV

Add Slides - Close Lid

Start

Main Menu

PGM 01 Her2

Add Slides - Close Lid

Start

Main Menu

Este manual sirve para los siguientes modelos

Tabla de contenidos

Manuales populares de Equipo de laboratorio de otras marcas

Qiagen

Qiagen DML 3000 Manual de usuario

Queensgate

Queensgate NANOSCAN OP400 Manual de programación

Conviron

Conviron GEN1000-GE Manual de usuario

Parr Instrument

Parr Instrument 4560 Manual de instrucciones

IKA

IKA IC control Manual de usuario

Integra

Integra DOSE IT Manual de usuario

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manual de usuario

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manual de usuario

NI PXI-5422 Manual

Collomix

Collomix Aqix Manual de usuario

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manual de usuario

Ocean Insight

Ocean Insight FLAME-NIR+ Manual de usuario

Parker

Parker ALIGN-MG-NA Manual de usuario

BD 644787 Manual de usuario

DENTAURUM

DENTAURUM Compact Megaplus Manual de usuario

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manual de usuario

VWR

VWR SAS Super IAQ Manual de usuario

illumina

illumina MiSeqDx Manual de usuario