Star Lab RepeatOne Manual de usuario

Liquid HandLing TecHnoLogy

User Manual · Bedienungsanleitung · Mode d’emploi · Instruzioni d’impiego

www.starlabgroup.com

User Manual _______________________________ 3

Bedienungsanleitung _______________________ 15

Mode d’emploi ____________________________ 27

Instruzioni d’impiego_______________________ 39

English

RepeatOne · User Manual · 3

1. General ____________________________________ 4

1.1. Design Principle of RepeatOne® ____________________ 4

2. Volume Selection and Choice of TipOne® Repeat ___ 5

2.1. TipOne® Repeat Dispenser Tips _____________________ 6

2.2. 25 ml and 50 ml TipOne® Repeat Dispenser Tips ________ 6

3. Operation ___________________________________ 7

3.1. Inserting TipOne® Repeat Dispenser Tips ______________ 7

3.2. Filling TipOne® Repeat Dispenser Tips ________________ 7

3.3. Dispensing ___________________________________ 8

3.4. Removing TipOne® Repeat Dispenser Tips _____________ 9

4. Safety Precautions and Operational Limitations ___ 9

5. Troubleshooting ____________________________ 10

6. Maintenance, Service and Warranty ____________ 11

7. Ordering Information ________________________ 12

8. Technical Data ______________________________ 13

9. Quick Reference Guide _______________________ 14

User Manual

4· RepeatOne · User Manual

1. General

RepeatOne® is a hand-held device for repetitive pipettings.

Up to 48 pipettings of the same sample can be made from

a single filling, resulting in a considerable reduction in time

and expense.

RepeatOne® allows the selection of five separate volumes

from each TipOne® Repeat Dispenser Tip. Eight different

TipOne® Repeat Dispenser Tip sizes provide the most

important volumes from 2 to 5000 µl.

Use only original STARLAB TipOne® Repeat Dispenser Tips

to guarantee the pipette’s performance.

1.1. Design Principle of RepeatOne® ( Fig.1)

Volume selection dial

To determine the pipetting volume, the dial setting

(1–5) is multiplied by the minimum pipetting volume

of the TipOne® Repeat Dispenser Tip.

Pipetting lever

The volume is dispensed by pressing the pipetting

lever down until it stops.

Filling lever

The TipOne® Repeat Dispenser Tip is filled by sliding

this lever upward.

Locking clamp

The locking clamp secures the TipOne® Repeat

Dispenser Tip firmly into RepeatOne®.

TipOne® Repeat Dispenser TipFig.1

English

RepeatOne · User Manual · 5

2. Volume Selection and Choice of TipOne® Repeat

For repetitive pipetting of a particular volume:

•Find the desired volume in the columns on the right.

•The required dial setting and maximum number of

pipetting steps for the volume are shown at the top

respectively buttom of the columns.

•Select the corresponding TipOne® Repeat Dispenser Tip

from the column on the left

*Adapter

necessary for

TipOne® Repeat

Dispenser Tip

25 ml and 50 ml.

Application Guide for TipOne® Repeat Dispenser Tips

TipOne® Repeat

Volume

Volume selection dial

12345

0.1 ml 2 μl 4 μl 6 μl 8 μl 10 μl

0.5 ml 10 μl 20 μl 30 μl 40 μl 50 μl

1.0 ml 20 μl 40 μl 60 μl 80 μl 100 μl

2.5 ml 50 μl 100 μl 150 μl 200 μl 250 μl

5.0 ml 100 μl 200 μl 300 μl 400 μl 500 μl

10 ml 200 μl 400 μl 600 μl 800 μl 1000 μl

25 ml* 500 μl 1.0 ml 1.5 ml 2.0 ml 2.5 ml

50 ml* 1.0 ml 2.0 ml 3.0 ml 4.0 ml 5.0 ml

48 23 15 11 8

Pipetting Steps

6· RepeatOne · User Manual

2.1. TipOne® Repeat Dispenser Tips

The minimum pipetting volume and the maximum

filling capacity is stated on each TipOne® Repeat

Dispenser Tips (Fig. 2). The scale facilitates drawing off

partial quantities and helps you to estimate the amount

of liquid still available for operation.

The volume to be pipetted is obtained by multiplying

the set number located on the volume selection dial by

the minimum pipetting volume of the TipOne® Repeat

Dispenser Tips.

Eg. 1.0 ml TipOne® Repeat Dispenser Tips, Minimum volume:

1=20 µl at setting 4: 4 x 20 µl = pipetted volume: 80 µl

Better precision is achieved by using a TipOne® Repeat

Dispenser Tips of small capacity with a high dial setting.

2.2. 25 ml and 50 ml

TipOne® Repeat

Dispenser Tips (Fig.3)

Attach the adapter to the

25 ml or 50 ml TipOne®

Repeat Dispenser Tips by

turning it clockwise; re-

move by turning it counter

clockwise.

Number

of Pipetting

Steps

Position

of Volume

Selection Dial

48 1

23 2

15 3

11 4

8 5

Fig. 3

The correlation between

the position of the volume

selection dial and the

number of pipetting steps

is as shown in the table.

Fig. 2

English

RepeatOne · User Manual · 7

Fig. 4

3. Operation

3.1. Inserting TipOne®

Repeat Dispenser Tips

•Slide the filling lever

down until it stops.

•Raise the locking clamp

upward.

•Insert the TipOne®

Repeat tip until it clicks

into position. Ensure

the TipOne® Repeat’s

plunger is fully inserted

into the barrel before

attaching it to the

RepeatOne® (Fig. 4). Fig. 5

•Ensure the filling lever is

completely down, and

then lower the locking

clamp to secure the

TipOne® Repeat tip in

place (Fig. 5).

3.2. Filling TipOne®

Repeat Dispenser Tips

Use the following steps

forall sizes of tips. 0.1 ml,

0.5 ml, 1.0 ml, and 2.5 ml

tips may also be used

with a standard 200 µl

pipette tip. Attaching a

tip facilitates filling from Fig. 6

narrow-necked vessels

or piercing of sealing foil

and also makes dispensing

of small volumes more

precise.

•Immerse the tip into the

liquid.

•Fill by slowly sliding the

filling lever upward

(Fig. 6).

•Discard the first

pipetting step.

RepeatOne® is now ready

for operation.

8· RepeatOne · User Manual

Additional Information

Sliding the filling lever too quickly can cause excessive

vacuum. This can cause tiny air bubbles to accumulate in

the liquid which may lead to dispensing inaccuracies.

Important: The first dispensing step must be discarded in

order to release any residual pressure from the dispensing

system after filling and to prepare the system for precise

dispensing.

Small bubbles in the TipOne® Repeat Dispenser Tip be-

neath the piston will not affect dispensing accuracy since

the residual stroke lock prevents the dispensing of any

remaining sample after the last dispense.

It is not necessary to completely fill the TipOne® Repeat

Dispenser Tip. Partially filling will not cause pipetting

inaccuracies.

3.3. Dispensing (Fig. 7)

•Check the volume

selection dial.

•Wipe the tip of the

TipOne® Repeat

Dispenser Tip with

lint-free tissue.

•Hold the dispensing tip

so it touches the inner

wall of the vessel.

•Dispense the liquid by

completely depressing

the dispensing lever.

Additional Information

Dispensing with a 5.0 ml or 10 ml TipOne® Repeat

Dispenser Tip is normally carried out without attaching

a pipette tip. Never attach a standard tip when working

with the 25 ml and 50 ml TipOne® Repeat tips.

A new TipOne® Repeat Dispenser Tip must be used each

time to avoid carry-over and contamination.

Fig. 7

English

RepeatOne · User Manual · 9

3.4. Removing TipOne® Repeat Dispenser Tips (Fig. 8)

•Hold RepeatOne® over a beaker.

•Slowly depress the filling lever down to the stop.

This will completely empty the TipOne® Repeat

Dispenser Tip.

•Raise the locking clamp upward.

•Remove the TipOne® Repeat Dispenser Tip.

Note: When using the 25 ml or 50 ml TipOne® Repeat

Dispenser Tips, the reusable adapter is removed by turning

it counter clockwise.

Fig. 8

4. Safety Precautions and Operational Limitations

When removing TipOne® Repeat Dispenser Tips, please

note the following:

•Do not touch the tip end, especially when using

hazardous reagents!

•Empty the dispenser tips into a proper discard receptacle

to avoid splashing and aerosols.

•Treat any retained fluid as a bio-hazard. Do not allow the

fluid to come into contact with laboratory personnel.

•If dispensing a liquid with a surface tension significantly

different from water, inaccuracies or leaking can occur.

•The RepeatOne® with an attached, partially filled

TipOne® Repeat Dispenser Tip should be stored in a

stand to prevent air entering the dispenser tip.

10 · RepeatOne · User Manual

5. Troubleshooting

Error Cause Solution

TipOne® Repeat Dispenser Tip cannot be inserted. Filling lever not fully in the down position;

therefore, locking clamp cannot be completely raised. Depress the filling lever downward and raise the locking clamp as far as it

will go.

The dispenser tip piston is not completely inserted in the barrel. Slide the piston into the proper position.

Unable to fully lift the filling lever. Locking clamp incompletely depressed. Completely depress the locking clamp.

Excessive air bubbles when filling the TipOne® Repeat Dispenser Tip. Filling lever lifted slightly before immersing into the liquid. Draw up some solution and then discard it (using filling lever).

Then refill the TipOne® Repeat Dispenser Tip.

Leaking of TipOne® Repeat Dispenser Tip. Replace the dispenser tip with a new one.

Inconsistent pipetting. Volume selection dial has not clicked into position. Click volume selection dial into position.

Dripping TipOne® Repeat Dispenser Tip. Leaking TipOne® Repeat Dispenser Tip. Replace with a new dispenser tip.

Attached TipOne® Repeat Dispenser Tip is loose. Use more force to attach the TipOne® Repeat Dispenser Tip.

RepeatOne® jams or does not function. Return the RepeatOne® for repair or replacement.

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