Sartorius Proline Manual de usuario

User Manual

Bedienungsanleitung

Mode d'emploi

Manual Usuario

Instruzioni d'impiego

Инструкция пользователя

Proline®pipette

User Manual ............................................................1

Bedienungsanleitung .......................................... 15

Mode d’ emploi ..................................................... 29

Manual Usario ....................................................... 43

Instruzioni d’impiego .......................................... 57

Инструкция пользователя

................................. 73

Specifications ........................................................ 88

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Proline®pipette

Contents

1. Intended use .....................................................................................................2

2. Product description........................................................................................2

2.1. Sartorius Proline adjustable volume pipettes ....................... 2

2.2. Sartorius Proline fixed volume pipettes.................................. 3

2.3. Sartorius pipette tips......................................................................3

3. Unpacking.......................................................................................................... 4

4. Installing the pipette holder....................................................................... 4

5. Pipette Materials.............................................................................................5

6. Operating the pipette....................................................................................6

6.1. Volume setting..................................................................................6

6.2. Sealing and ejecting tips...............................................................6

6.3. Two-in-one pipettes .......................................................................6

6.4. Protective filters ..............................................................................7

7. Pipetting techniques...................................................................................... 7

7.1. Forward pipetting............................................................................7

7.2. Reverse pipetting.............................................................................8

8. Storage................................................................................................................8

9. Testing the performance and recalibration ..........................................8

9.1. Testing the performance............................................................... 9

9.2. Recalibration procedure..............................................................11

10. Maintenance...................................................................................................11

10.1. Daily cleaning of the outer surface of the pipette ...........11

10.2. In-house maintenance.................................................................12

11. Troubleshooting.............................................................................................13

12. Warranty information.................................................................................14

1. Intended use

Proline pipette is intended to be used in liquid

handling applications for dispensing liquids

in total volume range from 0,1 µl to 5 ml. It is

recommended to use Optifit Tips or SafetySpace™

Filter tips to ensure the best compatibility and

performance with Sartorius pipettes.

This liquid handling device is designed and

manufactured to be used as general purpose

laboratory equipment. Before operating it is

recommended to read the user manual, which

contains useful information e.g. for good

pipetting practice.

2. Product description

Your new Proline hand-held pipette is a general

purpose pipette, for the accurate and precise

sampling and dispensing of liquid volumes. All

Proline pipettes operate on the basis of the air

displacement principle and use disposable tips.

The range of Sartorius Proline pipettes cover a

volume range from 0.1 µl to 5 ml.

2.1. Sartorius Proline adjustable

volume pipettes

Single channel pipettes:

Cat. No. Volume

Range/µl

Increment /

µl

Tip/

µl

Safe-Cone Filters

50 pcs/bag

Standard Plus

720010 0.1-2.5 0.05 10 - -

720015 0.5-10 0.1 10, 200 - -

720080 2-20 0.5 200 721008 721018

720025 5-50 0.5 200, 350 721008 721018

720050 10-100 1200, 350 721007 721017

720070 20-200 1200, 350 721007 721017

720060 100-1000 51000 721006 721016

720110 1000-5000 50 5000 721005 721015

Multichannel pipettes:

2.2. Sartorius Proline fixed

volume pipettes

Single channel pipettes:

2.3. Sartorius pipette tips

Sartorius pipette tips are recommended for use

with Proline® Plus pipettes. The use of fully

compatible tips will ensure the maximal pipetting

accuracy and precision, and thereby guarantee

the performance specifications given for the

pipette. Sartorius tips are made of pure virgin

polypropylene and manufactured in protected

clean room conditions.

Sartorius offers a full range of standard Optifit,

and SafetySpace™ Filter tips. Sartorius non-filter

tips are available in trays, bulk packages and

space-saving refill systems. Sartorius non-filtered

tips and trays are autoclavable at 121°C (252F),

Cat. No. Volume Range/

µl

Increment /

µl

Tip/

µl

Safe-Cone Filters

50 pcs/bag

Standard

720210 8-ch 0.50-10 01 10 -

720220 8-ch 5-50 0.5 200, 350 721014

720240 8-ch 50-300 5350 721014

720310 12-ch 5-10 0.1 10 -

720320 12-ch 5-50 0.5 200, 350 721014

720340 12-ch 50-300 5350 721014

Cat. No. Volume

Range/µl

Tip/

µl

Safe-Cone Filters

50 pcs/bag

Standard Plus

722001 510, 200 - -

722004 10 10, 200 - -

722010 20 200, 350 721008 721018

722015 25 200, 350 721008 721018

722020 50 200, 350 721008 721018

722025 100 200, 350 721007 721017

722030 200 200, 350 721007 721017

722035 250 1000 721006 721016

722040 500 1000 721006 721016

722045 1000 1000 721006 721016

722050 2000 5000 721005 721015

722055 5000 5000 721005 721015

20 min, 1 bar (15 p.s.i). All Single Tray Racks and

clean Refill Packs are certified RNase, DNase and

endotoxin-free.

For more information, please go to www.sartorius.

com or contact your local Sartorius support.

3. Unpacking

The pipette package contains the following items:

1. Pipette

2. Calibration/Opening tool

3. Grease

4. Instructions for use

5. Pipette holder

6. Tip

7. Filters (pipettes > 10 µl)

8. Performance certificate according to ISO

8655-6

4. Installing the pipette

holder

For convenience and safety always keep the

pipette vertically on its own holder when not in

use. When installing the holder, please follow the

instructions below:

1. Clean the shelf surface with ethanol.

2. Remove the protective paper from the

adhesive tape

3. Install the holder as described.

(Make sure the holder is pressed against the

edge of the shelf.)

4. Place the pipette onto the holder.

NOTE: An ergonomic, convenient carousel stand

for 6 Proline pipettes is also available (Cat.

No.725600).

5. Pipette Materials

Proline Single Channel

Push button Push button

rod (ABS) (Operating button)

(PA)

Grip Cover

(PA) Tip ejector

(PA)

Handle

(ASA/PC)

Display cover

(PC)

Tip Ejector

collar (PA)

Tip cone

2.5–1000 µl (PVDF)

2000–5000 µl (PPS)

Push button Push button rod

(ABS) (PA)

Grip cover

(PA) Tip Ejector

(PA)

Handle

(ASA/PC)

Display cover

(PC)

Tip cone

housing

(ASA/PC)

Tip cone,

soft sealing

Tip ejector (PVDF)

bar (PETP) (TPU)

(PVDF)

Hard tip

cone

(PC)

Proline Multichannel

6. Operating the pipette

6.1. Volume setting

The volume of the pipette is clearly shown through

the handle display window. The delivery volume

(variable volume pipettes only) is set by turning

the operating button clockwise or anticlockwise.

When setting the volume, please make sure that:

1. The desired delivery volume clicks into place.

2. The digits are completely visible in the display

window.

3. The selected volume is within the pipette’s

specified range.

Do not turn the operating button outside the

volume range because it may jam the mechanism

and damage the pipette.

6.2. Sealing and ejecting tips

Sartorius tips are recommended for use with

Proline pipettes. Before fitting a tip ensure that

the pipette tip cone is clean. Press the tip on the

cone of the pipette firmly to ensure an airtight

seal. The seal is tight when a visible sealing ring

forms between the tip and the black tip cone.

Each pipette is fitted with a tip ejector to help

eliminate the safety hazards associated with

contamination. The tip ejector needs to be pressed

firmly downwards to ensure proper tip ejection.

Make sure that the tip is disposed of into a

suitable waste container.

6.3. Two-in-one pipettes

The Proline fixed volume pipettes 5 and 10 µl, and

variable volume pipettes 0.5-10 µl can be used

with both 10 µl and 200 µl tips. The pipettes are

supplied with two ejector collars and are factory

fitted with the ejector suitable for 10 µl tips.

When using 200 µl tips, please change the ejector

collar by following the instructions below:

1. Push the tip ejector down (1.).

2. Push the opening tool pin between the

ejector bar and ejector collar to release the

locking mechanism (2.).

3. Pull the ejector collar off (3.).

4. Install the ejector collar by pushing the collar

locking pin into the ejector bar hole, keeping

the tip ejector pushed down.

5. Release the tip ejector and test that it runs

smoothly.

6.4. Protective filters

The new tip cones of Sartorius pipettes (>10

µl) allow the use of a exchangeable filter as an

option. The filter prevents liquids and aerosols

from entering the pipette. Two types of filters are

available: a Safe-Cone Filter Standard and a Safe-

Cone Filter Plus (see cat.no. in Chapter 2.1). Plus

filters block the passage of liquids completely. The

filters do not affect the calibration of the pipette.

NOTE: Change the filter on the tip cone regularly

(after 50-250 pipettings).

7. Pipetting techniques

1. Make sure that the pipette, tips and the liquid

are at the same temperature.

2. Make sure that the tip is firmly attached to

the tip cone.

3. Hold the pipette vertically when aspirating

the liquid and place the tip only a few

millimetres into the liquid.

4. Prerinse the tip before aspirating the liquid

by filling and emptying the tip 5 times. This is

important especially when dispensing liquids

which have a viscosity and density different

from water.

5. Always control the operating button

movements with the thumb to ensure

consistency.

7.1. Forward pipetting

This technique employs the blow-out function

ensuring complete delivery of the liquid.

1. Depress the operating button to the first stop.

2. Place the tip(s) just under the surface of the

liquid (2-3 mm) and smoothly release the

operating button up to the starting position.

Carefully withdraw the tip from the liquid,

touching against the edge of the container

to remove excess.

3. Liquid is dispensed by gently depressing the

operating button to the first stop. After a

short delay continue to depress the operating

button to the second stop (= blow-out). This Starting position

procedure will empty the tip(s) and ensure

accurate delivery.

4. Release the operating button to the starting

position. If necessary change the tip(s) and

continue pipetting.

7.2. Reverse pipetting

A selected volume plus an excess is aspirated

into the tip. The delivery is done without blow-

out, and so the excess volume remains in the tip.

The reverse technique is recommended for high

viscosity, biological or foaming liquids, or very

small volumes of liquid.

1. Depress the operating button all the way to

the second stop. Place the tip(s) just under the

surface of the liquid (2-3 mm) and smoothly

release the operating button up to the

starting position.

2. Withdraw the tip(s) from the liquid touching

against the edge of the container to remove

excess.

3. Deliver the preset volume by smoothly

depressing the operating button to the first

stop. Hold the operating button at the first

stop. The liquid that remains in the tip(s)

should not be included in the delivery.

4. The remaining liquid should now be discarded

with the tip(s) or delivered back into the

container vessel.

NOTE: If reverse pipetting technique is used the

pipette might need recalibration.

8. Storage

When not in use it is recommended that your

Proline pipette is stored in a vertical position. See

Installing the pipette holder (Chapter 4).

9. Testing the

performance and

recalibration

It is recommended to check the performance of

your pipettes regularly (e.g. every 3 months) and

always after in-house maintenance. However,

the user should establish a regular testing

routine for their pipettes with regard to accuracy

First stop

Second stop

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