Sakura Tissue-Tek Glas Manual de usuario

Coverslipping

Tissue-Tek® Glas™ Coverslipper

Quick Reference Guide

Tissue-Tek® Glas™ Coverslipper

Sakura Finetek Europe B.V. | Flemingweg 10a, 2408 AV Alphen a/d Rijn | NL

Phone: +31 (0) 88 592 00 00 | Fax: +31 (0) 88 592 00 01 | sakura@sakura.eu | www.sakura.eu

Start Up

The Tissue-Tek®GlasTM Coverslipper is a system that dispenses mounting medium onto stained

histopathological and cytopathological samples on glass slides as well as coverslipping these samples

using cover glasses.

Loading door

Adjustable

Feet level

Coverglass

holder

Available in 40,

50, 55 and 60

Display Panel

Debris tray

Mounting medium

bottle

Receiving racks

Tissue-Tek® Glas™ Coverslipper

Coverslipping

Quick Reference Guide

Tissue-Tek® Glas™ Coverslipper

page 2 | 6 QRG | Glas Coverslipper | 2011 | V-2

Preparation before cover slipping

Hardware

Placing cover glasses

1. Put cover glass holder in place.

2. Make sure the tab of the cover glass holder fits

in the holder (check on the CG size).

Placing coverglasses

Placing mounting medium

When setting the mounting medium bottle for the

first time, you must fill the discharging channel

with mounting medium.

1. Place the waste bottle below the dispensing

nozzle.

2. Fill the priming bottle with mounting medium.

3. Connect the tip of the introduction tube with

the priming bottle.

4. Squeeze the priming bottle to feed the

mounting media until it reaches the dispensing

nozzle.

5. Remove the priming bottle from the

introduction tube.

6. Set the mounting medium bottle.

7. Tilt the mounting medium tube towards the

front and lift the tube.

8. In order to prevent air bubbles, insert the tube

gently into the bottle.

9. Perform an [initial priming] page 2.7 OM.

10. Press [EMER. STOP] to enter the set up

menu.

11. Highlight [1. Prime Dispenser].

12. Press [Verify].

13. Press [Start] and again [Start] to prime.

Changing mounting medium brands

Since mounting mediums vary in viscosity and

formulation, it will be necessary to follow the same

priming procedure when changing to a different

brand of mounting medium.

Follow the detailed instructions written in the

Operating Manual, “priming the instrument”

beginning on page 2.7.

Placing mounting medium Priming bottle connected

to introduction tube

Cover glass holder

Holder hole

Cover glasses

Introduction tube

Coverslipping

Tissue-Tek® Glas™ Coverslipper

Quick Reference Guide

Tissue-Tek® Glas™ Coverslipper

QRG | Glas Coverslipper | 2011 | V-2 page 3 | 6

Installing the nozzle reservoir

1. Set the dispensing tray in the system by

aligning the tray with the two guide rails (only

for the first time use).

Placing Anti-drying Bottle (1) and waste Container (2

Filling anti drying bottle

1. Fill the anti drying bottle with solvent to just

below the lid.

Note: type of solvent depends on the mounting medium

2. Place the anti-drying bottle and waste

container in the dispensing tray.

Handling the dispensing nozzle

1. When setting the anti-drying bottle, push down

the dispensing nozzle in a backward direction

and then set the bottle.

2. After the anti-drying bottle and waste container

have been set, lift the dispensing nozzle and

return the nozzle so that its tip enters the hole

in the anti-drying bottle. (see description

“handling the dispensing nozzle”).

Handling dispensing nozzle Filling anti-drying bottle

Filling “pump anti drying bottle”

1. Remove the lid and add solvent for mounting

medium into the pump reservoir.

Note: type of solvent depends on the mounting medium.

2. Fill the pump anti drying bottle with solvent to

just below the lid.

Filling pump anti drying bottle

Filling loading station

1. Fill the loading station with solvent until the

specified level.

Note: type of solvent depends on the mounting medium.

Filling loading Station

Required level

Tissue-Tek® Glas™ Coverslipper

Coverslipping

Quick Reference Guide

Tissue-Tek® Glas™ Coverslipper

page 4 | 6 QRG | Glas Coverslipper | 2011 | V-2

Preparation before coverslipping

Software

VOLUME KNOB

Can be used to fine tune the volume of mounting

medium dispensed on the slide.

SPEED KNOB

Refers to the speed at which the coverglass is

applied to the slide.

STOP

Can be used to stop the instrument while operating.

TEST

Can be beneficial if the operator would like to test

one slide.

SIZE

Toggles between coverglass sizes 40, 50, 55 and 60

mm.

VERIFY

Key acts as an enter key.

PRIMING FUNCTION

Can be used to verify proper dispensing.

When “single key”is pressed mounting medium

will be dispensed from the nozzle one time.

When “continuous”key is pressed the mounting

medium will dispensed continuously until the

“STOP” key is pressed.

Control panel

Coverslipping Operation

Cover slipping slides

Pre-operational checks

1. Turn on POWER.

2. Remove the loading station lid.

3. Check the solvent volume level in anti-drying

bottle and dispensing pump reservoir.

4. Clean any possibly spilled mounting medium.

5. Place receiving racks in the unloading area.

6. Put coverglass (CG) holders in place and fill

with coverglasses.

7. Check if CG size on screen matches CG in

holder.

8. Check the volume level of mounting medium.

Start of operation

1. Check CG size, coverslipping speed and volume

of mounting medium.

2. Select coverslipping program.

3. Press and hold [prime] key for 3 seconds

4. Pull out the loading station and set baskets.

Close loading station door and [start]

coverslipping.

Check CG size

Insert slides in parallel

Do not tilt slides

Loading of the slides

Set by making sure the

“UP SIDE” marks are

facing the right.

Loading of the baskets

Coverslipping

Tissue-Tek® Glas™ Coverslipper

Quick Reference Guide

Tissue-Tek® Glas™ Coverslipper

QRG | Glas Coverslipper | 2011 | V-2 page 5 | 6

Maintenance

(section 5 operating manual)

Daily

Station cleaning

1. Clean each station and its surrounding area.

2. Brush off each station, remove dust and/or

glass particles.

3. Using a cloth moistened with solvent for

mounting medium*, remove any mounting

medium attached to the stations.

Cover glass and Slide glass arm cleaning

1. Clean the suction pads using a cloth moistened

with 100% alcohol.

End of Operation

1. Remove receiving racks.

2. Remove empty baskets.

3. Place dispensing nozzle in the lubrication bottle

above the solvent (1)! If the instrument will not

be used for more than one week, place the

dispensing nozzle in the solvent (2) by pulling

the lever towards you.

Power off

1. Turn off power and place the lid on the loading

station.

Mounting medium nozzle cleaning

1. Clean the mounting medium nozzle by using a

cloth moistened with solvent for mounting

medium*.

2. At the end of every day before switching off the

machine, place dispensing nozzle in the anti-

drying bottle above the solvent!

Weekly

Waste container

1. Remove waste container and replace it with a

clean container.

2. Check the spatula part.

Soak the used waste container for 24 hours in

solvent for mounting medium*.

Cleaning the loading station

Lever

Station cleaning

Suction pad Slide glass arm

Suction pad coverglass arm

1Normal state

2Dispensing nozzle

down lever is pulled

Lever

Tissue-Tek® Glas™ Coverslipper

Coverslipping

Quick Reference Guide

Tissue-Tek® Glas™ Coverslipper

page 6 | 6 QRG | Glas Coverslipper | 2011 | V-2

6 Months

Anti-drying bottle

1. Change the solvent for mounting medium*in

the anti-drying bottle.

Cleaning the receiving racks

1. Soak the racks for 24 hours in solvent for

mounting medium*.

Instrument strainer

Open cover on the right side of the instrument and

remove the strainer cap. Use xylene soaked brush

to clean the filter. Replace the cap.

Yearly

1. Preventive maintenance by a Sakura

representative or a qualified service engineer.

Trouble shooting

(section 6 operating manual)

The trouble shooting part of the operating manual

is divided into three sections to explain how to

recover from conditions that may occur during

routine operation.

In the first section,

Troubleshooting Chart is provided, which identifies

possible conditions related to the electrical and

mechanical operations.

The second section,

Messages, explains specific messages that may be

displayed during routine operation in detail.

The third section,

Gives a detailed explanation of all error codes and

how to recover from them.

Operating manual section 6, trouble shooting

1. Change the solvent for mounting medium*in

the loading station.

*Solvent for mounting medium is the reagent which is compatible

with the mounting medium, Tissue clear when using Tissue Mount

and Xylene when using Glass coverslipping medium.

Waste container, spatula part

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