Promega Maxwell 16 MDx Manual de usuario

Revised 4/15

TM320

OPERATING MANUAL

Maxwell®16

MDx Instrument

Instrucons for Use of Product

AS3000

2800

Woods Hollo

w Rd.

Madison, WI USA

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 1

www.promega.com TM320 · Revised 4/15

All technical literature is available at: www.promega.com/protocols/

Visit the web site to verify that you are using the most current version of this Technical Manual.

E-mail Promega Technical Services if you have questions on use of this system: [email protected]

Maxwell®16 MDx

Instrument Operating Manual

1. Introduction............................................................................................................................................3

1.A. Maxwell®16 MDx Puriﬁcation Procedure.........................................................................................3

1.B. Maxwell®16 MDx Instrument Speciﬁcations .................................................................................... 4

1.C. Product Components and Symbol Key ..............................................................................................5

1.D. Inspection ....................................................................................................................................... 6

1.E. Precautions .....................................................................................................................................7

1.F. Environmental Requirements (operating, shipping and storage conditions)........................................9

2. Hardware Overview .............................................................................................................................. 10

3. Hardware Mode .................................................................................................................................... 13

4. Unpacking and Setting Up the Maxwell®16 MDx Instrument.............................................................. 14

4.A. Unpacking..................................................................................................................................... 14

4.B. Removing the Magnetic Rod Assembly/Plunger Bar Platform Shipping Anchors .............................. 14

4.C. UV Bulb Installation ...................................................................................................................... 15

4.D. Maxwell®Sample Track Software................................................................................................... 16

4.E. Operational Setup.......................................................................................................................... 16

5. Operating the Maxwell®16 MDx Instrument ....................................................................................... 23

5.A. Preprogrammed Methods .............................................................................................................. 23

5.B. Setting Up Samples and Collecting Input Data ................................................................................ 25

5.C. Performing a Sample Puriﬁcation Run............................................................................................ 29

5.D. Post-Run UV Light Treatment........................................................................................................ 30

6. Run Reports .......................................................................................................................................... 31

6.A. Data File Transfer at the End of a Run ............................................................................................ 31

6.B. Data File Transfer at a Later Time .................................................................................................. 32

6.C. Data File Management................................................................................................................... 32

7. Changing Conﬁguration Parameters ..................................................................................................... 32

7.A. Changing the Hardware ................................................................................................................. 33

7.B. Changing the Instrument Firmware Conﬁguration .......................................................................... 36

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TM320 · Revised 4/15 www.promega.com

8. Cleaning and Maintenance ................................................................................................................... 37

8.A. General Care ................................................................................................................................. 37

8.B. Cleaning the Hardware ..................................................................................................................38

8.C. Dealing with Spills......................................................................................................................... 38

8.D. Decontamination Using the UV Lamp............................................................................................. 38

8.E. Replacing the UV Bulb................................................................................................................... 39

8.F. Periodic Maintenance .................................................................................................................... 39

9. Troubleshooting.................................................................................................................................... 40

10. Appendix............................................................................................................................................... 45

10.A. User-Deﬁned Methods ................................................................................................................... 45

10.B Homogenization Method ............................................................................................................... 52

10.C. Updating Firmware........................................................................................................................ 53

10.D. Service .......................................................................................................................................... 53

10.E. Instrument Return......................................................................................................................... 55

10.F. Instrument Disposal ...................................................................................................................... 56

11. Warranties, Service Agreements and Related Products ........................................................................ 57

11.A. Warranty Information.................................................................................................................... 57

11.B. Warranty and Service Agreement Options....................................................................................... 58

11.C. Related Products ........................................................................................................................... 60

12. Certiﬁcate of Decontamination.................................................................................................... 61

13. Summary of Changes .................................................................................................................. 62

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www.promega.com TM320 · Revised 4/15

1. Introduction

1.A. Maxwell®16 MDx Puriﬁcation Procedure

The Maxwell®16 MDx Instrument(a) provides automated nucleic acid puriﬁcation methods for multiple analytes.

The methods use sample lysis and binding to paramagnetic particles as the primary separation principle. It has

two modes for diﬀerent elution volumes. The Standard Elution Volume (SEV) mode allows elution in a volume of

up to 400µl. The Low Elution Volume (LEV) mode allows elution in as little as 25µl. Up to 16 samples can be

prepared in a single run.

The automated steps performed by the Maxwell®16 MDx Instrument include:

• Sample lysis in the presence of a chaotropic agent, detergent and/or alcohol

• Binding of nucleic acids or proteins to paramagnetic particles

• Washing of the target molecules bound to the particles away from other cellular components

• Elution of the product

The instrument contains a touch screen for navigating, programming and running the instrument. It has a UV

lamp to aid with decontamination. The Maxwell®16 MDx Instrument, in association with the Maxwell®Sample

Track Software, has the ability to record and provide run data. It has a USB port that can be used to attach a bar

code reader, allowing sample and reagent information to be entered using bar codes. The Maxwell®16 MDx

Instrument can report the data gathered for each run, and the report can be sent to a serial printer or to a

computer.

The user selects the protocol to be run, collects the selected bar code information (optional) and places the

samples into the reagent cartridges. The cartridges are placed into the machine and the door shut to start the run,

which automatically performs the protocol.

The temperature of the samples is regulated by a heating system that is controlled by the protocol.

Maxwell®Sample Track is a communications program that allows downloading and printing of the run data

(see Technical Manual #TM314).

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TM320 · Revised 4/15 www.promega.com

Maxwell®16 MDx Instrument Features

• Easy-to-use and easy-to-maintain system operation that standardizes nucleic acid or protein sample

preparation workﬂow

• Comprehensive technical support

• System controlled via multi-language LCD readout

• UV lamp to aid in decontamination of instrument

• Reporting functionality

• Maxwell®Sample Track Software and bar code reader included

• Preprogrammed methods for DNA, RNA and protein puriﬁcation

• Ability to create user-deﬁned protocols

1.B. Maxwell®16 MDx Instrument Speciﬁcations

Processing Time: Depending upon sample type and method used, 20–50 minutes

Number of Samples: Up to 16

Weight: 42.7lb (19.4kg)

Dimensions (W ×D ×H): 12.8 ×17.3 ×12.9 inches (325.5 ×438.2 ×326.5mm)

Power Requirements: 100–240VAC, 50–60Hz, 2.1A

Fuse: 3A time-lag fuse

UV Bulb: Average lifetime approx. 3000 hours, length 134.5mm,

diameter 15.5mm, 4.5W, 0.17A current, 29V, Spectral Peak 253.7,

UV output 0.8W

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 5

www.promega.com TM320 · Revised 4/15

1.C. Product Components and Symbol Key

PRODUCT CAT.#

Maxwell® 16 MDx Instrument AS3000

For Research Use Only. Not for Use in Diagnostic Procedures. Includes:

• 1 Maxwell®16 MDx Instrument

• 1 Power Cable

• 1 UV Bulb

• 1 SD Card

• 1 SD Card reader package (contains SD card reader and cable)

• 1 CD containing the technical manual

• 1 Quick Start Guide

• 1 Bar Code Reader (provided in a separate box)

• 1 CD containing Sample Track Software and manual

• 1 RS-232 Cable for communication with a computer or printer

• 1 RS-232/USB Adaptor for data export to computer

• 1 SEV Cartridge Rack

• 1 SEV Magnetic Elution Rack

• 1 SEV Magnetic Rod Assembly

• 1 LEV Cartridge Rack

• 1 LEV Magnetic Rod and Plunger Bar Adapter*

*The Maxwell®16 MDx Instrument includes both the SEV and LEV hardware. The instrument will be

provided conﬁgured in the LEV mode, with the LEV Magnetic Rod and Plunger Bar Adapter installed.

Symbol Key

Symbol Explanation Symbol Explanation

Important Catalog number

2800

Woods Hollo

w Rd.

Madison, WI USA

Manufacturer Serial number

6 Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516

TM320 · Revised 4/15 www.promega.com

1.D. Inspection

Upon receiving your Maxwell®16 MDx Instrument, please inspect the package carefully to make sure all accesso-

ries are present. Standard accessories are shown in Figure 1.

8090TA

11470TA

11546TA

Figure 1. Maxwell®16 MDx Instrument accessories. Panel A. Components shown include: Bar Code

Reader Cable, Bar Code Reader, Power Cord, UV Bulb, SD Card Reader and Cable, SD Card, RS-232/USB

Adaptor Cable, RS-232 Cable. Not pictured: Quick Start Guide, CD containing Technical Manual and CD

containing Sample Track Software. Bar Code Reader and Bar Code Reader cable are provided in a separate box.

Panel B. LEV Cartridge Rack, LEV High-Strength Magnetic Rod, Plunger Bar Adapter. Panel C. SEV Cartridge

Rack, SEV Elution Rack, SEV Magnetic Rod.

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 7

www.promega.com TM320 · Revised 4/15

1.E. Precautions

IMPORTANT SAFETY INSTRUCTIONS. SAVE THESE INSTRUCTIONS.

Safety Symbols and Markings

Danger. Hazardous voltage. Risk of electrical shock.

Warning. Risk of personal injury to the operator or a safety hazard to the instrument or

surrounding area.

Warning. Pinch point hazard.

Warning. Hot surface. Burn hazard.

Warning. Lifting hazard.

Warning. Biohazard.

Warning. UV light hazard. Do not look directly at the UV light.

Warning. Wear gloves when handling.

Warning. It is important to understand and follow all laws regarding the safe and proper

disposal of electrical instrumentation. Please contact your local Promega Representative for

disposal of the instrument. Please follow your institutional requirements for disposal of the

accessories.

5294MA.eps

5295MA.eps

6422MA

8252MA

6687TA.eps

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TM320 · Revised 4/15 www.promega.com

1.E. Precautions (continued)

• Changes or modiﬁcations to this unit not expressly approved by the party responsible for compliance

could void the user’s authority to operate the equipment.

• This equipment has been designed and tested to CISPR 11 Class A. In a domestic environment it may

cause radio interference, in which case you may need to take measures to mitigate the interference.

• Do not use this device in proximity to sources of strong electromagnetic radiation (e.g., unshielded

intentional RF sources), as these may interfere with the proper operation.

• Do not use this instrument for anything other than its intended use.

• Always disconnect the power before cleaning or performing routine maintenance.

• Do not disassemble unit.

• Do not override the door sensor. Moving parts or potential exposure to UV light from the bulb may

cause personal injury.

• Ensure cartridges, elution tubes and plungers have been securely inserted in their correct positions and

orientation. Failure to do so may result in damage to the instrument.

• After each run, verify that the plungers have been completely removed from the magnet rods before

pressing Run/Stop to extend the platform.

• Use only Promega Maxwell®16 cartridges and plungers designed for use with the appropriate hardware

conﬁguration of the instrument.

• Do not reuse cartridges, plungers or elution tubes

• Wear gloves when handling the bulb, for example during insertion and removal of the bulb. Do not

operate the UV lamp if the instrument door is open.

• The UV bulb contains mercury and must be disposed of properly. To dispose of a bulb, please follow

your institutional requirements for cleanup and disposal of mercury.

• Use only Promega supplied UV bulbs (Cat.# SP1080).

• If the equipment is used in a manner other than that speciﬁed by Promega, the protection provided by

the equipment may be impaired.

• Keep hands clear of instrument platform as it moves in and out of the instrument.

• During elution, the heated elution block at the front of the platform becomes very hot. Do not touch.

• To avoid muscle strain or back injury, use lifting aids and proper lifting techniques when removing or

replacing the instrument. The Maxwell®16 MDx Instrument weighs 42.7lb (19.4kg) and should be

handled by two people.

• Equipment can be hazardous due to the use of chemical and biohazardous substances

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 9

www.promega.com TM320 · Revised 4/15

1.F. Environmental Requirements (operating, shipping and storage conditions)

Power Requirements: 100–240VAC, 50–60Hz, 2.1A

Temperature: 5–40°C

Humidity: Up to 80% relative humidity (for operation, shipping and storage)

The Maxwell®16 MDx Instrument is intended for indoor use only. Wipe up spills immediately. Install the

instrument on a clean, level surface. To avoid shortening the expected lifespan of the instrument, install in a

location that meets the following criteria:

• Locate on a sturdy, level surface.

• Avoid dusty areas.

• Choose a location that has good air circulation and is not exposed to direct sunlight.

• Avoid noisy electrical power sources (e.g., power generators).

• Do not install in a location where there is large temperature variability or high humidity.

• Position the instrument so that it is easy to unplug from the power source.

• Do not place next to heat sources.

• Do not use near ﬂammable gases or liquids.

• Do not place near other electrically sensitive instruments.

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