Procomcure Phoenix-Pure96 Manual de usuario
Phoenix-Pure96
Nucleic Acid Purification System
Operation Manual
v1.0
2
Foreword
Thank you for purchasing the Phoenix-Pure 96 Nucleic Acid Purification System. This
manual describes the function and operation of then instrument. In order to use the
instrument properly, please read this manual carefully before operating the device.
Keep it with your device in case you encounter handling issues while operating.
Opening Check
Please check the instrument and accessories according to the packing list when you
first open the packing case. If anything is wrong or missing, please contact
Procomcure Biotech GmbH
Breitwies 1
A-5303 Thalgau
+43 6229 39608
support@procomcure.com
www.procomcure.com
Safety Warnings and Guidelines
1. Important information for safe use
Users should have a clear understanding of how to use this instrument before
operation, please read this manual carefully prior to operation. Any improper
operation may cause injury or electric shock.
Please read the manual carefully and operate safely according to the guidelines.
2. Security
The operation and maintenance and of the instrument should comply with the basic
guidelines and warnings below. Incorrect operation or maintenance will have effect
on using life, performance, and safety features of the instrument.
•The instrument is a normal indoor instrument which conforms to class I of GB
4793.1 standard. Please read this manual carefully before operation.
•The device must be used by experienced personnel with appropriate training.
•The operator should not repair the instrument in case any injury or out of
warranty. If service required, please contact Procomcure Biotech for repair.
•Before powering on, please make sure the voltage of the power supply is
consistent with the required voltage. Make sure the rated load of the power
outlet is not less than required by the instrument.
•If the power cord is damaged, replace it with the same type and specification
power cord.
•Do not cover anything on the instruments when using.
•Do not touch the heating bock during operation to avoid injury.
3
•The instrument should be kept in an area with minimal dust, away from wet
areas and direct sunlight. Additionally, the installation location should have
sufficient ventilation and be protected
•from electromagnetic interference and heat sources.
•Do not cover the vent of the instrument as this can cause overheating.
•When several instruments are used at the same time, the distance between
each instrument should be more than 100 cm.
•Power off when not in use. If the instrument will not be used for a long period of
time, cover it with a cloth or plastic to protect it from dust.
•Disconnect the power cord from the jack at once in the following cases, and
contact Procomcure Biotech:
oLiquid enters the instrument.
oInstrument was rained or watered.
oAbnormal operation: such as abnormal sound or smell.
oInstrument dropping or outer shell damaged.
oThe function has obviously changed.
3. Maintenance
The instrument should be cleaned regularly using a soft cloth damp with a small
amount of alcohol. If any stain on the surface of the instrument, wipe it with soft cloth
damp with cleansing cream.
4. Transportation and Storage Requirements
Ambient temperature: 10°C ~ 35°C
Relative humidity: ≤ 70%
Atmosphere pressure range: 500 ~ 1060 hpa
Locate it in a well-ventilated room, away from corrosive gas.
4
Contents
Chapter 1 Introduction........................................................................................... 5
1. Application.................................................................................................................. 5
2. Contraindication........................................................................................................ 5
3. Service Life................................................................................................................... 5
Chapter 2 Specifications ....................................................................................... 5
1. Working Conditions.................................................................................................... 5
2. Basic Parameters........................................................................................................ 5
3. Overall Dimensions..................................................................................................... 6
Chapter 3 Basic Operating Instructions ............................................................... 6
1. Structures...................................................................................................................... 7
2. Touch Screen .............................................................................................................. 9
Chapter 4 Operations ............................................................................................ 9
1. Power Connection..................................................................................................... 9
2. Kits Installation............................................................................................................. 9
3. Detailed Operations ................................................................................................10
3.1. Start-up Interface......................................................................................10
3.2. Run Program Interface ............................................................................10
3.3. Manage Program.....................................................................................16
3.4. System Settings..........................................................................................20
3.5. UV Sterilization ...........................................................................................25
3.6. Help..............................................................................................................25
Chapter 5 Trouble Shooting................................................................................. 26
1. Troubleshooting ........................................................................................................26
2. Software Error Alarm List..........................................................................................27
Chapter 6 Accessory ........................................................................................... 28
Chapter 7 Abbreviations and Tags..................................................................... 28
1. Abbreviations............................................................................................................28
2. Tags .............................................................................................................................28
5
Chapter 1 Introduction
Phoenix-Pure96 Nucleic Acid Purification System is a newly launched automatic
extraction and purification system for DNA/RNA, proteins and cells. It can absorb,
transfer and release magnetic beads by magnetic rod and magnetic rod sleeve to
separate magnetic beads and samples. The operation is automatic, fast, and simple.
Users can extract 1 to 96 samples simultaneously with special kits. Phoenix-Pure96 can
extract samples of animal/plant tissue, blood and body fluids, etc with different kinds
of magnetic bead nucleic acid extraction reagents. It is mainly used for the extraction
and purification of nucleic acid from human body samples.
1. Application
This instrument is suitable for the extraction and purification of nucleic acids in animal
and plant tissues, blood and body fluids and other samples (mainly used in human
body samples).
2. Contraindication
No contraindication.
3. Service Life
Service life of the instrument is five years.
For production date, please see the label on back of the instrument.
Chapter 2 Specifications
1. Working Conditions
Environmental Temperature: 10°C -35°C
Relative Humidity: ≤ 70%
Input Voltage: AC 100-240V, 50Hz/60Hz
2. Basic Parameters
Instrument
Phoenix-Pure 96
Principle
Magnetic Particle Method
Sample Volume
50 μL—1000 μL
Throughput
96
Stability
CV ≤5%
Extraction time
10 ~ 60 min/time
Temperature control module
Room temperature to 120°C for lysis and elution
Temp. Accuracy
±1°C
6
Vibrate and mix
10 different speeds
Operation
7-inch color touch screen, mouse can be connected
Programs
8 groups of programs can be preset, and can store 100
groups of programs
Program management
Including create, edit, delete and protocol mode
Extension interface
With USB port and Ethernet port
Network
Extended Ethernet remote control, WiFi function.
Power Supply
AC100-240V, 50Hz/60Hz, 250VA
3. Overall Dimensions
Chapter 3 Basic Operating Instructions
This chapter mainly introduces structures, basic operation keys, displays as well as
preparations before starting up. Please read this chapter carefully before using this
instrument.
7
1. Structures
1.1. Front
1.2 Back
8
1.3. Cabin Door
The cabin door of Phoenix-Pure96 can be opened which is convenient for cleaning
and maintenance.
1.4. Transparent Cover
The transparent cover on the right side of the instrument is for placing or taking out kits.
The cover can be removed to make it compatible with automatic liquid transfer
workstations.
9
2. Touch Screen
Display screen: Touch screen, mouse also can be connected for operation.
TAB: Select shortcut program.
RUN: Start the shortcut program and run the instrument.
STOP: Stop the operation.
Chapter 4 Operations
1. Power Connection
AC 100 - 240V
2. Kits Installation
Open the cabin door, put kits on the plate position of the rotary table, press position
button to turn the rotary table and place all the kits in turn.
10
3. Detailed Operations
3.1. Start-up Interface
Turn on the instrument and make sure the door is closed before start. The start-up
interface will come up.
Then, it will enter “Run Prog.” interface.
3.2. Run Program Interface
This interface including two modes: “shortcut” mode and “list mode”, as shown in
below Fig 9 and Fig 10.
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